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Citations for PerkinElmer :
1101 - 1150 of 1636 citations for 6 Methyl 5 4 phenyl 1 3 thiazol 2 yl 2 trifluoromethyl nicotinic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... TZM-bl target cells were seeded at a density of 5 × 103 cells/well in 96-well luminometer-compatible tissue culture plates (Perkin Elmer) 24 h before infection ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mL fractions containing soluble inositol phosphates were mixed with 3 mL scintillation cocktail (Ultima-Flo AP) and counted for 5 min in a liquid scintillation counter (Tri-Carb 2910 TR, Perkin Elmer). The lipid inositol extract was counted in a liquid scintillation counter ...
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bioRxiv - Biochemistry 2023Quote: ... Each binding reaction was allowed to reach equilibrium at 30 °C for 5 min prior to fluorescence polarization measurements in a Victor X5 Multilabel Plate Reader (Perkin Elmer). Each Kd measurement was calculated according to our previous work (45) ...
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bioRxiv - Developmental Biology 2021Quote: ... Opal570 (1:500) and Opal650 (1:1500) using the Opal 7 Kit (NEL797001KT; PerkinElmer).
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bioRxiv - Developmental Biology 2021Quote: ... The reaction with tyramide-Cy3 (1:50 dilution in 1 ×Plus Amplification Diluent (PerkinElmer), followed by 1:100 dilution with DW ...
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bioRxiv - Genomics 2020Quote: ... Prox-1 probes were visualized by Opal 650 reagents (1:1500 dilution, Perkin Elmer). Slides were additionally co-stained with rabbit anti-human vWF polyclonal antibody (Dako ...
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bioRxiv - Immunology 2021Quote: ... 1 μCi α32P-ATP (PerkinElmer)) for 90 min at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... and loaded overnight with MEM +HIHS cell culture media supplemented with 4 μCi/mL D-[2,3-3H]aspartate (Perkin Elmer, Waltham, MA, cat. #NET501001MC). Coverslips were washed in isosmotic Basal medium containing (in mM ...
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bioRxiv - Biochemistry 2021Quote: ... an optimized set of 4 small interfering RNAs (siRNAs; SMARTPool) targeted against human Furin were purchased from Horizon Discoveries (Perkin Elmer, Lafayette, LA, USA) and transfections of HeLa cells were carried out using INTERFERin (PolyPlus ...
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bioRxiv - Cancer Biology 2021Quote: ... For multiplexed immunohistochemistry signals were detected using Opal™ 4-Plex Kit according to the manufacturer’s protocol (catalog no. NEL810001KT; Perkin Elmer, Waltham, MA, USA). For more details see Supplemental Materials section.
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bioRxiv - Microbiology 2020Quote: Purified Caulobacter ParB-His6 or TetR-His6 (final concentration: 25 μM) were incubated with 5 nM radiolabeled P32-α-CTP (Perkin Elmer), 30 μM of unlabeled cold CTP (Thermo Fisher) ...
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bioRxiv - Neuroscience 2020Quote: ... The receptor autoradiography procedure was performed using a linear V1a-R antagonist [125I]-d(CH2)5(Tyr[Me])-AVP (Perkin Elmer, USA) or a linear OXTR antagonist [125I]-d(CH2)5[Tyr(Me)2-Tyr-Nh2]9-OVT (Perkin Elmer ...
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bioRxiv - Plant Biology 2021Quote: ... Seedlings were metabolically labelled with radioactive phosphate by incubating them overnight for ~16-20 hrs with 10 μL (5-10 μCi) carrier-free 32P-PO43- (32Pi; PerkinElmer, The Netherlands) in labelling buffer ...
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bioRxiv - Molecular Biology 2020Quote: 100 pmol of WT or mutant RNA oligonucleotides (synthesized by Integrated DNA Technologies, Inc.) were 5’- end labeled with [γ32P] ATP (Perkin Elmer, NEG035C001MC) and T4 polynucleotide kinase (New England Biolabs ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were finally washed three times with PBST for 5 min and protein levels were visualized by enhanced chemiluminescence (Perkin Elmer, NEL104001EA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Tumors were isolated from mice and sectioned into 5 micrometer sections for staining with the desired markers (below) using Tyramide Signal Amplification (PerkinElmer, Waltham MA) per manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2021Quote: ... The colonies were grown in 160 μL of TAP medium for 5 days in black clear bottom 96 well plates (CellCarrier-96 Black, 6005550, PerkinElmer Inc, USA), sealed with a paper tape ...
