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Citations for PerkinElmer :
51 - 100 of 109 citations for Palmitic acid U 13C16 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... cultures were grown with 2.5 μCi/mL of 32P orthophosphoric acid (32P) (Perkin Elmer) and lipid A was extracted ...
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bioRxiv - Developmental Biology 2022Quote: ... The composition of fatty acids was analyzed by gas chromatography (Perkin Elmer Clarus 680) employing a flame ionization detector with an SP2300 fused silica capillary column (30 m × 0.25 mm × 0.2 μm ...
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bioRxiv - Bioengineering 2022Quote: ... Volatile fatty acid (VFA) analysis was done in gas chromatography (PerkinElmer LLC, MA, USA) equipped with flame ionization detector (FID ...
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bioRxiv - Genetics 2024Quote: ... Total nucleic acid was extracted from the tissues using the Chemagic 360 instrument (PerkinElmer) and quantified with Qubit DNA High Sensitivity Kit (Catalogue Number ...
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bioRxiv - Neuroscience 2024Quote: ... Profiling libraries were assessed with LabChip GX Touch HT Nucleic Acid Analyzer (PerkinElmer, Revvity), with HT DNA NGS 3K Reagent Kit (PerkinElmer ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: The analysis of the selected fractions was performed using Clarus 680 (Perkin Elmer. U. S. A) GC-MS because GC interfaced to a mass spectrometer (GC-MS ...
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bioRxiv - Biochemistry 2023Quote: ... in the presence of 20 µM of the corresponding L-[14C]-amino acid (Perkin Elmer). AaRSs were diluted in 100 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Immunology 2024Quote: ... samples were boiled for 30 min in 10 mM citric acid (AR6, PerkinElmer, Waltham, MA) to expose epitopes ...
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bioRxiv - Microbiology 2020Quote: ... 100 μCi ml−1 [32P] phosphoric acid added to label newly synthesized RNA (Perkin Elmer #NEX053H). RNA was harvested in Trizol (Thermofisher #15596018 ...
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bioRxiv - Physiology 2020Quote: ... to be read using a LabChip GX Touch Nucleic Acid Analyser (Perkin Elmer, Cat. No. CLS138162). Reverse transcription and amplification of RNA purified from isolated cells was performed with the NuGEN Ovation RNA-Seq System V2 (NuGEN Cat ...
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bioRxiv - Genetics 2024Quote: ... PCR products underwent fragment size verification using LabChip® GX Touch™ Nucleic Acid Analyzer (PerkinElmer) and 1% agarose gel electrophoresis ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5 mM DTT, 0.5 % Sarkosyl, 0.4 u/μl RNase inhibitor, 0.025 mM biotin-ATP/CTP/GTP/UTP [Perkin Elmer]). Equal amounts of nuclei extracted from Drosophila S2 cells were used as spike-in material in run-on reactions ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.5 mM DTT, 0.5% Sarkosyl, 0.4 U/µl RNase inhibitor, 0.025 mM biotin-ATP/CTP/GTP/UTP [Perkin Elmer]). Total RNA was extracted using Trizol and ethanol precipitation ...
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DIFFERENTIAL BIOENERGETICS IN ADULT RAT CARDIOMYOCYTES ISOLATED FROM THE RIGHT VERSUS LEFT VENTRICLEbioRxiv - Cell Biology 2020Quote: Fatty acid oxidation was measured using freshly isolated cardiomyocytes incubated in suspension with 14C-palmitate (Perkin Elmer) conjugated to BSA ...
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bioRxiv - Microbiology 2021Quote: ... and Tris buffer containing trace amount of 32P-labeled orthophosphoric acid (∼1.2 pmol and ∼1 µCi, PerkinElmer) and different concentrations of cold phosphate (NaH2PO4 ...
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bioRxiv - Microbiology 2023Quote: ... An aliquot of the supernatant was processed for total nucleic-acid extraction using Chemagic 360 (Perkin Elmer). Nucleic acid preparations from wastewater samples are known to contain PCR inhibitors that interfere with their accurate quantification using PCR-based methods ...
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bioRxiv - Immunology 2022Quote: ... the reaction was stopped with 1M phosphoric acid and OD450 was measured by multimode microplate reader (PerkinElmer EnVision 2105 ...
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bioRxiv - Immunology 2023Quote: ... cells were labeled with 0.5 uCi of 56 mCi/mmol [14C] acetic acid (Perkin Elmer Cat# NEC084H001MC) per well overnight ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: The GC-MS analysis of the effective extracts was performed using Clarus 680 GC (Perkin Elmer. U. S. A) and the GC interfaced to a mass spectrometer (GC-MS ...
