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Citations for PerkinElmer :
1151 - 1200 of 1217 citations for Cow Eukaryotic Translation Initiation Factor 1 EIF1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... the remaining 20% of the beads (supplemented with 40 µl of 2X ROTI®Load and boiled at 95°C for 10 min) were subjected to SDS-PAGE for immunoblotting together with 1% input samples using HRP-streptavidin conjugate (PerkinElmer; #NEL750001EA) or anti-p65 (Santa Cruz Biotechnology ...
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bioRxiv - Molecular Biology 2023Quote: ... Fluorescently labeled RNA was obtained by replacing 1/4 volume of CTP with Cyanine 3-CTP (Cy3-CTP) or Cyanine 5-CTP (Cy5-CTP) (PerkinElmer NEL581001EA) in the in vitro transcription reaction.
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bioRxiv - Neuroscience 2024Quote: ... washed one time with PBS and one time with PBS+DAPI (1:10 000) and subsequently imaged on the Opera Phenix High Content Screening System (Perkin Elmer) on a 40X water objective ...
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bioRxiv - Bioengineering 2023Quote: UV-vis spectra of the medium and hydrogel samples were measured using a plastic cuvette (1 cm light path) with a UV-visible light spectrophotometer (Lambda 35, Perkin Elmer). The transmittance was measured over the range of 800-400 nm.
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bioRxiv - Cell Biology 2024Quote: ... After two washes of 5min in 1X RNAscope wash buffer slides were incubated with 1:1500 Cyanine 3 amplification reagent (PerkinElmer, #FP1170012UG), or 1:1000 Cyanine 5 amplification reagent (PerkinElmer ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were pulse labeled for 1 hr with 120 μCi of EasyTag Express35S Protein Labeling Mix [35S]-Cys/Met (PerkinElmer; Waltham, MA). Immediately after the radioactive pulse ...
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bioRxiv - Developmental Biology 2021Quote: ... M13mp18 ssDNA was added as a template for DNA replication at the indicated concentrations in presence of α-;32PdCTP (3000Ci mmol−1, Perkin Elmer). At the indicated time points half of the samples were neutralized in 10 mM EDTA ...
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bioRxiv - Neuroscience 2019Quote: ... Confluent cultures were changed by quiescent medium for 24 h before they were labelled for 4 h with 1 μCi/ml [3H] arachidonic acid (PerkinElmer Life Sciences). Washing cells once with PBS containing 0.1% free fatty acid albumin and twice with PBS alone ...
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bioRxiv - Cancer Biology 2020Quote: ... proteins were first labeled for 48 h with 0.25 μM Ci ml-1 L-[U-14C] VALINE (Perkin Elmer, Waltham, MA, USA) in GIBCO-RPMI 1640 medium (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were fixed with 2% PFA at RT for 30 min and stained with the Hoechst 33342 dye (1 μg/ml) at 37 °C and 5% CO2 before imaging with the Operetta™ high content imaging system (PerkinElmer Inc.). Forty fields were acquired from each well using appropriate filter sets ...
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bioRxiv - Developmental Biology 2022Quote: ... Tyramide signal amplification reaction was performed using Cy3 fluorescent dye (diluted 1:50 using amplification diluent) (Perkin Elmer Life Sciences, Inc., NEL753001kt). The embryos were co-stained with CrebA and Crb antibodies for visualizing SG nuclei and apical cell boundaries ...
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bioRxiv - Immunology 2021Quote: ... Virus-mAb/plasma-containing media was then aspirated from cells and 100 μL of a 1:2 dilution of SteadyLite Plus (Perkin Elmer, 6066759) in PBS with Ca++ and Mg++ was added to cells ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated in channel 1-specific HRP (323104) for 15min and Cy3-TSAP (1.5K, tyramine signal amplification plus, NEL744E001KT; PerkinElmer Waltham, MA, USA) for 30 min followed by a 15 min incubation in HRP blocker (323107) ...
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bioRxiv - Cell Biology 2021Quote: ... media was removed and replaced with serum-free Modified Eagles Medium (MEM; Mediatech) containing 1 µCi/ml of 3H-myo-inositol (PerkinElmer Life Sciences). Forty-eight hrs post-infection ...
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bioRxiv - Biochemistry 2019Quote: ... the reaction mixtures in Flash plates were incubated for 1 hour and the CPM were measured using Topcount plate reader (Perkin Elmer, www.perkinelmer.com). The CPM counts in the absence of compound for each data set were defined as 100% activity ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Lipodisqs labelled with Cy5 either bound to the polymer SMA or to the lipid DLPE (Supplementary Table 1) were used to study the biodistribution in healthy mice using an IVIS® Spectrum in vivo imaging system (Perkin Elmer). Mice were intravenously injected with these two formulations of Lipodisqs (injected volume of 100 μl with a dose of 100 mg/kg lipid ...
