Labshake search
Citations for PerkinElmer :
1101 - 1150 of 1343 citations for 4' Trifluoromethoxy 5 trifluoromethyl 1 1' biphenyl 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... lung tissues harvested from euthanized mice were soaked for 10 min in pre-warmed (37°C) PBS with 2% FBS and a 1:10 dilution of Xenolight RediJect Coelenterazine h (Cat# 706506, Perkin Elmer, Waltham, MA). Following a 12-15 min incubation ...
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bioRxiv - Developmental Biology 2020Quote: ... we followed the protocol provided by the commercial company except that all samples were stained in 1 to 80 dilution of Cy3-conjugated or FLU-conjugated Tyrimide substrates for 60 minutes (Perkin Elmer NEL753001 Kit).
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bioRxiv - Cell Biology 2020Quote: In vitro kinase reactions were developed in a final volume of 50 μl containing 0.02 mM [γ-32P]ATP (1 µCi per tube, Perkin Elmer, Massachusetts, USA), 50 mM Tris-HCl ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were washed again in PBS with 0.1% Tween-20 six times for 1 hour and incubated in fluorescein tyramide reagent (Perkin Elmer, Cat. No. SAT701001EA) diluted 1:70 in amplification diluent for 50 minutes at room temperature ...
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bioRxiv - Genomics 2024Quote: ... size-selected sample was reamplified with 25 μL of NEXTFLEX PCR Master Mix 2.0 and 2 μL of 1:10 diluted NEXTFLEX Primer Mix 2.0 as described in the NEXTFLEX Cell free DNA-Seq Kit (V2) (Perkin Elmer, Pittsburgh, Pennsylvania, USA), step C PCR amplification ...
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bioRxiv - Microbiology 2024Quote: ... pseudoviruses were added in quadruplicate on HEK293T-ACE2 target cells which were seeded at a density of 1 × 104 cells/well in 96-well luminometer-compatible tissue culture plates (PerkinElmer, Waltham, MA, USA) 24h before infection ...
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bioRxiv - Developmental Biology 2022Quote: ... They were stored in PBS + 0.1% w/v sodium azide before imaging on a X-ray micro-CT device (Quantum FX Caliper, Life Sciences, Perkin Elmer, Waltham, MA) hosted by the PIV Platform ...
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bioRxiv - Biochemistry 2024Quote: ... The lysed cells were diluted 1:2 with assay buffer after which 10 μL/well was pipetted to a white 384-well OptiPlate (PerkinElmer, Waltham, MA, USA). Next ...
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bioRxiv - Bioengineering 2024Quote: ... MDA-MB-231 cells expressing HMGB1-GFP and CRT-RFP (stained MDA cells) were cultured in 100 µl of RPMI medium (10% FBS and 1% penicillin) in 96-well plate (PerkinElmer ViewPlate – 96 TC) at the density of 5 × 103 cells/well ...
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bioRxiv - Evolutionary Biology 2021Quote: ... equipped with an Elite-5 column (30 m × 0.25 mm× 0.25 µm, Perkin Elmer, Shelton, CT, USA). The effluent from the column was split into two equal parts ...
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bioRxiv - Molecular Biology 2020Quote: ... The 5’FAM donor fluorescence was recorded by a SPAD (SPCM-AQR-14, PerkinElmer Optoelectronics, Quebec, Canada), through a 513/17 band pass emission filter (Omega ...
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bioRxiv - Molecular Biology 2021Quote: ... HeLa cells (5×104 cells/ml) were adhered in an Cellcarrier Ultra optical 96-well plate (PerkinElmer). Cells were washed three times with ice-cold PBS and fixed in 4% (vol/vol ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 × 102 cells were seeded on cell culture-coated 96-well white-walled CulturePlates (Perkin Elmer #6005688). Cells were lysed with CellTiter-Glo® 2.0 Reagent (Promega) ...
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bioRxiv - Biochemistry 2021Quote: ... 100 pmol of RNA were 5’-end labeled with [γ- 32P]-ATP (3000 Ci/mmol, Perkin Elmer) using T4 polynucleotide kinase (NEB ...
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bioRxiv - Plant Biology 2020Quote: ... Images of dichlorofluorescein (DCF) fluorescence were acquired using an IVIS Lumina 5 apparatus (PerkinElmer, Waltham, MA, USA) for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... The signal was developed by incubating the slides with TSA plus Cyanine 5 system (PerkinElmer, Waltham, MA) for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... The tissue sections were stained with 5 μg/mL Pheno Vue Fluor 594-WGA (PerkinElmer Health Sciences), 2 μg/mL anti-E-cadherin ...
