Labshake search
Citations for PerkinElmer :
1401 - 1450 of 1456 citations for 4 Methoxy 2 2 3 4 5 5 Hexacb Unlabeled 50 Ug Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2020Quote: ... Fifty μl of each elution fraction was suspended in 1 ml of liquid scintillation reagent (Ultima Gold, PerkinElmer, Wellesley, MA, USA), and the radioactivity in each vial was measured using a scintillation counter ...
-
bioRxiv - Cell Biology 2021Quote: ... incubated at 37°C for 30 min in 500 μl starvation medium (DMEM lacking methionine and cysteine) and another 30 min in 500 μl labeling medium containing 100 μCi/ml [35S]methionine/cysteine (Perkin Elmer). The cells were washed twice with PBS and lysed in 500 μl lysis buffer (PBS with 1% Triton X-100 ...
-
bioRxiv - Microbiology 2020Quote: ... log phase promastigotes were washed twice with PBS and resuspended at 5.0 x 107 cells/ml in PBS in the presence of 100 µM of D-[U-14C] glucose (250 mCi/mmol, PerkinElmer Inc.). The reaction mixture (200 µl each in triplicates ...
-
bioRxiv - Plant Biology 2020Quote: ... the remaining root parts were collected and ground completely in liquid nitrogen and homogenized in 1 mL scintillation solution (PerkinElmer, 6013199). The samples were incubated overnight to allow the radioactivity to evenly diffuse into the whole volume of the scintillation cocktail ...
-
bioRxiv - Cell Biology 2021Quote: ... The cells were pulsed with 0.1 mCi/ml 35S-L-methionine/35S-L-cysteine mix (EasyTag™ EXPRESS35S Protein Labeling Mix, Perkin Elmer) in cysteine/methionine-free DMEM for 15 min at 37°C ...
-
bioRxiv - Cell Biology 2020Quote: ... the cells were pulsed with 0.1 mCi/ml 35S-L-methionine/35S-L-cysteine mix (EasyTag™ EXPRESS35S Protein Labeling Mix, Perkin Elmer) in serum-free ...
-
bioRxiv - Microbiology 2020Quote: ... cells were metabolically pulse labeled with methionine- and cystine-free DMEM supplemented with 70 μCi/ml 35S-labelled Met/Cys (EasyTag EXPRESS35S, Perkin Elmer). Total protein was boiled in SDS sample buffer and then either fractionated by SDS PAGE and exposed to film or precipitated in 10% Trichloroacetic acid (TCA ...
-
bioRxiv - Neuroscience 2021Quote: ... was added to the chamber for 30 min to inhibit transcription after which slices were transferred to fresh aCSF containing ~10 mCi/ml [35S] Met/Cys (Perkin Elmer) for an additional 30 min ...
-
bioRxiv - Microbiology 2020Quote: ... streptavidin donor beads were added (20 μg/ml) and the mixtures were incubated for 30 mins and were then measured with the Ensight multimode reader (Perkin Elmer) using excitation wavelength of 680 nm with donor beads and measurement of emission wavelength of 615 nm from AlphaLISA anti-HIS acceptor beads ...
-
bioRxiv - Immunology 2021Quote: ... dissolved in PBS (150 μg/mL) 10min before analysis performed using the IVIS® Lumina II platform (PerkinElmer, Waltham, MA, USA). Photons emitted from luciferase-expressing cells in selected regions of interest (ROI ...
-
bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% dialyzed fetal calf serum and then metabolically labelled for 10 min with 55 μCi/ml 35S-methionine/cysteine protein labeling mix (Perkin Elmer). Cells were rinsed immediately with PBS twice and solubilized with 1% Triton X-100 in solubilization buffer (50 mM HEPES-KOH ...
-
bioRxiv - Neuroscience 2020Quote: ... and incubated 10 minutes at 37 °C by addition of 1 μCi/ml of L-[3,4-3H]-Glutamic acid (Perkin Elmer, NET490) and 10µM L-glutamate (Sigma) ...
