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Citations for PerkinElmer :
1351 - 1400 of 1467 citations for 5 1 3 benzothiazol 2 ylamino pentanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... was next added and incubated with the mixture at 4 °C for 1 hour before the measurements performed on Envision Readers (PerkinElmer). The fluorescently-labeled m6A-containing mRNA was used to adjust the gain factor ...
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bioRxiv - Cancer Biology 2023Quote: ... Luminescence measurements (photons per second per cm2) were acquired over 1 minute and analysed using the Living Image software (PerkinElmer) using a constant size region of interest over the tissues ...
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bioRxiv - Biochemistry 2022Quote: ... 300 mM potassium acetate and 1 mM DTT followed by addition of 10 μM acetyl-CoA in a 1:10 ratio of 3H radiolabeled acetyl-CoA (PerkinElmer) to cold acetyl-CoA ...
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bioRxiv - Biochemistry 2023Quote: ... Reactions were initiated by adding an equal volume of assay buffer containing 1 μM [35S]GTPγS (~50 cpm/fmol, Perkin Elmer) at 30 °C ...
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bioRxiv - Plant Biology 2023Quote: ... RNA quantification was performed through spectrophotometric analysis at 260 nm using the Nanodrop ND-1000 Spectrophotometer and RNA quality was assessed using 1% agarose gel electrophoresis or the LabChip GX Touch (PerkinElmer). 5 µg of purified total RNA was combined with 5 µL of TURBO DNase buffer and 1 µL TURBO DNase (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were washed 5x with TNT buffer and the second florescent signal was detected by incubating the samples in Cy5-TSA diluted 1:50 in Diluent buffer (PerkinElmer). The samples were washed at least 4x for 15 minutes each wash and then stained with Hoechst at 4°C overnight on a shaker before being put into Scale A2.
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bioRxiv - Microbiology 2023Quote: Brine samples BP3 and BP4 were spiked with L-[4,5-3H] leucine (Perkin Elmer, specific activity 100 Ci mmol-1) at a final concentration of 20 nmol leucine L-1 (Kirchman et al. ...
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bioRxiv - Cell Biology 2024Quote: ... using a HyBEZ® oven Probes were detected using Tyramide signal amplification (TSA) reaction (1:600 dilution) using an Alexa-flour labeled TSA kit (PerkinElmer) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... the reaction was stopped with an equal volume of 1 M H2S04 and absorbance at 450 nm was quantified on a Victor X4 plate reader (PerkinElmer). Values reported are A450 means ± standard deviations across all dilutions falling within a valid calibration curve range that are at least two-fold above background and no more than 2.8.
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bioRxiv - Cell Biology 2024Quote: ... cells were changed into 1 mL of starvation media containing 200 μCi/mL EasyTag EXPRESS35S Protein Labeling Mix (PerkinElmer NEG772002MC), grown for indicated time points ...
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bioRxiv - Neuroscience 2024Quote: ... the membranes were incubated for 90 seconds in 1 mL of enhanced chemiluminescence (ECL Pro) reagent (catalog number NEL122001EA, PerkinElmer Health Sciences Canada Inc. ...
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bioRxiv - Biochemistry 2024Quote: ... supplemented with 10% FBS and 1% Sodium pyruvate and seeded at a density of 30,000 cells per well in Isoplate-96 plates (Perkin Elmer). Twenty-four hours after transfection with the wild-type or mutant hURAT1 ...
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bioRxiv - Biophysics 2021Quote: ... Depletion of NADPH is observed in 1 mL cuvettes with a path length of 1cm in a Lambda 650 UV/VIS spectrometer (Perkin Elmer) with attached water Peltier system set to 17°C ...
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bioRxiv - Cell Biology 2020Quote: ... Embryos were then washed in Maleic buffer and then PBS at room temperature prior to incubation with 50 µl amplification diluent with 1 µl Tyramide-Cy3 (Perkin Elmer) for 3 h at room temperature in the dark ...
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bioRxiv - Cell Biology 2020Quote: ... the wells were pulsed with 1 μCi/well of [methyl-3H]TdR (70–90 Ci/mmol; NEN Radiochemicals, PerkinElmer, Akron, OH). After 16 h ...
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bioRxiv - Developmental Biology 2021Quote: ... Heat mediated antigen retrieval was used to process sections (Vector, H-3300) and then samples were blocked for 1 hour in blocking buffer at room temperature (PerkinElmer, FP1020). Primary antibodies diluted in blocking buffer were incubated with samples overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... LPS-stimulated plasmablasts were starved for methionine and cysteine for 60 minutes and pulse labeled for 15 minutes with 55 µCi/1×106 cells of Express 35S protein labeling mix (Perkin Elmer). Samples were then diluted 10x with cell culture media containing 5mM unlabeled methionine and cysteine ...
