Labshake search
Citations for PerkinElmer :
101 - 139 of 139 citations for L Asparagine H2O 13C4; D3; 15N2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: ... and medium was exchanged for Leibovitz L-15 CO2-independent medium for imaging at 37°C on a spinning disc confocal system (Ultraview Vox, PerkinElmer) with a 100 × 1.4 NA oil-immersion objective ...
-
bioRxiv - Biochemistry 2021Quote: ... 150 μl of the supernatant was carefully transferred and radioactivity was measured in a liquid scintillation counter (Tri-Carb 3110TR, PerkinElmer). All samples were measured in triplicate ...
-
bioRxiv - Microbiology 2020Quote: ... 200 μCi (7.4 MBq) [35S]-methionine/L-[35S] cysteine using EasyTag™ EXPRESS35S Protein Labeling Mix (PerkinElmer, Groningen, the Netherlands) was added to the medium ...
-
bioRxiv - Cancer Biology 2021Quote: ... cells were cultured in α-MEM containing media containing 2μM Glutamine and 4 mCi/mL L-[3,4-3H(N)]-Glutamine (PerkinElmer, NET551250UC). GLS activity was terminated by washing cells with ice cold HBSS for three times followed by scaping cells with 1 mL ice cold milliQ water ...
-
bioRxiv - Microbiology 2020Quote: ... The dissolution kinetics of L-Ag NPs in mLB was measured by inductively coupled plasma optical emission spectrometry (ICP-OES) (Avio 200, PerkinElmer). All measurements were performed in duplicate ...
-
bioRxiv - Molecular Biology 2021Quote: ... 6 pmole trypSL (1.25 µl from a 50µM stock was kinased with 2 µl 32PgammaATP (25 µM, 6000Ci/mmol, 150 mCi/ml, Perkin Elmer) with 10 U PNK in 10 µl with 20 U RNasin (Roche) ...
-
bioRxiv - Microbiology 2022Quote: ... and at indicated times were washed and incubated for 30 mins in methionine-free DMEM before adding 50μCi of L-[35S]-methionine (Perkin Elmer) for a further 30 min ...
-
bioRxiv - Neuroscience 2022Quote: ... suspended cortical cells from each embryo were seeded in poly-L-lysine-coated 24-well plates (View Plate 96, PerkinElmer) at 250000 cells/well ...
-
bioRxiv - Microbiology 2024Quote: ... Of each sample three replicates and two formaldehyde-fixed (final conc. 1.85 %) adsorption blanks were spiked with [methyl-3H]-thymidine and L-[14C(U)]-leucine (Perkin Elmer) at saturating concentrations of 20 nM and 150 nM ...
-
bioRxiv - Immunology 2024Quote: ... [3H]-thymidine was added at 0.5μl (∼0.5 μCi) per well and plates incubated for 6 h before cells were harvested to UniFilter plates (Perkin Elmer) using a Tomtec 96 Harvester ...
-
bioRxiv - Immunology 2022Quote: ... was added to each well (100 µl/well) and the relative light units (RLU) of luciferase activity was measured using a VICTOR X4 multiplate reader (PerkinElmer).
-
bioRxiv - Systems Biology 2022Quote: ... 100 μl were transferred to a white 96-well microplate and luminescence was measured using on an EnVision plate reader (PerkinElmer).
-
bioRxiv - Genomics 2023Quote: ... at a total concentration equivalent to 0.8 μM nucleosomes and 5.0 μM 14C-SAM (S- [methyl-14C]-adenosyl-l-methionine (PerkinElmer, no. NEC363050UC)) ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... 0.05□μl of 5□mM stock compounds in DMSO were dispensed into assay microplates with a Janus 384 MDT NanoHead (PerkinElmer). Final concentration of the screening compounds in the assay was 16LμM ...
-
bioRxiv - Microbiology 2023Quote: Brine samples BP3 and BP4 were spiked with L-[4,5-3H] leucine (Perkin Elmer, specific activity 100 Ci mmol-1) at a final concentration of 20 nmol leucine L-1 (Kirchman et al. ...
-
bioRxiv - Cell Biology 2024Quote: ... and 20 µL of solution (10,000 cells) was plated into the bottom of 384-well CellCarrier Ultra tissue culture plate (PerkinElmer; 6057300) pre-spotted with compounds (10 µM final concentration) ...
-
bioRxiv - Biochemistry 2020Quote: ... 20 μl of the supernatant was spotted in a scintillation tube and upon the addition of Ultima Gold scintillation liquid (Perkin Elmer) the signal was measured by scintillation counter.
-
bioRxiv - Cell Biology 2021Quote: ... 90 μL of the cell suspension was also plated in duplicate into wells of a 96-well black-walled plate (PerkinElmer OptiPlate), and Venus fluorescence was determined using an excitation wavelength of 485 nm (5 nm bandwidth ...
-
bioRxiv - Molecular Biology 2022Quote: ... 1mM PMSF, 100mM KCl and 1 mCi of [3H]AdoMet (S-adenosyl-L-[methyl-3H] methionine, 80 Ci/mmol; SAM) (Perkin Elmer) or 0.15mM unlabeled S-adenosyl methionine for 45 min at 30°C ...
-
bioRxiv - Molecular Biology 2020Quote: Purified protein complexes were incubated at room temperature with a histone substrate at a final concentration of 500nM and approximately 500nM radioactive SAM (Adenosyl-L-methionine S-methyl3H from PerkinElmer (NET155H250UC)) in methyltransferase buffer (10% glycerol ...