-
bioRxiv - Cell Biology 2020Quote: MCF10A cells transfected with control or ErbB3 siRNA were incubated with growth factor deprived DMEM media lacking methionine and cysteine supplemented with 10 μCi/ml 35S-methionine/cysteine (Perkin Elmer) for 30 minutes at 37°C ...
-
bioRxiv - Microbiology 2020Quote: ... rabbit reticulocyte lysate was incubated with 1μg of the appropriate expression plasmid and 2mL of [35S] L-methionine (1,000 Ci/mMol at 10mCi/mL) (Perkin Elmer, NEG709A) for 90 minutes at room temperature ...
-
bioRxiv - Immunology 2021Quote: ... Liver luminescence was assessed 42 hours post-challenge by intraperitoneally injecting anesthetized mice with 100 μl D-luciferin (30mg/ml) and then determining liver luminescence using an IVIS Spectrum Imaging System (Perkin Elmer). Beginning four days after challenge ...
-
bioRxiv - Cell Biology 2020Quote: ... subconfluent HDLECs cultured in 12-well plates were incubated with 1 ml per well EBM2 medium (containing appropriate amounts of serum and supplement) with [6-14C]-glucose (Perkin Elmer) for 6 hours ...
-
bioRxiv - Immunology 2022Quote: ... Paracellular permeability was determined by measuring mucosal-to-serosal flux (%flux/cm2/hour) of the small inert probe 51Cr-EDTA (360 Da; 6 μCi/mL) (Perkin Elmer), by taking an initial radiolabelled sample of mucosal buffer and then samples from the serosal compartment every 30 minutes for 2 hours ...
-
bioRxiv - Microbiology 2022Quote: 643nt pre-crRNA was in vitro transcribed with [a-32P]-ATP (10 mCi ml-1, 3000 Ci mmol-1, Perkin Elmer) as described above ...
-
bioRxiv - Neuroscience 2022Quote: ... 0.5 mL the top aqueous layer was used to count the radioactivity using a Tri-Carb 2800TR Liquid Scintillation Analyzer (Perkin Elmer). 0.5 mL medium at time point 0 was used as a control to determine the specific activity of the buffer.
-
bioRxiv - Plant Biology 2023Quote: ... Stained SDS-PAGE bands were excised and transferred to scintillation vials and emulsified in 10 ml of Ultima Gold XR scintillation liquid (Perkin Elmer) in darkness at room temperature overnight ...
-
bioRxiv - Bioengineering 2022Quote: ... samples were washed in lg-DMEM and samples were stained overnight at 37 °C in osteogenic control medium (Section 2.2.1) with 0.2 nmol/ml OsteoSense™ 680 (dissolved in PBS; NEV10020EX, PerkinElmer, Waltham, MA, USA) to visualize hydroxyapatite ...
-
bioRxiv - Molecular Biology 2024Quote: ... and 50 ng was used for random priming reactions in the presence of the radiolabeled nucleotide [α-32P]-dCTP (3,000 Ci/mmol, 10 mCi/ml, Perkin Elmer) using a random primer DNA labeling kit (TaKaRa ...
-
bioRxiv - Neuroscience 2024Quote: ... Cells were counterstained with DAPI (1 μg ml-1) before imaging with the Opera Phenix High Content Screening System (Perkin Elmer).
-
bioRxiv - Neuroscience 2023Quote: ... the biosensor cells were plated at a density of 0.075 x 106 cells/mL in a CellCarrier 96-well plate (PerkinElmer, Cat. # 6005550). As described above ...
-
bioRxiv - Neuroscience 2023Quote: Biosensor cells were plated at a density of 0.15 x 106 cells/mL in a CellCarrier 96-well plate (PerkinElmer, Cat. # 6005550). Cells were transfected with 50 nM R2/R3 fibrils premixed in Opti-MEM™ I Reduced Serum Medium supplemented with P3000™ reagent (0.25 µL/well ...
