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Citations for PerkinElmer :
951 - 1000 of 1088 citations for 4 2 Hydroxyimino 2 4 methoxy phenyl ethyl piperazine 1 carboxylic acid tert butyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Plates were incubated at RT for 1 h and the fluorescence intensity measured at 620 nm and 665 nm by an EnVision multilabel plate reader (PerkinElmer).
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bioRxiv - Biochemistry 2022Quote: ... Steady-state binding signal was obtained after incubation of reactions for 1 hr at 25° C using a EnSight Multimode plate reader (PerkinElmer) with a 30 ms excitation at 680 nm and emission measured at 615 nm after 140 ms ...
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bioRxiv - Immunology 2022Quote: ... and primary human GM-CSF monocyte derived macrophages (MDM) were cultured in collagen-1-coated CellCarrier Ultra 96-well black walled plates (PerkinElmer) at a ratio of 1425 HSC:28500 MDM in FBM (Lonza ...
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bioRxiv - Microbiology 2022Quote: ... 48 hpi liver-stage cultures were washed with PBS and lysed in Nano-Glo Luciferase Assay buffer containing Nano-Glo Luciferase Assay substrate diluted 1:200 and luminescence was measured after 3 minutes on an IVIS Specturum CT (PerkinElmer). To monitor merosome detachment at 65 hpi ...
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bioRxiv - Microbiology 2022Quote: The lipid compositions of the bacterial strains were determined after labeling with [1-14C] acetate (specific activity: 55 mCi/mmol; PerkinElmer) or [35S] sulfate (specific activity ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were washed three times in 1 x PBS and imaged using the Opera Phenix high-content microscope (Perkin Elmer) at 63x magnification.
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bioRxiv - Plant Biology 2023Quote: ... Radioactivity was measured from the extract and the trichloroacetic acid precipitated protein pellet for 5 min with a scintillation counter (Wallac 1414 WinSpectral v.1.40; Wallac Oy, Turku, Finland) using 1 ml of Ultima Gold™ (PerkinElmer) liquid scintillation cocktail ...
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bioRxiv - Biochemistry 2022Quote: ... 300 mM potassium acetate and 1 mM DTT followed by addition of 10 μM acetyl-CoA in a 1:10 ratio of 3H radiolabeled acetyl-CoA (PerkinElmer) to cold acetyl-CoA ...
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bioRxiv - Biochemistry 2023Quote: ... Reactions were initiated by adding an equal volume of assay buffer containing 1 μM [35S]GTPγS (~50 cpm/fmol, Perkin Elmer) at 30 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Hybridization signals were detected by a chromogenic reaction using red chromogen dilution 1:3000 (PerkinElmer TSA Plus Cyanine 3 System). RNA-staining signal was identified as red punctate dots and clusters ...
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bioRxiv - Plant Biology 2023Quote: ... RNA quantification was performed through spectrophotometric analysis at 260 nm using the Nanodrop ND-1000 Spectrophotometer and RNA quality was assessed using 1% agarose gel electrophoresis or the LabChip GX Touch (PerkinElmer). 5 µg of purified total RNA was combined with 5 µL of TURBO DNase buffer and 1 µL TURBO DNase (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... using a HyBEZ® oven Probes were detected using Tyramide signal amplification (TSA) reaction (1:600 dilution) using an Alexa-flour labeled TSA kit (PerkinElmer) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... cells were changed into 1 mL of starvation media containing 200 μCi/mL EasyTag EXPRESS35S Protein Labeling Mix (PerkinElmer NEG772002MC), grown for indicated time points ...
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bioRxiv - Immunology 2024Quote: ... the reaction was stopped with an equal volume of 1 M H2S04 and absorbance at 450 nm was quantified on a Victor X4 plate reader (PerkinElmer). Values reported are A450 means ± standard deviations across all dilutions falling within a valid calibration curve range that are at least two-fold above background and no more than 2.8.
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bioRxiv - Neuroscience 2024Quote: ... the membranes were incubated for 90 seconds in 1 mL of enhanced chemiluminescence (ECL Pro) reagent (catalog number NEL122001EA, PerkinElmer Health Sciences Canada Inc. ...
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bioRxiv - Biochemistry 2024Quote: ... supplemented with 10% FBS and 1% Sodium pyruvate and seeded at a density of 30,000 cells per well in Isoplate-96 plates (Perkin Elmer). Twenty-four hours after transfection with the wild-type or mutant hURAT1 ...
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bioRxiv - Biophysics 2021Quote: ... Depletion of NADPH is observed in 1 mL cuvettes with a path length of 1cm in a Lambda 650 UV/VIS spectrometer (Perkin Elmer) with attached water Peltier system set to 17°C ...
