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Citations for PerkinElmer :
1101 - 1150 of 1167 citations for 1 2 Chloropyridin 3 yl 3 3 dimethylazetidin 2 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... was injected intraperitoneally per mouse 15 minutes prior to imaging for 1 minute exposure on In Vivo Imaging Spectrum Bioluminescent and Fluorescent Imaging Systems (IVIS) (Perkin Elmer) to confirm infection ...
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bioRxiv - Biochemistry 2023Quote: ... The amount of glycosylated Rac1 was determined by liquid scintillation counting of each sample for 1 min using a Tri-carb 2910TR scintillation counter (Perkin Elmer). The initial rates of the reaction were fit to the Michaelis Menten equation (eq 1 ...
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bioRxiv - Cell Biology 2023Quote: ... and 1% antibiotic/antimycotic at 37°C for 30 min and then labeled with Express [35S] Labeling Mix (PerkinElmer, NEG072-007) for 15 min ...
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bioRxiv - Genetics 2024Quote: ... was done for 1 hour at room temperature and following the addition of the western lightening ECL Pro ECL (Perkin Elmer), images were then directly recorded using Biorad Molecular Imager® Chemi DocTM XRS ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then solubilized with 200μL of 1% SDS 0.2N NaOH and transferred to a scintillation vial with 5mL of Insta-Gel Plus scintillation cocktail (Perkin Elmer #601339). Radioactivity was measured with the TopCount scintillation counter (Perkin Elmer) ...
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bioRxiv - Microbiology 2022Quote: ... in the ratio of 5:95 and analyzed at mid-IR region (400–4000 cm−1) by FTIR using Spectrum GX (PerkinElmer, USA).
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bioRxiv - Cancer Biology 2023Quote: ... Following an HRP conjugation step a TSA Opal 570 1:150 (Tyramide Signal Amplification Opal 4-Color Manual IHC Kit, Perkin Elmer) was linked to the IRF7 antibody previously bound to the slide overnight ...
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bioRxiv - Genomics 2023Quote: ... and streptavidin-conjugated Atto-425 (Bio Trend) at a concentration of 1:400 using TSA-biotin (TSA Plus Biotin Kit, Perkin Elmer). All nuclei were DAPI-stained ...
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bioRxiv - Plant Biology 2024Quote: ... khasiana peristome and lid EFNs (n = 1 each) were analyzed on a 2400 Series II CHNS Organic Elemental Analyzer (PerkinElmer, USA). Freeze-dried and crushed EFNs (5-10 mg ...
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bioRxiv - Plant Biology 2024Quote: ... khasiana peristome and lid EFNs (n = 1 each) were analyzed by Avio 200 Inductively Coupled Plasma Optical Emission Spectrometer (PerkinElmer, USA). Spectral range of the equipment was 165-900 nm with a resolution of < 0.009 nm at 200 nm ...
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bioRxiv - Molecular Biology 2024Quote: ... tRNA aminoacylation was performed in buffer A supplemented with 1 mM ATP in the presence of 20 µM [3H]-phenylalanine (specific activity 136 dpm/picomole, Perkin Elmer) and either purified E ...
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bioRxiv - Cancer Biology 2024Quote: ... The fluorescence intensity was measured after 1 h of incubation using an Envision 2104 Multilabel Plate Reader (PerkinElmer, Waltham, MA, USA) with an excitation wavelength of 380 ± 8 nm and an emission wavelength of 430 ± 8 nm.
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bioRxiv - Cell Biology 2020Quote: ... Cells were pulse labeled for 1 hr with 120 μCi of EasyTag Express35S Protein Labeling Mix [35S]-Cys/Met (PerkinElmer; Waltham, MA). Immediately after the radioactive pulse ...
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bioRxiv - Developmental Biology 2021Quote: ... M13mp18 ssDNA was added as a template for DNA replication at the indicated concentrations in presence of α-;32PdCTP (3000Ci mmol−1, Perkin Elmer). At the indicated time points half of the samples were neutralized in 10 mM EDTA ...
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bioRxiv - Cancer Biology 2020Quote: ... proteins were first labeled for 48 h with 0.25 μM Ci ml-1 L-[U-14C] VALINE (Perkin Elmer, Waltham, MA, USA) in GIBCO-RPMI 1640 medium (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were fixed with 2% PFA at RT for 30 min and stained with the Hoechst 33342 dye (1 μg/ml) at 37 °C and 5% CO2 before imaging with the Operetta™ high content imaging system (PerkinElmer Inc.). Forty fields were acquired from each well using appropriate filter sets ...
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bioRxiv - Developmental Biology 2022Quote: ... Tyramide signal amplification reaction was performed using Cy3 fluorescent dye (diluted 1:50 using amplification diluent) (Perkin Elmer Life Sciences, Inc., NEL753001kt). The embryos were co-stained with CrebA and Crb antibodies for visualizing SG nuclei and apical cell boundaries ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated in channel 1-specific HRP (323104) for 15min and Cy3-TSAP (1.5K, tyramine signal amplification plus, NEL744E001KT; PerkinElmer Waltham, MA, USA) for 30 min followed by a 15 min incubation in HRP blocker (323107) ...