-
bioRxiv - Cancer Biology 2024Quote: ... with the luciferase substrate D-luciferin (200µL of 15mg/ml - assuming an average animal weight of 24-26gr) (Perkin Elmer, 122799) and incubated for 10 minutes at room temperature before images were taken using IVIS Spectrum In Vivo Imaging System (Perkin Elmer) ...
-
bioRxiv - Cell Biology 2023Quote: ... the cells were pulsed with 0.1 mCi/ml 35S-L-methionine and 35S-L-cysteine mix (EasyTag™ EXPRESS35S Protein Labeling Mix, Perkin Elmer) in methionine- and cysteine-free DMEM for 15 min ...
-
bioRxiv - Microbiology 2023Quote: ... the challenged mice were intraperitoneally injected with 100 μL of D-luciferin (30 mg/mL) (Perkin Elmer, Waltham, MA, USA, #122799), and then anesthetized in an isoflurane chamber ...
-
bioRxiv - Biochemistry 2023Quote: MLL1C binding assays (Figure 2C) were performed as above except using Nickel-chelate acceptor beads (10 μg/mL; Perkin Elmer AL108M), streptavidin donor beads (20 μg/mL ...
-
bioRxiv - Developmental Biology 2023Quote: ... Paracellular permeability was assessed by mucosal-to-serosal flux (%flux/cm2/hr) of the small inert probe 51Cr-EDTA (360 Da; 6 μCi/mL) (Perkin Elmer), by taking an initial radiolabelled sample of mucosal buffer and then samples from the serosal compartment every 30 minutes for 2 hours ...
-
bioRxiv - Microbiology 2023Quote: ... cells were washed twice with methionine-free DMEM and labeling media was prepared by adding 75 uCi of [35S] methionine per mL (PerkinElmer, NEG709A) of methionine-free medium (Gibco) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Dissociated cells were resuspended in 7.5 mg/mL BME-2 matrix gel at 2-5×104 cells/mL and seeded in 10 μL droplets to individual wells of 96-well Cell Carrier Ultra plates (Perkin Elmer). Once settled ...
-
bioRxiv - Cancer Biology 2024Quote: Luminescence imaging was carried out on days indicated using an IVIS luminescence imaging system 10 min after mice were injected with 10 µl/g of body weight 15 mg/mL in vivo grade D-Luciferin (PerkinElmer, 122799). Mice were sacrificed when reaching AAALAC-defined endpoints by CO2 asphyxiation followed by cervical dislocation ...
-
bioRxiv - Cell Biology 2020Quote: Posttranslational labeling was performed by incubating cells in normal growth medium containing 400 mCi/mL [3H]glutamine (PerkinElmer, 30-60 Ci/mmol) in the presence of (100 µg/ml ...
-
bioRxiv - Microbiology 2022Quote: ... we collected 22 mL of gas from each jar and analyzed it using a Clarus 400 gas chromatograph (PerkinElmer Ltd., Beaconsfield, UK) equipped with an automatic split/splitless injector and a flame ionization detector (FID) ...
-
bioRxiv - Cancer Biology 2020Quote: ... proteins were first labeled for 48 h with 0.25 μM Ci ml-1 L-[U-14C] VALINE (Perkin Elmer, Waltham, MA, USA) in GIBCO-RPMI 1640 medium (Invitrogen ...
-
bioRxiv - Bioengineering 2020Quote: ... Levels of luciferase production from glioblastoma cells were measured using D-luciferin (150 μg/ml final concentration, Perkin Elmer, Waltham, MA, USA) and luminescence measurement in a plate reader after 15 minutes ...
-
bioRxiv - Cell Biology 2022Quote: ... HEK293T cells transfected with FTTTRA25T and were labeled for 30 min with EasyTag Express [35S] Protein Labeling Mix (0.1 mCi/mL; Perkin Elmer CAT# NEG772014MC) in DMEM lacking both Cys and Met (Gibco CAT# 21013024 ...
