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Citations for PerkinElmer :
51 - 100 of 545 citations for Insulin like growth factor binding protein 6 IGFBP 6 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Number of basal and suprabasal cells in each clone was counted using Volocity 6 Image Analysis software (PerkinElmer).
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bioRxiv - Neuroscience 2020Quote: HUVECs were incubated for 6 hours in EGM-2 containing 0.4 μCi/ml [5-3H]-D-glucose (PerkinElmer). Supernatant was transferred into glass vials containing perchloric acid and sealed with rubber stoppers ...
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bioRxiv - Cell Biology 2022Quote: ... Hepatocytes were seeded into 6-well (300,000 per well) and 384-well microplates (1,000 per well, CulturPlate, PerkinElmer) precoated with collagen (rat tail ...
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bioRxiv - Physiology 2021Quote: ... Plasma insulin concentrations (from tail vein samplings) were determined using an ultrasensitive mouse insulin AlphaLisa detection kit (cat number AL204C) from Perkin Elmer.
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bioRxiv - Biochemistry 2020Quote: ... Purified hDGAT1 (with his tag) was absorbed onto Copper HIS-Tag PVT beads (Perkin Elmer, RPNQ0095) and incubated with [3H]-Acetyl-CoA (ARC ...
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bioRxiv - Genomics 2021Quote: ... Insulin was quantified using the Insulin (human) AlphaLISA Detection Kit and the EnSpire Alpha Plate Reader (both Perkin Elmer) based on 1:10 and 1:200 dilutions for supernatant and insulin content ...
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bioRxiv - Bioengineering 2022Quote: Saturation assays for binding of K1K1H6 to recombinant MET-Fc protein were performed in 384-well microtiter plates (OptiPlate™-384, PerkinElmer© ...
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bioRxiv - Physiology 2020Quote: ... glycosylphosphatidylinositol-anchored high-density lipoprotein binding protein 1 (GPIHBP1) knock out mice were orally gavaged with 100 μCi of [9,10-3H(N)]-Triolein (Perkin Elmer, NET431001MC) suspended in olive oil ...
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bioRxiv - Cell Biology 2019Quote: ... with a 60X 1.42 NA oil-immersion objective coupled to an Ultraview Vox spinning disk confocal system running Volocity 6 (PerkinElmer). Images were captured with a EMCCD camera (C9100-13 ...
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bioRxiv - Bioengineering 2019Quote: ... and 6 post-infection was performed via radiographs with fluorescent overlays using the IVIS Lumina XRMS II system (PerkinElmer).
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bioRxiv - Biochemistry 2019Quote: ... Plates were then washed 6 times with PBST and buffer containing PBS and 0.2 µg/ml Eu3+-streptavidin (PerkinElmer) and incubated at room temperature for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: High-binding 384-well SpectraPlates (Perkin Elmer) were coated with proteins of interest at a concentration of 1 µg/ml in sterile PBS and at a volume of 20 µl/well ...
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bioRxiv - Neuroscience 2021Quote: High-binding 384-well SpectraPlates (Perkin Elmer) were coated with mouse mAb 4-10-C9 at a concentration of 1 µg/mL in sterile PBS and at a volume of 20 µl/well ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2.3±0.64 pmol/mg protein (Bmax for β 2AR) and binding affinities for the radioligand [³H]CGP12,177 (specific activity 51 Ci/mmol, PerkinElmer, Rodgau, Germany) of 0.070 nM (KD for β1AR ...
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bioRxiv - Cancer Biology 2023Quote: ... Europium labelled anti-His (Perkin Elmer # AD0110) and XL665 labelled streptavidin (CIS Bio 610SAXAC ...
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bioRxiv - Molecular Biology 2019Quote: Cells were incubated with complete growth media supplementing with EasyTagTM Express Protein Labeling Mix containing both [35S]-L-methionine and [35S]-L-cysteine (PerkinElmer) at 10 μCi/ml for 30 min at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... the two replicates of the 6 h treatment time point for the H1299 cell line were acquired on an equivalent Opera Phenix imaging system (PerkinElmer). Quantitative analysis of the data was carried out using the metrics and analysis methods detailed below ...
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bioRxiv - Molecular Biology 2019Quote: ... For micro-CT irradiation cells in 6 cm culture dishes or in 1.5 ml microcentrifuge tubes were scanned (Quantum FX, Perkin Elmer) in the low resolution mode (73 mm f.o.v. ...
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bioRxiv - Biochemistry 2021Quote: ... and 640 μM) were added along with 10 μM of non-radiolabeled UTP plus 6 mCi of [α32P]-UTP (PerkinElmer). Upon addition of nucleotides ...
