Labshake search
Citations for PerkinElmer :
101 - 150 of 224 citations for Dig 6C SAH 1a b c since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2023Quote: ... Relative luminescence units (RLU) were detected on a pre-warmed 37°C EnVision plate reader (Perkin Elmer, Waltham, MA) for 30 min to reach the maximum value ...
-
bioRxiv - Genomics 2024Quote: ... and the run-on conducted in the presence of 25 μM biotin-11-A/C/G/UTP (Perkin Elmer cat nrs ...
-
bioRxiv - Cell Biology 2020Quote: ... and medium was exchanged for Leibovitz L-15 CO2-independent medium for imaging at 37°C on a spinning disc confocal system (Ultraview Vox, PerkinElmer) with a 100 × 1.4 NA oil-immersion objective ...
-
bioRxiv - Microbiology 2020Quote: ... the plates containing infected macrophages were incubated at 37°C with 5% CO2 for 18 h in an Operetta High-Content Imaging System (PerkinElmer). During this 18 h incubation ...
-
bioRxiv - Developmental Biology 2021Quote: ... the cells were incubated for 5 minutes at 37°C with 55µCi/well of 35S-L-methionine and 35S-L-cysteine Promix (Perkin Elmer) in a minimal volume of culture medium ...
-
bioRxiv - Cell Biology 2022Quote: Images of embryos were acquired at 23°C using a PerkinElmer ERS spinning disk confocal system mounted on a Zeiss Axiovet 200M microscope using Volocity software (PerkinElmer). A 63X ...
-
bioRxiv - Biophysics 2022Quote: ... incubated for 4 h at 37°C and fluorescent signal intensity was quantified using the EnSpire Multimode Plate Reader (PerkinElmer) at excitation and emission wavelengths of 540 and 612 nm respectively.
-
bioRxiv - Cell Biology 2022Quote: ... cell motility was acquired using temperature (37°C) and CO2 (5%) controlled Operetta CLS High-Content Analysis System (PerkinElmer, HH16000000) (non-confocal ...
-
bioRxiv - Biochemistry 2021Quote: ... Fluorescence intensity (Ex = 485 nm/Em = 535 nm) was monitored at 37 °C with a microplate reader VictorIII (Perkin Elmer). A calibration curve was created by measuring multiple concentrations (from 0.001 to 5 μM ...
-
bioRxiv - Biochemistry 2021Quote: ... The samples were boiled at 95°C for 10 min and subjected directly to LabChip GX II capillary gel electrophoresis (PerkinElmer) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2020Quote: ... TR-FRET readout was performed after 1 h incubation at 4°C using an EnVision 2105 Multimode Plate Reader (PerkinElmer) with previously defined measurement parameters53 ...
-
bioRxiv - Cancer Biology 2020Quote: ... Live-cell imaging was performed in a humidified environment at 37°C using a spinning-disc confocal microscopy system (UltraVIEW Vox; PerkinElmer) and a charge-coupled device camera (ORCA-R2 ...
-
bioRxiv - Cancer Biology 2021Quote: Bioluminescence signals in exosomes and MVs (3 × 107 EVs/µL) carrying PalmReNL were measured at room temperature (25°C) using a VICTOR Nivo Microplate Reader (PerkinElmer). EVs were incubated in the presence or absence of 1% Triton X-100 for 30 min at 37°C ...
-
bioRxiv - Molecular Biology 2020Quote: ... with the confocal setting at 40X magnification at 37°C and 5% CO2 and analyzed with Columbus Image Analysis System (PerkinElmer). Each well was excited with 425 nm light and emissions detected at 434-515 nm for CFP and 500-550 nm for YFP ...
-
bioRxiv - Neuroscience 2022Quote: ... The samples were incubated for 30 min at 37 °C in the presence of the EasyTag Express Protein Labeling Mix 35S (PerkinElmer), pelleted ...
-
bioRxiv - Neuroscience 2022Quote: ... To quantify α1-3;5GABAAR79 sections were incubated for 60 minutes at 4°C in 1nM [3H]flumazenil (Perkin Elmer, NET757001MC), or in 1nM [3H]flumazenil with 10 µM flunitrazepam (Sigma Aldrich ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... and 20 μl of the reaction mixture was incubated at 26°C for 1 h in a 384-well AlphaPlate (PerkinElmer). Subsequently ...
-
bioRxiv - Biochemistry 2021Quote: ... microsomes prepared from HEK293 cells expressing RyR2 were incubated for 1 h at 25 °C with 5 nM [3H]ryanodine (PerkinElmer) in reaction media containing 0.17 M NaCl ...
