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Citations for PerkinElmer :
51 - 100 of 375 citations for Dengue Virus serotype 2 lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... was added to control wells before addition of 2-[3H]deoxyglucose (2-DG) (PerkinElmer). During the final 5 min 2-DG (0.25 μCi ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 mM DTT and 2 μCi/mL S-adenosyl-L-[methyl-14C] methionine (PerkinElmer). After 3h incubation ...
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bioRxiv - Bioengineering 2021Quote: ... A Wizard 2 (Perkin Elmer) gamma counter was used to quantify tracer uptake in organs ...
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bioRxiv - Physiology 2019Quote: ... [3H]2-DG (Perkin Elmer) was injected retro-orbitally in a bolus of saline containing 66.7 μCi/mL [3H]2DG corresponding to ~9-10 μCi/mouse (6 μL/g body weight ...
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bioRxiv - Cell Biology 2021Quote: ... 2’-14C]-Cystine (PerkinElmer, NEC854010UC) and washed three times in ice-cold PBS containing cold cystine (1 mM) ...
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bioRxiv - Cell Biology 2020Quote: ... Radioactivity was measured by liquid scintillation counting (100 μl of lysate in 3.9 ml of Emulsifier-Safe, Perkin Elmer).
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bioRxiv - Biochemistry 2021Quote: ... The supernatant and the cell lysate were added into separate scintillation tubes containing Ultima Gold LSC cocktail (Perkin Elmer) and the radioactivity was measured by a TriCarb 3100TR liquid scintillation counter (Perkin Elmer) ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were incubated for 1 hour at room temperature before FRET was detected using an Envision plate reader (PerkinElmer). Emission of Lumi4TM-Tb cryptate was detected at 620 nm and emission of d2-conjugated IP1 at 665 nm ...
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bioRxiv - Microbiology 2023Quote: ... Reactions contained 10 μL lysate with 250 ng of pcDNA T7 expression plasmid and 0.5 μL [35S] methionine/cysteine (PerkinElmer). Reactions were incubated at 30°C for 40 minutes chasing with 2 μL of 50 mg / mL unlabelled methionine/cysteine ...
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bioRxiv - Microbiology 2022Quote: ... Reactions contained 10 μL rabbit reticulocyte lysate with 500 ng of pcDNA T7 expression plasmid and 0.5 μL [35S] methionine (PerkinElmer). Reactions were incubated at 30°C for 40 minutes before being chased with 2 μl of 50 mg / mL unlabelled methionine ...
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bioRxiv - Cell Biology 2023Quote: ... by incubating 200 ng of DNA with 7 μL of TNT-T7 reticulocyte lysate mix and 0.5 µL of 35S-labelled methionine (NEG709A500UC, PerkinElmer) for 1 h at 30 °C at 600 rpm ...
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bioRxiv - Cell Biology 2023Quote: ... aliquots of derivatized lysates containing 15 µg of protein were spotted on a Whatman Protran nitrocellulose membrane (Perkin Elmer). Blots were blocked as above ...
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bioRxiv - Pathology 2024Quote: ... The fluorescence intensity (FI) of the medium and lysate was measured using an enzymatic calibrator (Perkin Elmer, Massachusetts, USA) at 469 nm excitation wavelength and 537 nm emission wavelength ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μCi of 2-Deoxy-D-[1-2-3H] glucose (PerkinElmer, Boston, MA, USA # NET328A001MC) and 10μM non-radioactive 2-DG (Sigma Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mL of each component was added to 2 mL Ultima Gold scintillation fluid (PerkinElmer), and radioactivity counted on the MicroBeta2 scintillation counter (PerkinElmer) ...
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bioRxiv - Cancer Biology 2021Quote: ... 30 ug of protein lysate was resolved by SDS-PAGE and transferred onto a Protran pure 0.2 micron nitrocellulose (Perkin Elmer). The membranes were incubated for 1h in 5% skimmed milk and probed with specific primary antibodies (Table S1 ...
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bioRxiv - Genomics 2019Quote: ... cell lysates were prepared with Passive Lysis Buffer and Luciferase activity was measured on a VICTOR X3 plate reader (PerkinElmer). Luciferase assay values are represented in fold change of Fluc (pGL4.23 plasmid ...
