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Citations for PerkinElmer :
951 - 1000 of 1075 citations for Primary Human Coronary Artery Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... The AlphaLISA® SureFire® Ultra™ assay was run in total volume 20 μL (using only 10 μL cell culture lysate sample) in 384 well plate (PerkinElmer, #6007290) following standard assay two step no-wash protocol ...
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bioRxiv - Cell Biology 2021Quote: Parental and SETD2-deleted U2OS cells were seeded into 384-well plates (750 cells in 75 μl per well) using the Janus Liquid Handling Workstation (Perkin Elmer) and incubated at 37°C overnight ...
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bioRxiv - Cell Biology 2020Quote: ... Radioactivity of both the supernatant and the cells was measured by the 2480 Wizard2 Automatic Gamma Counter (PerkinElmer, Waltham, MA, USA). Uptake values were adjusted for tracer decay and corrected for cell amount.
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bioRxiv - Cell Biology 2021Quote: ... and dead cells (stained by PI+) were imaged every hour for the indicated time with the Operetta CLS High-Content microscope (Perkin Elmer) at 40x Air/0.6 NA ...
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bioRxiv - Cell Biology 2021Quote: ... samples of doublets were allocated by semi-automated 3D intensity quantification in the Wnt3a-bead proximal and -distal cell using Volocity (Perkin Elmer) or Fiji (ImageJ) ...
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bioRxiv - Microbiology 2022Quote: ... Cells were imaged and percent of virus infected cells calculated using the Operetta High Content Imaging System and Harmony® High Content Imaging and Analysis Software (PerkinElmer).
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bioRxiv - Microbiology 2022Quote: ... Cytotoxicity was measured by adding resazurin [alamar blue (ThermoFisher)] diluted to 1x in DMEM according to the manufacturor’s protocol and incubated on cells for 2-4hr and reading fluorescence on a Victor 1420 Multilabel plate reader (Perkin Elmer) with excitation at 560 nM and emission at 590 nM.
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bioRxiv - Cancer Biology 2022Quote: MDA-MB-231 cells expressing mTurquoise2-SLBP(18-126) and H1-Maroon1 were seeded on clear flat-bottom 96-well plates (Perkin Elmer), treated with DMSO or PXS5382 40μM ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were incubated at restrictive temperature (37°C) for 3 h and finally transfer to 384-well CellCarrier plates (PerkinElmer, 6007550) for imaging on an Opera QEHS high-content screening microscope (PerkinElmer) ...
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bioRxiv - Neuroscience 2021Quote: ... Absorbance of 100 µl aliquots of solubilized cells was measured at 595 nm with Wallac Victor 1420 microplate reader (Perkin Elmer).
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bioRxiv - Neuroscience 2021Quote: ... the fluorescently labeled axon and dendrites were traced from the PV basket cell soma with a Nipkow spinning disk confocal microscope (Volocity, Perkin Elmer, equipped with an Orca camera ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The plate was sealed with a transparent film and cells were imaged with the Operetta High Content Imager (PerkinElmer Life Sciences) using a 20x objective ...
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bioRxiv - Cell Biology 2020Quote: Two days after transfection the Hek293 cells were washed with PBS and seeded into a 96 well black-white iso plate (Perkin Elmer) in pbs containing 5 mM glucose and supplemented with 5 µM of Nanoglo (Promega #N1120 ...
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bioRxiv - Molecular Biology 2019Quote: HUVEC cells were seeded in 6 cm2 plates (4 x 105 cells/well) and 8 hours after plating were pulsed for 2 hours with tritiated uridine (Uridine [5,6-3H]; Perkin Elmer; NET367001MC) at final concentration of 2 µCi/ml ...
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bioRxiv - Physiology 2019Quote: ... Cells were incubated with an anti-ERα antibody (F-10) overnight followed by Cy3 Tyramide signal amplification (PerkinElmer-TSA-Plus-Cy3) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... The lysed cells were transferred to vials and measured in a gamma counter (Wallac 1470 Wizard, Perkin Elmer, Waltham, MA, USA). Obtained cpm values were plotted with GraphPad Prism and the data fitted using nonlinear regression (one site - fit Ki ...