-
bioRxiv - Cell Biology 2020Quote: Embryos and third instar larval larval brains were imaged at 21°C on a Perkin Elmer ERS spinning disk (Volocity software version 6.3, PerkinElmer Inc.) mounted on a Zeiss Axiovert 200M microscope using a 63X/1.4-NA oil immersion objective and an Orca ER CCD camera (Hamamatsu Photonics) ...
-
Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. colibioRxiv - Microbiology 2020Quote: ... Reactions were incubated at 25 °C for 10mins followed by addition of 5 μCi of [γ-32P] ATP(Perkin Elmer). Reactions were incubated for additional 30 mins at 37 °C and were stopped using 3X Laemmli buffer ...
-
bioRxiv - Molecular Biology 2022Quote: ... was next added and incubated with the mixture at 4 °C for 1 hour before the measurements performed on Envision Readers (PerkinElmer). The fluorescently-labeled m6A-containing mRNA was used to adjust the gain factor ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were washed with PBS and then stored in PBS at 4°C until imaged in the Opera Phenix (PerkinElmer) at 40x magnification and images analysed using a Harmony software to assess protein expression and morphology (see supplementary).
-
bioRxiv - Bioengineering 2023Quote: ... of approximately 5.7 ± 3.0 pmol/mg protein measured by the manufacturer using [3H]-DAMGO (ES-542-C OP3 ValiScreen, PerkinElmer, Waltham, MA) were obtained and cultured for a series of binding assays ...
-
bioRxiv - Molecular Biology 2022Quote: ... RNA samples were separated on a 7 M urea denaturing polyacrylamide gel at 4°C at 100V and transferred onto a charged nylon transfer membrane (PerkinElmer), followed by cross-linking using a UV Stratalinker and drying at 80°C on a Gel Dryer (Bio-Rad).
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Plates were incubated in the dark for 1 hr at 37°C followed by measurement of fluorescence using an Envision plate reader (PerkinElmer) with standard AlphaScreenTM settings ...
-
bioRxiv - Cell Biology 2022Quote: ... The plate was sealed with parafilm and placed in a pre-heated (37°C) Opera Phenix microscope with 40x or 60x water-immersion lens (PerkinElmer) with 5% CO2.
-
bioRxiv - Biochemistry 2024Quote: ... were transferred to 1.5 mL Eppendorf tubes containing 10 μL of pre-incubated at 37°C respective radioisotope (3H methionine [0.77 μCi, Perkin Elmer], 3H uridine [0.1 μCi, Perkin Elmer] or 3H thymidine [0.32 μCi ...
-
bioRxiv - Biochemistry 2024Quote: ... were transferred to 1.5 mL Eppendorf tubes containing 10 μL of pre-incubated at 37°C respective radioisotope (3H methionine [0.77 μCi, Perkin Elmer] ...
-
bioRxiv - Cell Biology 2024Quote: Images of living embryos were acquired at 23°C using a PerkinElmer ERS spinning disk confocal system mounted on a Zeiss Axiovet 200M microscope using Volocity software (PerkinElmer). A 63X ...
-
bioRxiv - Molecular Biology 2024Quote: ... membranes were blocked with 5% BSA in Tris / HCl-buffered saline / 0.05% Tween (TBST) for 24 h at 4 °C and afterwards membranes were incubated for 1 h with HRP-Streptavidin (PerkinElmer; #NEL750001EA ...
-
bioRxiv - Cancer Biology 2024Quote: ... was added and cells were incubated for 90 minutes at 37°C before measuring fluorescence using an EnVision plate reader (PerkinElmer). For longer-term proliferation assays ...
-
bioRxiv - Microbiology 2023Quote: ... Plates were read once every 30 s for 10 min at 37°C using a EnSpire Multimode Plate Reader (PerkinElmer) with excitation and emission wavelengths of 550 and 591 nm ...
-
bioRxiv - Microbiology 2023Quote: ... purified 6×His-tagged SfTIR-STING was added to the reaction to a final assay volume of 50 μL and plates were read once every 15 s for 10 min at 37°C using a EnSpire Multimode Plate Reader (PerkinElmer) with excitation and emission wavelengths of 410 and 300 nm ...
-
bioRxiv - Biochemistry 2023Quote: ... Cells were labeled for 15 min at 37 °C with 100 µCi of 35S-methionine (PerkinElmer Life Sciences, Boston, MA). After incubation ...