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bioRxiv - Pathology 2021Quote: ... incubated for 1 hour and imaged for brightfield and Hoechst staining using the Operetta CLS™ High-Content Analysis System (PerkinElmer, Inc.). Stained nuclei were counted using the Harmony® software (PerkinElmer ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 3-plex positive control probe or with a cocktail of Abcb5-C1 and Abcb4-C2 probes with a 1:750 dilution of TSA-Fluorescein Plus and TSA-Cyanine 3 Plus (PerkinElmer, Shelton, CT), respectively ...
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bioRxiv - Immunology 2021Quote: ... Virus-mAb/plasma-containing media was then aspirated from cells and 100 μL of a 1:2 dilution of SteadyLite Plus (Perkin Elmer, 6066759) in PBS with Ca++ and Mg++ was added to cells ...
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bioRxiv - Neuroscience 2019Quote: ... The secondary and Opal™ steps were then repeated using the anti-rabbit secondary and Opal™ 650 reagent (1:1000; PerkinElmer). Sections were then washed 3x for 2 min each in TBS-T and counterstained with DAPI to label nuclei.
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bioRxiv - Microbiology 2021Quote: ... Virus-mAb-containing media was then aspirated from cells and 50 μL of a 1:2 dilution of SteadyLite Plus (Perkin Elmer, 6066759) in PBS with Ca++ and Mg++ was added to cells ...
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bioRxiv - Biophysics 2021Quote: ... 50 nM SpNatC (WT or mutants) was mixed with 50 μM Ac-CoA (14C-labeled, 4 mCi mmol-1; PerkinElmer Life Sciences) and varying peptide concentrations (ranging from 1.95 μM to 500 μM ...
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bioRxiv - Bioengineering 2022Quote: ... permeabilized for 30 min in 0.5% Triton X-100 in PBS and stained overnight with 1 μmol/mL CNA35-OG488 [30] and 0.2 nmol/ml OsteoSenseTM 680 (NEV10020EX, PerkinElmer, Waltham, MA, USA) at 4 ºC to visualize collagen and hydroxyapatite ...
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bioRxiv - Biochemistry 2022Quote: ... Detection reagents were added stepwise with 30 min incubation of anti-H3K4me3 antibody and Ulight-Streptavidin with reaction time points followed by 1 hr incubation with Europium anti-rabbit antibody in 384 well white microplates (PerkinElmer OptiPlate-384). TR-FRET emission at 665 nm and at 615 nm by 320 nm excitation with 50 μs delay and 100 μs integration time was measured using a Molecular Devices SpectraMax M5e plate reader ...
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bioRxiv - Microbiology 2023Quote: Prokaryotic (bacteria and archaea) heterotrophic production was estimated using the 3H-leucine incorporation method (Perkin Elmer, specific activity 100 Ci mmol−1). Water samples (1.7 mL each ...
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bioRxiv - Biochemistry 2023Quote: ... Reactions were initiated by adding an equal volume of assay buffer containing 1 μm [γ-32P]GTP (~50 cpm/fmol, Perkin Elmer) at 30 °C ...
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bioRxiv - Biochemistry 2023Quote: ... BRB80 was mixed with a 1/10 volume of 10X nucleotide exchange buffer (10 mM GTP, 30 nM GTP α-32P (PerkinElmer BLU506H250UC), 1 mM nocodazole ...
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bioRxiv - Cell Biology 2023Quote: ... 5 μl cAMP Eu-cryptate reagent and 5 μl of anti-cAMP-d2 working solution were added to the 384-well plates and incubated for 1 h.54 Fluorescence signals were detected at 620/665 nm using the Multimode Plate Reader (PerkinElmer EnVision 2105).55 Data were analyzed with GraphPad Prism 9.0 ...
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bioRxiv - Molecular Biology 2023Quote: ... GLuc production was measured as a surrogate for IFN-β promoter activation by adding 25 µl coelenterazine-solution (1 µg/ml in H2O) was to 25 µl supernatant.reader (Perkin Elmer, Waltham, USA) and luminescence was measured ...
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bioRxiv - Cancer Biology 2023Quote: ... The amount of eATP was analyzed according to the protocol described by the manufacturer of ATPlite 1-step Luminescence Assay System (PerkinElmer, Cat# 6016736) either at 0 hours (eATP concentrations were determined immediately ...
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bioRxiv - Cancer Biology 2024Quote: ... 1:3 dilution steps) using a Janus Gripper with 384-channel Modular Dispense Technology dispensing head (revvity, previously Perkin Elmer, MA, USA). Afterwards ...
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Wnt-dependent activation of ERK mediates repression of chondrocyte fate during calvarial developmentbioRxiv - Developmental Biology 2021Quote: ... The slides were then washed in 1X TBS-T and incubated in HRP-conjugated goat-anti-rabbit secondary (1:1500, PerkinElmer NEF812001EA, Akoya Biosciences) for 45 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Luminescence was measured at 1 h post PathHunter© detection reagent addition using Victor3 plate reader (Perkin Elmer 0.5-s/well integration time). Data were normalized to control treated with the highest concentration of ligand (100%) ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were stained with Hoechst (1/5000) for 30 min and fluorescence was monitored using victor x4 2030 Multilabel Reader (PerkinElmer, Waltham, MA, USA) with excitation wavelength at 350 nm ...
