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Citations for PerkinElmer :
101 - 150 of 263 citations for Rabbit Anti ALB Recombinant Antibody Clone CA645 ; scFv Fragment since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... 10 mg of purified GST-fusion protein (unedited Cpx7AI125,N130 or edited Cpx7AI125M,N130S) was used per reaction and incubated with 2,500 units of recombinant kinase and [32P]ATP (Perkin Elmer). Reaction products were separated by SDS-PAGE and gels were stained with Bio-Safe Coomassie Blue (Bio-Rad) ...
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bioRxiv - Biochemistry 2023Quote: ... was mixed in 20 µl of the cytoplasmic buffer with 2- to 10-fold excess of recombinant Sm proteins and each sample was supplemented with 2 µCi of 3H-labeled SAM (PerkinElmer) for overnight methylation at 32 °C ...
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Wnt-dependent activation of ERK mediates repression of chondrocyte fate during calvarial developmentbioRxiv - Developmental Biology 2021Quote: ... The slides were then washed in 1X TBS-T and incubated in HRP-conjugated goat-anti-rabbit secondary (1:1500, PerkinElmer NEF812001EA, Akoya Biosciences) for 45 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... sections were washed in TBST and incubated with Alexa Fluor® 594 AffiniPure Goat Anti-Rabbit IgG (H+L) (Abacus DX, Australia) with Spectral DAPI (FP1490) added (1/1000) (Perkin Elmer, USA) for one hour at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... incubated 1h in blocking buffer and then incubated overnight with an anti-DNP-POD antibody (1:1000, Perkin Elmer). The next day embryos were washed in PBS-Tween ...
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bioRxiv - Cell Biology 2023Quote: ... 10 nM full-length 6x-His-tagged human G3BP1 produced in mammalian cells was added to the reaction mix containing test compound and 0.5 nM anti-His-Tb antibody (PerkinElmer). Assays were initiated by adding 4 nM FAM-labeled PEG6-USP10 24-mer peptide probe (FAM-PEG6-GALHSPQYIFGDFSPDEFNQFFVT ...
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bioRxiv - Developmental Biology 2023Quote: ... was used to detect the anti-pH3 antibody after detection of ISH probe by using TSA plus (Perkin Elmer) green (FP1168).
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bioRxiv - Genetics 2021Quote: ... DNA fragments were detected using a mix of five probes labelled by PCR amplification with 32P dCTP (Perkin Elmer) described in Fig S1 ...
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bioRxiv - Bioengineering 2022Quote: Saturation assays for binding of K1K1H6 to recombinant MET-Fc protein were performed in 384-well microtiter plates (OptiPlate™-384, PerkinElmer© ...
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bioRxiv - Cancer Biology 2022Quote: Tritiated cGAMP was synthesized via a bio-catalytic reaction in which recombinant cGAMP synthase preactivated with herring DNA was incubated with [3H]-ATP (Perkin Elmer) and [3H]-GTP (Perkin Elmer ...
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bioRxiv - Neuroscience 2021Quote: ... and Lumi4-Tb cryptate conjugated anti-IP1 antibody (emission at 620 nm) was detected using an Envision plate reader (PerkinElmer). Results were calculated from the 665/620 nm ratio and normalised to the maximum response stimulated by acetylcholine.
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bioRxiv - Developmental Biology 2021Quote: ... Antisense RNA probes labeled with DNP were used in all in situ hybridizations and probe detection was performed using a HRP-coupled anti-DNP antibody (PerkinElmer) and a fluorescein coupled tyramide (Vize et al. ...
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bioRxiv - Neuroscience 2022Quote: ... then incubated for 30 min at 25 °C with shaking at 400 RPM with 9 ng/well of DELFIA Eu-N1 anti-mouse antibody in DELFIA assay buffer (PerkinElmer), washed with 3 × 100 μl of PBST before enhancing with 80 μl of DELFIA Enhancement Solution (PerkinElmer) ...
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bioRxiv - Biochemistry 2022Quote: ... 5 μL time points were taken and quenched with 1.33 mM EDTA then brought to 20 μL final volume for detection using 1 nM LANCE Ultra Europium anti-H3K4me1/2 antibody (TRF0402, PerkinElmer) and 50 nM LANCE Ultra Ulight-Streptavidin (TRF0102 ...
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bioRxiv - Molecular Biology 2021Quote: ... The fragment lengths of the sequencing libraries were verified via micro-capillary gel electrophoresis on a LabChip GX Touch system (PerkinElmer). The libraries were quantified by fluorometry on a Qubit instrument (LifeTechnologies ...