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bioRxiv - Genomics 2020Quote: ... 63%, 40%, 25%) with 9:1 CSBL1:CBL1 buffer (Zeptosens, Bayer) using a Sciclone Caplier ALH3000 liquid handling root (Perkin Elmer). Diluted samples were spotted onto ZeptoChips (Zeptosens ...
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bioRxiv - Genetics 2021Quote: Cells were fixed in growth medium with 3.7% formaldehyde at room temperature for 30 minutes and their cell walls digested with a solution containing 50 μg/ml zymolyase and 1:50 glusulase (Perkin Elmer). The digested cells were mounted on poly-L lysine coated slides to immobilize them for microscopy ...
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bioRxiv - Genomics 2020Quote: ... 5 mM MgCl2, 1 mM DTT, 300 mM KCl, 40 µM ATP, 40 µM GTP, 40 µM Biotin-11-CTP [Perkin Elmer] ...
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bioRxiv - Immunology 2021Quote: TZM-bl target cells (NIH AIDS reagent program) were seeded at a density of 1×104 cells/well in 96-well luminometer-compatible tissue culture plates (Perkin Elmer) 24h before infection ...
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bioRxiv - Microbiology 2021Quote: ... 1 mL of cells from each culture pulsed with 1 μCi [γ32P]-ATP having a specific activity of 30 Ci/mmol (Perkin Elmer) for 5 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... 293T-ACE2 target cells were seeded at a density of 1×104 cells/well in 96-well luminometer-compatible tissue culture plates (Perkin Elmer) 24h before infection ...
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bioRxiv - Biochemistry 2020Quote: ... Fifty μl of each elution fraction was suspended in 1 ml of liquid scintillation reagent (Ultima Gold, PerkinElmer, Wellesley, MA, USA), and the radioactivity in each vial was measured using a scintillation counter ...
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bioRxiv - Microbiology 2021Quote: ... 293T-ACE2 target cells were seeded at a density of 1×104 cells/well in 96-well luminometer-compatible tissue culture plates (Perkin Elmer) 24h before infection ...
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bioRxiv - Biochemistry 2021Quote: ... and mRNA was translated for 1 hour at 30 °C in presence of 10 µCi/µL EasytagTM 35S Express Protein Labeling Mix (Perkin Elmer). The mixtures were centrifuged for 2 min at 10,000 x g at 4 °C after inhibiting protein synthesis with 1 mM cycloheximide and translocated CFTR was retrieved from the pellet fraction ...
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bioRxiv - Plant Biology 2020Quote: ... the remaining root parts were collected and ground completely in liquid nitrogen and homogenized in 1 mL scintillation solution (PerkinElmer, 6013199). The samples were incubated overnight to allow the radioactivity to evenly diffuse into the whole volume of the scintillation cocktail ...
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bioRxiv - Microbiology 2020Quote: ... 293T-ACE2 target cells were seeded at a density of 1× 104 cells/well in 96-well luminometer-compatible tissue culture plates (Perkin Elmer) 24 h before infection ...
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bioRxiv - Microbiology 2020Quote: ... target cells were seeded at a density of 1×104 cells/well in 96-well luminometer-compatible tissue culture plates (Perkin Elmer) 24 hours before infection.
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bioRxiv - Molecular Biology 2022Quote: ... 1mM PMSF, 100mM KCl and 1 mCi of [3H]AdoMet (S-adenosyl-L-[methyl-3H] methionine, 80 Ci/mmol; SAM) (Perkin Elmer) or 0.15mM unlabeled S-adenosyl methionine for 45 min at 30°C ...
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bioRxiv - Microbiology 2020Quote: ... 293T-ACE2 target cells were seeded at a density of 1×104 cells/well in 96-well luminometer-compatible tissue culture plates (Perkin Elmer) 24h before infection ...
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bioRxiv - Microbiology 2020Quote: ... [methyl-14C] CDP-choline (50–60 mCi/mmol) and [diarachidonyl-1-14C] PC (50–60 mCi/mmol) were purchased from Perkin Elmer, Inc (Waltham ...
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bioRxiv - Immunology 2021Quote: ... 293T-ACE2 target cells were seeded at a density of 1×104 cells/well in 96-well luminometer-compatible tissue culture plates (Perkin Elmer) 24h before infection ...
