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Citations for PerkinElmer :
1401 - 1450 of 1522 citations for 5 Pyrimidinecarbonitrile 1 2R 2 3 dihydroxypropyl 1 2 3 4 tetrahydro 3 methyl 2 4 dioxo 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... The cells were imaged at 37 °C and 5% CO2 on Operetta CLS high-content imaging system (PerkinElmer) by using DAPI ...
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bioRxiv - Cell Biology 2022Quote: ... 5 µl U2OS medium were added to each well of a 384-well plate (PerkinElmer CellCarrier-384 Ultra). Subsequently ...
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bioRxiv - Cell Biology 2023Quote: ... 5 µL U2OS medium were added to each well of a 384-well plate (PerkinElmer CellCarrier-384 Ultra). Subsequently ...
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bioRxiv - Bioengineering 2023Quote: ... and counted immediately three times for 5 minutes each on a Tri-Carb 2900TR Liquid Scintillation Counter (PerkinElmer). The average DPM from the three technical replicate readings was utilized as the readout ...
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bioRxiv - Cancer Biology 2023Quote: ... mice were injected intraperitoneally with 15 mg/mL (at 5 µL/g BW) of D-Luciferin (Perkin Elmer) and imaged ...
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bioRxiv - Biophysics 2019Quote: ... The solubilized virus was diluted in HB buffer with 1% Triton-X and the fluorescence emission spectrum of the sample was measured using a fluorimeter (Perkin Elmer LS 55, Waltham, MA). The integrated fluorescence intensity across the spectrum was calculated and compared to a standard curve of known dye concentration in the same buffer to determine the concentration of fluorescent dye in the viral suspension ...
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bioRxiv - Microbiology 2021Quote: ... The level of HIV-1 replication was determined by measuring p24 antigen in the culture supernatant using a kit (Perkin Elmer Inc, Shelton, CT, USA).
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bioRxiv - Developmental Biology 2022Quote: ... anti-mouse Chd7 primary antibody was followed by signal amplification with goat anti-rabbit IgG horseradish peroxidase (1: 500) (Perkin Elmer Inc: NEF812001EA, Waltham, MA). The tyramide signal amplification plus Cy3 Kit (1:1500 ...
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bioRxiv - Molecular Biology 2019Quote: ... Detection was carried out by incubating with a 1:5000 dilution of a goat anti-rabbit conjugated to peroxidase (Perkin Elmer and Sigma, Aldrich respectively). The latter were developed with the ECL Plus™ Western blotting detection system (PerkinElmer Life Sciences).
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bioRxiv - Cancer Biology 2022Quote: Optimized numbers of cells (2.5 x 103 to 5 x 103) were plated onto 96-well plates (Perkin Elmer) and allowed to adhere ...
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bioRxiv - Microbiology 2022Quote: ... the dried gel was exposed on phosphoscreen for 5 days and revealed using a Cyclone Plus Phosphor Imager (PerkinElmer).
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bioRxiv - Immunology 2022Quote: 50,000 cells per well were seeded and allowed to attach before gentle washing and incubation for 48 hours in media containing 0.4 µCi/mL (24.24 nM final concentration) [5-3H]glucose (Perkin Elmer) or 2 µCi/mL (66.67 nM final concentration ...
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bioRxiv - Microbiology 2022Quote: ... The membranes were incubated with primary and secondary HRP-conjugated antibodies in PBST (5% milk and 0.1% Tween-20) to detect the protein of interest by Western Lightning Plus ECL (PerkinElmer) and ImageQuant LAS 4000 imaging system (GE) ...
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bioRxiv - Microbiology 2022Quote: ... A series of images spanning 5 µm were acquired with 0.2 µm spacing and images were deconvolved with VOLOCITY software (PerkinElmer) to report a single combined z-stack image.
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bioRxiv - Cancer Biology 2022Quote: ... the reaction was stopped using 5 µL 40 µM EDTA in 1X LANCE Detection buffer (Perkin Elmer, CR97-100) for 5 min ...
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bioRxiv - Immunology 2022Quote: ... 5 μL of the IgG supernatants were transferred to an OptiPlate-384 assay plate (white opaque, PerkinElmer, Waltham, MA), mixed with 10μL (10 nM final conc. ...