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bioRxiv - Microbiology 2020Quote: ... Cells were incubated for 16–18 hours at 37°C before Fluc activity in cell lysates was detected using a plate luminometer (EnSpire 2300 Multilabel reader by Perkin Elmer) and the Bright-Glo Luciferase Assays System (Promega) ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then washed with KREBS solution and fluorescence was read with the plate reader VICTOR® Multilabel Plate Reader (PerkinElmer). Data were normalized to the total amount of protein measured by the Bradford assay kit (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2021Quote: ... For neutralization assays 104 293T-hACE2 cells were plated in 100uL media per well in 96 well white-wall white-bottom plates (Perkin Elmer) and incubated overnight at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: Nascent proteins in U-2 OS cells expressing GFP-G3BP1 were labeled with 35S-met and -cys (EXPRE35S35S Protein Labeling Mix, Perkin Elmer) for 30 minutes in the presence of DMSO (0.1%) ...
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bioRxiv - Cancer Biology 2020Quote: ... Signals from luciferase-tagged cells were monitored at day 0 after injection and weekly by in vivo imaging using IVIS Lumina II (Perkin Elmer) for 5 weeks ...
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bioRxiv - Biophysics 2020Quote: ... The undifferentiated cells were seeded to 60% confluency to adhere for 24 h in a CellCarrier-96 Ultra Microplates (Perkin Elmer), which was followed by the media replacement with a fresh media containing FCS reduced to 1% and supplemented with 10 μM trans-Retinoic acid (Abcam ...
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bioRxiv - Cancer Biology 2021Quote: ... sections were stained for NICD1/KRT14/DNA or NICD1/Ki67/DNA and epithelial thickness measured and cell counting performed using Volocity 6 software (Perkin Elmer). Cell density was measured by counting the number of nuclei identified across the width of the epithelium on high resolution images of cryosections stained with DAPI and keratinocyte markers such as KRT4 and ITGA6 and dividing this number by the measured surface area.
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bioRxiv - Cell Biology 2021Quote: The data was collected in a set of 2 experiments for 9 compounds as follows: 384 well plates (PE cell carrier ultra, PerkinElmer, 6057308) containing 12,000 cells per well in RPMI1640 medium (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... Media supernatants of the 4T1 cells in the plates were collected and immediately analyzed with ATPlite one-step luminescence assay kits (PerkinElmer, MA) for the measurement of ATP concentration in the media ...
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bioRxiv - Immunology 2022Quote: ... incubated for 45 minutes at 37 °C and added to pre-seeded 293 flpin-TMPRSS2-ACE2 cells (made by Adrian Creanga, VRC, NIH) in triplicate in 96-well white/black Isoplates (Perkin Elmer). Following 2 hours of incubation ...
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bioRxiv - Synthetic Biology 2022Quote: ... cells were incubated with the AAVs for 24 hours at 37°C and assayed with Britelite plus (Perkin Elmer, cat#6066766) following the manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: Vero E6 cells were grown in DMEM supplemented with 10% FBS and seeded into clear bottom white 96 well plates (PerkinElmer, 6005688) at a density of 20,000 cells per well ...
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bioRxiv - Neuroscience 2022Quote: ... The medium was removed and cells were incubated with 500 nM of SNAP-Red in Tag-Lite Buffer (Perkin Elmer Cisbio) for 1 hr at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... 30,000 cells were seeded in each well of an ultra-low adhesive U-shaped 96-well plate (CellCarrier™ −96, PerkinElmer) with 200 ml of complete Neurobasal medium ...
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bioRxiv - Genomics 2022Quote: ... Total RNA was extracted from the female Epstein-Barr virus transformed B-lymphocyte cell line GM24143 using the Chemagic™ RNA TissuelO Kit H96 (PerkinElmer) following manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... the medium was removed from all wells and cells were lysed by the addition of 100 µl of a 1:1 dilution of BriteLite assay readout solution (Perkin Elmer) and 1xDPBS (Gibco ...
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bioRxiv - Biochemistry 2023Quote: ... half the medium was removed from all wells and cells were lysed by the addition of 50 µl of BriteLite assay readout solution (Perkin Elmer) to make a a 1:1 dilution into each well ...