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bioRxiv - Immunology 2021Quote: ... were plated in 3 ml of complete medium supplemented with IL-2 (20 IU/ml) and incubated with 1 μl of 3H-labeled ROL (PerkinElmer, Boston, MA, USA) for 4 h ...
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bioRxiv - Bioengineering 2020Quote: ... cells were resuspended in 100 μL of medium at a 4:2:1:1:1 ratio and transferred to ultralow attachment (ULA) round bottom 96-well plates (CellCarrier Spheroid ULA 96-well Microplates, PerkinElmer, Waltham, MA, USA). In another method ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... All the videos (2 min, 4 frames s-1) were acquired at 37 °C using a Nikon TIRF microscopy system (Perkin Elmer, Waltham, MA) on an inverted Ti microscope equipped with a 100× objective and an ImageEM C9100-13 camera (Hamamatsu Photonics ...
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bioRxiv - Cell Biology 2021Quote: ... sections were washed in TBST and incubated with Alexa Fluor® 594 AffiniPure Goat Anti-Rabbit IgG (H+L) (Abacus DX, Australia) with Spectral DAPI (FP1490) added (1/1000) (Perkin Elmer, USA) for one hour at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... Organoid/hydrogel cultures were serum starved overnight before treatment with EGF (20 ng/mL) and promegestone/R5020 (1, 10, 100 nM; Perkin Elmer, Cat. #: NLP00400) and 10 nM R5020 with or without the addition of FAK (1 μM ...
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bioRxiv - Immunology 2020Quote: ... The IgGs and ACE2 were incubated in the plates for 1 hour and ACE2-Fc-Biotin was detected by DELFIA Eu-N1 Streptavidin (Perkin Elmer, 1244-360) using the DELFIA-TRF system (Perkin Elmer).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Oxaliplatin loaded Cedrus libani Sporopollenin (CD-SP-OX) were analyzed in the range of 4000-650 cm-1 using an FT-IR spectrometer (Perkin Elmer 100, USA) under ambient conditions.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... lung tissues harvested from euthanized mice were soaked for 10 min in pre-warmed (37°C) PBS with 2% FBS and a 1:10 dilution of Xenolight RediJect Coelenterazine h (Cat# 706506, Perkin Elmer, Waltham, MA). Following a 12-15 min incubation ...
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bioRxiv - Cell Biology 2020Quote: In vitro kinase reactions were developed in a final volume of 50 μl containing 0.02 mM [γ-32P]ATP (1 µCi per tube, Perkin Elmer, Massachusetts, USA), 50 mM Tris-HCl ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were washed again in PBS with 0.1% Tween-20 six times for 1 hour and incubated in fluorescein tyramide reagent (Perkin Elmer, Cat. No. SAT701001EA) diluted 1:70 in amplification diluent for 50 minutes at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... They were stored in PBS + 0.1% w/v sodium azide before imaging on a X-ray micro-CT device (Quantum FX Caliper, Life Sciences, Perkin Elmer, Waltham, MA) hosted by the PIV Platform ...
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bioRxiv - Cell Biology 2023Quote: All the videos (2 min, 4-5 frames s-1) were acquired at 37°C using a Nikon TIRF microscopy system (Perkin Elmer, Waltham, MA) on an inverted Ti microscope equipped with a 100× objective and an ImageEM C9100-13 camera (Hamamatsu Photonics ...
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bioRxiv - Microbiology 2024Quote: ... pseudoviruses were added in quadruplicate on HEK293T-ACE2 target cells which were seeded at a density of 1 × 104 cells/well in 96-well luminometer-compatible tissue culture plates (PerkinElmer, Waltham, MA, USA) 24h before infection ...
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bioRxiv - Cancer Biology 2024Quote: ... then separated in an 1% formaldehyde-containing agarose gel and transferred onto a GeneScreen Plus hybridization transfer membrane (Perkin Elmer, Waltham, MA, #NEF987001PK). RNAs on the membrane were crosslinked by exposing to UV light ...
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bioRxiv - Immunology 2021Quote: ... Plasma 2-[1-14C]-Deoxy-D-glucose radioactivity was directly measured in a liquid scintillation counter (Tri-Carb 2810 TR, Perkin Elmer, Waltham, MA, USA). Tissue lysates were processed through ion exchange chromatography columns (Bio Rad Laboratories Inc. ...
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bioRxiv - Microbiology 2021Quote: ... in a ratio of 5:95 and analyzed at the mid-IR region (400-4,000 cm-1) of the spectrometer (Perkin Elmer Spectrum 100, United States). The biodegraded products of AB1 were identified using LC-MS analyses (Dionex Ultimate 3000 ...