Labshake search
Citations for PerkinElmer :
101 - 144 of 144 citations for H Leu arg pro gly nh2 2hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... and 20 μl of the reaction mixture was incubated at 26°C for 1 h in a 384-well AlphaPlate (PerkinElmer). Subsequently ...
-
bioRxiv - Cancer Biology 2021Quote: Quantitation of each individual tumor was measured from H&E sections using morphometric analysis in Panoramic viewer software (Perkin Elmer), which calculates the size of each identified tumor by area in squared microns ...
-
bioRxiv - Biochemistry 2021Quote: ... microsomes prepared from HEK293 cells expressing RyR2 were incubated for 1 h at 25 °C with 5 nM [3H]ryanodine (PerkinElmer) in reaction media containing 0.17 M NaCl ...
-
bioRxiv - Immunology 2020Quote: ... Luciferase activity was measured after 40 h of incubation by addition of an equal volume of substrate NeoLite (Perkin Elmer) followed by luminescence read out on the EnSight Multimode Plate Reader (Perkin Elmer) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Plates were incubated in the dark for 2 h at room temperature before measuring fluorescence using the EnVision Multimode Plate Reader (PerkinElmer), as described previously74 ...
-
bioRxiv - Microbiology 2020Quote: ... which were exposed to X-ray film for 1 h and detected by a storage phosphor system (Cyclone, PerkinElmer, USA).
-
bioRxiv - Cancer Biology 2021Quote: 4T1 cells were cultured for 6 h in RPMI medium containing 0.4 μCi ml−1 [5-3H]D-glucose (Perkin Elmer) after which the supernatant was transferred into glass vials sealed with rubber stoppers ...
-
bioRxiv - Microbiology 2022Quote: ... The plate was then incubated for 1 h at RT in the dark before the AlphaLISA signal was detected using an EnSpire Multimode Plate Reader (PerkinElmer). Negative control with binding buffer or only with one of the complexes were used to control the assay quality ...
-
bioRxiv - Biochemistry 2022Quote: ... Plates were incubated at RT for 1 h and the fluorescence intensity measured at 620 nm and 665 nm by an EnVision multilabel plate reader (PerkinElmer).
-
bioRxiv - Cancer Biology 2023Quote: ... Each protein (5 µL) was mixed and incubated for 1 h in a 384-well AlphaPlate (PerkinElmer, Waltham, MA, USA). After diluting Alpha Screen Protein A Acceptor Beads (PerkinElmer ...
-
bioRxiv - Immunology 2024Quote: ... [3H]-thymidine was added at 0.5μl (∼0.5 μCi) per well and plates incubated for 6 h before cells were harvested to UniFilter plates (Perkin Elmer) using a Tomtec 96 Harvester ...
-
bioRxiv - Cancer Biology 2024Quote: ... The plate was sealed and incubated at RT for 24 h before the AlphaLISA signal was measured using an Envision platereader (PerkinElmer).
-
bioRxiv - Molecular Biology 2024Quote: ... was added to the sample before incubation at 50°C for 5 h and the further addition of 5 ml of liquid scintillation cocktail (Hionic-Flour, PerkinElmer). Leucine uptake in each organ was quantified by measurement of radioactivity with a scintillation counter (LSC-7400 ...
-
bioRxiv - Cell Biology 2024Quote: ... The plate was scanned and images were acquired in real-time every 2 hours from 0 to 24 h post-treatment by Opera Phenix High Content Screening System by PerkinElmer, Inc ...
-
bioRxiv - Developmental Biology 2021Quote: ... Heat mediated antigen retrieval was used to process sections (Vector, H-3300) and then samples were blocked for 1 hour in blocking buffer at room temperature (PerkinElmer, FP1020). Primary antibodies diluted in blocking buffer were incubated with samples overnight at 4°C ...
-
bioRxiv - Microbiology 2021Quote: ... 20 mM HEPES (pH 7.2 were seeded 48 h before treatment at a density of 15,000 cells per well in 96-well imaging plates (PerkinElmer cat. 6005182). Inhibitors ...
