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Citations for PerkinElmer :
51 - 100 of 440 citations for Alpha 2 HS glycoprotein Fetuin A Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... and Caliper LabChip® GX DNA HS (Caliper, A PerkinElmer Company, Hopkinton, MA, USA) assays ...
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bioRxiv - Biophysics 2023Quote: ... Filter plates were dried at 96℃ before scintillation sheet (MeltiLex B/HS, Perkin Elmer) was melted on to the filter ...
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bioRxiv - Microbiology 2020Quote: ... Bacterial growth (OD600) was determined approximately 20 h later utilizing a Enspire Alpha multimode plate reader (PerkinElmer). To calculate the MIC90 ...
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bioRxiv - Biophysics 2021Quote: ... HEK293 TauRD-P301S-CFP/YFP expressing biosensor cells were plated in poly-L-lysine coated 96-well plates (PerkinElmer) at a density of 20000 cells/well and subsequently transfected with pre-incubated mixtures of tau seeds/CAP1 ...
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bioRxiv - Biochemistry 2023Quote: ... 25 µg/mL acceptor (nickel chelate) and donor (anti-his europium; both Perkin Elmer). All components were diluted to working concentrations in alphaLISA buffer (50 mM HEPES ...
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bioRxiv - Biophysics 2023Quote: ... LeuT was mixed with Yttrium Silicate Copper (YSi-Cu) His-tag SPA beads (PerkinElmer) and [3H]leucine in binding buffer (200 mM NaCl ...
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bioRxiv - Bioengineering 2022Quote: ... The emitted signal intensity was measured using standard Alpha settings on an EnSpire® Multimode Plate Reader (PerkinElmer). Measurements are expressed as technical duplicates (mean+/- SD ...
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bioRxiv - Cancer Biology 2023Quote: ... an Alpha SureFire Ultra Multiplex Phospho-EGFR (Tyr1068) and Total EGFR Assay Kit (PerkinElmer MPSU-PTEGFR-K-HV) was used ...
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bioRxiv - Neuroscience 2019Quote: ... were transfected into the indicated cells (HEK293/17: 4×105/well; CHO: 2.5×104/well, BMVECs: 5×103/well) in 96-well plates (PerkinElmer, 6005680) in triplicate ...
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bioRxiv - Biochemistry 2021Quote: ... microsomes prepared from HEK293 cells expressing RyR2 were incubated for 1 h at 25 °C with 5 nM [3H]ryanodine (PerkinElmer) in reaction media containing 0.17 M NaCl ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Binding experiments were performed on membranes prepared from wild-type and HA-MOP-transfected HEK293 cells using [3H]DAMGO (51.7 Ci/mmol) (PerkinElmer NET902250UC) as described previously52 ...
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bioRxiv - Genomics 2019Quote: ... The DNA fragments and libraries were characterized using a Labchip GX Touch HS Kit (Perkin Elmer) or TapeStation (Agilent ...
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bioRxiv - Microbiology 2021Quote: ... Viral particles were titrated using an HIV-1 p24Gag Alpha-Lisa kit and an Envision plate reader (Perkin Elmer) and/or by determining their infection titers on target cells.
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bioRxiv - Genomics 2021Quote: ... Insulin was quantified using the Insulin (human) AlphaLISA Detection Kit and the EnSpire Alpha Plate Reader (both Perkin Elmer) based on 1:10 and 1:200 dilutions for supernatant and insulin content ...
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bioRxiv - Microbiology 2022Quote: ... Viral particles were titrated using an HIV-1 p24Gag Alpha-Lisa kit and an Envision plate reader (Perkin Elmer). VSV-G pseudotyped HIV-1 (NL4.3 ...
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bioRxiv - Molecular Biology 2024Quote: ... from synthetic oligonucleotides (0.2 µM) and trace-labeled with 32P alpha-ATP (800 Ci/mmol, 12.5 µM, Perkin Elmer) in a 20 µl reaction according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Two days after transfection the Hek293 cells were washed with PBS and seeded into a 96 well black-white iso plate (Perkin Elmer) in pbs containing 5 mM glucose and supplemented with 5 µM of Nanoglo (Promega #N1120 ...