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bioRxiv - Cell Biology 2022Quote: Candidate small RNA sequences were identified from raw sequence data using cutadapt (ver. 2.8)21 with parameters “-u -4 -a A(10)” as recommended by PerkinElmer NextFlex Combo-Seq kit ...
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bioRxiv - Plant Biology 2022Quote: ... dissolved in 0.1% nitric acid and 4 mL Optima Gold XR scintillant (Perkin Elmer, Glen Waverley, VIC, Australia) was added prior to counting for two minutes using a Liquid Scintillation Counter (LS6500 ...
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bioRxiv - Cancer Biology 2023Quote: ... Total nucleic acid was extracted from FFPE tissue sections using Chemagic 360 sample-specific extraction kits (Perkin Elmer). Percent tumor cellularity as a ratio of tumor to normal nuclei was verified against pathologist-derived assessment ...
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bioRxiv - Neuroscience 2020Quote: ... single organoids were transferred to ultra-low attachment 96-well U-bottom wells (CellCarrier-96 Spheroid ULA, Perkin Elmer 6055330) in carryover medium ...
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bioRxiv - Microbiology 2024Quote: ... Of each sample three replicates and two formaldehyde-fixed (final conc. 1.85 %) adsorption blanks were spiked with [methyl-3H]-thymidine and L-[14C(U)]-leucine (Perkin Elmer) at saturating concentrations of 20 nM and 150 nM ...
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bioRxiv - Cell Biology 2020Quote: Fully differentiated C2C12 MS2-CAS myotubes were starved in serum-and glucose-free DMEM for 2 hrs before a 3-hr incubation in low-glucose DMEM/fatty acid-free BSA 0.2% supplemented with radiolabeled glucose (Glucose, D-[3-3H]; 1 μCi/ml; PerkinElmer). Then ...
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bioRxiv - Microbiology 2021Quote: ... The reaction was then stopped with the addition of 0.16 N sulfuric acid and the absorbance measured at 450 nM on an EnSight multimode plate reader (PerkinElmer). Data were in-column normalized using cells containing serum only or no serum with buffer addition were used as 100% and 0% signal ...
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bioRxiv - Biochemistry 2021Quote: ... bound to albumin (1:1 molar ratio) or 0.1 μM [1-14C]oleic acid (2 μCi/dish Perkin Elmer) bound to albumin (1:1 molar ratio ...
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bioRxiv - Biochemistry 2021Quote: ... The radioactivity of the acid-insoluble pellet was measured through the addition of 50μL of MicroScint PS (Perkin Elmer), vortexing ...
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bioRxiv - Cell Biology 2021Quote: ... [9,10(n)-3H]oleic acid (OA) (54.6 Ci/mmol) and [14C]glycerol (250 μCi/mmol) were from Perkin Elmer. Inhibitors of DGAT1 (PF-04620110) ...
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bioRxiv - Biochemistry 2023Quote: ... 2-[1,2-3H(N)]-deoxy-D-glucose and [1,2-14C]-acetic acid were purchased from PerkinElmer (Waltham, MA, USA). PPARγ ...
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bioRxiv - Bioengineering 2023Quote: ... with primers from IDT (Leuven, Belgium) and analyzed on a LabChip® GX Touch Nucleic Acid Analyzer (Perkin Elmer). Plasmids with correct fragment size were amplified by PCR using Q5® High-Fidelity DNA polymerase (NEB ...
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bioRxiv - Microbiology 2024Quote: Overnight cultures were diluted to an OD600 of ∼0.05 in 10 mL LB Miller broth supplemented with 5 µCi/mL 32P ortho-phosphoric acid (PerkinElmer) and grown until reaching an OD600 of ∼0.6 ...
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bioRxiv - Microbiology 2020Quote: ... log phase promastigotes were washed twice with PBS and resuspended at 5.0 x 107 cells/ml in PBS in the presence of 100 µM of D-[U-14C] glucose (250 mCi/mmol, PerkinElmer Inc.). The reaction mixture (200 µl each in triplicates ...
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bioRxiv - Molecular Biology 2020Quote: Nascent proteins in U-2 OS cells expressing GFP-G3BP1 were labeled with 35S-met and -cys (EXPRE35S35S Protein Labeling Mix, Perkin Elmer) for 30 minutes in the presence of DMSO (0.1%) ...