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bioRxiv - Cell Biology 2020Quote: ... Embryos were then washed in Maleic buffer and then PBS at room temperature prior to incubation with 50 µl amplification diluent with 1 µl Tyramide-Cy3 (Perkin Elmer) for 3 h at room temperature in the dark ...
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bioRxiv - Cell Biology 2020Quote: ... the wells were pulsed with 1 μCi/well of [methyl-3H]TdR (70–90 Ci/mmol; NEN Radiochemicals, PerkinElmer, Akron, OH). After 16 h ...
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bioRxiv - Developmental Biology 2021Quote: ... Heat mediated antigen retrieval was used to process sections (Vector, H-3300) and then samples were blocked for 1 hour in blocking buffer at room temperature (PerkinElmer, FP1020). Primary antibodies diluted in blocking buffer were incubated with samples overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... or 3.3 mM (Figure 5-figure supplement 1 and 8) ATP and 5 µM ProfilerPro Kinase Peptide Substrate 11 5-FAM-KKLNRTLSVA-COOH (PerkinElmer, U.S.A.), in the presence or absence of 0.66 µM CaM (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... LPS-stimulated plasmablasts were starved for methionine and cysteine for 60 minutes and pulse labeled for 15 minutes with 55 µCi/1×106 cells of Express 35S protein labeling mix (Perkin Elmer). Samples were then diluted 10x with cell culture media containing 5mM unlabeled methionine and cysteine ...
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bioRxiv - Genomics 2020Quote: ... 63%, 40%, 25%) with 9:1 CSBL1:CBL1 buffer (Zeptosens, Bayer) using a Sciclone Caplier ALH3000 liquid handling root (Perkin Elmer). Diluted samples were spotted onto ZeptoChips (Zeptosens ...
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bioRxiv - Genetics 2021Quote: Cells were fixed in growth medium with 3.7% formaldehyde at room temperature for 30 minutes and their cell walls digested with a solution containing 50 μg/ml zymolyase and 1:50 glusulase (Perkin Elmer). The digested cells were mounted on poly-L lysine coated slides to immobilize them for microscopy ...
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bioRxiv - Genomics 2020Quote: ... 5 mM MgCl2, 1 mM DTT, 300 mM KCl, 40 µM ATP, 40 µM GTP, 40 µM Biotin-11-CTP [Perkin Elmer] ...
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bioRxiv - Immunology 2021Quote: TZM-bl target cells (NIH AIDS reagent program) were seeded at a density of 1×104 cells/well in 96-well luminometer-compatible tissue culture plates (Perkin Elmer) 24h before infection ...
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bioRxiv - Microbiology 2021Quote: ... 1 mL of cells from each culture pulsed with 1 μCi [γ32P]-ATP having a specific activity of 30 Ci/mmol (Perkin Elmer) for 5 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... 293T-ACE2 target cells were seeded at a density of 1×104 cells/well in 96-well luminometer-compatible tissue culture plates (Perkin Elmer) 24h before infection ...
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bioRxiv - Biochemistry 2020Quote: ... Fifty μl of each elution fraction was suspended in 1 ml of liquid scintillation reagent (Ultima Gold, PerkinElmer, Wellesley, MA, USA), and the radioactivity in each vial was measured using a scintillation counter ...
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bioRxiv - Microbiology 2021Quote: ... 293T-ACE2 target cells were seeded at a density of 1×104 cells/well in 96-well luminometer-compatible tissue culture plates (Perkin Elmer) 24h before infection ...
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bioRxiv - Biochemistry 2021Quote: ... and mRNA was translated for 1 hour at 30 °C in presence of 10 µCi/µL EasytagTM 35S Express Protein Labeling Mix (Perkin Elmer). The mixtures were centrifuged for 2 min at 10,000 x g at 4 °C after inhibiting protein synthesis with 1 mM cycloheximide and translocated CFTR was retrieved from the pellet fraction ...
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bioRxiv - Plant Biology 2020Quote: ... the remaining root parts were collected and ground completely in liquid nitrogen and homogenized in 1 mL scintillation solution (PerkinElmer, 6013199). The samples were incubated overnight to allow the radioactivity to evenly diffuse into the whole volume of the scintillation cocktail ...
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bioRxiv - Microbiology 2020Quote: ... 293T-ACE2 target cells were seeded at a density of 1× 104 cells/well in 96-well luminometer-compatible tissue culture plates (Perkin Elmer) 24 h before infection ...
