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Citations for PerkinElmer :
51 - 100 of 1046 citations for NADH dehydrogenase ubiquinone 1 alpha subcomplex assembly factor 3 NDUFAF3 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated with TSA plus Cyanine-3 reagent (1:2000, NEL760001KT, Perkin Elmer) for 10 to 15 min at RT in accordance with the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... Insulin was quantified using the Insulin (human) AlphaLISA Detection Kit and the EnSpire Alpha Plate Reader (both Perkin Elmer) based on 1:10 and 1:200 dilutions for supernatant and insulin content ...
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bioRxiv - Cell Biology 2024Quote: ... Fresh mouse cardiac biopsies (1 biopsy ∼1-3 mg/well) were distributed in a 96-well white microplate (OptiPlate-96; PerkinElmer) and incubated or not (basal ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1:400 anti-6xHis terbium antibody (PerkinElmer #61HI2TLF) in 10 uL of buffer containing 20 mM HEPES ...
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bioRxiv - Cancer Biology 2022Quote: ... and 1:400 anti-6xHis terbium antibody (PerkinElmer #61HI2TLF) in 10μL of buffer containing 20mM HEPES ...
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bioRxiv - Neuroscience 2020Quote: ... Extracellular culture media were collected in order to dose secreted Aβ using Alpha-LISA assays (AlphaLISA Amyloid β1-X Kit, AL288C, PerkinElmer) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... with fluorescence detected on intensity (485 nm excitation and 530 nm emission) measured by an EnSpire Alpha Multimode Plate Reader (PerkinElmer).
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bioRxiv - Biochemistry 2023Quote: ... to a fixed concentration of target (e.g., biotinylated PTM-defined nucleosome) with the appropriate Alpha Donor and Acceptor beads (Perkin Elmer) (Jain et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... was done using the same procedure for the cold transcription except for the use of 0.5 µl alpha-32P-labeled UTP (3000 Ci/mmol-10 mCi/ml) (Perkin Elmer) and 0.8 mM UTP (instead of 2 mM) ...
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bioRxiv - Cancer Biology 2024Quote: ... was added into each well and the luciferase intensity in each well was immediately measured using an Alpha Microplate reader (PerkinElmer). The viability of MOLM-13luc cells was calculated after normalizing to the DMSO control and the dose-response curve of quizartinib was generated by GraphPad Prism 8.0.1.
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bioRxiv - Developmental Biology 2021Quote: ... cyanine 3 (PerkinElmer #NEL744E001KT), and cyanine 5 (PerkinElmer #NEL745E001KT) ...
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bioRxiv - Cancer Biology 2021Quote: ... For each PI3K isoform a 3 µL mixture (1:1) of enzyme/lipid substrate solution was added to a 384 well white assay plate (Perkin Elmer #6007299) containing 50 µL of test compound or DMSO only for untreated controls ...
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bioRxiv - Molecular Biology 2021Quote: ... Plates were read at 450 nm and readings were processed to observe binding patterns of tested molecules (Perkin Elmer, Enspire Alpha). For every experiment ...
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bioRxiv - Molecular Biology 2023Quote: ... Reactions were initiated by the addition of GTP to a final concentration of 40 uM containing a trace amount of [alpha-32P]-GTP (Perkin Elmer) and incubated for 20 min at 30°C ...
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bioRxiv - Genomics 2020Quote: ... and 1 μCi of [α-32P]-3’-deoxyadenosine 5’-triphosphate (cordycepin 5’-triphosphate, Perkin Elmer Life Sciences) in 1× terminal deoxynucleotidyl transferase buffer (New England Biolabs) ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 ppm and 1.5 ppm (Multi-element calibration standard 3 for ICP, 10µg/ml, PerkinElmer, L2-20MKBY1). The metal homeostasis analysis is done in Perkin-Elmer Optima 700 DV ICP optical emission spectrometer version 5.0 ...
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bioRxiv - Cell Biology 2021Quote: ... 41.3 Ci mmol−1) and radiocarbon-labelled serotonin binoxalate (14C-serotonin; 54.0 mCi mmol−1) were purchased from Perkin Elmer (Waltham, Massachusetts, USA). Serotonin creatinine sulphate ...