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bioRxiv - Developmental Biology 2024Quote: ... Plates (with lid on) were placed in the Opera Phenix High-Content Screening System (Perkin Elmer; prewarmed to 37C and 5% CO2). Images were collected every 30 min for 24 hours.
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bioRxiv - Neuroscience 2023Quote: ... produced in Sf9 cells, and ROCK1 (Carna Biosciences, Natick, MA; 5 nM) using the Lance-FRET kinase assay system (PerkinElmer, Waltham, MA). Other human recombinant PKCs (SignalChem Biotech ...
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bioRxiv - Immunology 2023Quote: ... Hybridization signals were detected by fluorescent signal using peroxidase-based Tyramide Signal Amplification-Plus Fluorescein or TSA Plus Cyanine 5 (Perkinelmer, Shelton, CT). Finally ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Determination of Gαi and Gαs selective signaling at the 5-HT1AR was performed applying an IP accumulation assay (IP-One HTRF®, PerkinElmer, Rodgau, Germany) according to the manufacturer’s protocol and in analogy to previously described protocols (15 ...
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bioRxiv - Immunology 2023Quote: ... Fluorescent signals were monitored in real-time with a microplate reader for 24 h within a tissue culture incubator (37 °C, 5 % CO2) on black OptiPlate 96-well (PerkinElmer, MA, USA) under 20x objective ...
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bioRxiv - Systems Biology 2024Quote: ... The NPCs were cultured in a 12-well dish to confluency in NPC media and then dissociated using Accutase for 5 minutes at 37°C to single cell resolution and plated on 5 µg/mL human laminin and 5µg/mL fibronectin-coated 384 well plates (Perkin Elmer Cat #6057308) at 12,000 cells/well in NPC media and 10 µM ROCKi ...
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bioRxiv - Immunology 2024Quote: ... Monocytes from five healthy donors were resuspended in an RPMI medium at 5 x 105 c/ml and distributed into the wells (100 μL) of white opaque 96-well microplates (Perkin Elmer, PKE_6005299). Macrophage cell cultures were generated by adherence of monocytes to cell culture plasticware as previously described (16) ...
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bioRxiv - Cancer Biology 2024Quote: ... solution (5 µg/l) was selected as the internal standard and added online during the ICP-MS analysis (NexION 2000, Perkin Elmer, USA). The method detection limits were also checked periodically ...
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bioRxiv - Biochemistry 2024Quote: ... Hairpin substrates with a two-nucleotide 5′-overhang end for EM-SAs were assembled by self-annealing oligonucleotides that were 5′-end labeled with [γ-32P]-ATP (Perkin Elmer) and T4 polynucleotide kinase (NEB) ...
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bioRxiv - Developmental Biology 2021Quote: ... Opal570 (1:500) and Opal650 (1:1500) using the Opal 7 Kit (NEL797001KT; Perkin Elmer).
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bioRxiv - Immunology 2021Quote: ... The NIT-1 cells were then labeled with 1 μCi/well of 51Cr (Perkin Elmer) for 3 hours at 37°C and co-cultured with AI4 T cells at a E:T ratio of 20:1 for 16 hours ...
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bioRxiv - Developmental Biology 2020Quote: ... Opal570 (1:500) and Opal650 (1:1500) using the Opal 7 Kit (NEL797001KT; Perkin Elmer).
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated with a D1R antagonist for 1h at 4°C in a solution equivalent to the pre-incubation buffer with the addition of 1nM [3H]SCH23390 (Perkin Elmer, USA, 85 Ci/mmol). Non-specific binding was determined by incubating sections under the same conditions with excess amount of the D1R ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated with a D2R antagonist for 1h at 4°C in a solution equivalent to the pre-incubation buffer with the addition of 4nM [3H]raclopride (Perkin Elmer, USA, 60.1 Ci/mmol). Non-specific binding was determined by incubating sections under the same conditions with excess amount of the D2R ...
