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Citations for PerkinElmer :
51 - 100 of 110 citations for NSG2 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... and blocked by TN Blocking buffer (TSA Plus Fluorescence System; PerkinElmer, MA, USA). Primary antibodies were incubated for more than 18 hr at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.1% Tween20) and then blocked in TNT/block [0.5% blocking reagent (PerkinElmer FP1012)/TNT] for 1 hour at RT before ON incubation with anti-DIG (1:100 ...
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bioRxiv - Immunology 2022Quote: ... Unspecific antibody binding on sorted cells was blocked with TSA blocking reagent (PerkinElmer) for 30 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by PBS washing and treated with TNB blocking reagent (Perkin Elmer, FP1012) for 1 hour at RT ...
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bioRxiv - Neuroscience 2024Quote: ... incubated in TNB (Blocking Reagent [Perkin Elmer; 0.05% in TN]; 30 min, RT), incubated with anti-DIG-POD antibody (Roche ...
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bioRxiv - Immunology 2021Quote: ... Peptide masses were initially predicted by ChemDraw Professional 15.0 (PerkinElmer, Massachusetts, USA) and compared to acquired masses after LC-MS measurements.
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bioRxiv - Microbiology 2021Quote: ... an equal volume of biotinylated SRC1 peptide/Europium-labeled streptavidin (Perkin Elmer) was added to biotynlated RORγ/APC-labeled strepavidin (Perkin Elmer) ...
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bioRxiv - Microbiology 2021Quote: ... pre-incubated with blocking buffer (TNB) supplied in the TSA Plus kit (Perkin Elmer) for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... Tissues were blocked in 3% hydrogen peroxide followed by TSA blocking buffer (Perkin Elmer #FP1012). Tissues were then incubated with rabbit Sera against MmuPV1 L1 (provided by Dr ...
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bioRxiv - Biochemistry 2020Quote: ... Peptide stocks in DMSO were spotted onto 384-well white OptiPlates (PerkinElmer Life Sciences) at concentration ranges of 1 nM to 100 M using a Labcyte Echo 555 Omics acoustic liquid handler (Labcyte ...
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bioRxiv - Bioengineering 2019Quote: ... Aliquots of the peptide were deposited on a “Golden Gate” diamond ATR (PerkinElmer, USA). PBS was used as a background spectrum ...
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bioRxiv - Neuroscience 2021Quote: ... slides were blocked with TNB solution (0.1 M Tris-HCl, pH 7.5, 0.15 M NaCl, 0.5% blocking reagent from PerkinElmer) for 1 h ...
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bioRxiv - Neuroscience 2020Quote: ... and then blocked with TNB solution (0.1 M Tris-HCl, pH 7.5, 0.15 M NaCl, 0.5% blocking reagent from PerkinElmer) for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... blocked with TNB blocking buffer (from TSA Plus Cyanine 3 System, NEL744001KT, PerkinElmer, Waltham, MA, US) for 1 hr at RT and incubated with anti-DIG-POD (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... rinsed in PBS-T for 30 minutes and then blocked with 0.5% blocking buffer (PerkinElmer, FP120) for 2 hours ...
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bioRxiv - Neuroscience 2021Quote: ... placed in 0.5 % H2O2 in PBS for 30 minutes and then blocked with TNB solution (0.1M Tris-HCl, pH 7.5, 0.15M NaCl, 0.5% blocking reagent from PerkinElmer, NEL701A001KT) for 1 hour ...
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bioRxiv - Biophysics 2022Quote: ... Single-tethered DNA was stretched with blocking buffer containing 1 µg/mL biotinylated anti-digoxigenin (Perkin Elmer) at 100 µL/min flow rate ...
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bioRxiv - Neuroscience 2020Quote: ... 3% TritonX-100) and TNB (0.1 M Tris-HCl, 0.15 M NaCl, 0.5% Perkin Elmer blocking reagent) buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Sections were blocked by incubation with Tris-NaCl blocking buffer (TNB) (0.1 M Tris-HCl, pH 7.5, 150 mM NaCl, and 0.5% w/v blocking reagent, PerkinElmer) for analysis of Podoplanin ...