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bioRxiv - Plant Biology 2019Quote: ... 200 uL from the methanol:chloroform extract was then combined with 5 mL of scintillation fluid (HIONIC FLUOR, Perkin Elmer). The remaining extract was removed from the residual biomass and water (2 mL ...
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bioRxiv - Physiology 2019Quote: ... and data collected on the somites 17-18 near the anal vent on a LSM 5 Exciter microscope (Zeiss) equipped with 20x/1.0W objective and subsequently processed using either Volocity 6.3 (Perkin Elmer), Fiji (NIH ...
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bioRxiv - Immunology 2021Quote: ... Immunohistochemistry was performed in Tris/NaCl blocking buffer (0.1□M Tris-HCl, 0.15□M NaCl, 5□μg/ml blocking reagent (Perkin Elmer), pH 7.5 ...
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Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. colibioRxiv - Microbiology 2020Quote: Autophosphorylation of YegI and point mutants was performed by addition of 5 μCi of [γ-32P] ATP (Perkin Elmer) to 0.2 μM of purified YegI or YegI point mutants in 10 μl of reaction buffer containing 50 mM Tris pH 7.5 ...
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bioRxiv - Cancer Biology 2022Quote: ... and maintained in 2.5 mL of KSFM supplemented with 5% (v/v) charcoal-stripped FBS and 50 nM R1881 (PerkinElmer) 25 ...
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bioRxiv - Immunology 2022Quote: ... 5% CO2 the luciferase activity was measured on Centro LB 960 Microplate Luminometer (Berthold Technologies) using Britelite PlusTM (PerkinElmer) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... with a total length of 109 ntb was prepared by 5′-end-labelling using [γ-32P]- ATP (Perkin Elmer) and T4 polynucleotide kinase (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Purified RNA oligos were either phosphorylated with unlabeled ATP or 5’ end-labeled using [γ-32P] ATP (Perkin Elmer) and T4-Polynucleotide kinase (New England Biolabs) ...
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bioRxiv - Biochemistry 2024Quote: ... The dry filter papers were placed in scintillation vials with 5 ml Ultima Gold F scintillation cocktail (Perkin Elmer) and scintillation was measured in a TriCarb 2900TR liquid scintillation analyzer (Perkin Elmer).
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bioRxiv - Genomics 2023Quote: ... The metabolite extract was transferred into scintillation vials with 5 mL Insta-Gel Plus scintillation cocktail (Perkin Elmer #601339).
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bioRxiv - Neuroscience 2024Quote: ... were collected from the CA1 SR from ≥5-7 sections per slice and processed for iterative deconvolution (99% confidence; Volocity 4.0, PerkinElmer). Three dimensional (3-D ...
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bioRxiv - Developmental Biology 2024Quote: ... The heads were rinsed five times in PBST at room temperature and then incubated in 50x diluted Cy3 Tyramide Reagent (PerkinElmer Life Science, Inc. 1:50 dilution) in Amplification Dilution buffer for 90 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... the plates containing infected macrophages were incubated at 37°C with 5% CO2 for 18 h in an Operetta High-Content Imaging System (PerkinElmer). During this 18 h incubation ...
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bioRxiv - Developmental Biology 2021Quote: ... the cells were incubated for 5 minutes at 37°C with 55µCi/well of 35S-L-methionine and 35S-L-cysteine Promix (Perkin Elmer) in a minimal volume of culture medium ...
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bioRxiv - Cell Biology 2022Quote: ... cell motility was acquired using temperature (37°C) and CO2 (5%) controlled Operetta CLS High-Content Analysis System (PerkinElmer, HH16000000) (non-confocal ...
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bioRxiv - Molecular Biology 2019Quote: ... was first deprotected and phenol-chloroform extracted and then 5’ end radiolabeled with [γ-32P] ATP (PerkinElmer, 6000 Ci/mmol) using T4 PNK (New England Biolab or Thermofisher ...
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bioRxiv - Molecular Biology 2019Quote: ... The cells were then incubated with 5 nM [3H]-E2 (specific activity 87.6 Ci/mmol; PerkinElmer Life Science, Waltham, MA) in the presence of increasing concentrations of 2’ ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Spectra were analysed using OPUS 6.5 software (Bruker) and compared with RMIT University’s spectral library of organic compounds generated using Spectrum 10 software (Perkin Elmer). Fingerprint spectra shown in Figure 4D were prepared using GraphPad Prism.