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bioRxiv - Cell Biology 2021Quote: ... media was removed and replaced with serum-free Modified Eagles Medium (MEM; Mediatech) containing 1 µCi/ml of 3H-myo-inositol (PerkinElmer Life Sciences). Forty-eight hrs post-infection ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Lipodisqs labelled with Cy5 either bound to the polymer SMA or to the lipid DLPE (Supplementary Table 1) were used to study the biodistribution in healthy mice using an IVIS® Spectrum in vivo imaging system (Perkin Elmer). Mice were intravenously injected with these two formulations of Lipodisqs (injected volume of 100 μl with a dose of 100 mg/kg lipid ...
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bioRxiv - Pathology 2021Quote: ... incubated for 1 hour and imaged for brightfield and Hoechst staining using the Operetta CLS™ High-Content Analysis System (PerkinElmer, Inc.). Stained nuclei were counted using the Harmony® software (PerkinElmer ...
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bioRxiv - Biophysics 2021Quote: ... 50 nM SpNatC (WT or mutants) was mixed with 50 μM Ac-CoA (14C-labeled, 4 mCi mmol-1; PerkinElmer Life Sciences) and varying peptide concentrations (ranging from 1.95 μM to 500 μM ...
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bioRxiv - Immunology 2021Quote: ... HIV-1 p24 content (produced by the backbone vector) was quantified using the Alliance p24 ELISA Kit (PerkinElmer Health Sciences, Cat# NEK050B001KT). Relative luminescence units (RLUs ...
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bioRxiv - Microbiology 2023Quote: Prokaryotic (bacteria and archaea) heterotrophic production was estimated using the 3H-leucine incorporation method (Perkin Elmer, specific activity 100 Ci mmol−1). Water samples (1.7 mL each ...
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bioRxiv - Biochemistry 2022Quote: ... Detection reagents were added stepwise with 30 min incubation of anti-H3K4me3 antibody and Ulight-Streptavidin with reaction time points followed by 1 hr incubation with Europium anti-rabbit antibody in 384 well white microplates (PerkinElmer OptiPlate-384). TR-FRET emission at 665 nm and at 615 nm by 320 nm excitation with 50 μs delay and 100 μs integration time was measured using a Molecular Devices SpectraMax M5e plate reader ...
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bioRxiv - Cancer Biology 2023Quote: ... The amount of eATP was analyzed according to the protocol described by the manufacturer of ATPlite 1-step Luminescence Assay System (PerkinElmer, Cat# 6016736) either at 0 hours (eATP concentrations were determined immediately ...
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bioRxiv - Molecular Biology 2023Quote: ... GLuc production was measured as a surrogate for IFN-β promoter activation by adding 25 µl coelenterazine-solution (1 µg/ml in H2O) was to 25 µl supernatant.reader (Perkin Elmer, Waltham, USA) and luminescence was measured ...
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bioRxiv - Bioengineering 2022Quote: ... permeabilized for 30 min in 0.5% Triton X-100 in PBS and stained overnight with 1 μmol/mL CNA35-OG488 [30] and 0.2 nmol/ml OsteoSenseTM 680 (NEV10020EX, PerkinElmer, Waltham, MA, USA) at 4 ºC to visualize collagen and hydroxyapatite ...
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bioRxiv - Biochemistry 2023Quote: ... Reactions were initiated by adding an equal volume of assay buffer containing 1 μm [γ-32P]GTP (~50 cpm/fmol, Perkin Elmer) at 30 °C ...
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bioRxiv - Genetics 2022Quote: ... The physical titer of vector preparations was measured using the HIV-1 Gag p24 antigen immunocapture assay kit (PerkinElmer, Waltham, MA, USA) and expressed as p24 ng/ml ...
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bioRxiv - Cell Biology 2023Quote: ... 5 μl cAMP Eu-cryptate reagent and 5 μl of anti-cAMP-d2 working solution were added to the 384-well plates and incubated for 1 h.54 Fluorescence signals were detected at 620/665 nm using the Multimode Plate Reader (PerkinElmer EnVision 2105).55 Data were analyzed with GraphPad Prism 9.0 ...
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bioRxiv - Cell Biology 2024Quote: ... we used the following secondary antibodies: HRP-labeled goat anti-rabbit and mouse IgG (1:10000, NEF812 and NEF822, PerkinElmer, Waltham, MA). For immunofluorescence ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The microplates were incubated at room temperature for 1 hour and then the luminescent signal was detected on ViewLux plate reader (PerkinElmer, Waltham, MA).
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bioRxiv - Cancer Biology 2024Quote: ... The cells were then pulsed for 1 h with 200 μCi/ml of [35S]-Met(methionine)/[35S]-Cys(Cysteine) (Perkin Elmer, NEG072002MC) and chased for 0 ...
