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Citations for PerkinElmer :
101 - 150 of 427 citations for 2 4 Dimethyl 4' 4 methylpiperazinomethyl benzophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... since this was when significant peripheral neuropathy first became detectable11.Tumor growth was monitored weekly for up to 4 weeks by bioluminescent imaging using an IVIS Lumina XR (PerkinElmer), as described previously11 ...
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bioRxiv - Immunology 2020Quote: ... Plates were stored in the dark at 4°C prior to imaging using a 40x objective mounted on an Operetta High-Content Imaging System (Perkin Elmer) controlled by Harmony 4.1 software.
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bioRxiv - Cell Biology 2021Quote: ... diluted in buffer A to a final concentration of 4 nM) was added to a 384-well microplate (OptiPlateTM-384 Plus, Perkin Elmer) followed by the addition of 15 μL diluted proteins and 5 μL of the GFP antibody-conjugated acceptor beads (5x) ...
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bioRxiv - Biophysics 2020Quote: ... 100 nM hNatB was mixed with a saturating concentration of “MDVF” peptide substrate (500 μM) and varying concentrations (1.95 μM to 1 mM) of acetyl-CoA (14C-labeled, 4 mCi mmol−1; PerkinElmer Life Sciences) for 10-minute reactions ...
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bioRxiv - Developmental Biology 2022Quote: ... The sections were prehybridized and hybridized at 45°C for 4 hours in 50% (Vol/Vol) formamide hybridization buffer containing 35S-labeled antisense RNA probes (Perkin Elmer). RNase A-resistant hybrids were detected by autoradiography ...
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bioRxiv - Plant Biology 2022Quote: ... 5-mm root segments away from the apical 2 mm root tip and 2 mm away from the root-shoot junction were carefully cut and soaked overnight in 4 ml of liquid scintillation fluid (Ultima Gold, PerkinElmer, USA), and the radioactivity was measured with a scintillation counter (model LS6500 ...
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bioRxiv - Physiology 2020Quote: ... Each filter was then placed in a Sterilin™ scintillation vial and radioactivity determined by: addition of 4 mL of Ultima Gold XR liquid scintillant (PerkinElmer), overnight incubation at room temperature and the retained radioactivity determined using a Beckman Coulter LS 6500 Multi-purpose scintillation counter (Beckman Coulter Inc. ...
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bioRxiv - Neuroscience 2023Quote: ... of incubation in an isotope solution containing rubidium (86Rb+) (1 μCi/ml, 022-105721-00321-0001, POLATOM) and 3H-mannitol (4 μCi/ml, NET101, Perkin Elmer). 86Rb+ acts as a K+ congener ...
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bioRxiv - Neuroscience 2023Quote: ... Thawed serum samples were diluted with 1xPBS 1:4 and assayed in triplicates using a fluorometer (PerkinElmer, VICTOR3V 1420 Multilabel counter) with excitation/emission of 485 nm / 535 nm (25 nm band-pass filter) ...
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bioRxiv - Cancer Biology 2022Quote: ... tissues were cut as 4 μm sections and automated IF was performed on a Leica Bond Rx Platform at room temperature using the PerkinElmer Opal 4-Colour Automation IHC Kit (PerkinElmer, #NEL800001KT). Tissue sections were blocked with 3% Hydrogen peroxide (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... Fluorescently labeled RNA was obtained by replacing 1/4 volume of CTP with Cyanine 3-CTP (Cy3-CTP) or Cyanine 5-CTP (Cy5-CTP) (PerkinElmer NEL581001EA) in the in vitro transcription reaction.
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bioRxiv - Cancer Biology 2024Quote: ... 150 μL 14C-labelled lipids from the denser organic fraction were combined with 4 mL OptiPhase HiSafe 3 liquid scintillation cocktail (PerkinElmer; 1200.437) and radio-labeling was quantified using a Tri-Carb 2910 TR Liquid Scintillation Analyzer (PerkinElmer) ...
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bioRxiv - Molecular Biology 2023Quote: ... Transcription was initiated with addition of 12 pmol rrnBP1 PCR promoter fragment pre-mixed with 1 mM ATP and 4 μCi α-[32P]-CTP (3,000 Ci mmol−1; Perkin Elmer) at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... at 42 °C for 30 min followed by hybridization overnight at 42°C with 4 pmol probes (Table S3) that were radiolabeled using [γ-32P] ATP (PerkinElmer) and T4 Polynucleotide kinase (New England Biolabs) ...