-
bioRxiv - Cell Biology 2021Quote: ... was monitored every 5 min for 2 h at 37°C using a fluorescent plate reader (EnSpire Multimode Plate Reader, Perkin Elmer). Samples were assayed in technical triplicates and in biological replicates as indicated in the figure legend.
-
bioRxiv - Cell Biology 2022Quote: ... Tumor size of each individual tumor was measured from H&E stained sections using morphometric analysis in Panoramic viewer software (Perkin Elmer). Tumor burden ...
-
bioRxiv - Molecular Biology 2020Quote: ... cells were incubated at restrictive temperature (37°C) for 3 h and finally transfer to 384-well CellCarrier plates (PerkinElmer, 6007550) for imaging on an Opera QEHS high-content screening microscope (PerkinElmer) ...
-
bioRxiv - Biophysics 2020Quote: ... The undifferentiated cells were seeded to 60% confluency to adhere for 24 h in a CellCarrier-96 Ultra Microplates (Perkin Elmer), which was followed by the media replacement with a fresh media containing FCS reduced to 1% and supplemented with 10 μM trans-Retinoic acid (Abcam ...
-
bioRxiv - Immunology 2024Quote: Bacterial burden in MRSA /ux-infected mouse ears was monitored every 24 h for 3 or 7 consecutive days using an In Vivo Imaging System (IVIS) Spectrum (Perkin Elmer). Bacterial burden was quantified as the bioluminescence (radiance ...
-
bioRxiv - Molecular Biology 2023Quote: ... Fluorescent images were followed 24 and 72 h post-injection with the 3D scanner of the in vivo imaging system (IVIS® 200 Spectrum CT, PerkinElmer) and analyzed by the Living Image Software Version 4.5.2.18424 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Fluorescence emission was monitored at 24 and 72 h using the 3D scanner of the IVIS® Spectrum in vivo imaging system (PerkinElmer) and analyzed by Living Image Version 4.5.2 software after a single administration at 0.5 mg/kg/injection ...
-
bioRxiv - Cancer Biology 2024Quote: ... The fluorescence intensity was measured after 1 h of incubation using an Envision 2104 Multilabel Plate Reader (PerkinElmer, Waltham, MA, USA) with an excitation wavelength of 380 ± 8 nm and an emission wavelength of 430 ± 8 nm.
-
bioRxiv - Cancer Biology 2020Quote: ... proteins were first labeled for 48 h with 0.25 μM Ci ml-1 L-[U-14C] VALINE (Perkin Elmer, Waltham, MA, USA) in GIBCO-RPMI 1640 medium (Invitrogen ...
-
bioRxiv - Cell Biology 2021Quote: RAW cells were incubated for 24 h with inositol-free DMEM (MP Biomedica, CA) containing 10 μCi/ml myo-[2-3H(N)] inositol (Perkin Elmer, MA), 1X insulin-transferrin-selenium-ethanolamine (Gibco) ...
-
bioRxiv - Microbiology 2020Quote: ... Expression of firefly luciferase was determined 24 h after transfection using the Britelite plus Reporter Gene Assay System (PerkinElmer Inc., Milan, Italy) at a Victor X2 multilabel plate reader (PerkinElmer Inc. ...
-
bioRxiv - Immunology 2020Quote: ... and allowed to refold at 18°C for 24 h in a Streptavidin-coated scintillation microplate (Flashplate PLUS, Perkin Elmer, Boston, MA). Dissociation was initiated by adding excess unlabeled β2m and placing the microplate in a scintillation counter (TopCount NXT ...
-
bioRxiv - Microbiology 2021Quote: ... Viral supernatant was then used to transduce each cell line of interest for 48 h and luciferase activity was measured with Promega Steady-Glo® kit (E2550) using a Victor X3 Multilabel Reader (Perkin Elmer). Each experiment shown is representative of three independent experiments.
-
bioRxiv - Immunology 2020Quote: ... The plates were further incubated for 48 h and the extent of virus neutralization was assessed by measuring relative luminescence units in a luminometer (Victor X2, PerkinElmer Life Sciences).
