Labshake search
Citations for PerkinElmer :
51 - 100 of 230 citations for Gamma secretase subunit APH 1B APH1B Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... and the radioactivity (CPM) in each well was measured using a gamma counter (PerkinElmer 2480 Wizard2). The wells were washed thrice with 200µl of the Washing buffer during each washing step ...
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bioRxiv - Immunology 2024Quote: ... and radioactivity concentrations were measured with a well-type gamma-counter (Wallac 2480 wizard, Perkin Elmer) or a NaI crystal scintillation counter (Osprey ...
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bioRxiv - Systems Biology 2020Quote: ... FITC-TSA (2 min; Perkin Elmer NEL701A001KT), Cy3-TSA (3 min ...
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bioRxiv - Genomics 2020Quote: ... at 37°C for 1 hr and then labeled with 0.5μl of gamma 32P-ATP (PerkinElmer, BLU502A250UC) using 20U T4 polynucleotide kinase (NEB ...
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bioRxiv - Cell Biology 2021Quote: ... The radiation intensity of 64Cu signals was measured in a well gamma-counter (Wallach Wizard, PerkinElmer, USA).
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bioRxiv - Bioengineering 2022Quote: ... and radioactivity was measured on a Wizard 1470 Automatic Gamma counter (PerkinElmer Life Sciences, Wallac Oy, Finland). Radiation uptake is expressed as percentage of injected dose (% ID ...
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bioRxiv - Neuroscience 2022Quote: ... From these samples whole-blood and plasma activity were measured in a gamma counter (Wizard2, Perkin Elmer). The arterial input function was obtained by linear interpolation of the manual samples to match PET frames and multiplication with the average plasma-to-whole-blood ratio.
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bioRxiv - Pathology 2023Quote: ... The amount of radioactivity in the final pellets was measured with a WIZARD2 gamma counter (Perkin Elmer). All binding assays were replicated using two different BBMV preparations each with two to three technical replications ...
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bioRxiv - Immunology 2024Quote: ... the total incubated radioactivity CPM was measured in a gamma counter (PerkinElmer 1480 Wizard or 2480 Wizard2). The cell mixture was then microcentrifuged at 12,000g for 5 min (Eppendorf 5415C) ...
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bioRxiv - Physiology 2022Quote: ... EP3R immunoreactivity was visualized by reaction with FITC-conjugated tyramide (1:50; SAT701001KT; Tyramide Signal Amplification FITC Systems, PerkinElmer) for 3 min ...
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bioRxiv - Neuroscience 2021Quote: ... whole blood activity and following centrifugation also plasma activity were measured in a gamma-counter (Wizard2, Perkin Elmer). Whole blood activities were linearly interpolated to match PET frames and multiplied with the average plasma-to-whole-blood ratio ...
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bioRxiv - Cancer Biology 2020Quote: ... The bound radioactivity for these samples was determined by measuring gamma radiation (Perkin Elmer 2480 Wizard3, Shelton, CT). From the saturation studies ...
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bioRxiv - Neuroscience 2020Quote: Radioactivity in blood and plasma was measured with an auto-gamma counter (wizard 2480, PerkinElmer, Waltham, MA, USA). Plasma samples taken at 2 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the amount of radioactivity in blood and organs was measured using a gamma counter (Wizard2, PerkinElmer, Waltham, MA).
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bioRxiv - Immunology 2021Quote: ... The remaining cell-bound radioactivity was measured using a Wizard² 2480 gamma counter (PerkinElmer Inc., Waltham, MA, USA) and quantified as percentage of total added activity.
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bioRxiv - Immunology 2024Quote: ... and the fraction of displaced labeled peptide was quantified with a gamma counter (Perkin Elmer, Wallac 1272 CliniGamma). Binding curves were determined by plotting the amount of displaced labeled peptide vs the concentration of unlabeled competitor ...
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bioRxiv - Neuroscience 2024Quote: ... whole blood activity and plasma activity after centrifugation were measured in a gamma-counter (Wizard2, 3”; Perkin Elmer, USA). The whole blood curve was linearly interpolated and resampled to match the time points of the reconstructed fPET frames ...