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bioRxiv - Immunology 2020Quote: ... luciferase activities of 150 μl lysate transferred from each well to a 96-well black solid plate were measured by a luminometer (PerkinElmer). The 50% inhibitory dose (ID50 ...
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bioRxiv - Immunology 2021Quote: ... cell lysate was loaded onto a 96-well plate (Grenier-BioOne) and mixed with MICROSCINTTM-20 scintillation fluid (Perkin Elmer). Samples were then incubated at room temperature for 2 hours and incorporated radioactivity analyzed on a 1450 MicroBeta TriLux Microplate Scintillation and Luminescence counter (Perkin Elmer).
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bioRxiv - Genomics 2022Quote: ... 20 µl of the lysate was transferred in duplicate in a well of an OptiPlate-96 HB (PerkinElmer, Waltham, MA) and 100 µl of Luciferase Assay Reagent II was added in each well ...
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bioRxiv - Microbiology 2023Quote: ... and the infectivity was determined by measuring the relative luciferase units (RLU) in the cell lysates using the Britelite plus system (PerkinElmer). Viral infectivity was considered positive if the RLU value was at least 5-fold higher than the background RLU value in the parental cell line ...
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bioRxiv - Cell Biology 2023Quote: ... 50 µ g of lysate was used for the assessment of ATP concentration using an ATP Determination Kit (ATP lite assay, PerkinElmer) as described earlier (Miyoshi et al. ...
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bioRxiv - Microbiology 2023Quote: ... and the infectivity was determined by measuring the relative luciferase units (RLU) in the cell lysates using the Britelite plus system (PerkinElmer). Viral infectivity was considered positive if the RLU value was at least 5-fold higher than the background RLU value in the NP-2 parental cell line ...
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bioRxiv - Microbiology 2023Quote: ... The infectivity in each well was determined by measuring the relative luciferase units (RLU) in the cell lysates using the Britelite plus system (PerkinElmer). The percentage of inhibition was determined by comparing the infectivity with positive control wells without drug inhibition.
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bioRxiv - Microbiology 2024Quote: ... and the infectivity was determined by measuring the RLUs in the cell lysates using the Britelite plus system (PerkinElmer, USA). Viral infection was considered positive if the RLU value was more than 5-fold higher than the background RLU value in parental NP-2 cell line ...
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bioRxiv - Microbiology 2024Quote: ... The infectivity in each well was determined by measuring the RLU in the cell lysate using the Britelite plus system (PerkinElmer). The percentage of Maraviroc inhibition was determined by comparing the RLU values with the positive control wells without Maraviroc inhibition ...
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bioRxiv - Immunology 2020Quote: ... 2 × 106 treated cells were incubated with 2 MBq of Na2 CrO4 (#NEZ030001MC, PerkinElmer, Waltham, MA) for 2 hours at 37 °C in a 5% CO2 atmosphere and were used as target cells ...
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bioRxiv - Molecular Biology 2020Quote: ... ~2 nM [α-32P]-ATP (PerkinElmer) and an indicated content of ATP in 50 mM Tris-Cl (pH 6.8 ...
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bioRxiv - Cancer Biology 2019Quote: ... and 2 (Oxidizing Reagent; Perkin Elmer) for 1 min ...
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bioRxiv - Microbiology 2019Quote: ... 2 mM [32P]-PPi (Perkin Elmer), 90 µM alanine ...
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bioRxiv - Biochemistry 2021Quote: ... myo-[2-3H]inositol (NET114A001MC, PerkinElmer), UDP-[6-3H]GlcNAc (ART 1136 ...
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bioRxiv - Cancer Biology 2020Quote: ... Both supernatant and lysate were moved to separate counting vials and mixed with 3 mL scintillation fluid (Ultima Gold #6013321, Perkin Elmer). 14C levels were measured in each sample using a Packard Liquid Scintillation Analyzer ...
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bioRxiv - Microbiology 2021Quote: ... and the infectivity was determined by measuring the relative luciferase units (RLU) in the cell lysates using the Britelite plus system (PerkinElmer, USA). Viral infectivity was considered positive when the RLU value was at least 5-fold higher than the background RLU value in the parental NP-2 cell line ...
