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Citations for PerkinElmer :
1201 - 1250 of 1371 citations for 6 Bromo 3 N ethylamino 1 2 4 triazolo 4 3 a pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Molecular weight bands were visualized by incubation with peroxidase-conjugated anti-mouse or anti-rabbit secondary antibody (1:5000; Jackson Immuno Research Laboratories) followed by treatment with the ECL-plus reagent (Western Lighting; PerkinElmer) and exposure on G:Box Chemi XRQ system (Syngene) ...
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bioRxiv - Molecular Biology 2021Quote: ... Small RNA libraries were prepared from 1 ng of RNA using the NEXTflex Small RNA-seq Kit v3 (Perkin Elmer). The size distribution of the libraries was assessed on a Bioanalyzer with a DNA High Sensitivity kit (Agilent Technologies) ...
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bioRxiv - Molecular Biology 2021Quote: ... The radiolabeled probes (200 bp) were generated by PCR using (γ-32P) dCTP 0.1 mM (3000 Ci. mmol-1 PerkinElmer). The primes used were ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies were detected by incubation with peroxidase-conjugated anti-mouse and anti-rabbit antibodies (1:10000, Jackson Immuno Research Laboratories) followed by reaction with ECL-Plus reagent (Western Lighting, PerkinElmer) and exposure to autoradiography films ...
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bioRxiv - Biochemistry 2019Quote: RAW264.7 cells in 24-well plates were labeled by overnight incubation with DMEM containing 1% fetal bovine serum and 0.5 μCi/mL [3H]cholesterol (Perkin Elmer). The next day ...
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bioRxiv - Cancer Biology 2019Quote: ... 10 nmol of chemerin-9 in 25 μL iodination buffer (0.5 M sodium phosphate, pH 7.4) were mixed with 1 mCi carrier-free Na125I (NEZ033L010MC, PerkinElmer, Waltham, USA) in an HPLC glass vial with microvolume insert ...
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bioRxiv - Bioengineering 2020Quote: ... Azide functionalization was confirmed with Fourier-transform infrared spectroscopy (FTIR, Supplemental Figure 1, F Perkin Elmer Frontier MIR/NIR spectrometer) and quantified with electrospray ionization-mass spectrometry (ESI-MS ...
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bioRxiv - Neuroscience 2021Quote: ... 1×104 HEK-293T cells were seeded in culture medium without antibiotics in white 96-well culture plates (Perkin Elmer) prior to transfection ...
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bioRxiv - Cancer Biology 2021Quote: ... HRP-conjugated secondary anti-rabbit and anti-mouse (1:5000) were used and detected by using the enhance Chemiluminescent Substrate (PerkinElmer). The bands were visualized using the ChemiDoc Imaging System (BioRad).
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bioRxiv - Molecular Biology 2022Quote: Undifferentiated H1-OCT4-GFP-INS:tdT-luciferase hPSC and the differentiated β-like cells were incubated with 15mg/mL D-luciferin for 1 min before imaging with an IVIS Spectrum system (PerkinElmer), with an exposure of 1 min and mid-bin setting ...
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bioRxiv - Microbiology 2022Quote: ... Infection was quantified after 24 h by FACS (for HIV-1-mCherry) or by adding 50 μl/well britelite plus reagent (PerkinElmer) and measurement on a Pherastar FS (BMG ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were washed in PBSTw and then incubated in FITC-Tyramide (Perkin-Elmer) 1:100 in amplification reagent (Perkin Elmer), followed by several washes in PBSTw ...
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bioRxiv - Neuroscience 2020Quote: Larval stage 1 animals (L1) were imaged in a UltraView VoX spinning-disc confocal microscope (PerkinElmer Life and Analytical Sciences). Embryonic imaging was performed via dual-view inverted light sheet microscopy (diSPIM)64,65 ...
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bioRxiv - Neuroscience 2021Quote: ... during 2 hours at RT followed by a 10 minutes incubation at RT with TSA plus Biotin System (Biotin TSA 1:250, NEL749A001KT PerkinElmer). After several washes ...
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bioRxiv - Neuroscience 2021Quote: ... during 2 hours at RT followed by a 10 minutes incubation at RT with TSA Biotin System (Biotin TSA 1:100, NEL700A001KT PerkinElmer). After several washes ...