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bioRxiv - Molecular Biology 2023Quote: ... The average fragment size of the final libraries was determined using DNA 1K/12K/Hi Sensitivity Assay LabChip (760517, PerkinElmer)) and DNA High Sensitivity Reagent Kit (CLS760672 ...
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bioRxiv - Immunology 2021Quote: ... Cells were immunostained with DAPI and Alexa488-conjugated anti-N antibody (CR3009; produced in-house) and imaged using an Opera Phenix (Perkin Elmer). The ratio of infected (Alexa488+ ...
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bioRxiv - Genomics 2021Quote: ... the anti-DIG-HRP antibody was detected with the TSA-Cy3 substrate (1:50, TSATM PLUS Cy3 kit, NEL 745001KT, Perkin Elmer) for 10 min at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was probed with a goat anti-mouse antibody conjugated to horseradish peroxidase and bands were visualized using the Western Lighting Plus-ECL reagent (Perkin Elmer). When performing the KatG expression analysis in response to C10 treatment ...
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bioRxiv - Cell Biology 2021Quote: ... Radioactive counts from the media that remain soluble after TCA precipitation (peptide fragments) and pellets from the cells (total non-degraded cell proteins) were measured in a TopCount radioactivity detector (Perkin Elmer). A “protein degradation index” was calculated as the ratio between the counts detected in the soluble media and the counts detected in the pellet cell fraction (82).
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bioRxiv - Immunology 2023Quote: ... Libraries were assessed for concentration and fragment size using the DNA High Sensitivity Assay on the LabChip GX Touch (Perkin Elmer). The resulting libraries were sequenced on a multiplexed paired end 100bp Illumina NovaSeq 6000 S4 flowcell ...
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bioRxiv - Molecular Biology 2023Quote: ... Transcription was initiated with addition of 12 pmol rrnBP1 PCR promoter fragment pre-mixed with 1 mM ATP and 4 μCi α-[32P]-CTP (3,000 Ci mmol−1; Perkin Elmer) at 37 °C ...
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bioRxiv - Genomics 2024Quote: ... DNA concentration and fragment size was conducted using the DNA High Sensitivity Assay on the LabChip GX (Perkin Elmer, Waltham, MA) tool ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated with a horseradish peroxidase-conjugated anti-fluorescein antibody (diluted 1:500 with DIG-1, PerkinElmer, Foster City, CA) for 1 h and washed twice with DIG-1 for 5 min each time ...
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bioRxiv - Immunology 2024Quote: ... Five-micrometer serial sections were stained with a human-specific anti-COXIV antibody and scanned using the Vectra® Polaris Automated Imaging System (PerkinElmer, USA) at 20×magnification ...
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bioRxiv - Microbiology 2024Quote: ... muridarum clone (G5-pGFP-Luci) were imaged using the Xenogen IVIS imaging system (PerkinElmer, Hopkinton, MA) on various days after infection ...
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bioRxiv - Microbiology 2022Quote: ... The fragment size distribution of the libraries was verified via micro-capillary gel electrophoresis on a LabChip GX system (PerkinElmer, Waltham, MA). The libraries were quantified by fluorometry on a Qubit instrument (LifeTechnologies ...
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bioRxiv - Physiology 2022Quote: ... The fragment size distribution of the libraries was verified via micro-capillary gel electrophoresis on a LabChip GX system (PerkinElmer, Waltham, MA). The libraries were quantified by fluorometry on a Qubit fluorometer (LifeTechnologies ...
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bioRxiv - Plant Biology 2022Quote: ... The fragment size distribution of the libraries was verified via micro-capillary gel electrophoresis on a LabChip GX system (PerkinElmer, Waltham, MA). The libraries were quantified by fluorometry on a Qubit fluorometer (LifeTechnologies ...
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bioRxiv - Neuroscience 2023Quote: ... Reactions were initiated by adding 2.5 µM ATP at 27°C and were terminated after 60 min with 10 µL of 2.5X Stop Solution/Detection Mix containing 20mM EDTA and 4nM LANCE Ultra Europium-anti-phospho-PKC (Ala25Ser) peptide antibody (Perkin Elmer, Waltham, MA). After incubation at 27°C for another 60 min ...
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bioRxiv - Microbiology 2023Quote: ... Libraries were assessed for concentration and fragment size using the DNA High Sensitivity Assay on the LabChip GX Touch (Perkin Elmer, Waltham, MA). The library concentrations were also assessed by qPCR using the KAPA Library Quantification Kit (Complete ...