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bioRxiv - Cell Biology 2021Quote: ... The AlphaLISA® SureFire® Ultra™ assay was run in total volume 20 μL (using only 10 μL cell culture lysate sample) in 384 well plate (PerkinElmer, #6007290) following standard assay two step no-wash protocol ...
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bioRxiv - Physiology 2022Quote: ... Radioactivity was measured on 150 or 250 μl of muscle lysate by liquid scintillation counting (Ultima Gold and Tri-Carb 4910 TR, Perkin Elmer) and related to the specific activity of the incubation buffer.
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bioRxiv - Immunology 2022Quote: ... 150 μl of lysate was transferred to white solid 96-well plates for the detection of luminescence using a microplate luminometer (PerkinElmer, Ensight). The positive well was determined as ten-fold relative luminescence unit (RLU ...
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bioRxiv - Microbiology 2020Quote: ... Cells were incubated for 16–18 hours at 37°C before Fluc activity in cell lysates was detected using a plate luminometer (EnSpire 2300 Multilabel reader by Perkin Elmer) and the Bright-Glo Luciferase Assays System (Promega) ...
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bioRxiv - Microbiology 2023Quote: Stool material was homogenized using TissueLyzer from Quigen and the lysate was prepared for extraction with the Chemagic Stool gDNA extraction kit (Perkin Elmer) and extracted on the Chemagic 360 Instrument (Perkin Elmer ...
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bioRxiv - Genetics 2023Quote: ... was added in unison to the lysates and luminescence activity in counts per second were immediately recorded on a Victor X4 plate reader (Perkin Elmer). Using a multichannel pipet ...
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bioRxiv - Cell Biology 2024Quote: ... Cholesterol efflux was determined as % counts per minute (CPM) in the supernatant versus cell lysates with a liquid scintillation analyzer (Tricarb-2810TR, Perkin Elmer).
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bioRxiv - Cancer Biology 2024Quote: ... From each well was transferred 10 µL of the lysates to a 384-well Optiplate™ (Perkin Elmer, catalog no. 6007290). To each well was added Acceptor Mix (5 µL ...
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bioRxiv - Microbiology 2024Quote: ... and the uptake of 14C(U) l-arginine was measured in the bacterial lysates using a liquid scintillation counter (Perkin Elmer).
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bioRxiv - Neuroscience 2021Quote: Autoradiographic procedure for the analysis of 3H-2-deoxyglucose (2-DG; 1.5μCi/g body weight, #NET549A001MC, PerkinElmer) localization was adapted from the method by Sokoloff et al ...
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bioRxiv - Biochemistry 2020Quote: ... pH 7.4) with 10 μM 2-deoxy-Glucose and 0.5 μCi/mL 3H-2-deoxy-glucose (Perkin Elmer). Cytocholasin B (5 μM ...
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bioRxiv - Systems Biology 2021Quote: ... The His6-MBP-PDZ concentrations of soluble cell lysate fractions were evaluated with a microfluidic capillary electrophoretic system (Caliper LabChip GXII, PerkinElmer, Waltham, Massachusetts) and were adjusted to 4 μM by dilutions ...
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bioRxiv - Biochemistry 2020Quote: ... 20 μL of lysate was added in triplicate to a 384-well white-walled plate (ProxiPlate-384 Plus, shallow-well microplate, PerkinElmer, Waltham, MA) and read on an EnVision plate reader (PerkinElmer ...
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bioRxiv - Microbiology 2022Quote: Coupled in vitro transcription and translation reactions were carried out using the Promega TnT® Coupled Reticulate Lysate System labelled with radioactive [35S] methionine (PerkinElmer Inc.) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Half of the cell Triton X lysate from each well was pipetted into a scintillation vial and 4 ml of scintillation fluid (#6013329, Ultima GoldTM, Perkin Elmer, USA) was added ...
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bioRxiv - Biochemistry 2023Quote: ... The other procedures were the same as the Zn transport assay except that the cell lysates were mixed with the Ultima Gold cocktail (PerkinElmer, Cat# L8286) and 55Fe associated with the cells was quantified using a liquid scintillation counter (LS 6500 Multi-Purpose Scintillation Counter).
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bioRxiv - Cell Biology 2020Quote: ... λ-32P-ATP (2 μCi; Perkin Elmer) was included in reactions to enable measurement of phosphorylation during assay optimization using a phosphorimager (Typhoon FLA7000 ...