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Citations for PerkinElmer :
101 - 150 of 1402 citations for Mouse Calcitonin Gene Related Peptide Type 1 Receptor CALCRL ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Saturation binding assay was performed to quantify the receptor expression and equilibrium dissociation constant of the radioligand [3H]-NMS (PerkinElmer, specific activity 80 Ci/mmol). Briefly ...
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bioRxiv - Neuroscience 2021Quote: ... Compound plates containing synthetic peptides in DMEM/BSA were placed in a MicroBeta LumiJet luminometer (PerkinElmer). After loading ...
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bioRxiv - Neuroscience 2020Quote: ... Reactions were performed with 50 nM ULight-Glycogen Synthase (Ser641/pSer657) peptide (Perkin Elmer #TRF0131-M), 15 μM ATP ...
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bioRxiv - Cell Biology 2021Quote: ... The amount of peptide remaining bound was measured using scintillation counting in Ultima Gold (Perkin Elmer). Experiments were performed a minimum of three times to generate an overall mean and standard deviation ...
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bioRxiv - Biochemistry 2022Quote: ... The structures of NTV and the substrate peptide were drawn in ChemDraw 20.0 (Perkin Elmer, USA) and optimized to generate 3D conformations using the LigPrep module in Maestro.
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bioRxiv - Biophysics 2023Quote: ... a Gram-positive parental strain from the American Type Culture Collection (ATCC) were purchased from PerkinElmer Inc. ...
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bioRxiv - Microbiology 2022Quote: ... and the cells were lysed using a luciferase reporter gene assay reagent (Britelite, Perkin Elmer). Infectivity (luciferase activity ...
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bioRxiv - Neuroscience 2019Quote: ... The optical density was measured by an ELISA reader (EnSpire, Multimode Plate Reader, PerkinElmer do Brasil, SP, Brasil).
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bioRxiv - Molecular Biology 2023Quote: ... Cellular viability was determined using the ATPlite 1-step kits (PerkinElmer). 50 µl of ATPlite solution was added in 96-well plates for 15 min ...
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bioRxiv - Neuroscience 2020Quote: Phospho-Syk level from WT mouse primary microglia cultures were determined using AlphaLISA SureFire Ultra kit (PerkinElmer, Waltham, MA, USA) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: Quantification of cell types was performed using the Harmony High Content Imaging and Analysis Software (Perkin Elmer). Percentage of cells positive for desired marker versus total number of live cells was calculated ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 μL/well) was captured overnight at 4°C onto high-protein-binding ELISA plates (Spectraplate 96HB, Perkin Elmer). Plates were washed in PBS/Tween (0.05% v/v ...
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bioRxiv - Microbiology 2019Quote: ... anti-rabbit or anti-mouse secondary antibodies conjugated to horseradish peroxidase were used at 1:10,00 dilution (PerkinElmer). Immunoblots were developed using PerkinElmer Western Lightning Plus-ECL and imaged with a GE Healthcare Amersham Imager 600 ...
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bioRxiv - Physiology 2021Quote: ... Plasma insulin concentrations (from tail vein samplings) were determined using an ultrasensitive mouse insulin AlphaLisa detection kit (cat number AL204C) from Perkin Elmer.
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bioRxiv - Molecular Biology 2019Quote: ... PerkinElmer chemagic FFPE DNA kit (chemagic MSM 1; Perkin Elmer, Baesweiler, Germany), and Applied Biosystem’s MagMAX™ FFPE DNA Isolation Kit (Applied Biosystems ...
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bioRxiv - Biochemistry 2021Quote: ... Substrate peptides or nucleosomes were mixed in a solution containing 9µM tritiated S-Adenosyl Methionine (SAM, Perkin Elmer) cofactor ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 μL immunoprecipitated enzyme were mixed with 300 μM synthetic peptide and 50 μM [14C]-acetyl-CoA (PerkinElmer) in acetylation buffer (50 mM HEPES pH 8.5 ...
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bioRxiv - Immunology 2020Quote: ... Cells were then lysed using Britelite plus luminescence reporter gene assay system (Perkin Elmer Catalog no. 6066769) and RLU were measured using the Biotek plate reader ...