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bioRxiv - Cancer Biology 2023Quote: ... Following an HRP conjugation step a TSA Opal 570 1:150 (Tyramide Signal Amplification Opal 4-Color Manual IHC Kit, Perkin Elmer) was linked to the IRF7 antibody previously bound to the slide overnight ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cells were incubated for four hours at 4 °C in the presence of benzazepine-red nonpeptide vasopressin antagonist (BZ-DY647, PerkinElmer Revvity) at various concentrations ranging from 10-10 to 10-7 M ...
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bioRxiv - Molecular Biology 2024Quote: ... and were then anesthetized (isoflurane 4 % for induction and 1.5 % for maintenance) before placing in the optical imaging system (IVIS Lumina III, PerkinElmer, Courtaboeuf, France). In vivo bioluminescence signals were measured in selected regions of interest (injected testes ...
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bioRxiv - Developmental Biology 2021Quote: ... where reactions were incubated with 150 nM of the gel-filtration purified WT mouse Naa10 or Naa12 in a 30 µl reaction volume containing each 250 µM substrate peptide and radiolabeled [14C]acetyl-CoA (4 mCi/mmol; PerkinElmer Life Sciences) for 12 min (Naa12 ...
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bioRxiv - Microbiology 2022Quote: ... were performed in a HORIBA JOBIN YVON Spectrofluorometer (FluoroMax – 4) (HORIBA Scientific, Edison, New Jersey, USA) in a fluorescence cuvette of path length 5 mm (Perkin Elmer B0631123), after putting 0.5ml of the sample solution in it ...
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bioRxiv - Biophysics 2021Quote: ... 50 nM SpNatC (WT or mutants) was mixed with 50 μM Ac-CoA (14C-labeled, 4 mCi mmol-1; PerkinElmer Life Sciences) and varying peptide concentrations (ranging from 1.95 μM to 500 μM ...
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bioRxiv - Bioengineering 2022Quote: ... Animals were kept under anesthesia (3-4%) following CHP injection and an initial image acquisition was performed using an IVIS Spectrum imaging system (PerkinElmer, Waltham, MA) <15 minutes following injection ...
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bioRxiv - Neuroscience 2023Quote: ... Half of the cell Triton X lysate from each well was pipetted into a scintillation vial and 4 ml of scintillation fluid (#6013329, Ultima GoldTM, Perkin Elmer, USA) was added ...
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bioRxiv - Molecular Biology 2023Quote: ... were prepared in in vitro transcription reaction on linear PCR-generated template containing RNAImod (4) with 500 μM ATP or NAD and 25 μM [α 32P] CTP (Perkin Elmer) using hexa-histidine tagged E ...
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bioRxiv - Microbiology 2024Quote: ... Mixtures were incubated for 60 min at room temperature prior to adding 4 µl of D-luciferin (30 mg/ml) (Perkin Elmer; #122799). After additional incubation for 5 min ...
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bioRxiv - Physiology 2020Quote: ... Progesterone was extracted with ethyl ether after overnight equilibration (4°C) of the plasma with 1500 dpm of tritiated progesterone (Perkin Elmer, Rodgau, Ermany). Separation of the organic and aqueous phase was conducted similar to the procedure described for the other hormones ...
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bioRxiv - Biochemistry 2021Quote: ... coli and all constructs were adjusted to approximately 4 μM concentration in cell lysate soluble fractions using a microcapillary electrophoretic system (Caliper; PerkinElmer, Waltham, MA, USA) and frozen in 96-well plates.
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bioRxiv - Neuroscience 2021Quote: ... and loaded overnight with MEM +HIHS cell culture media supplemented with 4 μCi/mL D-[2,3-3H]aspartate (Perkin Elmer, Waltham, MA, cat. #NET501001MC). Coverslips were washed in isosmotic Basal medium containing (in mM ...
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bioRxiv - Biochemistry 2021Quote: ... an optimized set of 4 small interfering RNAs (siRNAs; SMARTPool) targeted against human Furin were purchased from Horizon Discoveries (Perkin Elmer, Lafayette, LA, USA) and transfections of HeLa cells were carried out using INTERFERin (PolyPlus ...
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bioRxiv - Cancer Biology 2021Quote: ... For multiplexed immunohistochemistry signals were detected using Opal™ 4-Plex Kit according to the manufacturer’s protocol (catalog no. NEL810001KT; Perkin Elmer, Waltham, MA, USA). For more details see Supplemental Materials section.