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bioRxiv - Microbiology 2023Quote: ... Cycle sequencing was carried out using both forward and reverse primer of each fragment in a GeneAmp9600 thermal cycler (Perkin Elmer, Waltham, USA) employing 30 cycles at 96°C for 10 s ...
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bioRxiv - Molecular Biology 2020Quote: ... Double-stranded DNA probes were synthesized by random-prime labeling of PCR fragments with [α-32P]dATP and [α-32P]dTTP (PerkinElmer, Waltham, MA). Probe locations are as follows with numbering relative to the start codon of the indicated protein-coding gene ...
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bioRxiv - Cancer Biology 2021Quote: ... Number of basal and suprabasal cells in each clone was counted using Volocity 6 Image Analysis software (PerkinElmer).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 5 μL of 1/100 dilution of the Alexa Fluor® 647-anti cAMP antibody solution in the Detection Buffer (PerkinElmer Life and Analytical Sciences) and 10 μL of 1/5000 dilution of Eu-W8044 labeled streptavidin and Biotin-cAMP in the Detection Buffer (PerkinElmer Life and Analytical Sciences ...
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bioRxiv - Biochemistry 2021Quote: ... 5 μL of 1/100 dilution of the Alexa Fluor® 647-anti cAMP antibody solution in the Detection Buffer (PerkinElmer Life and Analytical Sciences) and 10 μL of 1/5000 dilution of Eu-W8044 labeled streptavidin and Biotin-cAMP in the Detection Buffer (PerkinElmer Life and Analytical Sciences ...
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bioRxiv - Immunology 2024Quote: ... Clone 2C.4 was screened against human cardiac myosin and the β-adrenergic receptor (βAR) enriched membranes (Perkin Elmer) by ELISA ...
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bioRxiv - Immunology 2024Quote: ... All antibodies were obtained from PerkinElmer Life Sciences except for the anti-FLAG-FITC conjugated antibody which was purchased from BioLegend ...
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bioRxiv - Genomics 2021Quote: Two-color FISH experiments were performed using human fosmid (n=2) or BAC (n=2) clones directly labelled by nick-translation with Cy3-dUTP (PerkinElmer) and fluorescein-dUTP (Enzo ...
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bioRxiv - Cancer Biology 2023Quote: ... and positive clones were selected by bioluminescence imaging (BLI) with IVIS Lumina II In Vivo Imaging system (PerkinElmer, Waltham, USA). Ten Luc2 positive clones were pooled to maximize the labeling rate and minimize the colony effect.
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were detected using HRP-linked secondary antibodies together with Western Lightning Plus-ECL (Perkin Elmer). Fiji (ImageJ ...
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bioRxiv - Neuroscience 2020Quote: ... antibodies plus Cy3-TSA (1:200, PerkinElmer). Sections were cover-slipped using Prolong Diamond Antifade Mounting media with DAPI (ThermoFisher ...
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bioRxiv - Cancer Biology 2024Quote: ... blocked in antibody (Ab) Diluent (Perkin Elmer), incubated for 30 min with the primary antibody ...
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bioRxiv - Cell Biology 2023Quote: ... Antibodies were detected via ECL reagents (PerkinElmer).
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bioRxiv - Molecular Biology 2022Quote: ... all PCR products of the known riboswitches as well as those from the clones derived from the SELEX with degenerated TPP riboswitches were radiolabeled with [α-32P] UTP (Perkin Elmer) during in vitro transcription using T7 RNA polymerase for 3 hours at 37 °C (20 μL PCR products [~400 ng] ...
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bioRxiv - Microbiology 2020Quote: A matrix with all antibodies provided by PerkinElmer was tested for the ability to detect NP.
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bioRxiv - Neuroscience 2024Quote: ... and blocked in antibody diluent (PerkinElmer, Waltham, MA). Sections were then sequentially stained for Iba-1 (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... All antibodies were diluted in TNB solution from PerkinElmer. Sections were then incubated for 30 min in streptavidin-horseradish peroxidase that was diluted (1:200 ...
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bioRxiv - Developmental Biology 2020Quote: ... The TSA-Kit secondary antibody system (Perkin Elmer, NEL763E001KT) was utilized with Lef1 immunostaining.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Bound antibodies were detected using horseradish peroxidase-conjugated secondary antibodies and visualized by chemiluminescence using Western Lightning Plus-ECL reagent (Perkin Elmer, United States). Band intensities were analysed using ImageJ (National Institutes of Health ...