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bioRxiv - Microbiology 2020Quote: ... target cells were seeded at a density of 1×104 cells/well in 96-well luminometer-compatible tissue culture plates (Perkin Elmer) 24 hours before infection.
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bioRxiv - Neuroscience 2020Quote: ... was used as a secondary fluorophore for C1 probes (Gtpbp2, Slc7a1, Chac1) and TSA® Plus Cyanine 3 (PerkinElmer, 1:2,000) was used as a secondary fluorophore for C2 probes (Gtpbp1 ...
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bioRxiv - Microbiology 2020Quote: ... CoV-infected cells and control mock-infected cells were incubated for different periods of time with tritiated uridine ([5-3H]uridine, 1 mCi/ml, Perkin Elmer), which was mixed in a 1:1 ratio with double-concentrated medium ...
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bioRxiv - Molecular Biology 2022Quote: ... 1mM PMSF, 100mM KCl and 1 mCi of [3H]AdoMet (S-adenosyl-L-[methyl-3H] methionine, 80 Ci/mmol; SAM) (Perkin Elmer) or 0.15mM unlabeled S-adenosyl methionine for 45 min at 30°C ...
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bioRxiv - Microbiology 2020Quote: ... 293T-ACE2 target cells were seeded at a density of 1×104 cells/well in 96-well luminometer-compatible tissue culture plates (Perkin Elmer) 24h before infection ...
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bioRxiv - Microbiology 2020Quote: ... [methyl-14C] CDP-choline (50–60 mCi/mmol) and [diarachidonyl-1-14C] PC (50–60 mCi/mmol) were purchased from Perkin Elmer, Inc (Waltham ...
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bioRxiv - Immunology 2021Quote: ... 293T-ACE2 target cells were seeded at a density of 1×104 cells/well in 96-well luminometer-compatible tissue culture plates (Perkin Elmer) 24h before infection ...
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Peripheral neuropathy linked mRNA export factor GANP reshapes gene regulation in human motor neuronsbioRxiv - Neuroscience 2021Quote: ... were hybridized for 2 h at 40°C followed by signal amplification and developing the HRP channel with TSA Plus fluorophore Cyanine 3 (1:1500) (NEL744001KT, Perkin Elmer) according to the manual ...
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bioRxiv - Neuroscience 2021Quote: ... The signal of CCK hybridization was revealed by a TSA reaction using fluorescein isothiocyanate (FITC)-labeled tyramide (1:100 for 10 minutes, NEL741001KT Perkin Elmer). After several washes ...
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bioRxiv - Cancer Biology 2020Quote: ... membranes were washed and probed with horseradish peroxidase (HRP)-conjugated secondary antibodies for 1 hour before detection with Western Lightning Chemiluminescence (Perkin Elmer). Antibodies used included ...
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bioRxiv - Microbiology 2021Quote: ... 293T-ACE2 target cells were seeded at a density of 1×104 cells/well in 96-well luminometer-compatible tissue culture plates (Perkin Elmer) 24h before infection ...
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bioRxiv - Synthetic Biology 2020Quote: ... Each reaction was diluted in TE and mixed with a PicoGreen working stock (diluted to 1/200× in TE Buffer) to be read with an Enspire plate reader (Perkin Elmer) with 480 nm excitation and 520 nm emission filter ...
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bioRxiv - Immunology 2021Quote: ... target cells were seeded at a density of 1 × 104 cells/well in 96-well luminometer-compatible tissue culture plates (Perkin Elmer) 24 h before infection ...
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bioRxiv - Microbiology 2020Quote: ... 293T-ACE2 target cells were seeded at a density of 1×104 cells/well in 96-well luminometer-compatible tissue culture plates (Perkin Elmer) 24h before infection ...
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bioRxiv - Cell Biology 2020Quote: ... subconfluent HDLECs cultured in 12-well plates were incubated with 1 ml per well EBM2 medium (containing appropriate amounts of serum and supplement) with [5-3H]-glucose (Perkin Elmer) for 2-3 hours ...
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bioRxiv - Genomics 2021Quote: ... the anti-DIG-HRP antibody was detected with the TSA-Cy3 substrate (1:50, TSATM PLUS Cy3 kit, NEL 745001KT, Perkin Elmer) for 10 min at room temperature ...
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bioRxiv - Physiology 2020Quote: ... glycosylphosphatidylinositol-anchored high-density lipoprotein binding protein 1 (GPIHBP1) knock out mice were orally gavaged with 100 μCi of [9,10-3H(N)]-Triolein (Perkin Elmer, NET431001MC) suspended in olive oil ...