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Citations for PerkinElmer :
101 - 150 of 462 citations for PAK4 5 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 5 µCi/ml [3H]-cholesterol (PerkinElmer) was added in the presence of 10% FBS ...
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bioRxiv - Developmental Biology 2021Quote: ... Heat mediated antigen retrieval was used to process sections (Vector, H-3300) and then samples were blocked for 1 hour in blocking buffer at room temperature (PerkinElmer, FP1020). Primary antibodies diluted in blocking buffer were incubated with samples overnight at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were rinsed 3 times for 2 min in PBS-Tx and incubated in tyramide signal amplification (TSA) blocking reagent (PerkinElmer, FP1012) in PBS-Tx (TSA-block ...
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bioRxiv - Molecular Biology 2020Quote: ... A LANCE Ultra TR-FRET kinase assay was used for measurements of enzyme activities that included an-Ulight labeled (Thr-1342) peptide substrate (catalog #TRF0130-M, PerkinElmer) and a Europium-labeled anti-phospho peptide antibody (catalog # TRF0218-M) ...
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bioRxiv - Neuroscience 2022Quote: ... were challenged with synthetic peptides reconstituted in DMEM/BSA medium and luminescence was measured on a MicroBeta LumiJet luminometer (PerkinElmer). Luminescence was measured for 30 s at a wavelength of 469 nm ...
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bioRxiv - Genomics 2020Quote: ... and 1 μCi of [α-32P]-3’-deoxyadenosine 5’-triphosphate (cordycepin 5’-triphosphate, Perkin Elmer Life Sciences) in 1× terminal deoxynucleotidyl transferase buffer (New England Biolabs) ...
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bioRxiv - Biochemistry 2020Quote: ... the sections were washed and blocked with TN with 0.5% (w/v) Blocking Reagent TSA fluorescein system (Perkin Elmer, Waltham, MA, USA) for 30 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... the PFA-fixed sections were blocked and permeabilized in TNBT (0.1 M Tris, pH 7.4, 150 mM NaCl, 0.5% blocking reagent from Perkin Elmer, 0.5% Triton X-100) for 4h at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... Radioactive counts from the media that remain soluble after TCA precipitation (peptide fragments) and pellets from the cells (total non-degraded cell proteins) were measured in a TopCount radioactivity detector (Perkin Elmer). A “protein degradation index” was calculated as the ratio between the counts detected in the soluble media and the counts detected in the pellet cell fraction (82).
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bioRxiv - Cancer Biology 2019Quote: ... Standard Fmoc-peptide synthesis was used to synthesize peptides on the amino-functionalized solid support by custom modified JANUS liquid handling stations (Perkin Elmer). The binding of antibody to each of the synthesized peptides was tested in a pepscan-based ELISA ...
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bioRxiv - Biophysics 2019Quote: AlphaScreen-based peptide recruitments assays were performed using streptavidin coated donor beads and anti-His-tag antibody coated AlphaLisa acceptor beads (Perkin Elmer). The assays were very sensitive to the distance of the N-terminus to the LXXLL NR-box motif and the use of a flexible Ahx linker ...
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bioRxiv - Biochemistry 2021Quote: ... and the level of 32P-transfer to the SAMS peptide was determined using a Tri-Carb 4810TR liquid scintillation counter (Perkin Elmer).
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bioRxiv - Biochemistry 2023Quote: ... 5 μL of 40 μM SFK peptide was added to each well and raw fluorescence units were measured immediately on an Envision fluorometer (Perkin Elmer) with an excitation wavelength of 344 nm and an emission wavelength of 405 nm in real time for the first 15 min at 15 s intervals ...
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bioRxiv - Biochemistry 2024Quote: ... 250 nM purified MBP-Naa80, 200 µM substrate peptide (Figure 1 and Supplemental Table S1, 50 µM 14C-Ac-CoA (Perkin Elmer) were mixed in acetylation buffer (50 mM Tris-HCl pH 8.5 ...