-
bioRxiv - Cancer Biology 2023Quote: ... Intracellular GSH and GSSG levels were determined after 48 h of FR054 and erastin in single or combined treatment by VICTOR X3 multimode plate reader (PerkinElmer, Milan, Italy) using a Total GSH / GSSG colorimetric kit (CO-K097-M ...
-
bioRxiv - Cell Biology 2023Quote: ... 5 μl cAMP Eu-cryptate reagent and 5 μl of anti-cAMP-d2 working solution were added to the 384-well plates and incubated for 1 h.54 Fluorescence signals were detected at 620/665 nm using the Multimode Plate Reader (PerkinElmer EnVision 2105).55 Data were analyzed with GraphPad Prism 9.0 ...
-
bioRxiv - Immunology 2023Quote: ... Fluorescent signals were monitored in real-time with a microplate reader for 24 h within a tissue culture incubator (37 °C, 5 % CO2) on black OptiPlate 96-well (PerkinElmer, MA, USA) under 20x objective ...
-
bioRxiv - Molecular Biology 2024Quote: ... and 2.3±0.64 pmol/mg protein (Bmax for β 2AR) and binding affinities for the radioligand [³H]CGP12,177 (specific activity 51 Ci/mmol, PerkinElmer, Rodgau, Germany) of 0.070 nM (KD for β1AR ...
-
bioRxiv - Cancer Biology 2024Quote: ... The cells were then pulsed for 1 h with 200 μCi/ml of [35S]-Met(methionine)/[35S]-Cys(Cysteine) (Perkin Elmer, NEG072002MC) and chased for 0 ...
-
bioRxiv - Cell Biology 2022Quote: ... Luminescence was measured at 1 h post PathHunter© detection reagent addition using Victor3 plate reader (Perkin Elmer 0.5-s/well integration time). Data were normalized to control treated with the highest concentration of ligand (100%) ...
-
bioRxiv - Cell Biology 2021Quote: ... sections were washed in TBST and incubated with Alexa Fluor® 594 AffiniPure Goat Anti-Rabbit IgG (H+L) (Abacus DX, Australia) with Spectral DAPI (FP1490) added (1/1000) (Perkin Elmer, USA) for one hour at room temperature ...
-
bioRxiv - Immunology 2021Quote: ... was performed repetitively in short-term isoflurane anesthesia over a period of 24 h using the IVIS Spectrum In Vivo Imaging System (PerkinElmer, Waltham, MA, USA). Four days after the first Nb administration ...
-
bioRxiv - Molecular Biology 2020Quote: ... The effects of the compounds were evaluated 24 h later using an Operetta CLS Confocal High Content Imaging System (PerkinElmer, Waltham, Massachusetts, USA) with the following parameters ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... lung tissues harvested from euthanized mice were soaked for 10 min in pre-warmed (37°C) PBS with 2% FBS and a 1:10 dilution of Xenolight RediJect Coelenterazine h (Cat# 706506, Perkin Elmer, Waltham, MA). Following a 12-15 min incubation ...
-
bioRxiv - Immunology 2024Quote: ... Luciferase activity was quantified after 48–72 h by the addition of Steadylite Plus chemiluminescence substrate and analysis on a Perkin Elmer EnSight multimode plate reader (Perkin Elmer, Beaconsfield, UK). Antibody titre was then estimated by interpolating the point at which infectivity had been reduced to 50% of the value for the ‘no serum’ control samples.
-
bioRxiv - Cancer Biology 2023Quote: ... and the plate was incubated in the dark for 2 h and then read in an EnSpire Alpha multimode plate reader (PerkinElmer, Waltham, MA, USA).
-
bioRxiv - Biochemistry 2021Quote: ... Plates were incubated at room temperature for 2 h before measurement of the fluorescence using an EnVision Multimode Plate (Reader PerkinElmer Life and Analytical Sciences). All values were converted to cAMP concentration using cAMP standard curve performed parallel and data were subsequently normalized to the response of 100 μM forskolin in each cell line ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were imaged at 1 frame/30 s for 4 h at 37°C with 5% CO2 using a spinning disk confocal microscope (UltraVIEW VoX, PerkinElmer with a 63X 1.4 N.A. oil objective), followed by addition of 50 μg/ml PI (Thermo Fisher Scientific ...