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bioRxiv - Developmental Biology 2022Quote: ... and blocked for 1 hour in TNB buffer (0.1M Tris-HCl, pH 7.5, 0.15M NaCl, 0.5% Blocking reagent [PerkinElmer FP1020]) at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Compound plates containing synthetic peptides in DMEM/BSA were placed in a MicroBeta LumiJet luminometer (PerkinElmer). After loading ...
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bioRxiv - Molecular Biology 2021Quote: Peptide or small molecules stimulated cAMP accumulation was measured by a LANCE Ultra cAMP kit (PerkinElmer). Briefly ...
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bioRxiv - Neuroscience 2020Quote: ... Reactions were performed with 50 nM ULight-Glycogen Synthase (Ser641/pSer657) peptide (Perkin Elmer #TRF0131-M), 15 μM ATP ...
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bioRxiv - Molecular Biology 2022Quote: Peptide-or small molecule-stimulated cAMP accumulation was measured by a LANCE Ultra cAMP kit (PerkinElmer). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... The amount of peptide remaining bound was measured using scintillation counting in Ultima Gold (Perkin Elmer). Experiments were performed a minimum of three times to generate an overall mean and standard deviation ...
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bioRxiv - Biochemistry 2022Quote: ... The structures of NTV and the substrate peptide were drawn in ChemDraw 20.0 (Perkin Elmer, USA) and optimized to generate 3D conformations using the LigPrep module in Maestro.
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bioRxiv - Cancer Biology 2021Quote: ... The sections were then incubated in a Tris–NaCl-blocking buffer (TNB-buffer, FP1020, PerkinElmer, Waltham, MA, USA) for 30 min at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... For immunofluorescence staining sections were blocked with TNB blocking buffer (0.1 M Tris-HCl, pH 7.5; 0.15 M NaCl; 0.5% (w/v) blocking reagent (PerkinElmer, Cat # FP1020) for 30 min at RT ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were blocked with TNB solution (0.1 M Tris-HCl, pH 7.5, 0.15 M NaCl, 0.5% PerkinElmer TSA blocking reagent) 30 min and incubated in primary antibodies overnight ...
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bioRxiv - Bioengineering 2020Quote: ... tissue was incubated for at least 30 minutes in blocking solution (TNB, Perkin Elmer, with 0.1% Triton X-100) and if a mouse primary ...
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bioRxiv - Neuroscience 2021Quote: ... incubated with 3% of H2O2 for 30 minutes at RT and blocked 1 hour RT with NEN blocking buffer prepared according to the manufacturer’s protocol (FP1012 PerkinElmer). AntiDIG antibody conjugated to HRP (1:2000 ...
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bioRxiv - Neuroscience 2021Quote: ... incubated with 3% of H2O2 for 30 minutes at RT and blocked 1 hour RT with NEN blocking buffer prepared according to the manufacturer’s protocol (FP1012 PerkinElmer). AntiDIG antibody conjugated to HRP (1:1500 ...
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bioRxiv - Genetics 2019Quote: ... The slides were blocked in TNB buffer (0.1 M Tris-HCl pH 7.5, 0.15 M NaCl, 0.5% Blocking Reagent; PerkinElmer, Waltham, MA, USA) at RT for 45 min ...
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bioRxiv - Developmental Biology 2020Quote: ... incubated 1h in blocking buffer and then incubated overnight with an anti-DNP-POD antibody (1:1000, Perkin Elmer). The next day embryos were washed in PBS-Tween ...
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bioRxiv - Cancer Biology 2023Quote: ... Dehydrated coverslips were overlaid with 0.3 μg/ml TAMRA–OO-(CCCTAA)3 or 0.3 μg/mL Alexa 488–OO-(CCCTAA)3 telomeric PNA probe (Panagene) in PNA hybridization solution (70% deionized formamide, 0.25% (v/v) NEN blocking reagent (PerkinElmer), 10 mM Tris–HCl ...