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated with a D1R antagonist for 1h at 4°C in a solution equivalent to the pre-incubation buffer with the addition of 1nM [3H]SCH23390 (Perkin Elmer, USA, 85 Ci/mmol). Non-specific binding was determined by incubating sections under the same conditions with excess amount of the D1R ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated with a D2R antagonist for 1h at 4°C in a solution equivalent to the pre-incubation buffer with the addition of 4nM [3H]raclopride (Perkin Elmer, USA, 60.1 Ci/mmol). Non-specific binding was determined by incubating sections under the same conditions with excess amount of the D2R ...
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bioRxiv - Neuroscience 2023Quote: ... The experiments were initiated by placing the choroid plexus in HEPES-aCSF containing 1 μCi/ml 86Rb+ (022-105721-00321-0001, POLATOM, as a tracer for K+ transport) and 4 μCi/ml 3H-mannitol (Perkin Elmer, as an extracellular marker [26]) at 37°C for 2 min in the absence or presence of 2 mM ouabain (O3125 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were imaged at 1 frame/30 s for 4 h at 37°C with 5% CO2 using a spinning disk confocal microscope (UltraVIEW VoX, PerkinElmer with a 63X 1.4 N.A. oil objective), followed by addition of 50 μg/ml PI (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... WIN55212-2 (PerkinElmer) (10 µM ...
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bioRxiv - Cancer Biology 2020Quote: ... 2’-14C]-cystine (PerkinElmer) for 5 minutes at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ERK1/2 phosphorylation was detected with the AlphaScreen SureFire ERK1/2 assay kit (PerkinElmer). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... was added to control wells before addition of 2-[3H]deoxyglucose (2-DG) (PerkinElmer). During the final 5 min 2-DG (0.25 μCi ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 mM DTT and 2 μCi/mL S-adenosyl-L-[methyl-14C] methionine (PerkinElmer). After 3h incubation ...
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bioRxiv - Cell Biology 2024Quote: ... a glucose uptake tracer (14 C-2-deoxyglucose (2-DG) (Perkin Elmer, cat # NEC495001MC) was administered as a single bolus at a dose of 13 μCi over 1 minute at 75 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... A Wizard 2 (Perkin Elmer) gamma counter was used to quantify tracer uptake in organs ...
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bioRxiv - Cell Biology 2021Quote: ... 2’-14C]-Cystine (PerkinElmer, NEC854010UC) and washed three times in ice-cold PBS containing cold cystine (1 mM) ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μCi of 2-Deoxy-D-[1-2-3H] glucose (PerkinElmer, Boston, MA, USA # NET328A001MC) and 10μM non-radioactive 2-DG (Sigma Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mL of each component was added to 2 mL Ultima Gold scintillation fluid (PerkinElmer), and radioactivity counted on the MicroBeta2 scintillation counter (PerkinElmer) ...
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bioRxiv - Immunology 2020Quote: ... 2 × 106 treated cells were incubated with 2 MBq of Na2 CrO4 (#NEZ030001MC, PerkinElmer, Waltham, MA) for 2 hours at 37 °C in a 5% CO2 atmosphere and were used as target cells ...
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bioRxiv - Neuroscience 2024Quote: ... 2×2 binning) were aquired using Operetta CLS high content imaging system (PerkinElmer; Waltham, MA, USA) using a 20x air objective and Hoechst and Alexa555 predefined filter sets ...
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bioRxiv - Molecular Biology 2020Quote: ... ~2 nM [α-32P]-ATP (PerkinElmer) and an indicated content of ATP in 50 mM Tris-Cl (pH 6.8 ...
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bioRxiv - Biochemistry 2021Quote: ... myo-[2-3H]inositol (NET114A001MC, PerkinElmer), UDP-[6-3H]GlcNAc (ART 1136 ...
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bioRxiv - Neuroscience 2021Quote: Autoradiographic procedure for the analysis of 3H-2-deoxyglucose (2-DG; 1.5μCi/g body weight, #NET549A001MC, PerkinElmer) localization was adapted from the method by Sokoloff et al ...
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bioRxiv - Biochemistry 2020Quote: ... pH 7.4) with 10 μM 2-deoxy-Glucose and 0.5 μCi/mL 3H-2-deoxy-glucose (Perkin Elmer). Cytocholasin B (5 μM ...
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bioRxiv - Cell Biology 2020Quote: ... λ-32P-ATP (2 μCi; Perkin Elmer) was included in reactions to enable measurement of phosphorylation during assay optimization using a phosphorimager (Typhoon FLA7000 ...