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bioRxiv - Neuroscience 2021Quote: ... The signal of CCK hybridization was revealed by a TSA reaction using fluorescein isothiocyanate (FITC)-labeled tyramide (1:100 for 10 minutes, NEL741001KT Perkin Elmer). After several washes ...
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bioRxiv - Cancer Biology 2020Quote: ... membranes were washed and probed with horseradish peroxidase (HRP)-conjugated secondary antibodies for 1 hour before detection with Western Lightning Chemiluminescence (Perkin Elmer). Antibodies used included ...
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bioRxiv - Microbiology 2021Quote: ... 293T-ACE2 target cells were seeded at a density of 1×104 cells/well in 96-well luminometer-compatible tissue culture plates (Perkin Elmer) 24h before infection ...
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bioRxiv - Synthetic Biology 2020Quote: ... Each reaction was diluted in TE and mixed with a PicoGreen working stock (diluted to 1/200× in TE Buffer) to be read with an Enspire plate reader (Perkin Elmer) with 480 nm excitation and 520 nm emission filter ...
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bioRxiv - Immunology 2021Quote: ... target cells were seeded at a density of 1 × 104 cells/well in 96-well luminometer-compatible tissue culture plates (Perkin Elmer) 24 h before infection ...
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bioRxiv - Microbiology 2020Quote: ... 293T-ACE2 target cells were seeded at a density of 1×104 cells/well in 96-well luminometer-compatible tissue culture plates (Perkin Elmer) 24h before infection ...
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bioRxiv - Genomics 2021Quote: ... the anti-DIG-HRP antibody was detected with the TSA-Cy3 substrate (1:50, TSATM PLUS Cy3 kit, NEL 745001KT, Perkin Elmer) for 10 min at room temperature ...
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bioRxiv - Physiology 2020Quote: ... glycosylphosphatidylinositol-anchored high-density lipoprotein binding protein 1 (GPIHBP1) knock out mice were orally gavaged with 100 μCi of [9,10-3H(N)]-Triolein (Perkin Elmer, NET431001MC) suspended in olive oil ...
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bioRxiv - Cell Biology 2020Quote: ... subconfluent HDLECs cultured in 12-well plates were incubated with 1 ml per well EBM2 medium (containing appropriate amounts of serum and supplement) with [6-14C]-glucose (Perkin Elmer) for 6 hours ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then solubilized with 200μL of 1% SDS 0.2N NaOH and transferred to a scintillation vial with 5mL of Insta-Gel Plus scintillation cocktail (Perkin Elmer #601339). Radioactivity was measured with the TopCount scintillation counter (Perkin Elmer) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Analytical estimation of the stock CoCl2 solution concentration (100 mmol L−1) was performed employing inductively coupled mass spectrometry (Perkin Elmer Avio 200 optical emission spectrometer ...
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bioRxiv - Genetics 2024Quote: ... was done for 1 hour at room temperature and following the addition of the western lightening ECL Pro ECL (Perkin Elmer), images were then directly recorded using Biorad Molecular Imager® Chemi DocTM XRS ...
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bioRxiv - Biochemistry 2024Quote: ... 250 nM purified MBP-Naa80, 200 µM substrate peptide (Figure 1 and Supplemental Table S1, 50 µM 14C-Ac-CoA (Perkin Elmer) were mixed in acetylation buffer (50 mM Tris-HCl pH 8.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... the remaining 20% of the beads (supplemented with 40 µl of 2X ROTI®Load and boiled at 95°C for 10 min) were subjected to SDS-PAGE for immunoblotting together with 1% input samples using HRP-streptavidin conjugate (PerkinElmer; #NEL750001EA) or anti-p65 (Santa Cruz Biotechnology ...
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bioRxiv - Neuroscience 2024Quote: ... washed one time with PBS and one time with PBS+DAPI (1:10 000) and subsequently imaged on the Opera Phenix High Content Screening System (Perkin Elmer) on a 40X water objective ...
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bioRxiv - Bioengineering 2023Quote: UV-vis spectra of the medium and hydrogel samples were measured using a plastic cuvette (1 cm light path) with a UV-visible light spectrophotometer (Lambda 35, Perkin Elmer). The transmittance was measured over the range of 800-400 nm.
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bioRxiv - Cell Biology 2023Quote: ... and 1% antibiotic/antimycotic at 37°C for 30 min and then labeled with Express [35S] Labeling Mix (PerkinElmer, NEG072-007) for 15 min ...