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bioRxiv - Biochemistry 2022Quote: ... 1 nM LANCE Ultra Europium anti-rabbit IgG antibody (PerkinElmer AD0082), and 50 nM LANCE Ultra Ulight-Streptavidin (PerkinElmer TRF0102 ...
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bioRxiv - Neuroscience 2023Quote: ... and HRP-labeled goat anti-rabbit antibody (1:500, PerkinElmer, NEF812001EA) for secondary detection ...
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bioRxiv - Neuroscience 2023Quote: ... cAMP levels were measured with an Amplified Luminescent Proximity Homogeneous Assay (ALPHA) Screen cAMP assay kit as instructed by the manufacturer (PerkinElmer, Waltham, MA).
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bioRxiv - Cancer Biology 2023Quote: ... and the plate was incubated in the dark for 2 h and then read in an EnSpire Alpha multimode plate reader (PerkinElmer, Waltham, MA, USA).
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bioRxiv - Genomics 2020Quote: ... These steps were repeated starting from blocking for the second staining with primary antibody rat anti-CD8a 1:300, secondary antibody goat anti-rat (MP-7444, Vector) and opal 690 (fp1497, PerkinElmer) and the third staining with rat anti-CD45 1:1,000 ...
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SPNS1 is required for the transport of lysosphingolipids and lysoglycerophospholipids from lysosomesbioRxiv - Biochemistry 2022Quote: ... and lower phase (containing [3-3H]-sphingosine, [3-3H]-ceramides, [3-3H]-SM) were quantified by a scintillation counter (Perkin Elmer Tri-Carb Liquid Scintillation Analyzer).
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bioRxiv - Biochemistry 2020Quote: ... 3 μL γ32P-dATP (Perkin Elmer), and 5 μL H2O for 1 hour with shaking (1250 rpm ...
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bioRxiv - Microbiology 2021Quote: ... 3 pM GTP α-32P (PerkinElmer), 10 Units of VCE and incubated 30 min at 37°C.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 0.5 ml Optiphase HISAFE 3 (PerkinElmer) was incubated with the filter overnight before liquid scintillation counting with MicroBeta JET (PerkinElmer) ...
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bioRxiv - Genomics 2022Quote: ... and TSA Plus Cyanine 3 (PerkinElmer). After the last washing step ...
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bioRxiv - Neuroscience 2021Quote: ... Oocytes were lysed with 1M NaOH and 1%SDS before the addition of 3 mL scintillant (Optiphase HisSafe, PerkinElmer), and l-[3H]glutamate transport was measured using a MicroBeta TriLux scintillation counter (PerkinElmer).
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bioRxiv - Cancer Biology 2021Quote: ... Slides were washed 3 times in PBS and incubated with tyramide Cy3 1:150 in amplification buffer (Perkin Elmer) for 10 min ...
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bioRxiv - Cell Biology 2020Quote: Fully differentiated C2C12 MS2-CAS myotubes were starved in serum-and glucose-free DMEM for 2 hrs before a 3-hr incubation in low-glucose DMEM/fatty acid-free BSA 0.2% supplemented with radiolabeled glucose (Glucose, D-[3-3H]; 1 μCi/ml; PerkinElmer). Then ...
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Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: ... Activity of the SMG1i against other PI3 kinase family members (PI3Kα, PI3Kβ, PI3Kγ, PI3Kδ, MTOR) was evaluated using Amplified Luminescent Proximity Homogeneous Assay (Alpha) Screen assays (Perkin Elmer and Echelon Biosciences). PI3K or mTOR protein (produced in Sf9 cells ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... We next added 0.8 μl of FLAG-GST-transcription factor proteins to 384-well AlphaPlates using a NanoHead (PerkinElmer) and a Janus Workstation (PerkinElmer) ...
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bioRxiv - Neuroscience 2020Quote: ... 3 and 6 hr later cells were labeled for 5 min with 1 mCi/ml 35S Protein Labeling Mix (PerkinElmer). Lysates from 25-ml culture triplicate samples were analyzed by SDS-PAGE and autoradiography.
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bioRxiv - Neuroscience 2021Quote: ... The signal of CB1 receptor hybridization was revealed by a TSA reaction using cyanine 3 (Cy3)-labeled tyramide (1:100 for 10 minutes, NEL744001KT PerkinElmer). After several washes ...