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bioRxiv - Microbiology 2019Quote: ... 5 μL [α-32P] dATP (Perkin Elmer) mixed with 10 μM cold dATP ...
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bioRxiv - Immunology 2019Quote: ... cyanine 3 and cyanine 5 (Perkin Elmer). The ACD 3-plex negative control probe was run in parallel on separate sections in each experiment to assess the background level and set the acquisition parameter ...
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bioRxiv - Molecular Biology 2020Quote: ... TSA Plus Cyanine 5 (NEL763001KT, Perkin Elmer) was used to visualize ...
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bioRxiv - Cell Biology 2022Quote: ... 5 nM [1-14C]oleate (Perkin Elmer), 50 ng/ml BODIPY 665/676 (Life Technologies) ...
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bioRxiv - Physiology 2023Quote: ... 5 mL of Insta-gel Plus (PerkinElmer) was added ...
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bioRxiv - Microbiology 2019Quote: ... primer (5’-AGUUGUUGAUC) was 32P-labeled at the 5′ position upon incubation with [γ-32P] ATP (PerkinElmer, Waltham, MA) and T4 polynucleotide kinase (NEB ...
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bioRxiv - Developmental Biology 2021Quote: ... where reactions were incubated with 150 nM of the gel-filtration purified WT mouse Naa10 or Naa12 in a 30 µl reaction volume containing each 250 µM substrate peptide and radiolabeled [14C]acetyl-CoA (4 mCi/mmol; PerkinElmer Life Sciences) for 12 min (Naa12 ...
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bioRxiv - Cancer Biology 2023Quote: ... The phospho-peptide product was measured by time-resolved fluorescence resonance energy transfer (TR FRET) between the terbium of the antibody and fluorescein of the phospho-peptide using an EnVision plate reader (PerkinElmer, Waltham, MA) equipped to detect the TR-FRET signal (excitation 320 nm ...
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bioRxiv - Bioengineering 2022Quote: ... PEG-maleimide was reacted with 1 mol equivalence of calpain substrate peptide and 1 mol equivalence of L-cysteine-functionalized VivoTag S-750 (PerkinElmer, Boston, MA), followed by quenching with an excess of L-cysteine ...
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bioRxiv - Biochemistry 2020Quote: ... Transport was initiated by dilution of 1.5 μl proteoliposomes in 80 μl external buffer (50 mM NaCl and 50 μM or 5 μM [3H]glutamine (PerkinElmer) in 20 mM Tris pH 7.0) ...
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bioRxiv - Microbiology 2024Quote: ... Glycoconjugate aliquots of 75 µL to 5 mL (3.5-5 mg/mL in 25% glycerol) were injected and passed over a C4 column (either Perkin Elmer Epic C4 column or alternatively Phenomenex Jupiter 10u C4 300Å 250−10 mm 10 micron) ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 µl of anti-Mouse Acceptor beads (PerkinElmer, diluted 1:500 with 1x detection buffer ...
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bioRxiv - Neuroscience 2020Quote: ... TSA® Plus Cyanine 5 (PerkinElmer, 1:1,000) was used as a secondary fluorophore for C1 probes (Gtpbp2 ...
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bioRxiv - Biochemistry 2020Quote: ... and 5 μg/mL streptavidin donor beads (PerkinElmer) was prepared in peptide assay buffer and added to each well ...
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bioRxiv - Genomics 2022Quote: ... and 5 μg/ml streptavidin donor beads (PerkinElmer) was prepared in peptide or Nuc bead buffer ([Peptide ...
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bioRxiv - Biochemistry 2022Quote: ... 5 µM [methyl-3H] S-adenosyl methionine (PerkinElmer), 10 µM substrate RNA/DNA probe ...
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bioRxiv - Cancer Biology 2019Quote: ... 5 µl of anti-Mouse Acceptor beads (PerkinElmer, diluted 1:500 with 1x detection buffer ...