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bioRxiv - Biochemistry 2020Quote: ... was transferred to 384-well plates and diluted to 1 mM in DMSO using a JANUS Varispan Automated Workstation (PerkinElmer). The U2AF1S34F–U2AF2–SF1 protein and FlDEK variant oligonucleotide were plated at a total 20 μL reaction volume per well ...
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bioRxiv - Cell Biology 2019Quote: A centrifuge-free RABiT-II system was used for automated sample processing after culturing: The system consists of two part: the first (Figure 1) has an automated liquid-handling system (JANUS, PerkinElmer) with integrated automated microplate handler (Twister3 ...
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bioRxiv - Systems Biology 2020Quote: ... the DIG-labeled probe was visualized using a tyramide-Cy3 Plus kit (1:50 dilution, 15-minute incubation, Perkin Elmer). Following washes in phosphate buffered saline (PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... Plates were incubated at RT for 1 h and the fluorescence intensity measured at 620 nm and 650 nm by an EnVision multilabel plate reader (PerkinElmer).
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bioRxiv - Plant Biology 2020Quote: ... or without (for pPcUBI (I) and (II)) 1 μM Brassinazole medium were imaged using an Ultra View Vox Spinning disc confocal imaging system (PerkinElmer) mounted on an Eclipse Ti inverted microscope (Nikon ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and 20 μl of the reaction mixture was incubated at 26°C for 1 h in a 384-well AlphaPlate (PerkinElmer). Subsequently ...
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bioRxiv - Neuroscience 2020Quote: ... anti-mouse or anti-rabbit secondary antibody (1:10000; Jackson Immuno Research Laboratories) followed by treatment with ECL-plus reagent (Western Lighting, PerkinElmer) and exposure to autoradiography films ...
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bioRxiv - Microbiology 2021Quote: ... an equivalent of 500 ng of p24 Gag was lysed in buffer with 1% Triton (ELISA kit, Alliance Perkin Elmer) for 30 min on ice ...
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bioRxiv - Biochemistry 2021Quote: ... microsomes prepared from HEK293 cells expressing RyR2 were incubated for 1 h at 25 °C with 5 nM [3H]ryanodine (PerkinElmer) in reaction media containing 0.17 M NaCl ...
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bioRxiv - Neuroscience 2021Quote: ... Three-plex detection of IL-4Ra on glutaminergic and GABAergic neurons was achieved using Opal 520 (1:1000, Perkin Elmer), Opal 570 (1:1500 ...
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bioRxiv - Genomics 2020Quote: ... These steps were repeated starting from blocking for the second staining with primary antibody rat anti-CD8a 1:300, secondary antibody goat anti-rat (MP-7444, Vector) and opal 690 (fp1497, PerkinElmer) and the third staining with rat anti-CD45 1:1,000 ...
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bioRxiv - Microbiology 2020Quote: ... which were exposed to X-ray film for 1 h and detected by a storage phosphor system (Cyclone, PerkinElmer, USA).
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bioRxiv - Microbiology 2020Quote: Overnight bacterial cultures were diluted 1:200 in LB broth and 50μl bacterial suspension was added per well in CellCarrier Ultra 96 plates (Perkin Elmer) and left in a static incubator at 37°C for 2 hours ...
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bioRxiv - Microbiology 2022Quote: ... The plate was then incubated for 1 h at RT in the dark before the AlphaLISA signal was detected using an EnSpire Multimode Plate Reader (PerkinElmer). Negative control with binding buffer or only with one of the complexes were used to control the assay quality ...
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bioRxiv - Microbiology 2022Quote: ... 293T-ACE2 target cells were seeded at a density of 1 × 104 cells/well in 96-well luminometer- compatible tissue culture plates (PerkinElmer) 24 h before infection ...
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bioRxiv - Biochemistry 2022Quote: ... Plates were incubated at RT for 1 h and the fluorescence intensity measured at 620 nm and 665 nm by an EnVision multilabel plate reader (PerkinElmer).
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bioRxiv - Biochemistry 2022Quote: ... Steady-state binding signal was obtained after incubation of reactions for 1 hr at 25° C using a EnSight Multimode plate reader (PerkinElmer) with a 30 ms excitation at 680 nm and emission measured at 615 nm after 140 ms ...
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bioRxiv - Immunology 2022Quote: ... and primary human GM-CSF monocyte derived macrophages (MDM) were cultured in collagen-1-coated CellCarrier Ultra 96-well black walled plates (PerkinElmer) at a ratio of 1425 HSC:28500 MDM in FBM (Lonza ...