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bioRxiv - Cell Biology 2020Quote: ... subconfluent HDLECs cultured in 6-well plates were incubated with 2 ml per well EBM2 medium (containing appropriate amounts of serum and supplement) with [14C(U)]-glutamine (Perkin Elmer) for 6 hours ...
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bioRxiv - Microbiology 2024Quote: ... and the uptake of 14C(U) l-arginine was measured in the bacterial lysates using a liquid scintillation counter (Perkin Elmer).
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bioRxiv - Neuroscience 2023Quote: ... 30,000 cells were seeded in each well of an ultra-low adhesive U-shaped 96-well plate (CellCarrier™ −96, PerkinElmer) with 200 ml of complete Neurobasal medium ...
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bioRxiv - Microbiology 2024Quote: ... gingivalis wild-type strain was incubated in 5 mL of TSB or TSBNH (a nutritionally limited TSB media that lacks hemin) media with or without radiolabeled L-[14C(U)] Glutamate (1.0 mCi/mmol, Perkin Elmer # NEC290E050UC) at 37°C under anaerobic condition ...
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bioRxiv - Cell Biology 2024Quote: Hep G2 and U-2 OS cells are counted and seeded into 384 well plates (PhenoPlate 384-well, Perkin Elmer, 6057328) using a Biotek or Multidrop microplate dispenser ...
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bioRxiv - Biochemistry 2021Quote: Structural models of polyenoyl tetramic acids 3 and 4 were built and energy minimized using Chem3D v.16.0 (Perkin Elmer). The terminal region of polyenes ...
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bioRxiv - Physiology 2020Quote: ... The integrity of resultant cDNA was assessed using a LabChip GX Touch 24 Nucleic Acid Analyzer as per manufacturer’s instructions (Perkin Elmer).
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bioRxiv - Immunology 2022Quote: ... Quality and quantity were checked using a Caliper LabChip GX Touch HT Nucleic Acid Analyzer (PerkinElmer, Waltham, MA 02452 USA). The PCR product library was quantitated and subjected to sequencing on an Illumina MiSeq sequencer (Illumina ...
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bioRxiv - Immunology 2022Quote: ... using the RNA Assay and DNA 5K/RNA/CZE HT Chip on a LabChip GX Touch HT Nucleic Acid Analyzer (PerkinElmer). TruSeq Stranded mRNA Library Prep kit (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... DNA quantification and quality check was done using a LabChip GX Touch HT Nucleic Acid Analyzer (PerkinElmer, Waltham, MA, USA). Libraries were prepared as detailed on the Illumina® support website (http://support.illumina.com ...
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bioRxiv - Biochemistry 2023Quote: ... Protein biosynthesis rates were determined by measuring the incorporation of radioactive methionine in auranofin-treated and untreated samples after protein precipitation with 20 % (w/v) trichloro acetic acid using a scintillation counter (Tri-Carb 2800TR, PerkinElmer). 55 µg of protein from pulse-labeled samples for analytical gels and 300 µg of protein from unlabeled samples for preparative gels were rehydrated into IPG strips (24 cm ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 3.7 MBq/mL) and [2-14C] acetic acid (2.183 GBq/mmol, 37 MBq/mL) were purchased from PerkinElmer (Waltham, MA). For the ultra[performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS ...
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bioRxiv - Microbiology 2024Quote: ... Quality and quantity were checked using a Caliper LabChip GX Touch HT Nucleic Acid Analyzer (PerkinElmer, Waltham, MA 02452 USA). The PCR product library was quantitated and subjected to sequencing on an Illumina MiSeq sequencer (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... Quality and quantity were checked using a Caliper LabChip GX Touch HT Nucleic Acid Analyzer (PerkinElmer, Waltham, MA 02452 USA). The PCR product library was quantitated and subjected to sequencing on an Illumina MiSeq sequencer (Illumina ...
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bioRxiv - Cancer Biology 2020Quote: ... proteins were first labeled for 48 h with 0.25 μM Ci ml-1 L-[U-14C] VALINE (Perkin Elmer, Waltham, MA, USA) in GIBCO-RPMI 1640 medium (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... Embryoid body differentiation was initiated on day 0 by detaching 70-80% confluent iPSCs with 0.5 mM EDTA and seeding 9 000 cells/well on ultra-low attachment U-bottom 96-well plates (CellCarrier-96 Spheroid ULA, Perkin Elmer 6055330) in 150μl of E8+0.5% P/S supplemented with 20 μM ROCK inhibitor ...