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bioRxiv - Molecular Biology 2023Quote: ... Tumors were observed weekly for 5 weeks using an IVIS Spectrum In Vivo Imaging System (PerkinElmer, USA) after i.p ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... [3H]5-HT (serotonin, 41.3 Ci/mmol) and [3H]citalopram (80 Ci/mmol) were purchased from PerkinElmer Life Sciences (Rodgau ...
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bioRxiv - Cell Biology 2022Quote: ... and cells were incubated for 5 min before addition of 200μCi 35S-labeled methionine/cysteine mixture (PerkinElmer) and incubation for 1 hr at 37°C ...
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bioRxiv - Plant Biology 2023Quote: ... resuspended in 2 N NaOH and mixed with 5 volumes scintillation fluid (Ultima Gold™ XR, PerkinElmer) for 14C-CPM counting in a TRI-CARB liquid scintillation counter (PerkinElmer ...
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bioRxiv - Synthetic Biology 2023Quote: ... Live animals (isoflurane sedated) were imaged every 5 min over 30 min by IVIS Spectrum (PerkinElmer, US) with an exposure time of 30 seconds ...
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bioRxiv - Biochemistry 2024Quote: ... Radiolabeling was performed on the 5’ end of oligonucleotides using [γ-32P] ATP ([6000μCi/mmol] (Perkin Elmer) and polynucleotide kinase (Roche Applied Science ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... a 5 to 10 mg sample was weighed precisely and placed in a ceramic sample pan (PerkinElmer). The analysis protocol involved loading the sample into the furnace ...
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bioRxiv - Genomics 2024Quote: ... A GS2 nick-translated probe was prepared using Texas Red-5- dCTP (PerkinElmer/Revvity, Boston, MA, USA) according to Kato et al.26 The telomeric probe ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 μL/well) was captured overnight at 4°C onto high-protein-binding ELISA plates (Spectraplate 96HB, Perkin Elmer). Plates were washed in PBS/Tween (0.05% v/v ...
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bioRxiv - Microbiology 2022Quote: ... one ml of culture was labelled for 4 min at 30 °C using 30 μCi γ-[32P]ATP (PerkinElmer). Samples were incubated with 6% sucrose ...
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bioRxiv - Immunology 2023Quote: ... uptake inhibition and degradation were determined in M[0] and M[IL-4] using 3H-choline chloride (Perkin Elmer) as previously described [4].
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 4 μL/well of cell culture supernatant was taken out and dispensed into OptiPlate-384 microplates (PerkinElmer, cat #: 6007290) that was pre-filled by 12 μL/well PBS by an EL406 ...
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bioRxiv - Cell Biology 2022Quote: Candidate small RNA sequences were identified from raw sequence data using cutadapt (ver. 2.8)21 with parameters “-u -4 -a A(10)” as recommended by PerkinElmer NextFlex Combo-Seq kit ...
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bioRxiv - Immunology 2024Quote: ... Clone 2C.4 was screened against human cardiac myosin and the β-adrenergic receptor (βAR) enriched membranes (Perkin Elmer) by ELISA ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by library preparation with the Next-Flex small RNA v.4 kit protocol (Perkin Elmer, NOVA-5132-06), in accordance to manufacture recommendations ...
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bioRxiv - Cell Biology 2020Quote: ... The blot was washed in TTBS for 3 times and the signal was developed using Western Lightening Plus-ECL kit (PerkinElmer, Waltham, MA) and detected on HyBlot CL X-ray films (Denville).
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bioRxiv - Molecular Biology 2021Quote: ... The plates were read at a wavelength of 460 nm using the VICTOR 3 V 1420 Multi-Label Microplate Reader (Perkin Elmer, USA). All reactions were carried out in triplicate using both antigenic peptides in separate experiments ...
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bioRxiv - Cell Biology 2021Quote: ... Imaging involving mNG-Bud8 (Figs. 3 & S2) was performed using a spinning disk confocal microscope (Ultra-VIEW VoX CSU-X1 system; Perkin Elmer-Cetus) equipped with a 100x /1.45 NA Plan-Apochromat Lambda oil immersion objective lens (Nikon) ...
