Labshake search
Citations for PerkinElmer :
1201 - 1250 of 1408 citations for 2 1 ethylpentyl 1 3 dioxan 5 yl laurate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. colibioRxiv - Microbiology 2020Quote: ... Reactions were incubated at 25 °C for 10mins followed by addition of 5 μCi of [γ-32P] ATP(Perkin Elmer). Reactions were incubated for additional 30 mins at 37 °C and were stopped using 3X Laemmli buffer ...
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bioRxiv - Genomics 2022Quote: ... the slides were washed three times in 1x TNT at RT for 5 minutes each and incubated with TSA Plus Fluorescein (PerkinElmer) according to the manual at RT for 3-15 minutes (3-5 min in H ...
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bioRxiv - Genomics 2022Quote: ... the slides were washed three times in 1x TNT at RT for 5 minutes each and the amplification steps were repeated using anti-DNP-HRP Conjugate (PerkinElmer) and TSA Plus Cyanine 3 (PerkinElmer) ...
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bioRxiv - Immunology 2023Quote: ... Successful tumor implantation was confirmed on day 5 by luminescence detection using an IVIS® Lumina S5 Imaging System (PerkinElmer). Animals were then randomized and received an intracranial intratumoral administration of 1×106 anti-PTPRZ1 RNA CAR-T cells or Mock EP T cells in 2 μL of HBSS on day 7 ...
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bioRxiv - Immunology 2024Quote: ... the plates were centrifuged at 500 × g for 5 min and 20 µl of supernatant from each well was transferred to a 96 well DELFIA Yellow Plate (PerkinElmer) and combined with 200 µl of europium ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 0.05□μl of 5□mM stock compounds in DMSO were dispensed into assay microplates with a Janus 384 MDT NanoHead (PerkinElmer). Final concentration of the screening compounds in the assay was 16LμM ...
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bioRxiv - Neuroscience 2023Quote: ... Live cell imaging was performed at 37°C in 5% CO2 for 24 hours using the Opera Phenix High Content Screening System (PerkinElmer). Acquired images were analyzed by using the Harmony software (PerkinElmer).
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bioRxiv - Bioengineering 2023Quote: ... The plasma was obtained by centrifugation at 500 g for 5 min and measured for MFI using IVIS (PerkinElmer, USA).
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bioRxiv - Microbiology 2023Quote: ... Samples were mixed with the DNA absorption solution (for one sample: 50 uL 5 M NaCl, 15 ul magnetic beads (GMG-252-A-100mL – PerkinElmer), 250 uL 7.8 M guanidium chloride ...
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bioRxiv - Immunology 2023Quote: ... Tumor growth in live mouse was monitored every 5 days by bioluminescence imaging (BLI) using IVIS Spectrum In Vivo Imaging System (PerkinElmer) after 10mins intraperitoneally injection of 150mg/Kg D-luciferin ...
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bioRxiv - Immunology 2024Quote: ... of luciferase activity in each well was measured within 5 min after the addition of substrate using a Victor X4 plate reader (PerkinElmer). After subtracting background luciferase activity in uninfected cells ...
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bioRxiv - Immunology 2024Quote: ... Fluoforte® fluorescence (excitation 490 nM/emission 525 nM) was quantified at time 0 (baseline) and after 5 minutes on an Envision plate reader (PerkinElmer). Normalized relative fluorescence units (ΔRFU ...
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bioRxiv - Cell Biology 2024Quote: ... Tem1 (3.3 µM final concentration) was mixed with GTP (33 µM final) supplemented with 5 µCi [α-32P]-GTP (PerkinElmer) in the reaction buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Filters with the bound radioligand were transferred to vials containing 5 mL of Ultima Gold cocktail (PerkinElmer, Boston, MA, USA) and measured with a Packard Tri-Carb 2200CA liquid scintillation counter (PerkinElmer ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Microtubes were then centrifuged at 1000g for 5 min and radiolabeled lipids were counted in the upper phase with a Tri-Carb 4910TR liquid scintillation counter (PerkinElmer). Results were normalized to total protein content and expressed in comparison to control cells.
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bioRxiv - Microbiology 2024Quote: ... The bacterial mCherry signal was measured on day 0 and day 5 post infection on an Envision plate reader (PerkinElmer). Oleate was maintained throughout the experiment in the rescue assay conditions.
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bioRxiv - Systems Biology 2024Quote: ... They were then incubated with 0.5 ml of uptake solution containing [3H]glutamine (5 μCi/ml; Perkin Elmer, NET 55100) in either the presence ...