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bioRxiv - Genomics 2019Quote: ... and loaded with 5 µCi/ml Na251CrO4 (PerkinElmer) overnight ...
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bioRxiv - Cell Biology 2020Quote: LH levels in the medium were measured by means of a time-resolved fluorometric immunoassay with rat LH assay kits (National Hormone and Peptide Program, NHPP) and the Delfia system (Perkin Elmer, Waltham, MA) as reported previously (Kawaminami et al. ...
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bioRxiv - Neuroscience 2023Quote: ... Reactions were initiated by adding 2.5 µM ATP at 27°C and were terminated after 60 min with 10 µL of 2.5X Stop Solution/Detection Mix containing 20mM EDTA and 4nM LANCE Ultra Europium-anti-phospho-PKC (Ala25Ser) peptide antibody (Perkin Elmer, Waltham, MA). After incubation at 27°C for another 60 min ...
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bioRxiv - Immunology 2021Quote: ... was added at 5 μg/ml and incubated for 5 min before imaging using an IVIS Spectrum imaging system (Perkin Elmer). Data were analyzed with Living Image Software 4.1 (Perkin Elmer).
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bioRxiv - Cancer Biology 2020Quote: ... The reverse primer R3 (5′-GCGCCGGTAGTTGCCCTTCTC-3′) was labeled at the 5′ end using [γ-32P]-ATP (PerkinElmer Inc., Waltham, MA, USA) and T4 polynucleotide kinase (Takara) ...
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bioRxiv - Cancer Biology 2021Quote: ... or 5 µl of anti-Rabbit Acceptor beads (PerkinElmer, diluted 1:500 with 1x detection buffer ...
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bioRxiv - Systems Biology 2021Quote: ... and 5′-labeled with 32P-γ-ATP (Perkin Elmer) using T4 Polynucleotide kinase (NEB) ...
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bioRxiv - Cancer Biology 2019Quote: ... or 5 µl of anti-Rabbit Acceptor beads (PerkinElmer, diluted 1:500 with 1x detection buffer ...
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bioRxiv - Genetics 2020Quote: ... followed by TSA® Plus Cyanine 5 (#NEL745E001KT, PerkinElmer). For immunohistochemistry study ...
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bioRxiv - Biochemistry 2023Quote: ... and 5 μg/mL streptavidin donor beads (PerkinElmer, 6760002) was prepared in 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... then 5 mL Optiphase ‘HISAFE’ 2 scintillation cocktail (PerkinElmer) were added ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 nM Europium labeled anti-His antibody (Perkin Elmer), 0.5x Streptavidin-xl665 (Cis Bio ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 5 nM [3H]ryanodine (56 Ci/mmol, PerkinElmer, #NET950), 1 mM EGTA and enough CaCl2 to set free [Ca2+] at 100 nM (pCa 7 ...
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bioRxiv - Cell Biology 2024Quote: ... or 1:1000 Cyanine 5 amplification reagent (PerkinElmer, #FP117024UG) in TSA buffer (ACD ...
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bioRxiv - Molecular Biology 2019Quote: ... The 5’ ends were radiolabeled with P32-γ-ATP (PerkinElmer) by T4 polynucleotide kinase (NEB M0201 ...
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bioRxiv - Microbiology 2019Quote: ... 5 mL of a liquid scintialltion cocktail (Ultima Gold, PerkinElmer) were added and the samples analyzed in a scintillation counter (Tri-Carb 2810TS ...
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bioRxiv - Microbiology 2020Quote: ... 5’end-labeled oligo-DNA probes (γ-[32P]-ATP; PerkinElmer) complementary to a sequence in the 16S rRNA ...
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bioRxiv - Microbiology 2019Quote: ... They were 5′-end labeled by [λ-32P] ATP (PerkinElmer) using T4 polynucleotide kinase (Thermo Scientific) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and 5× Epigenetic buffer (AL008) were from PerkinElmer (Waltham, MA). Chicken core histones were obtained from Millipore (Billerica ...