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bioRxiv - Cancer Biology 2021Quote: ... firefly luciferase activity was measured using the Britelite Plus Reporter Gene Assay System (Perkin Elmer, Waltham, MA) on a Synergy HT luminometer (BioTeck ...
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bioRxiv - Molecular Biology 2022Quote: ... and luciferase activity was measured with the twinlite Firefly and Renilla Luciferase Reporter Gene Assay System (PerkinElmer). Means of triplicate were calculated for each sample and Firefly/Renilla ratio of stimulated wells was normalized to that obtained in non-stimulated wells.
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bioRxiv - Microbiology 2023Quote: ... cells were lysed using the Britelite plus reporter gene assay system (PerkinElmer, Waltham, MA, USA, Cat# 6066769), and the luminescent signal was measured using a GloMax Explorer Multimode Microplate Reader (Promega).
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bioRxiv - Biophysics 2023Quote: ... Infectivity was measured by using the Steady-lite plus luminescence reporter gene assay system (PerkinElmer, Waltham, MA). Luciferase activity was measured by adding 100 μL of Steady-lite plus buffer (PerkinElmer ...
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bioRxiv - Microbiology 2020Quote: ... MOLT-4/CCR5 cells were added on day 2 and culture supernatants were examined for the p24 viral capsid protein by ELISA (PerkinElmer) after 14 ...
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bioRxiv - Biochemistry 2021Quote: In vitro peptide-based Aur A assays were carried out using a Caliper LapChip EZ Reader platform (Perkin Elmer), which monitors real-time phosphorylation-induced changes in the mobility of a fluorescently labelled Kemptide peptide substrate (5’-FAM-LRRASLG-CONH2 ...
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bioRxiv - Developmental Biology 2022Quote: ... and incubated in 1:200 TSA/amplification buffer (TSA Plus Fluorescence Kits; PerkinElmer) for 30 min to develop signals ...
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bioRxiv - Developmental Biology 2019Quote: ... all samples were incubated in TSA Plus Cyanine5 Evaluation kit (1:50; PerkinElmer) for signal amplification at room temperature for 1 hr with protection from light ...
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bioRxiv - Microbiology 2023Quote: ... filtered through a 0.45-µm-pore size filter and quantified for viral Cap24 content using enzyme-linked immunosorbent assay (ELISA) (Perkin Elmer, # NEK050A) as previously published (41 ...
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bioRxiv - Cell Biology 2020Quote: ... Luciferase activity measurement was performed using the Steadylite plus™ High Sensitivity Luminescence Reporter Gene Assay System (PerkinElmer). β-galactosidase activity measurement was performed using the Galacto-Light Plus system (Applied Biosystems).
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bioRxiv - Cancer Biology 2021Quote: ... HRP-conjugated secondary anti-rabbit and anti-mouse (1:5000) were used and detected by using the enhance Chemiluminescent Substrate (PerkinElmer). The bands were visualized using the ChemiDoc Imaging System (BioRad).
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bioRxiv - Neuroscience 2021Quote: ... Then 5 µl of each diluted cell lysate was mixed with 15 µl of cell lysis buffer containing 0.33 mM ATP and 5 µM ProfilerPro Kinase Peptide Substrate 11 5-FAM-KKLNRTLSVA-COOH (PerkinElmer, U.S.A.) in the presence or absence of 0.66 µM CaM (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... A LANCE Ultra TR-FRET kinase assay was used for measurements of enzyme activities that included an-Ulight labeled (Thr-1342) peptide substrate (catalog #TRF0130-M, PerkinElmer) and a Europium-labeled anti-phospho peptide antibody (catalog # TRF0218-M) ...
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bioRxiv - Biochemistry 2020Quote: ... was incubated with 5 μL of biotinylated peptides at 100 nM for 30 min at room temperature in the epigenetics assay buffer (PerkinElmer AL008 ...