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bioRxiv - Microbiology 2021Quote: ... 1.37 μg/mL of RBD and 5 μg/mL of Ecto) and incubated overnight in protein-binding 96-well plates (PerkinElmer). The next day ...
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bioRxiv - Neuroscience 2021Quote: ... plates were centrifuged once more under same conditions mentioned above and 5 µL of each FRET antibody pair was added (2.5 nM final concentration for donor and 5 nM for acceptor, diluted in 1x Lance buffer, (Perkin Elmer)) ...
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bioRxiv - Microbiology 2021Quote: ... and fluorescence at 355 nm excitation and 460 nm emission was monitored every 5 minutes for 50 minutes using an Envision plate reader (PerkinElmer). Rate of substrate cleavage was determined using linear regression of the raw data values obtained during the time course ...
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bioRxiv - Microbiology 2022Quote: ... was phosphorylated in vitro with His-MBP-PknBMtb at 0.015 μg/μl for 30 minutes at room temperature with 0.15μCi/μl ATP [γ-32P] (5×10-8 μmol) (3000Ci/mmol, 10mCi/ml, PerkinElmer BLU002A250UC) and 2mM MnCl2 in buffer (50mM Tris ...
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bioRxiv - Neuroscience 2022Quote: ... Nifene or nicotine respectively for 5 min followed by addition of 2.5 nM [125I]-epibatidine ([125I]Epb) (2200 Ci/mmol; Perkin Elmer) and incubation for 20 min at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... Mice were injected IP with luciferin (5 mg/mouse; Gold Technologies) and then imaged on a Xenogen IVIS Spectrum imager (PerkinElmer) 10-15 minutes later for 60 seconds ...
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bioRxiv - Cancer Biology 2022Quote: ... Mice were injected IP with luciferin (5 mg/mouse; Gold Technologies) and then imaged on a Xenogen IVIS Spectrum imager (PerkinElmer) 10 minutes later ...
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bioRxiv - Cancer Biology 2022Quote: ... in 100-µL sterile PBS were injected into the left cardiac ventricle of female 5-week-old NSG mice as described previously (50) and in vivo imaging was performed (IVIS Spectrum, PerkinElmer) immediately to verify widespread seeding of tumor cells ...
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bioRxiv - Molecular Biology 2020Quote: ... with the confocal setting at 40X magnification at 37°C and 5% CO2 and analyzed with Columbus Image Analysis System (PerkinElmer). Each well was excited with 425 nm light and emissions detected at 434-515 nm for CFP and 500-550 nm for YFP ...
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bioRxiv - Plant Biology 2021Quote: ... The pellet was resuspended in 100 μl PIB and transferred to a scintillation vial containing 5 ml Ultima Gold XR Scintillation liquid (PerkinElmer). Radioactivity for each vial was measured by liquid scintillation spectrometry.
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bioRxiv - Cancer Biology 2020Quote: ... The filters were washed with 5 ml wash buffer and counted using a liquid scintillation counter (Wallac 1450 MicroBeta Trilux, PerkinElmer). Exchange reactions with GST-Rab35 on glutathione-Sepharose beads were performed in the presence or absence of Arf5 ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were grown for imaging using standard conditions and imaged in low fluorescence medium containing 5 µg/ml Dextran Alexa Fluor 647 on an Opera Phenix (PerkinElmer) automated system as described above.
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bioRxiv - Biochemistry 2020Quote: ... was incubated with 5 μL of biotinylated peptides at 100 nM for 30 min at room temperature in the epigenetics assay buffer (PerkinElmer AL008 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Emission intensity was recorded for 5 min using 460 nm and 530 nm filters on the EnVision or Victor X Light plate reader (PerkinElmer). Next ...
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Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. colibioRxiv - Microbiology 2020Quote: ... Reactions were incubated at 25 °C for 10mins followed by addition of 5 μCi of [γ-32P] ATP(Perkin Elmer). Reactions were incubated for additional 30 mins at 37 °C and were stopped using 3X Laemmli buffer ...
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bioRxiv - Genomics 2022Quote: ... the slides were washed three times in 1x TNT at RT for 5 minutes each and incubated with TSA Plus Fluorescein (PerkinElmer) according to the manual at RT for 3-15 minutes (3-5 min in H ...