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bioRxiv - Pathology 2020Quote: ... and the FITC fluorophore (Perkin Elmer cat. no. NEL741001KT). Slides were mounted in ProLong Gold Antifade Mountant with DAPI (Life Technologies ...
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bioRxiv - Bioengineering 2020Quote: ... The cells were lysed in 75 µl RIPA lysis buffer containing 0.5% NP-40 and the radioactivity was measured using a Gamma Scintillation Counter (Wallac 1470 (Perkin Elmer), Location) ...
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bioRxiv - Bioengineering 2024Quote: ... Cell pellets were then collected by centrifugation and radioactive uptake was measured using a gamma counter (Wizard Detector, Perkin Elmer). Uptake (%ID/million cells ...
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bioRxiv - Cell Biology 2020Quote: ... Radioactivity of both the supernatant and the cells was measured by the 2480 Wizard2 Automatic Gamma Counter (PerkinElmer, Waltham, MA, USA). Uptake values were adjusted for tracer decay and corrected for cell amount.
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bioRxiv - Cancer Biology 2021Quote: ... The lysed cells were transferred to vials and measured in a gamma counter (Wallac 1470 Wizard, Perkin Elmer, Waltham, MA, USA). Obtained cpm values were plotted with GraphPad Prism and the data fitted using nonlinear regression (one site - fit Ki ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed twice and cell-associated activity was subsequently measured in a well-type gamma-counter (Wallac 2480 wizard, Perkin Elmer). In a separate binding assay equimolar concentrations (1.97 pM ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The lysed cells were transferred to vials and measured in a gamma counter (Wallac 1470 Wizard, Perkin Elmer, Waltham, MA, USA). Obtained cpm values were plotted with GraphPad Prism 7 and the data were fitted using nonlinear regression (one site - fit Ki ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant and precipitate were separated and both fractions were counted in a gamma counter (Wizard 2 2480, Perkin Elmer, USA). Following this ...
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bioRxiv - Microbiology 2021Quote: ... 200 μL of each phase were collected and measured in a 2480 automatic Gamma counter Wizard 2 3” (PerkinElmer, Waltham, MA, USA). LogD value were calculated using Excel by dividing measured values of octanol by water and logarithmize the result ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Tissue distribution of injected materials was determined by measuring the radioactivity in the blood and other organs using a Wizard 2470 gamma counter (PerkinElmer, Waltham, MA). Tissue uptake was presented as percent injected dose normalized to the mass of tissue (%ID/g tissue).
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bioRxiv - Immunology 2024Quote: ... and the counts per minute (CPM) in the cell pellets were measured in a gamma counter (PerkinElmer 1480 Wizard or 2480 Wizard2). The binding specificity was expressed as the CPM ratio of TB to NSB ...
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bioRxiv - Developmental Biology 2024Quote: ... Another Tyramide amplification reaction (FITC) was performed for 90 minutes (PerkinElmer Life Science ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Timed blood (few drops drawn from the tail vein) and excreta collection was followed by weighing and γ–counting (PerkinElmer Wizard 2480 Automatic Gamma Counter, PerkinElmer, Waltham, MA). Values were corrected for decay and expressed as a percentage of injected dose per gram of tissue (%ID/gram).
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bioRxiv - Cancer Biology 2020Quote: ... 24 h post-labeling when secular equilibrium is reached between 225Ac and its daughter isotopes using a 2470 Wizard2 Gamma counter (Perkin Elmer, MA, USA) calibrated for the 225Ac and 213Bi emission spectra ...
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bioRxiv - Neuroscience 2020Quote: ... tyramide signal amplification was used (TSA-FITC) according to manufacturer’s instructions (PerkinElmer). DAPI (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... and the change in tryptophan fluorescence associated with the exchange of guanyl nucleotides within the α subunit of Gt was measured for 1200 s with a FL 6500 Fluorescence Spectrometer (PerkinElmer, Waltham, MA). Excitation and emission wavelengths were set at 300 nm and 345 nm ...
