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Citations for PerkinElmer :
51 - 100 of 237 citations for Anti PI3 kinase p150 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... 9-1-1 having an N-terminal 7-residue kinase recognition sequence on Mec3 was radiolabeled to a specific activity of approximately 70 cpm/fmol with [γ-32P] ATP (PerkinElmer Life Sciences, Inc) using the recombinant catalytic subunit of cAMP-dependent protein kinase produced in E ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary anti-HRP conjugate antibodies were used and immunoreactivity was visualized using Western LightingTM (PerkinElmer). Immunoreactivity was developed on film or using a ChemiDoc™ imaging system (Biorad) ...
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bioRxiv - Genomics 2019Quote: ... Anti-rabbit or anti-mouse HRP conjugated antibodies were used to detect protein by chemiluminescence using ECL (Perkin Elmer, Western Lightning Plus ECL). Images were visualized by GeneSnap image analysis software (SynGene ...
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bioRxiv - Cancer Biology 2022Quote: ... Addition of a Europium-labelled antibody specific to the phosphopeptide (LANCE Ultra Europium-anti-phospho-Crosstide (anti-GSK-3α Ser21, Perkin Elmer, TRF0202-M) allows proximity-dependent energy transfer from the Europium donor to the Ultra UlightTM acceptor ...
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bioRxiv - Molecular Biology 2022Quote: ... Double fluorescence TSA was carried out using FITC-labeled doRPS12 and DIG-labeled doSRSF1 RNA probes detected with HRP-labeled anti-fluorescein and Cy3-conjugated Tyramide or HRP-labeled anti-digoxigenin antibodies with Cy5-conjugated Tyramide (PerkinElmer, Waltham, MA, USA). After probe hybridization and washing ...
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bioRxiv - Developmental Biology 2022Quote: ... Then those sections were stained with anti-MYC antibody with TSA plus fluorescein system (Perkin Elmer) followed by antigen retrieval to remove antibodies ...
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bioRxiv - Physiology 2024Quote: ... Fluorescein was detected with a horseradish peroxidase-conjugated anti-fluorescein antibody (RRID: AB_2737388; PerkinElmer, Waltham, MA) and visualized by using the TSA Plus Fluorescein System (PerkinElmer) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... animals were incubated overnight with peroxidase-conjugated antibodies at 4°C (anti-DIG-POD, Roche 1:500 dilution and anti-DNP, Perkin Elmer, 1:200 dilution) followed by the amplification of the signal with fluorophore-conjugated tyramides (1:100 TSA reagent diluents ...
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bioRxiv - Developmental Biology 2022Quote: ... anti-mouse Chd7 primary antibody was followed by signal amplification with goat anti-rabbit IgG horseradish peroxidase (1: 500) (Perkin Elmer Inc: NEF812001EA, Waltham, MA). The tyramide signal amplification plus Cy3 Kit (1:1500 ...
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bioRxiv - Developmental Biology 2020Quote: ... Signals from anti-OAZ and Lmx1a antibodies were amplified using the Tyramide Signal Amplification Kit (Perkin Elmer), according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Fluorescein was detected with an anti-fluorescein antibody conjugated to horseradish peroxidase (RRID: AB_2737388; PerkinElmer, Waltham, MA) and visualized using the TSA Plus Fluorescein System (PerkinElmer) ...
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bioRxiv - Physiology 2024Quote: ... fluorescein was detected with an anti-fluorescein antibody conjugated to horseradish peroxidase (RRID: AB_2737388; PerkinElmer, Waltham, MA) and visualized using the TSA Plus Fluorescein System (PerkinElmer) ...
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bioRxiv - Cell Biology 2021Quote: ... Reactions were started by adding 40 µl IR in alkyl maltoside micelles to 10 µl radioactive kinase reaction buffer (0.4 mM ATP, 1 µCi [γ-32P]-ATP (PerkinElmer, 10 mCi/mL, 25-50 cpm/mol), 1 mM MgCl2 ...
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bioRxiv - Physiology 2020Quote: ... Blots were then washed with TBST prior to incubation with HRP-linked anti-mouse secondary antibody (Perkin Elmer) for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... Following TBST washes the membranes were incubated in the relevant secondary antibody: Goat anti-rabbit IgG (Perkin Elmer/ NEF812001EA), diluted (1:10,000 ...
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bioRxiv - Developmental Biology 2020Quote: ... incubated 1h in blocking buffer and then incubated overnight with an anti-DNP-POD antibody (1:1000, Perkin Elmer). The next day embryos were washed in PBS-Tween ...