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bioRxiv - Immunology 2020Quote: ... during the final 6 hours (murine MLR) or 15 hours (human MLR) of culture was measured by a TopCount (PerkinElmer).
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bioRxiv - Cell Biology 2019Quote: GBM2 cells were plated at 2000 cells/well and exposed to Prestwick compounds (3 µM; Table 6) for 3 days in 384-well optical bottom assay plates (PerkinElmer). Cells were then fixed and stained with rabbit polyclonal anti-H3K27ac and mouse monoclonal anti-H3K27me3 antibodies followed by AlexaFluor-488- or AlexaFluor-555-conjugated secondary antibodies ...
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bioRxiv - Cancer Biology 2021Quote: 4T1 cells were cultured for 6 h in RPMI medium containing 0.4 μCi ml−1 [5-3H]D-glucose (Perkin Elmer) after which the supernatant was transferred into glass vials sealed with rubber stoppers ...
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bioRxiv - Microbiology 2022Quote: ... maintained at 200 °C and with N2 as the gas vector (flow rate of 6 mL·min-1) with a flame ionization detector (FID) maintained at 280 °C (PerkinElmer, USA)
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bioRxiv - Microbiology 2022Quote: ... OD600 and luminescence measurements were taken either 1-hour post-resuspension or over 6 hours starting at 0.5-hour post-resuspension using the Victor X3 2030 multilabel plate reader (Perkin Elmer). Luminescence was measured in counts per second (CPS ...
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bioRxiv - Immunology 2024Quote: ... Tumor engraftment was confirmed on day 6 via intraperitoneal injection of IVISbrite D-Luciferin Potassium Salt Bioluminescent Substrate (XenoLight, PerkinElmer), followed by bioluminescent imaging (BLI ...
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bioRxiv - Cancer Biology 2024Quote: ... Eµ-Myc lymphoma cells grown in 6-well plates were spiked with 0.2 μCi/mL [1-14C]-acetate (PerkinElmer; NEC084H001MC) for the final 4 h of the experimental period ...
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bioRxiv - Immunology 2024Quote: ... [3H]-thymidine was added at 0.5μl (∼0.5 μCi) per well and plates incubated for 6 h before cells were harvested to UniFilter plates (Perkin Elmer) using a Tomtec 96 Harvester ...
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bioRxiv - Physiology 2023Quote: ... and blood was collected for the measurement of both glucose (Accu-Check) and insulin levels (Insulin AlphaLISA detection kit, Perkin Elmer, USA).
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bioRxiv - Physiology 2023Quote: ... Perfusates were continuously and automatically collected into 96-well plates for the detection of insulin (Insulin AlphaLISA Detection Kit, Perkin Elmer, USA). Insulin measurement was performed according to the manufacturer’s instructions.
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bioRxiv - Systems Biology 2022Quote: T-REx HEK293 cells were seeded in 384-well PhenoPlates (PerkinElmer #6057302) manually coated with collagen I in T-REx base media supplemented with doxycycline (1 ug/mL) ...
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bioRxiv - Systems Biology 2022Quote: T-REx HEK293 cells were seeded in 96-well PhenoPlates (PerkinElmer #6055302) manually coated with collagen I in T-REx base media supplemented with doxycycline (1 ug/mL) ...
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bioRxiv - Bioengineering 2022Quote: ... HEK293-ACE2 cells were seeded in 384-well white plates (PerkinElmer, USA), allowed to attach overnight followed by addition of pseudoviral particles in the presence or absence of varying concentrations of GRFT ...
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bioRxiv - Neuroscience 2021Quote: ... A high-binding 96-well OptiPlate (Perkin Elmer) was coated with anti-TRKB antibody incubated in carbonate buffer (57.4 mM sodium bicarbonate and 42.6 mM sodium carbonate ...
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bioRxiv - Microbiology 2019Quote: ... Collected cell batches on polycarbonate filters were directly transferred into 6 ml scintillation vials and mixed with 5 ml UltimaGold XR (Perkin Elmer) scintillation cocktail ...
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bioRxiv - Biochemistry 2021Quote: ... were based on an initial output from LigPlot+48 and the chemical reaction pathway (Supplementary Fig. 6) was created in ChemDraw (PerkinElmer Informatics).
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bioRxiv - Cell Biology 2021Quote: ... 500nl of 2 µM siRNAs (20nM final concentration) were distributed on 6 different 384-well imaging plates (CellCarrier Ultra, Perkin Elmer), as described in Table 3 ...