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bioRxiv - Cancer Biology 2024Quote: ... Fibronectin was detected using a Tyramide Signal Amplification (TSA-FITC) system (Perkin Elmer). Images were acquired on a Zeiss 710 using a 20x objective ...
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bioRxiv - Neuroscience 2020Quote: ... The FITC signals were visualized by tyramide signal amplification (TSA) fluorescence system (#NEL747A,Perkin Elmer) according to the manufacturer’s instructions using horseradish peroxidase (HRP)-IgG Fraction Monoclonal Mouse Anti-Fluorescein (#200-032-037,Jackson ImmunoResearch) ...
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bioRxiv - Neuroscience 2022Quote: ... Tyramide signal amplification was performed with 100 µL TSA® Plus FITC (1:60; PerkinElmer) per slide for 10 min in a dark chamber ...
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bioRxiv - Cancer Biology 2023Quote: ... were detected using a Tyramide Signal Amplification (TSA-FITC) system (Perkin Elmer, NEL741B001KT, 1:50).
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bioRxiv - Immunology 2021Quote: ... and FITC-dextran content was measured by fluorescence intensity using an EnVision Multilabel Plate Reader (PerkinElmer, Waltham, MA). Sampled volume was replenished with fresh DFM.
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bioRxiv - Molecular Biology 2023Quote: ... Slides were then washed three times with TNT buffer (TN buffer + 0.05% Tween-20) and incubated with FITC-tyramide diluted 1:100 in Amplification reagent (PerkinElmer) for 10 minutes at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were washed in PBSTw and then incubated in FITC-Tyramide (Perkin-Elmer) 1:100 in amplification reagent (Perkin Elmer), followed by several washes in PBSTw ...
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bioRxiv - Immunology 2024Quote: ... the distribution of fluorescein isothiocyanate (FITC) dextran in the mice was observed via a small animal imaging system (IVIS Spectrum, PerkinElmer). The peripheral blood of the mice was collected and centrifuged at 3000 r/min ...
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bioRxiv - Developmental Biology 2021Quote: ... the samples were immunostained as described above after detection of the Emi2 or cyclin B1 RNA probe with tyramide-FITC (PerkinElmer, Inc.) in in situ hybridization analysis ...
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bioRxiv - Neuroscience 2021Quote: ... The signal of CCK hybridization was revealed by a TSA reaction using fluorescein isothiocyanate (FITC)-labeled tyramide (1:100 for 10 minutes, NEL741001KT Perkin Elmer). After several washes ...
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bioRxiv - Microbiology 2022Quote: ... blood plasma collected from the tip of the tail vein was diluted with equal volume of PBS (pH 7.4) and analyzed for DX-4000-FITC concentration with a fluorescence spectrophotometer (HTS-7000 Plus-plate-reader; Perkin Elmer, Wellesley, Massachusetts, USA) at an excitation wavelength of 485 nm and emission wavelength of 535 nm ...
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bioRxiv - Immunology 2024Quote: ... After an additional 2 hours blood was collected and serum FITC-dextran levels were measured with a Victor V multiparameter plate reader (Perkin Elmer, Waltham, MA). Quantification of serum FITC-dextran levels was performed with the WorkOut 2.5 software by extrapolation against know concentrations of FITC-dextran standard.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... anti-cGMP antibody (PerkinElmer anti-cGMP antibody ...
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bioRxiv - Immunology 2024Quote: ... All antibodies were obtained from PerkinElmer Life Sciences except for the anti-FLAG-FITC conjugated antibody which was purchased from BioLegend ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were detected using HRP-linked secondary antibodies together with Western Lightning Plus-ECL (Perkin Elmer). Fiji (ImageJ ...
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bioRxiv - Neuroscience 2020Quote: ... antibodies plus Cy3-TSA (1:200, PerkinElmer). Sections were cover-slipped using Prolong Diamond Antifade Mounting media with DAPI (ThermoFisher ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Anti-cGMP antibody was obtained from PerkinElmer and GenScript ...