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bioRxiv - Biochemistry 2021Quote: ... Substrate peptides or nucleosomes were mixed in a solution containing 9µM tritiated S-Adenosyl Methionine (SAM, Perkin Elmer) cofactor ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 μL immunoprecipitated enzyme were mixed with 300 μM synthetic peptide and 50 μM [14C]-acetyl-CoA (PerkinElmer) in acetylation buffer (50 mM HEPES pH 8.5 ...
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bioRxiv - Neuroscience 2022Quote: ... 2.7 mM KCl with 0.2% Triton X-100 and then blocked by shaking for 60 min in TSA Blocking Reagent (Perkin Elmer). Sections were left shaking for 1-3 nights at 4°C in primary antibodies ...
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bioRxiv - Biochemistry 2021Quote: In vitro peptide-based Aur A assays were carried out using a Caliper LapChip EZ Reader platform (Perkin Elmer), which monitors real-time phosphorylation-induced changes in the mobility of a fluorescently labelled Kemptide peptide substrate (5’-FAM-LRRASLG-CONH2 ...
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bioRxiv - Developmental Biology 2021Quote: ... Heat mediated antigen retrieval was used to process sections (Vector, H-3300) and then samples were blocked for 1 hour in blocking buffer at room temperature (PerkinElmer, FP1020). Primary antibodies diluted in blocking buffer were incubated with samples overnight at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were rinsed 3 times for 2 min in PBS-Tx and incubated in tyramide signal amplification (TSA) blocking reagent (PerkinElmer, FP1012) in PBS-Tx (TSA-block ...
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bioRxiv - Neuroscience 2021Quote: ... Then 5 µl of each diluted cell lysate was mixed with 15 µl of cell lysis buffer containing 0.33 mM ATP and 5 µM ProfilerPro Kinase Peptide Substrate 11 5-FAM-KKLNRTLSVA-COOH (PerkinElmer, U.S.A.) in the presence or absence of 0.66 µM CaM (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... A LANCE Ultra TR-FRET kinase assay was used for measurements of enzyme activities that included an-Ulight labeled (Thr-1342) peptide substrate (catalog #TRF0130-M, PerkinElmer) and a Europium-labeled anti-phospho peptide antibody (catalog # TRF0218-M) ...
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bioRxiv - Biochemistry 2020Quote: ... was incubated with 5 μL of biotinylated peptides at 100 nM for 30 min at room temperature in the epigenetics assay buffer (PerkinElmer AL008 ...
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bioRxiv - Neuroscience 2022Quote: ... were challenged with synthetic peptides reconstituted in DMEM/BSA medium and luminescence was measured on a MicroBeta LumiJet luminometer (PerkinElmer). Luminescence was measured for 30 s at a wavelength of 469 nm ...
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bioRxiv - Biochemistry 2020Quote: ... the sections were washed and blocked with TN with 0.5% (w/v) Blocking Reagent TSA fluorescein system (Perkin Elmer, Waltham, MA, USA) for 30 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... the PFA-fixed sections were blocked and permeabilized in TNBT (0.1 M Tris, pH 7.4, 150 mM NaCl, 0.5% blocking reagent from Perkin Elmer, 0.5% Triton X-100) for 4h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... or 3.3 mM (Figure 5-figure supplement 1 and 8) ATP and 5 µM ProfilerPro Kinase Peptide Substrate 11 5-FAM-KKLNRTLSVA-COOH (PerkinElmer, U.S.A.), in the presence or absence of 0.66 µM CaM (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... Radioactive counts from the media that remain soluble after TCA precipitation (peptide fragments) and pellets from the cells (total non-degraded cell proteins) were measured in a TopCount radioactivity detector (Perkin Elmer). A “protein degradation index” was calculated as the ratio between the counts detected in the soluble media and the counts detected in the pellet cell fraction (82).
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bioRxiv - Cell Biology 2019Quote: ... GST-Aq-NF-κB or GST-Aq-NF-κB-ALA C-terminal peptides and 5 μCi [γ-32P] ATP (Perkin Elmer) in kinase reaction buffer (25 mM Tris-HCl ...