-
bioRxiv - Microbiology 2022Quote: ... maintained at 200 °C and with N2 as the gas vector (flow rate of 6 mL·min-1) with a flame ionization detector (FID) maintained at 280 °C (PerkinElmer, USA)
-
bioRxiv - Bioengineering 2022Quote: ... The volatiles were desorbed again by heating (200°C) in an Automatic Thermal Desorber (ATD-400, Perkin Elmer, Norwalk, CN), cryofocused on a cold trap (-30 °C) ...
-
bioRxiv - Biochemistry 2022Quote: ... Steady-state binding signal was obtained after incubation of reactions for 1 hr at 25° C using a EnSight Multimode plate reader (PerkinElmer) with a 30 ms excitation at 680 nm and emission measured at 615 nm after 140 ms ...
-
bioRxiv - Genomics 2022Quote: ... nuclei were extracted from ~2×107 cells and run-on reactions were carried on at 37°C for 3 minutes using 3μl of each 11-biotin-labelled ribonucleotide stocks (Perkin Elmer). Following real time PCR amplification in the presence of EvaGreen dye as above ...
-
bioRxiv - Biochemistry 2023Quote: cKO cells grown in the presence or absence of acetamide normalized to OD600 = 1 were incubated for 2 hours at 37 °C in 7H9 media containing [14C]-acetate (final concentration 0.5 μCi/ml; Perkin Elmer). Labeled cells were harvested by centrifugation at 5,000 x g for 3 minutes ...
-
bioRxiv - Immunology 2023Quote: ... After 10min of incubation at 37°C supernatants were collected and concentration of ATP/sample was measured using ATPlite Luminescence Assay System (PerkinElmer) according to the manufacturer’s protocol.
-
bioRxiv - Genetics 2023Quote: ... Live imaging was performed with a Nikon TE2000 microscope at 37°C using Volocity software (Nikon Corporation, Tokyo, Japan; PerkinElmer). Images were captured with 488 nm excitation and 516 nm emission at a rate of 20 images per second for 30 seconds ...
-
bioRxiv - Neuroscience 2023Quote: ... Live cell imaging was performed at 37°C in 5% CO2 for 24 hours using the Opera Phenix High Content Screening System (PerkinElmer). Acquired images were analyzed by using the Harmony software (PerkinElmer).
-
bioRxiv - Molecular Biology 2024Quote: ... was added to the sample before incubation at 50°C for 5 h and the further addition of 5 ml of liquid scintillation cocktail (Hionic-Flour, PerkinElmer). Leucine uptake in each organ was quantified by measurement of radioactivity with a scintillation counter (LSC-7400 ...
-
bioRxiv - Microbiology 2020Quote: ... Further 160μl of DNS solution was added in each well and incubated at 100°C for 20 min followed by reading at 570nm in multimode plate reader (Perkin Elmer). All the quantitative assays were done in triplicates and the activity was estimated using the standard graph of glucose.
-
bioRxiv - Immunology 2020Quote: ... Plates were stored in the dark at 4°C prior to imaging using a 40x objective mounted on an Operetta High-Content Imaging System (Perkin Elmer) controlled by Harmony 4.1 software.
-
bioRxiv - Biochemistry 2021Quote: ... Complexes were incubated for 1h at 37°C and luminescence released by the [14C]-RITA-excited beads was measured in standard microplate reader (Perkin Elmer).
-
bioRxiv - Cell Biology 2021Quote: ... was monitored every 5 min for 2 h at 37°C using a fluorescent plate reader (EnSpire Multimode Plate Reader, Perkin Elmer). Samples were assayed in technical triplicates and in biological replicates as indicated in the figure legend.
-
bioRxiv - Biochemistry 2021Quote: ... and mRNA was translated for 1 hour at 30 °C in presence of 10 µCi/µL EasytagTM 35S Express Protein Labeling Mix (Perkin Elmer). The mixtures were centrifuged for 2 min at 10,000 x g at 4 °C after inhibiting protein synthesis with 1 mM cycloheximide and translocated CFTR was retrieved from the pellet fraction ...
-
bioRxiv - Cell Biology 2020Quote: ... and recorded on a 37°C heated stage of Nikon Ti inverted microscope using Ultraview spinning disk confocal system (Perkin Elmer). For the TEM opening ...
-
bioRxiv - Developmental Biology 2022Quote: ... The sections were prehybridized and hybridized at 45°C for 4 hours in 50% (Vol/Vol) formamide hybridization buffer containing 35S-labeled antisense RNA probes (Perkin Elmer). RNase A-resistant hybrids were detected by autoradiography ...