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bioRxiv - Evolutionary Biology 2021Quote: ... biotin-labelled probes were visualized by streptavidin-horseradish peroxidase (HRP) conjugates with Cyanine 5 Plus Amplification Reagent in a TSA kit as the substrate (PerkinElmer, Boston, MA, USA). Sense probes labelled with DIG and biotin by SP6 RNA transcription kit were used as control ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 80-120 mg/kg body weight) / xylazine (Xy, 5-10 mg/kg body weight) cocktail) using an IVIS Spectrum In Vivo Imaging System (Perkin Elmer, Rodgau, Germany) with images acquired using the Living Image® software package (Perkin Elmer).
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bioRxiv - Microbiology 2021Quote: ... The washed filters were dried for 30 min under a heat-lamp and placed in a 20-ml Borosilicate Glass Scintillation vial containing 5 ml of Ultima Gold F liquid scintillation cocktail (Perkin Elmer, MA, USA). 14C radioactivity retained on the filters was quantified using an LS6500 scintillation system (Beckman Coulter Inc. ...
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bioRxiv - Bioengineering 2022Quote: Cu and Zn ion release from PCu coupons (surface area 5 cm2) and Cu-coated SS coupons was measured using inductively coupled plasma optical emission spectrometry (ICP-OES, Perkin Elmer, Optima 8300). The PCu coupons were wet ground with a 600 grit SiC emery paper and rinsed with DI water ...
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bioRxiv - Neuroscience 2022Quote: ... or 1 μg of GST-Raf13 as the Pak1 substrate with a total volume of 5 μL in a 384-well plate (PerkinElmer, Waltham, MA, USA) at 30°C for 1 h ...
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bioRxiv - Cell Biology 2022Quote: ... Bioluminescence imaging was performed at days 5 and 7 post engraftment using an IVIS Lumina III in vivo imaging system (Perkin Elmer, Waltham, MA). IVIS instrument exposure time was 3 seconds ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... 5-HT uptake was initiated by the addition of 10 nM [3H] 5-HT (Cat No: NET498001MC; 24.82 Ci/mmol: Perkin Elmer, Santa Clara, CA). and the uptake was terminated at 5 min with the addition of fluoxetine followed by rapid filtration over GF-B filters ...
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bioRxiv - Plant Biology 2021Quote: ... 10 µM cycloheximide and 10 µCi ml-1 Na-14C acetate (PerkinElmer: 56.6 mCi mM-1) were then added to the culture ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: [3H]-DHA (1-[4,6-propyl-3H]dihydroalprenolol, specific activity 91 Ci·mmol-1) was obtained from PerkinElmer Life and Analytical Sciences (Beaconsfield ...
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bioRxiv - Microbiology 2021Quote: ... spiked with 500 nmol 3H-leucine L-1 (Perkin Elmer, specific activity 100 Ci mmol- 1) and incubated in the dark for 4 h ...
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bioRxiv - Plant Biology 2024Quote: ... 10 µM cycloheximide and 10 µCi mL-1 Na-14C acetate (PerkinElmer: 56.6 mCi mM-1) were then added to the culture for the 7-min pulse ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and ATPlite 1 Step (Perkin Elmer). The main instruments used were the microplate reader SpectraMax 340PC (Molecular Device ...
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bioRxiv - Developmental Biology 2022Quote: ... Opal 650 (1:1500, PerkinElmer, FP1496001KT), Opal 690 (1:1500 ...
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bioRxiv - Developmental Biology 2022Quote: ... Opal 690 (1:1500, PerkinElmer, FP1497001KT). Following signal amplification of the target probes ...
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bioRxiv - Cell Biology 2022Quote: ... 1:750 TSA Cy3 (#NEL704A001KT, PerkinElmer) and TSA Cy5 (#NEL75A001KT ...
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bioRxiv - Neuroscience 2021Quote: ... Opal 570 (1:1500, Perkin Elmer), and Opal 690 (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... 1 μCi [14C]glycerol (Perkin Elmer) + 50 μM glycerol to stimulate glycerolipid synthesis ...
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bioRxiv - Biochemistry 2024Quote: ... 1 μL γ-32P-ATP (PerkinElmer) and 10 U T4 polynucleotide kinase (New England Biolabs ...
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bioRxiv - Biophysics 2023Quote: ... 1 μM 3H-L-Asp (PerkinElmer), and 0.5 μM valinomycin ...