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bioRxiv - Neuroscience 2022Quote: ... were challenged with synthetic peptides reconstituted in DMEM/BSA medium and luminescence was measured on a MicroBeta LumiJet luminometer (PerkinElmer). Luminescence was measured for 30 s at a wavelength of 469 nm ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Binding experiments were performed on membranes prepared from wild-type and HA-MOP-transfected HEK293 cells using [3H]DAMGO (51.7 Ci/mmol) (PerkinElmer NET902250UC) as described previously52 ...
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bioRxiv - Microbiology 2020Quote: ... luciferase activity was measured using the Britelite plus reporter gene assay system and Wallac BetaMax plate reader (Perkin Elmer).
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bioRxiv - Cancer Biology 2023Quote: ... The promoter activity for each cell line was determined using Steadylite Plus reporter Gene Assay System (PerkinElmer Cat # 6066751) according to manufactural manual ...
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bioRxiv - Cancer Biology 2023Quote: ... HRP-coupled anti-mouse-HRP was used (1:2500, Amersham) and detection was done by chemiluminescence (Western Lightning Plus ECL, Perkin Elmer) using the Amersham Imager 600 (GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... Radioactive counts from the media that remain soluble after TCA precipitation (peptide fragments) and pellets from the cells (total non-degraded cell proteins) were measured in a TopCount radioactivity detector (Perkin Elmer). A “protein degradation index” was calculated as the ratio between the counts detected in the soluble media and the counts detected in the pellet cell fraction (82).
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bioRxiv - Cell Biology 2019Quote: ... GST-Aq-NF-κB or GST-Aq-NF-κB-ALA C-terminal peptides and 5 μCi [γ-32P] ATP (Perkin Elmer) in kinase reaction buffer (25 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2019Quote: ... Standard Fmoc-peptide synthesis was used to synthesize peptides on the amino-functionalized solid support by custom modified JANUS liquid handling stations (Perkin Elmer). The binding of antibody to each of the synthesized peptides was tested in a pepscan-based ELISA ...
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bioRxiv - Biophysics 2019Quote: AlphaScreen-based peptide recruitments assays were performed using streptavidin coated donor beads and anti-His-tag antibody coated AlphaLisa acceptor beads (Perkin Elmer). The assays were very sensitive to the distance of the N-terminus to the LXXLL NR-box motif and the use of a flexible Ahx linker ...
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bioRxiv - Biochemistry 2021Quote: ... and the level of 32P-transfer to the SAMS peptide was determined using a Tri-Carb 4810TR liquid scintillation counter (Perkin Elmer).
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bioRxiv - Biochemistry 2023Quote: ... 5 μL of 40 μM SFK peptide was added to each well and raw fluorescence units were measured immediately on an Envision fluorometer (Perkin Elmer) with an excitation wavelength of 344 nm and an emission wavelength of 405 nm in real time for the first 15 min at 15 s intervals ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed twice and cell-associated activity was subsequently measured in a well-type gamma-counter (Wallac 2480 wizard, Perkin Elmer). In a separate binding assay equimolar concentrations (1.97 pM ...
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bioRxiv - Developmental Biology 2022Quote: ... The tyramide signal amplification plus Cy3 Kit (1:1500) (Perkin Elmer Inc: NEL744001KT, Waltham, MA) was then used for detection ...
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bioRxiv - Microbiology 2021Quote: ... 1 μg of total RNA was used for library preparation using NEXTflex v2 kit (PerkinElmer) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... tissue was processed using a TSA kit with Cy3-tyramide (1:50, PerkinElmer, Waltham, MA) for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... Plates were incubated for 48 h and the degree of viral entry was determined by luminescence using the neolite reporter gene assay system (PerkinElmer). IC50 values were determined by fitting dose-response curves with four-parameter logistic regression in GraphPad Prism 8 software.
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bioRxiv - Immunology 2020Quote: ... Plates were incubated for 48 hours and levels of viral infection were determined by luminescence using the neolite reporter gene assay system (PerkinElmer). Virus alone was used as a control and data was normalized to the control.
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bioRxiv - Cell Biology 2024Quote: ... we used the following secondary antibodies: HRP-labeled goat anti-rabbit and mouse IgG (1:10000, NEF812 and NEF822, PerkinElmer, Waltham, MA). For immunofluorescence ...