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bioRxiv - Microbiology 2022Quote: ... 48 hpi liver-stage cultures were washed with PBS and lysed in Nano-Glo Luciferase Assay buffer containing Nano-Glo Luciferase Assay substrate diluted 1:200 and luminescence was measured after 3 minutes on an IVIS Specturum CT (PerkinElmer). To monitor merosome detachment at 65 hpi ...
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bioRxiv - Cell Biology 2022Quote: ... Hybridization signals were detected by a chromogenic reaction using red chromogen dilution 1:3000 (PerkinElmer TSA Plus Cyanine 3 System). RNA-staining signal was identified as red punctate dots and clusters ...
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bioRxiv - Systems Biology 2024Quote: ... Finally 100 μl sample was added to scintillation vials containing 3 mls scintillant (OptiPhase HiSafe 3, Perkin Elmer). β-radioactivity was measured with Tri-Carb 4910TR liquid scintillation counter.
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bioRxiv - Biochemistry 2023Quote: [125 I]-Bolton-Hunter labeled Transforming Growth Factor- β1 (Human, Recombinant) (catalog NEX267) was obtained from Perkin Elmer (Waltham, MA) This product was however discontinued in 2019 ...
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bioRxiv - Systems Biology 2020Quote: ... Cy3-TSA (3 min; Perkin Elmer NEL744001KT), Cy5-TSA (7 min ...
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bioRxiv - Microbiology 2021Quote: ... in XIC-3 animal isolation chamber (PerkinElmer) that provided biological isolation of anesthetized mice or individual organs during the imaging procedure ...
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bioRxiv - Immunology 2021Quote: ... in XIC-3 animal isolation chamber (PerkinElmer) that provided biological isolation of anesthetized mice or individual organs during the imaging procedure ...
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bioRxiv - Cell Biology 2020Quote: ... 3 μL 35S-labeled methionine (PerkinElmer NEG709A500UC), 2.5 μL recombinant protein (~450ng ...
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bioRxiv - Microbiology 2022Quote: ... in XIC-3 animal isolation chamber (PerkinElmer) that provided biological isolation of anesthetized mice or individual organs during the imaging procedure ...
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bioRxiv - Cell Biology 2024Quote: ... TSA Plus Cyanine 3 System (PerkinElmer NEL744001KT) was used to amplify signals generated using antibodies for RBPJ ...
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bioRxiv - Neuroscience 2020Quote: ... was used as a secondary fluorophore for C1 probes (Gtpbp2, Slc7a1, Chac1) and TSA® Plus Cyanine 3 (PerkinElmer, 1:2,000) was used as a secondary fluorophore for C2 probes (Gtpbp1 ...
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Peripheral neuropathy linked mRNA export factor GANP reshapes gene regulation in human motor neuronsbioRxiv - Neuroscience 2021Quote: ... were hybridized for 2 h at 40°C followed by signal amplification and developing the HRP channel with TSA Plus fluorophore Cyanine 3 (1:1500) (NEL744001KT, Perkin Elmer) according to the manual ...
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bioRxiv - Molecular Biology 2023Quote: ... Fluorescently labeled RNA was obtained by replacing 1/4 volume of CTP with Cyanine 3-CTP (Cy3-CTP) or Cyanine 5-CTP (Cy5-CTP) (PerkinElmer NEL581001EA) in the in vitro transcription reaction.
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bioRxiv - Cell Biology 2024Quote: ... After two washes of 5min in 1X RNAscope wash buffer slides were incubated with 1:1500 Cyanine 3 amplification reagent (PerkinElmer, #FP1170012UG), or 1:1000 Cyanine 5 amplification reagent (PerkinElmer ...
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bioRxiv - Microbiology 2023Quote: ... Secondary antibodies used were 1:10,000 of HRP-labeled α-mouse (for Flag) (PerkinElmer) or α-rabbit (PerkinElmer ...
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bioRxiv - Immunology 2024Quote: ... 1:20000 Eu3+ cryptate conjugated to a His6-specific antibody (HTRF donor, PerkinElmer AD0402) and 1:500 Streptavidin-XL665 (HTRF acceptor ...