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bioRxiv - Immunology 2024Quote: ... diluted 1:40 in assay diluent 5 and then IL-1β levels were measured using the Human IL- 1β HTRF kit (Cisbio – PerkinElmer), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Microtubes were centrifuged at 1000g for 5 min and radiolabeled lipids were counted in the upper phase with a Tri-Carb 4910TR liquid scintillation counter (PerkinElmer). Results were normalized to cell number as described for oxygen consumption.
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bioRxiv - Genetics 2024Quote: ... Plates were incubated on a plate rocker for 5 min and luminescence read using a 400 to 700-nm filter equipped to an EnVision Plate Reader (PerkinElmer).
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bioRxiv - Biophysics 2024Quote: ... 750 µl of lysed cells) were transferred to a scintillation vial with 5 ml of scintillation cocktail (Perkin Elmer, 6013327). Radioactivity was measured with a liquid scintillation analyzer (Perkin Elmer ...
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bioRxiv - Cancer Biology 2024Quote: ... Metastatic outgrowth was longitudinally followed in vivo by intraperitoneal injection of luciferin (5 μg/kg) and imaging with IVIS Lumina III (PerkinElmer) (Garcia-Leon et al. ...
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bioRxiv - Neuroscience 2020Quote: ... animals were anesthetized (2 % isofluorane gas in O2) and luminescence was recorded (IVIS Spectrum, Perkin Elmer, Waltham, MA). Images were analyzed with Living Image Software (Caliper LifeSciences).
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bioRxiv - Plant Biology 2020Quote: ... N was quantified in approximately 2 mg subsamples using a CHN elemental analyzer (EA2400, PerkinElmer, Waltham, MA, USA). For phosphorus content ...
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bioRxiv - Molecular Biology 2020Quote: ... In brief, using a rabbit antimelatonin antiserum (PF1288; P.A.R.I.S., Paris, France) and 2-Iodomelatonin (NEX236050UC; PerkinElmer, Boston, Massachusetts) as tracer RIA was performed ...
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bioRxiv - Microbiology 2024Quote: ... 200 μl of each fraction was added to 2 ml of Ultima Gold liquid scintillation cocktail (Perkin Elmer), and the radioactivity was measured by a LS-6500 scintillation counter (Beckman) ...
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bioRxiv - Biophysics 2022Quote: ... and incubated at 40 C for 2 h before counting with a MicroBeta scintillation top counter (Perkin Elmer).
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed twice for 2 minutes with wash buffer and incubated in Opal 520 (Perkin Elmer FP1487A) or vivid 520 (ACDbio 323271 ...
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bioRxiv - Biochemistry 2023Quote: ... the beads were suspended in 25 µl of cytoplasmic buffer containing 2 µCi of 3H-labeled SAM (PerkinElmer) and incubated overnight at 32 °C without shaking ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed twice for 2 minutes with wash buffer and incubated in Opal 520 (Perkin Elmer FP1487A) for 30 minutes at 40°C ...
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bioRxiv - Molecular Biology 2024Quote: ... cAMP- dependent luminescence was then measured at 2-min intervals using a PerkinElmer Envision 2104 plate reader (PerkinElmer), as described [24] ...
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bioRxiv - Cell Biology 2021Quote: ... Images were acquired for 60 min at 1 frame/15-30 s using a spinning disk confocal microscope (UltraVIEW VoX, PerkinElmer with a 63X 1.4 N.A. oil objective). Images were analyzed using Volocity Suite (PerkinElmer ...
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bioRxiv - Cell Biology 2021Quote: ... Reactions were started by adding 40 µl IR in alkyl maltoside micelles to 10 µl radioactive kinase reaction buffer (0.4 mM ATP, 1 µCi [γ-32P]-ATP (PerkinElmer, 10 mCi/mL, 25-50 cpm/mol), 1 mM MgCl2 ...
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bioRxiv - Microbiology 2020Quote: ... the membranes were again washed three times for 5 min with TBS-T20 and the blots developed with Western lightning solution (Perkin Elmer) with a Fuji LAS-4000 imager (GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... Oligonucleotide probes (listed in Supplementary Table S2) were 5′ 32P-end labeled with 0.3 mCi of γ-32P ATP (Perkin Elmer) by incubating with 10 U of T4 polynucleotide kinase (New England Biolabs ...