-
bioRxiv - Neuroscience 2020Quote: ... and incubated 10 minutes at 37 °C by addition of 1 μCi/ml of L-[3,4-3H]-Glutamic acid (Perkin Elmer, NET490) and 10µM L-glutamate (Sigma) ...
-
bioRxiv - Microbiology 2020Quote: ... rabbit reticulocyte lysate was incubated with 1μg of the appropriate expression plasmid and 2mL of [35S] L-methionine (1,000 Ci/mMol at 10mCi/mL) (Perkin Elmer, NEG709A) for 90 minutes at room temperature ...
-
bioRxiv - Microbiology 2022Quote: ... The production of LepB–SecM and LepB–CutF variants was induced by the simultaneous addition of 0.1 M IPTG and 2 µl of L-[35S] methionine-cysteine (7 mCi/ ml, PerkinElmer Life Sciences) (38) ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Analytical estimation of the stock CoCl2 solution concentration (100 mmol L−1) was performed employing inductively coupled mass spectrometry (Perkin Elmer Avio 200 optical emission spectrometer ...
-
bioRxiv - Microbiology 2024Quote: ... and the uptake of 14C(U) l-arginine was measured in the bacterial lysates using a liquid scintillation counter (Perkin Elmer).
-
bioRxiv - Microbiology 2024Quote: ... gingivalis wild-type strain was incubated in 5 mL of TSB or TSBNH (a nutritionally limited TSB media that lacks hemin) media with or without radiolabeled L-[14C(U)] Glutamate (1.0 mCi/mmol, Perkin Elmer # NEC290E050UC) at 37°C under anaerobic condition ...
-
bioRxiv - Cancer Biology 2020Quote: ... proteins were first labeled for 48 h with 0.25 μM Ci ml-1 L-[U-14C] VALINE (Perkin Elmer, Waltham, MA, USA) in GIBCO-RPMI 1640 medium (Invitrogen ...
-
bioRxiv - Molecular Biology 2023Quote: ... were measured using a radiometric assay that detects transfer of a [3H] methyl from the radio-labeled cofactor S-[Methyl-3H]-adenosyl-L-methionine (SAM, PerkinElmer, cat. NET155001MC) to a biotinylated peptide Akt1-K64 (QCQLMKTERPRPNTFIIRCLQW ...
-
bioRxiv - Bioengineering 2022Quote: ... PEG-maleimide was reacted with 1 mol equivalence of calpain substrate peptide and 1 mol equivalence of L-cysteine-functionalized VivoTag S-750 (PerkinElmer, Boston, MA), followed by quenching with an excess of L-cysteine ...
-
bioRxiv - Molecular Biology 2023Quote: DNA methylation of replicating sperm or erythrocyte nuclei in egg extract was assayed by the incorporation of 3H-SAM (S-[methyl-3H]-adenosyl-L-methionine; Perkin Elmer, NET155H). Demembranated sperm nuclei were prepared as published previously (86) ...
-
bioRxiv - Cancer Biology 2024Quote: ... solution (5 µg/l) was selected as the internal standard and added online during the ICP-MS analysis (NexION 2000, Perkin Elmer, USA). The method detection limits were also checked periodically ...
-
bioRxiv - Cell Biology 2021Quote: ... sections were washed in TBST and incubated with Alexa Fluor® 594 AffiniPure Goat Anti-Rabbit IgG (H+L) (Abacus DX, Australia) with Spectral DAPI (FP1490) added (1/1000) (Perkin Elmer, USA) for one hour at room temperature ...
-
bioRxiv - Neuroscience 2021Quote: NPCs were seeded onto poly-L-ornithine/laminin-coated 6-well and cyclic olefin co-polymer-based 96-well Cell Carrier Ultra microtiter plates (Perkin Elmer, Waltham, MA) at 1×105 and 2×103 cells/well ...
-
bioRxiv - Bioengineering 2022Quote: Quantification of the metals present in the shell using ICP-OES was performed in the year 2016 with freshwater L fortunei shell only with an inductively coupled atomic emission spectrometer (ICP OES, Perkin Elmer-Optima 3000). In 2022 we again quantified the metals present in shell samples of the L fortunei and in shell samples of marine P perna mussel ...
-
bioRxiv - Developmental Biology 2022Quote: ... Cells were then mechanically detached using 275 μL DPBS and 80 μL of the resuspension was distributed in triplicate to a 96-well black/white isoplate (Perkin Elmer Life Sciences). Next ...
-
bioRxiv - Biochemistry 2024Quote: ... Ninety μl of cells pre-incubated with D-luciferin were added to a white opaque 96-well plate (Opti-Plate, PerkinElmer Life Sciences, 6005290) before measuring luminescence without filters at 28 °C every 10 s in a BMG Labtech POLARStar Omega plate reader ...
-
bioRxiv - Developmental Biology 2020Quote: Recombinant GST-SRF protein or synthetic SRF peptides were incubated for 3 h at 30 °C with GST-SET7 in the presence of 100 nCi of S-adenosyl-[methyl-14C]-L-methionine [14C-SAM] (Perkin Elmer, Waltham, MA, USA) in HMTase assay buffer (50 mM Tris–HCl [pH 8.5] ...
-
bioRxiv - Microbiology 2022Quote: ... and 200 μl of parasites were applied to each well of HFF cells grown in 96-well plates with transparent bottoms (Perkin Elmer cat. no. 6055300). Plates were centrifuged at 290 g for 5 minutes ...
-
bioRxiv - Cancer Biology 2024Quote: ... cells were washed with PBS and incubated in DMEM containing 1 g/L D-glucose and 2 μCi/mL [U-14C]C-glucose (Perkin Elmer, Sydney, NSW, Australia) for 2 h at 37°C ...