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bioRxiv - Neuroscience 2020Quote: ... Extracellular culture media were collected in order to dose secreted Aβ using Alpha-LISA assays (AlphaLISA Amyloid β1-X Kit, AL288C, PerkinElmer) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... to a fixed concentration of target (e.g., biotinylated PTM-defined nucleosome) with the appropriate Alpha Donor and Acceptor beads (Perkin Elmer) (Jain et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... was done using the same procedure for the cold transcription except for the use of 0.5 µl alpha-32P-labeled UTP (3000 Ci/mmol-10 mCi/ml) (Perkin Elmer) and 0.8 mM UTP (instead of 2 mM) ...
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bioRxiv - Immunology 2024Quote: ... with fluorescence detected on intensity (485 nm excitation and 530 nm emission) measured by an EnSpire Alpha Multimode Plate Reader (PerkinElmer).
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bioRxiv - Cancer Biology 2019Quote: ... with dsDNA HS (High Sensitivity) Assay Kit and qualified using LabChip (LabChip® GX Touch™ PerkinElmer).
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bioRxiv - Physiology 2020Quote: On the fifth day of EOS or PBS treatment 3H-2-deoxy-D-glucose (2DG; 10uCi/mouse; PerkinElmer, Waltham, MA) was given to 4 hour fasted individually housed mice ...
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bioRxiv - Molecular Biology 2021Quote: ... Plates were read at 450 nm and readings were processed to observe binding patterns of tested molecules (Perkin Elmer, Enspire Alpha). For every experiment ...
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bioRxiv - Neuroscience 2019Quote: ... Phosphorylation was determined by alpha-screen bead-based technology using the Amplified Luminiscent Proximity Homogeneous Assay kit (PerkinElmer, Waltham, MA, USA) and the Enspire Multimode Plate Reader (PerkinElmer ...
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bioRxiv - Molecular Biology 2023Quote: ... Reactions were initiated by the addition of GTP to a final concentration of 40 uM containing a trace amount of [alpha-32P]-GTP (Perkin Elmer) and incubated for 20 min at 30°C ...
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bioRxiv - Physiology 2020Quote: On the fifth day of EOS or PBS treatment 3H-2-deoxy-D-glucose (2DG; 10 uCi/mouse; PerkinElmer, Waltham, MA) was given to 4-hour fasted individually housed mice ...
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bioRxiv - Neuroscience 2020Quote: ... Double immunofluorescence was performed using tetramethyl rhodamine labelled secondary antibody for alpha-synuclein and NSF with the fluorescein signal amplification kit (Perkin Elmer, UK). Following adequate washes to remove background fluorescence ...
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bioRxiv - Cancer Biology 2019Quote: ... AlphaLISA assays were performed in 96-well half area white plates (6005560) and read in an EnSpire Alpha 2300 Mutilabel Plate reader (Perkin Elmer, Inc.). A 96-well white plate was filled with 5 µl of EVs or 10 µl of CM ...
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bioRxiv - Neuroscience 2023Quote: ... cAMP levels were measured with an Amplified Luminescent Proximity Homogeneous Assay (ALPHA) Screen cAMP assay kit as instructed by the manufacturer (PerkinElmer, Waltham, MA).
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bioRxiv - Immunology 2021Quote: ... with dsDNA HS (High Sensitivity) Assay Kit and LabChip GX Touch using a High Sensitivity DNA chip (Perkin Elmer). Libraries were then equimolarly pooled and quantified by qPCR using the KAPA library quantification kit (Roche) ...
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bioRxiv - Cell Biology 2023Quote: ... with dsDNA HS (High Sensitivity) Assay Kit and LabChip GX Touch using a High Sensitivity DNA chip (Perkin Elmer). Libraries were then equimolarly pooled and quantified by qPCR using the KAPA library quantification kit (Roche) ...
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bioRxiv - Biophysics 2019Quote: ... pH 7.5/0-800 mM NaCl (equimolar substitution of NaCl with Tris/Mes)/20% glycerol/0.1 mM TCEP/0.1% DDM using 1.25 mg/mL copper His-tag PVT SPA beads (Perkin Elmer). Total binding was corrected for the non-proximity-based signal to determine the specific binding activity of each LeuT variant and data were normalized to the maximal binding observed for LeuT-WT that was assayed in parallel in all individual measurements ...