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bioRxiv - Cell Biology 2023Quote: ... 10 nM full-length 6x-His-tagged human G3BP1 produced in mammalian cells was added to the reaction mix containing test compound and 0.5 nM anti-His-Tb antibody (PerkinElmer). Assays were initiated by adding 4 nM FAM-labeled PEG6-USP10 24-mer peptide probe (FAM-PEG6-GALHSPQYIFGDFSPDEFNQFFVT ...
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bioRxiv - Developmental Biology 2023Quote: ... was used to detect the anti-pH3 antibody after detection of ISH probe by using TSA plus (Perkin Elmer) green (FP1168).
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies were detected by incubation with peroxidase-conjugated anti-mouse and anti-rabbit antibodies (1:10000, Jackson Immuno Research Laboratories) followed by reaction with ECL-Plus reagent (Western Lighting, PerkinElmer) and exposure to autoradiography films ...
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bioRxiv - Neuroscience 2021Quote: ... and Lumi4-Tb cryptate conjugated anti-IP1 antibody (emission at 620 nm) was detected using an Envision plate reader (PerkinElmer). Results were calculated from the 665/620 nm ratio and normalised to the maximum response stimulated by acetylcholine.
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bioRxiv - Neuroscience 2022Quote: ... membranes were washed and incubated with the goat anti-rabbit IgG HRP-labeled secondary antibody (PerkinElmer, Waltham, MA, USA; NEF812001EA) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... anti-mouse or anti-rabbit secondary antibody (1:10000; Jackson Immuno Research Laboratories) followed by treatment with ECL-plus reagent (Western Lighting, PerkinElmer) and exposure to autoradiography films ...
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bioRxiv - Developmental Biology 2021Quote: ... Antisense RNA probes labeled with DNP were used in all in situ hybridizations and probe detection was performed using a HRP-coupled anti-DNP antibody (PerkinElmer) and a fluorescein coupled tyramide (Vize et al. ...
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bioRxiv - Microbiology 2022Quote: ... The membranes were then incubated with anti-IgG rabbit antibodies conjugated to horseradish peroxidase (PerkinElmer Life Sciences (Boston, MA, USA), and developed using the Western Lightning Chemiluminescence Reagent Plus (PerkinElmer) ...
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bioRxiv - Neuroscience 2022Quote: ... then incubated for 30 min at 25 °C with shaking at 400 RPM with 9 ng/well of DELFIA Eu-N1 anti-mouse antibody in DELFIA assay buffer (PerkinElmer), washed with 3 × 100 μl of PBST before enhancing with 80 μl of DELFIA Enhancement Solution (PerkinElmer) ...
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bioRxiv - Biochemistry 2022Quote: ... 5 μL time points were taken and quenched with 1.33 mM EDTA then brought to 20 μL final volume for detection using 1 nM LANCE Ultra Europium anti-H3K4me1/2 antibody (TRF0402, PerkinElmer) and 50 nM LANCE Ultra Ulight-Streptavidin (TRF0102 ...
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bioRxiv - Immunology 2021Quote: ... Cells were immunostained with DAPI and Alexa488-conjugated anti-N antibody (CR3009; produced in-house) and imaged using an Opera Phenix (Perkin Elmer). The ratio of infected (Alexa488+ ...
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bioRxiv - Physiology 2019Quote: ... Cells were incubated with an anti-ERα antibody (F-10) overnight followed by Cy3 Tyramide signal amplification (PerkinElmer-TSA-Plus-Cy3) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: AlphaScreen-based peptide recruitments assays were performed using streptavidin coated donor beads and anti-His-tag antibody coated AlphaLisa acceptor beads (Perkin Elmer). The assays were very sensitive to the distance of the N-terminus to the LXXLL NR-box motif and the use of a flexible Ahx linker ...
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bioRxiv - Genomics 2021Quote: ... the anti-DIG-HRP antibody was detected with the TSA-Cy3 substrate (1:50, TSATM PLUS Cy3 kit, NEL 745001KT, Perkin Elmer) for 10 min at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was probed with a goat anti-mouse antibody conjugated to horseradish peroxidase and bands were visualized using the Western Lighting Plus-ECL reagent (Perkin Elmer). When performing the KatG expression analysis in response to C10 treatment ...
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bioRxiv - Cell Biology 2023Quote: ... or ECL anti-rabbit antibodies (NA934V; 17434242) (Amersham) were used for detection of probed proteins using enhanced chemiluminescence (ECL)(Perkin Elmer). Chemiluminescence was detected using an iBright FL1500 (ThermoFisherScientific ...