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bioRxiv - Microbiology 2022Quote: The lipid compositions of the bacterial strains were determined after labeling with [1-14C] acetate (specific activity: 55 mCi/mmol; PerkinElmer) or [35S] sulfate (specific activity ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were washed three times in 1 x PBS and imaged using the Opera Phenix high-content microscope (Perkin Elmer) at 63x magnification.
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bioRxiv - Plant Biology 2023Quote: ... Radioactivity was measured from the extract and the trichloroacetic acid precipitated protein pellet for 5 min with a scintillation counter (Wallac 1414 WinSpectral v.1.40; Wallac Oy, Turku, Finland) using 1 ml of Ultima Gold™ (PerkinElmer) liquid scintillation cocktail ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Plates were incubated in the dark for 1 hr at 37°C followed by measurement of fluorescence using an Envision plate reader (PerkinElmer) with standard AlphaScreenTM settings ...
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bioRxiv - Bioengineering 2022Quote: ... and Ethidium homodimer-1 (10μM) and cell death and viability data were measured using Operetta CLS High Content Analysis System (PerkinElmer, UK).
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bioRxiv - Cancer Biology 2024Quote: ... mice were used for orthotopic transplantation of 1×105 secondary tumor NES (tNES) cells and tumor growth was monitored using IVIS SpectrumCT In Vivo Imaging System (PerkinElmer) as previously described [14] ...
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bioRxiv - Cancer Biology 2023Quote: ... Each protein (5 µL) was mixed and incubated for 1 h in a 384-well AlphaPlate (PerkinElmer, Waltham, MA, USA). After diluting Alpha Screen Protein A Acceptor Beads (PerkinElmer ...
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bioRxiv - Cancer Biology 2024Quote: ... Image acquisition and quantification of POPO™-1 iodide staining intensity were done using the Harmony high-content imaging and analysis software 4.8 (PerkinElmer). Mesothelial clearance was performed by seeding MeT5A-GFP cells (15 ...
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bioRxiv - Microbiology 2023Quote: ... Replication-competent HIV-1 stocks were normalized by p24Gag levels determined by ELISA (PerkinElmer Life Sciences, Inc., Boston, MA, USA).
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bioRxiv - Pathology 2023Quote: ... the p24 core antigen was detected directly in the viral supernatant with a HIV-1 p24 ELISA kit (Perkin Elmer), as specified by the supplier ...
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bioRxiv - Cancer Biology 2023Quote: ... Luminescence measurements (photons per second per cm2) were acquired over 1 minute and analysed using the Living Image software (PerkinElmer) using a constant size region of interest over the tissues ...
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bioRxiv - Biochemistry 2022Quote: ... 300 mM potassium acetate and 1 mM DTT followed by addition of 10 μM acetyl-CoA in a 1:10 ratio of 3H radiolabeled acetyl-CoA (PerkinElmer) to cold acetyl-CoA ...
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bioRxiv - Biochemistry 2023Quote: ... Reactions were initiated by adding an equal volume of assay buffer containing 1 μM [35S]GTPγS (~50 cpm/fmol, Perkin Elmer) at 30 °C ...
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bioRxiv - Plant Biology 2023Quote: ... RNA quantification was performed through spectrophotometric analysis at 260 nm using the Nanodrop ND-1000 Spectrophotometer and RNA quality was assessed using 1% agarose gel electrophoresis or the LabChip GX Touch (PerkinElmer). 5 µg of purified total RNA was combined with 5 µL of TURBO DNase buffer and 1 µL TURBO DNase (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were washed 5x with TNT buffer and the second florescent signal was detected by incubating the samples in Cy5-TSA diluted 1:50 in Diluent buffer (PerkinElmer). The samples were washed at least 4x for 15 minutes each wash and then stained with Hoechst at 4°C overnight on a shaker before being put into Scale A2.
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bioRxiv - Microbiology 2023Quote: Brine samples BP3 and BP4 were spiked with L-[4,5-3H] leucine (Perkin Elmer, specific activity 100 Ci mmol-1) at a final concentration of 20 nmol leucine L-1 (Kirchman et al. ...
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bioRxiv - Cell Biology 2024Quote: ... cells were changed into 1 mL of starvation media containing 200 μCi/mL EasyTag EXPRESS35S Protein Labeling Mix (PerkinElmer NEG772002MC), grown for indicated time points ...