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bioRxiv - Cell Biology 2023Quote: ... 10 nM full-length 6x-His-tagged human G3BP1 produced in mammalian cells was added to the reaction mix containing test compound and 0.5 nM anti-His-Tb antibody (PerkinElmer). Assays were initiated by adding 4 nM FAM-labeled PEG6-USP10 24-mer peptide probe (FAM-PEG6-GALHSPQYIFGDFSPDEFNQFFVT ...
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Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: ... Activity of the SMG1i against other PI3 kinase family members (PI3Kα, PI3Kβ, PI3Kγ, PI3Kδ, MTOR) was evaluated using Amplified Luminescent Proximity Homogeneous Assay (Alpha) Screen assays (Perkin Elmer and Echelon Biosciences). PI3K or mTOR protein (produced in Sf9 cells ...
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bioRxiv - Immunology 2022Quote: ... The quality and average fragment size of the final libraries were determined using DNA 1K/12K/Hi Sensitivity Assay LabChip and DNA High Sensitivity Reagent Kit (PerkinElmer). Libraries with 16 unique dual indexes were pooled and sequenced on a NextSeq 500 benchtop sequencer (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... The average fragment size of the final libraries was determined using DNA 1K/12K/Hi Sensitivity Assay LabChip (760517, PerkinElmer)) and DNA High Sensitivity Reagent Kit (CLS760672 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Animals were injected intravenously 24h after the last pharmacological treatment with Hypoxisense 680 (2 nmol/100 uL per mouse, Perkin Elmer, Life Sciences, Zaventem, Belgium), and the Hypoxisense 680 was measured in vivo 24h post-injection using the IVIS Spectrum (Perkin Elmer ...
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bioRxiv - Biophysics 2019Quote: AlphaScreen-based peptide recruitments assays were performed using streptavidin coated donor beads and anti-His-tag antibody coated AlphaLisa acceptor beads (Perkin Elmer). The assays were very sensitive to the distance of the N-terminus to the LXXLL NR-box motif and the use of a flexible Ahx linker ...
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bioRxiv - Biochemistry 2020Quote: ... peptides were tested for their ability to disrupt a second Alphascreen assay in which donor and acceptor beads were reacted with 50 nM concentrations of a peptide consisting of a biotinylation site and a His-tag peptide (PerkinElmer Life Sciences).
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bioRxiv - Cancer Biology 2020Quote: ... 2’-14C]-cystine (PerkinElmer) for 5 minutes at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ERK1/2 phosphorylation was detected with the AlphaScreen SureFire ERK1/2 assay kit (PerkinElmer). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... was added to control wells before addition of 2-[3H]deoxyglucose (2-DG) (PerkinElmer). During the final 5 min 2-DG (0.25 μCi ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 mM DTT and 2 μCi/mL S-adenosyl-L-[methyl-14C] methionine (PerkinElmer). After 3h incubation ...
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bioRxiv - Bioengineering 2021Quote: ... A Wizard 2 (Perkin Elmer) gamma counter was used to quantify tracer uptake in organs ...
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bioRxiv - Physiology 2019Quote: ... [3H]2-DG (Perkin Elmer) was injected retro-orbitally in a bolus of saline containing 66.7 μCi/mL [3H]2DG corresponding to ~9-10 μCi/mouse (6 μL/g body weight ...
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bioRxiv - Cell Biology 2021Quote: ... 2’-14C]-Cystine (PerkinElmer, NEC854010UC) and washed three times in ice-cold PBS containing cold cystine (1 mM) ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μCi of 2-Deoxy-D-[1-2-3H] glucose (PerkinElmer, Boston, MA, USA # NET328A001MC) and 10μM non-radioactive 2-DG (Sigma Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mL of each component was added to 2 mL Ultima Gold scintillation fluid (PerkinElmer), and radioactivity counted on the MicroBeta2 scintillation counter (PerkinElmer) ...
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bioRxiv - Immunology 2020Quote: ... 2 × 106 treated cells were incubated with 2 MBq of Na2 CrO4 (#NEZ030001MC, PerkinElmer, Waltham, MA) for 2 hours at 37 °C in a 5% CO2 atmosphere and were used as target cells ...