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bioRxiv - Biochemistry 2023Quote: ... Binding reactions were incubated for 2 hours and then terminated by washing the solutions over a PEI-coated GF/C filter using a cell harvester (Perkin Elmer) followed by wash steps using ice-cold buffer [50 mM HEPES ...
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bioRxiv - Cancer Biology 2023Quote: Cells were seeded at 200 cells per well in 40 μL in the inner-312 wells of 384-well white-walled culture plates (Perkin Elmer) and allowed to attach for 24-hours prior to drug treatment ...
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bioRxiv - Cancer Biology 2023Quote: For 2D adherent studies cells were seeded at 500-1,000 cells per well in 100 μL in the inner-60 wells of 96-well white-walled culture plates (Perkin Elmer) and allowed to attach for 48 hours prior to drug treatment ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were seeded at 500 cells per well in 100 μL in the inner-60 wells of 96-well white-walled culture plates (Perkin Elmer) and allowed to attach for 24 hours prior to drug treatment ...
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bioRxiv - Cancer Biology 2023Quote: ... We verified that these cells did not lose viability after overnight culture in serum-free medium relative to medium with 10% serum as determined by cell-based measurements of CBG bioluminescence in an IVIS Lumina (Perkin Elmer). We injected 1 × 105 breast cancer cells per mouse (n = 5 per each cell type) ...
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bioRxiv - Cancer Biology 2023Quote: ... and then acquired a bioluminescence image of total viable cells on an IVIS Lumina (30 second image, large field of view) (Perkin Elmer). A person blinded to experimental conditions enumerated colonies and quantified imaging data (Living Image ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed twice and cell-associated activity was subsequently measured in a well-type gamma-counter (Wallac 2480 wizard, Perkin Elmer). In a separate binding assay equimolar concentrations (1.97 pM ...
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bioRxiv - Genomics 2023Quote: ... Plates were shaken and incubated for 3h at cell culture conditions before measuring the fluorescence (560Ex/590Em) on an EnVision Multilabel Plate Reader (Perkin Elmer). The lowest fluorescence from the PAO killing control in each replicate was subtracted from all the samples within the sample replicate to standardize the raw values ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The lysed cells were transferred to vials and measured in a gamma counter (Wallac 1470 Wizard, Perkin Elmer, Waltham, MA, USA). Obtained cpm values were plotted with GraphPad Prism 7 and the data were fitted using nonlinear regression (one site - fit Ki ...
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bioRxiv - Pathology 2023Quote: ... as previously described [71] using Multiplex Fluorescent Reagent Kit V2 (Advanced cell Diagnostics) and TSA Plus Cyanine 3 and Cyanine 5 (Perkin Elmer) as fluorophores according to manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2023Quote: MIN6 cells were seeded at a density of 15,000 per well in a 384-well plate (Perkin Elmer, CellCarrier Ultra, #6057300). After 24 h recovery ...
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bioRxiv - Immunology 2023Quote: ... cells were isolated ex-vivo and then incubated for six hours with 0.5 μCi 14C-palmitic acid (Perkin Elmer Cat# NEC075H050UC) in the following media ...
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bioRxiv - Cancer Biology 2023Quote: ... and 25μL was added to the transfected cells, after which the baseline luminescence was measured for 15min (continuous mode, 1min intervals) using an Envision (Perkin Elmer) spectrophotometer ...
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bioRxiv - Immunology 2023Quote: Vero E6 cells were grown in DMEM supplemented with 10% FBS and seeded into white-walled 96 well plates (PerkinElmer, 6005688) at a density of 20,000 cells per well ...
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bioRxiv - Cell Biology 2024Quote: ... Cholesterol efflux was determined as % counts per minute (CPM) in the supernatant versus cell lysates with a liquid scintillation analyzer (Tricarb-2810TR, Perkin Elmer).
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bioRxiv - Molecular Biology 2023Quote: ... were cultured at 6000 cells per well in DMEM (+10% FBS, +1% P/S) on Perkin Elmer LLC CellCarrier-96 Ultra Microplates (Perkin Elmer) coated with PLO overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... The luciferase reporter expression in cancer cells was examined based on bioluminescence using the IVIS imaging system (PerkinElmer, Hopkin, MA, USA).