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Wnt-dependent activation of ERK mediates repression of chondrocyte fate during calvarial developmentbioRxiv - Developmental Biology 2021Quote: ... The slides were then washed in 1X TBS-T and incubated in HRP-conjugated goat-anti-rabbit secondary (1:1500, PerkinElmer NEF812001EA, Akoya Biosciences) for 45 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Luminescence was measured at 1 h post PathHunter© detection reagent addition using Victor3 plate reader (Perkin Elmer 0.5-s/well integration time). Data were normalized to control treated with the highest concentration of ligand (100%) ...
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bioRxiv - Neuroscience 2020Quote: ... Cux2 (Atlas Antibodies, Stockholm, Sweden; rabbit, 1:200; detected with ABC kit [Vector Laboratories, Burlingame, CA, USA] and TSA [PerkinElmer, Waltham, MA, USA]), Dcx (Santa Cruz Biotechnology ...
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bioRxiv - Bioengineering 2020Quote: ... cells were resuspended in 100 μL of medium at a 4:2:1:1:1 ratio and transferred to ultralow attachment (ULA) round bottom 96-well plates (CellCarrier Spheroid ULA 96-well Microplates, PerkinElmer, Waltham, MA, USA). In another method ...
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bioRxiv - Cell Biology 2021Quote: ... sections were washed in TBST and incubated with Alexa Fluor® 594 AffiniPure Goat Anti-Rabbit IgG (H+L) (Abacus DX, Australia) with Spectral DAPI (FP1490) added (1/1000) (Perkin Elmer, USA) for one hour at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... Organoid/hydrogel cultures were serum starved overnight before treatment with EGF (20 ng/mL) and promegestone/R5020 (1, 10, 100 nM; Perkin Elmer, Cat. #: NLP00400) and 10 nM R5020 with or without the addition of FAK (1 μM ...
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bioRxiv - Immunology 2020Quote: ... The IgGs and ACE2 were incubated in the plates for 1 hour and ACE2-Fc-Biotin was detected by DELFIA Eu-N1 Streptavidin (Perkin Elmer, 1244-360) using the DELFIA-TRF system (Perkin Elmer).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Oxaliplatin loaded Cedrus libani Sporopollenin (CD-SP-OX) were analyzed in the range of 4000-650 cm-1 using an FT-IR spectrometer (Perkin Elmer 100, USA) under ambient conditions.
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bioRxiv - Developmental Biology 2020Quote: ... we followed the protocol provided by the commercial company except that all samples were stained in 1 to 80 dilution of Cy3-conjugated or FLU-conjugated Tyrimide substrates for 60 minutes (Perkin Elmer NEL753001 Kit).
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bioRxiv - Cell Biology 2020Quote: In vitro kinase reactions were developed in a final volume of 50 μl containing 0.02 mM [γ-32P]ATP (1 µCi per tube, Perkin Elmer, Massachusetts, USA), 50 mM Tris-HCl ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were washed again in PBS with 0.1% Tween-20 six times for 1 hour and incubated in fluorescein tyramide reagent (Perkin Elmer, Cat. No. SAT701001EA) diluted 1:70 in amplification diluent for 50 minutes at room temperature ...
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bioRxiv - Genomics 2024Quote: ... size-selected sample was reamplified with 25 μL of NEXTFLEX PCR Master Mix 2.0 and 2 μL of 1:10 diluted NEXTFLEX Primer Mix 2.0 as described in the NEXTFLEX Cell free DNA-Seq Kit (V2) (Perkin Elmer, Pittsburgh, Pennsylvania, USA), step C PCR amplification ...
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bioRxiv - Microbiology 2024Quote: ... pseudoviruses were added in quadruplicate on HEK293T-ACE2 target cells which were seeded at a density of 1 × 104 cells/well in 96-well luminometer-compatible tissue culture plates (PerkinElmer, Waltham, MA, USA) 24h before infection ...
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bioRxiv - Developmental Biology 2022Quote: ... They were stored in PBS + 0.1% w/v sodium azide before imaging on a X-ray micro-CT device (Quantum FX Caliper, Life Sciences, Perkin Elmer, Waltham, MA) hosted by the PIV Platform ...
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bioRxiv - Bioengineering 2024Quote: ... MDA-MB-231 cells expressing HMGB1-GFP and CRT-RFP (stained MDA cells) were cultured in 100 µl of RPMI medium (10% FBS and 1% penicillin) in 96-well plate (PerkinElmer ViewPlate – 96 TC) at the density of 5 × 103 cells/well ...
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bioRxiv - Microbiology 2021Quote: ... in a ratio of 5:95 and analyzed at the mid-IR region (400-4,000 cm-1) of the spectrometer (Perkin Elmer Spectrum 100, United States). The biodegraded products of AB1 were identified using LC-MS analyses (Dionex Ultimate 3000 ...