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bioRxiv - Biophysics 2021Quote: ... Validation assays for 5-HT5AR-Gi1 expression were performed as described above using 10nM [3H]-LSD (82.4 Ci/mmol, PerkinElmer, Waltham, MA).
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bioRxiv - Cancer Biology 2020Quote: ... Probes were prepared by random priming of corresponding PCR products (5’ and GFP templates) in the presence of [αP32]CTP (PerkinElmer BLU513H100UC). Unincorporated nucleotides were removed using a Sephadex G-50 column (GE Healthcare 28-9034-08) ...
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bioRxiv - Neuroscience 2020Quote: ... the solvent was evaporated under N2 flow and resuspended with 500 mL of chloroform/methanol (2:1v/ v) and half the suspension was placed separately into vials with 5 mL of scintillation solution (Ultima Gold XR, Perkin Elmer). Levels of radioactivity were measured on the Liquid Scintillation Analyzer ...
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bioRxiv - Microbiology 2021Quote: ... Aliquots of 100 μL were transferred daily (up to 10 days post dormancy induction) to a 96 well plate where 5 μCi 3H-hypoxanthine (Perkin Elmer), 5 μCi 3H-adenosine (Perkin Elmer) ...
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bioRxiv - Cell Biology 2020Quote: ... Glass fibre prefilters were air dried for 5 min and the amount of 35S-labeled methionine was analyzed with 5 ml liquid scintillation cocktail Ultima Gold™ (6013329, PerkinElmer) by Liquid Scintilation Analyzer Tri-Carb 4910 TR (PerkinElmer).
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bioRxiv - Genomics 2020Quote: ... washing (3 times 5 min in TNT) and 8 min incubation using Fluorescein Tyramide (for pimonidazole NEL701A001KT, perkin Elmer) or Cy3 (NEL704A001KT, Perkin Elmer) 1:50 in amplification diluent.
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bioRxiv - Cell Biology 2022Quote: ... An equal volume of cell culture media and esterase substrate (100 μM in esterase assay media at pH 5) was transferred to a black walled clear bottomed plate (Perkin Elmer) and fluorescence was measured after 1 h.
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bioRxiv - Microbiology 2020Quote: ... cooled to room temp (5 min) and absorbance was read at 432 nm on a Wallac 1420 VICTOR Multilabel reader (Perkin Elmer). Formaldehyde standards were prepared daily from 1 M formaldehyde stock solutions and a standard curve was alongside all sample measurements.
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bioRxiv - Bioengineering 2020Quote: ... Lungs were incubated for 5 minutes and then placed onto black paper and imaged with an IVIS Spectrum CT (Perkin Elmer). Lung luminescence was then quantified using Living Image software (Perkin Elmer).
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bioRxiv - Plant Biology 2020Quote: ... was incubated with oligonucleotide probes (0.01 pmol-10 fmol) 5’-radiolabeled with [y-32P]ATP (5000 Ci/mmol; PerkinElmer Life Science). Different dsDNA structures were prepared by annealing the radiolabeled sense oligonucleotides with a twofold excess of unlabeled complementary oligonucleotide and purified on non-denaturing polyacrylamide gels ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were pre-treated with anisomycin (100 ug/ml) for 5 min to inhibit cytoplasmic translation then pulsed with 200 uCi/ml 35S Met-Cys (EasyTag-Perkin Elmer). In chase experiments ...
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bioRxiv - Biochemistry 2021Quote: ... Thirty μL of tissue homogenate supernatant were mixed with 370 μL of the oxidation reaction mixture containing 7% BSA/5 mM palmitate/0.01 μCi/μL 14C-palmitate (PerkinElmer, Waltham, MA) and incubated at 37 °C for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... cooled to room temp (5 min) and absorbance was read at 432 nm on a Wallac 1420 VICTOR Multilabel reader (Perkin Elmer). Formaldehyde standards were prepared daily from 1 M formaldehyde stock solutions and a standard curve was generated in parallel with samples at each measurement (timepoint).
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bioRxiv - Biochemistry 2021Quote: ... cells were starved for cysteine and methionine for 15-30 min and pulse labeled for 5 min with 55 µCi/ 35-mm dish of Easytag express 35S protein labeling mix (Perkin Elmer). Where indicated (+DTT) ...
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bioRxiv - Microbiology 2020Quote: ... per well and gently mixing by manually swirling the plate (a 5 min incubation prior to reading the plate is recommended) before reading luciferase activity on an Envision plate reader (Perkin Elmer). Due to time sensitivity of the luciferase signal ...