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bioRxiv - Cell Biology 2021Quote: Time-lapse or live-cell fluorescence microscopy was performed using a Zeiss Axiovert 200 M microscope (Plan Apochromat 1006, 1.4NA objective) with an Ultra-View RS-3 spinning disk confocal system (PerkinElmer Inc., United States) which equipped with a CSU21 confocal optical scanner ...
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bioRxiv - Neuroscience 2021Quote: Live-cell imaging was performed at 37°C and with 3% CO2 using an Opera Phenix™ high content confocal microscopy system (Perkin Elmer). One hour prior to imaging ...
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bioRxiv - Microbiology 2021Quote: ... 200 μL of each phase were collected and measured in a 2480 automatic Gamma counter Wizard 2 3” (PerkinElmer, Waltham, MA, USA). LogD value were calculated using Excel by dividing measured values of octanol by water and logarithmize the result ...
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bioRxiv - Biophysics 2020Quote: ... HEK cell membrane proteins (100 μg/ml final concentration) were incubated with 3 nM [3H]muscimol (30 Ci/mmol; PerkinElmer Life Sciences), neurosteroid (3 nM–30 μM ...
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bioRxiv - Physiology 2024Quote: GFP fluorescence was monitored before and at several time points after a 3-week doxycycline treatment using an in vivo IVIS Spectrum Imaging System (PerkinElmer, Waltham, MA). Briefly ...
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bioRxiv - Immunology 2021Quote: ... Plasma 2-[1-14C]-Deoxy-D-glucose radioactivity was directly measured in a liquid scintillation counter (Tri-Carb 2810 TR, Perkin Elmer, Waltham, MA, USA). Tissue lysates were processed through ion exchange chromatography columns (Bio Rad Laboratories Inc. ...
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bioRxiv - Immunology 2021Quote: ... cells were pulse labelled with 50µCi of 35S-methionine and -cysteine for 1 min (EasyTag™ EXPRESS35S Protein Labeling Mix, Perkin Elmer, Waltham MA, USA) and immediately transferred into 10x volume of chase medium containing excess amounts of unlabelled cysteine (500 µg/ml) ...
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bioRxiv - Biochemistry 2021Quote: ... and 10 μL of 1/5000 dilution of Eu-W8044 labeled streptavidin and Biotin-cAMP in the Detection Buffer (PerkinElmer Life and Analytical Sciences) were added in reduced lighting conditions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and 10 μL of 1/5000 dilution of Eu-W8044 labeled streptavidin and Biotin-cAMP in the Detection Buffer (PerkinElmer Life and Analytical Sciences) were added in reduced lighting conditions ...
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bioRxiv - Immunology 2023Quote: ... Pelleted particles were resuspended in D10 medium and the concentration of HIV p24 was quantitated by using an HIV-1 p24 ELISA Kit (Perkin Elmer, Boston, MA, USA) [44 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: The level of phosphorylated extracellular signal-regulated protein kinase 1/2 (pERK1/2) was detected using the AlphaScreenTM SureFire Kit (PerkinElmer Life and Analytical Sciences). Briefly ...
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bioRxiv - Developmental Biology 2024Quote: ... Vectastain Elite ABC kit) followed by the tyramide signal amplification reaction (Cy3 fluorescent dye in amplification diluent; 1:50) (Perkin Elmer Life Sciences, NEL753001kt). To mark the SG and the apical membrane ...
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bioRxiv - Cancer Biology 2024Quote: ... The tumor burden was quantified weekly by bioluminescent signal intensity on isoflurane-anesthetized mice that received a subcutaneous injection of D-luciferin (150 μg luciferin/1 g mouse body weight) 10 min prior to IVIS® imaging (PerkinElmer, Waltham, MA,USA). Survival data were presented and reported in Kaplan–Meier plots.39
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bioRxiv - Cell Biology 2023Quote: ... Spectrum Spotlight 400 FT-IR Imaging system was used to acquire spatial resolution images in the mid-IR region from 4000 to 800LJcm−1 (Perkin Elmer, Waltham, MA, USA) of three consecutive sections/disc to minimize section-based variation ...
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bioRxiv - Cancer Biology 2022Quote: ... filters were incubated for 1 hour at room temperature with alkaline phosphatase-coupled goat antirabbit antibodies and developed with Western Lightning Plus (Perkin Elmer, Inc., Waltham, MA) according to manufacturer directions.