-
bioRxiv - Cell Biology 2021Quote: ... media was removed and replaced with serum-free Modified Eagles Medium (MEM; Mediatech) containing 1 µCi/ml of 3H-myo-inositol (PerkinElmer Life Sciences). Forty-eight hrs post-infection ...
-
bioRxiv - Biochemistry 2021Quote: ... metabolic labeling was started by replacing the medium with phosphate-free DMEM containing 10% dialyzed FBS and 1mCi/mL 32P orthophosphate (PerkinElmer, Waltham, MA). After labeling for 8 hours ...
-
bioRxiv - Cancer Biology 2024Quote: ... Mice were injected IP with 10 µL/g of body weight of 15 mg/mL in vivo grade D-Luciferin (luciferin; PerkinElmer, Waltham, MA), anesthetized with 2-3% isoflurane in an induction chamber ...
-
bioRxiv - Cancer Biology 2022Quote: ... was added to a final concentration of 150 μL/mL and luciferase signal was measured using the Envision® 2105 Multimode Plate Reader (Perkin Elmer). Alternatively ...
-
bioRxiv - Molecular Biology 2023Quote: ... GLuc production was measured as a surrogate for IFN-β promoter activation by adding 25 µl coelenterazine-solution (1 µg/ml in H2O) was to 25 µl supernatant.reader (Perkin Elmer, Waltham, USA) and luminescence was measured ...
-
bioRxiv - Bioengineering 2021Quote: ... The volume of samples was adjusted to 10 mL by adding ultra-pure water and Gd concentration in each sample was measured using ICP-Mass Spectrometer (PerkinElmer, NexION® 2000). A similar procedure was used for monolayer cell culture (the cells were rinsed with PBS (3x 5 min ...
-
bioRxiv - Microbiology 2020Quote: ... at a final MTT concentration of 0.45 mg/ml or by use of a ATPlite Luminescence Assay System (PerkinElmer, Waltham, Massachusetts, United States) according to the manufacturer’s instructions.
-
bioRxiv - Cancer Biology 2021Quote: ... sedated mice were injected with 100 μl (30 mg/ml D-luciferin) in an isoflurane administrating chamber and then placed in an IVIS Lumina III XR machine (Perkin Elmer, Norwalk, CT). IVIS values on day three post tumor implantation were taken to allocate mice into balanced treatment groups of PBS-injected ...
-
bioRxiv - Cancer Biology 2022Quote: ... Organoid/hydrogel cultures were serum starved overnight before treatment with EGF (20 ng/mL) and promegestone/R5020 (1, 10, 100 nM; Perkin Elmer, Cat. #: NLP00400) and 10 nM R5020 with or without the addition of FAK (1 μM ...
-
bioRxiv - Biophysics 2019Quote: ... The solutions were transferred to a 2 mL disposable plastic cuvette and absorbance was measured using a spectrophotometer at a wavelength of 833 nm (Lambda XLS, Perkin Elmer, Hamburg, Germany). The blank value was deducted from the standard solutions and a calibration curve was prepared by plotting absorbance against the amount of phospholipid (micromoles) ...
-
bioRxiv - Immunology 2020Quote: ... luciferase expressing target cells were transferred to a white round-bottomed 96-well plate in the presence of Xenolight D-Luciferin (75 μg/mL; Perkin Elmer, Oslo, Norway). CAR-expressing T cells were subsequently added in the mix and cells were cultured in an incubator (37 °C in 5% CO2 ...
-
bioRxiv - Genetics 2019Quote: ... evaporated under nitrogen gas (Organomation Associates, Inc., Berlin, MA, USA) and resuspended in 10 mL Insta-Gel Plus scintillation fluid (Perkin Elmer, Cat#6013399), followed by counting with a Beckman LS6500 scintillation counter ...