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bioRxiv - Neuroscience 2019Quote: ... The sections were incubated overnight at 4ºC with horseradish peroxidase-conjugated anti-fluorescein antibody (diluted 1:1000 and 1:500 for detection in PMm/PMg and other nuclei, respectively; PerkinElmer, Waltham, MA) and Dylight 549-conjugated goat anti-rabbit IgG (diluted 1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated with a horseradish peroxidase-conjugated anti-fluorescein antibody (diluted 1:500 with DIG-1, PerkinElmer, Foster City, CA) for 1 h and washed twice with DIG-1 for 5 min each time ...
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bioRxiv - Biochemistry 2022Quote: ... Detection reagents were added stepwise with 30 min incubation of anti-H3K4me3 antibody and Ulight-Streptavidin with reaction time points followed by 1 hr incubation with Europium anti-rabbit antibody in 384 well white microplates (PerkinElmer OptiPlate-384). TR-FRET emission at 665 nm and at 615 nm by 320 nm excitation with 50 μs delay and 100 μs integration time was measured using a Molecular Devices SpectraMax M5e plate reader ...
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bioRxiv - Immunology 2024Quote: ... Five-micrometer serial sections were stained with a human-specific anti-COXIV antibody and scanned using the Vectra® Polaris Automated Imaging System (PerkinElmer, USA) at 20×magnification ...
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bioRxiv - Neuroscience 2023Quote: ... Reactions were initiated by adding 2.5 µM ATP at 27°C and were terminated after 60 min with 10 µL of 2.5X Stop Solution/Detection Mix containing 20mM EDTA and 4nM LANCE Ultra Europium-anti-phospho-PKC (Ala25Ser) peptide antibody (Perkin Elmer, Waltham, MA). After incubation at 27°C for another 60 min ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 5 μL of 1/100 dilution of the Alexa Fluor® 647-anti cAMP antibody solution in the Detection Buffer (PerkinElmer Life and Analytical Sciences) and 10 μL of 1/5000 dilution of Eu-W8044 labeled streptavidin and Biotin-cAMP in the Detection Buffer (PerkinElmer Life and Analytical Sciences ...
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bioRxiv - Biochemistry 2021Quote: ... 5 μL of 1/100 dilution of the Alexa Fluor® 647-anti cAMP antibody solution in the Detection Buffer (PerkinElmer Life and Analytical Sciences) and 10 μL of 1/5000 dilution of Eu-W8044 labeled streptavidin and Biotin-cAMP in the Detection Buffer (PerkinElmer Life and Analytical Sciences ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were detected using HRP-linked secondary antibodies together with Western Lightning Plus-ECL (Perkin Elmer). Fiji (ImageJ ...
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bioRxiv - Neuroscience 2020Quote: ... antibodies plus Cy3-TSA (1:200, PerkinElmer). Sections were cover-slipped using Prolong Diamond Antifade Mounting media with DAPI (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... Antibodies were detected via ECL reagents (PerkinElmer).
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bioRxiv - Cancer Biology 2024Quote: ... blocked in antibody (Ab) Diluent (Perkin Elmer), incubated for 30 min with the primary antibody ...
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bioRxiv - Developmental Biology 2019Quote: ... or a tyramine-amplified fluorescent antibody (Perkin Elmer).
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bioRxiv - Microbiology 2020Quote: A matrix with all antibodies provided by PerkinElmer was tested for the ability to detect NP.
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bioRxiv - Neuroscience 2024Quote: ... and blocked in antibody diluent (PerkinElmer, Waltham, MA). Sections were then sequentially stained for Iba-1 (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... All antibodies were diluted in TNB solution from PerkinElmer. Sections were then incubated for 30 min in streptavidin-horseradish peroxidase that was diluted (1:200 ...
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bioRxiv - Developmental Biology 2020Quote: ... The TSA-Kit secondary antibody system (Perkin Elmer, NEL763E001KT) was utilized with Lef1 immunostaining.
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bioRxiv - Cancer Biology 2024Quote: ... 10 min with horseradish peroxidase-conjugated secondary polymer (anti-mouse/anti-rabbit, Perkin Elmer), and 10 min with horseradish peroxidase-reactive OPAL fluorescent reagents (Perkin Elmer) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Bound antibodies were detected using horseradish peroxidase-conjugated secondary antibodies and visualized by chemiluminescence using Western Lightning Plus-ECL reagent (Perkin Elmer, United States). Band intensities were analysed using ImageJ (National Institutes of Health ...