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bioRxiv - Immunology 2019Quote: ... After 4-6 washes with 1x TBST the membrane was treated with chemiluminescent substrate Western Lightning ECL Pro (Perkin Elmer; NEL120001EA) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... brain and testis tissue samples from hACE2 transgenic mice challenged intranasally with SARS-CoV-2-nLuc (1 x 105 FFU; 6 dpi) were imaged after necropsy using bioluminescence imaging (IVIS, Perkin Elmer), pruned to isolate regions with high nLuc activity and immediately pre-fixed with 3 % glutaraldehyde ...
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bioRxiv - Immunology 2021Quote: M-CSF-dependent mouse bone marrow derived macrophages isolated from the long bones of C57BL/6 mice were cultured for 48 hours on devitalized bovine bone discs (0.2 mm x 6 mm) labelled with fluorescently conjugated inert bisphosphonate (Osteosense, Perkin Elmer, NEV10020EX) either in the absence or in the presence of mature osteoclasts that had been differentiated for 5-days under pro-osteoclastogenic conditions i.e ...
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bioRxiv - Cancer Biology 2021Quote: ... sections were stained for NICD1/KRT14/DNA or NICD1/Ki67/DNA and epithelial thickness measured and cell counting performed using Volocity 6 software (Perkin Elmer). Cell density was measured by counting the number of nuclei identified across the width of the epithelium on high resolution images of cryosections stained with DAPI and keratinocyte markers such as KRT4 and ITGA6 and dividing this number by the measured surface area.
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bioRxiv - Cancer Biology 2021Quote: ... Contiguous areas were analyzed for presence or absence of NOTCH1 or nuclear NICD1 staining and their length along the section measured using Volocity 6 software (Perkin Elmer). For morphological analysis of NICD1 and positive negative areas (Extended data Fig.1 f-h) ...
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bioRxiv - Cancer Biology 2021Quote: ... stained sections were imaged using Leica TCD SP8 confocal microscope at 40x objective as described in ‘confocal microscopy’ and analyzed with Volocity 6 software (Perkin Elmer). For counting Ki67 positive basal cells in the aged tissue (Fig.5d) ...
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bioRxiv - Cell Biology 2020Quote: ... subconfluent HDLECs cultured in 12-well plates were incubated with 1 ml per well EBM2 medium (containing appropriate amounts of serum and supplement) with [6-14C]-glucose (Perkin Elmer) for 6 hours ...
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bioRxiv - Immunology 2022Quote: ... Paracellular permeability was determined by measuring mucosal-to-serosal flux (%flux/cm2/hour) of the small inert probe 51Cr-EDTA (360 Da; 6 μCi/mL) (Perkin Elmer), by taking an initial radiolabelled sample of mucosal buffer and then samples from the serosal compartment every 30 minutes for 2 hours ...
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bioRxiv - Microbiology 2024Quote: ... The filters were rinsed with 35 ‰ sodium chloride (NaCl) solution [50] and transferred into 7 mL scintillation vials containing a 6 mL scintillation cocktail (Ultima Gold ™ Cocktail, PerkinElmer). The radioactivity of the filters was determined using a Tri-Carb 3110TR liquid scintillation counter [51] ...
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bioRxiv - Immunology 2024Quote: ... Pups were injected intraperitoneally with D luciferin (150 mg/kg) at 6 hours after LNP injection and imaged using an IVIS Lumina system (Perkin Elmer). To evaluate the in vivo response to CGT ODNs ...
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bioRxiv - Developmental Biology 2023Quote: ... Paracellular permeability was assessed by mucosal-to-serosal flux (%flux/cm2/hr) of the small inert probe 51Cr-EDTA (360 Da; 6 μCi/mL) (Perkin Elmer), by taking an initial radiolabelled sample of mucosal buffer and then samples from the serosal compartment every 30 minutes for 2 hours ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... was added to the cell suspension and cells were plated (2,500 cells, 6 μL) in a 384-well white opaque microtiter plate (OptiPlates, Perkin Elmer, 6007299). Cells were incubated with serial dilutions (3 μL ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 µl of the virus or virus-antibody complex was added to each well in triplicates and allowed to incubate for 6 hrs before the cells were lysed with 50 µl/well of steadylite plus luciferase reagent (Perkin Elmer; 6066751). The luciferase activity was quantified using a BMG PHERAstar FS plate reader.
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bioRxiv - Genetics 2020Quote: ... 50 U of DNA polymerase I (M0209S) and 30 μM of each dNTP including 6 μM of Fluorescein-12-dATP (Perkin Elmer, NEL465001EA) or 6 μM of Texas Red-5-dATP (Perkin Elmer ...