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Citations for PerkinElmer :
51 - 100 of 1530 citations for 3 Hydrazino 5 methyl 4H 1 2 4 triazol 4 ylamine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... and counted for 2 min after a 4 hr delay on a Perkin Elmer Betaplate 1205 liquid scintillation counter (Perkin Elmer). Nonspecific binding was determined with 1.0 µM dihydroergotamine.
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bioRxiv - Neuroscience 2023Quote: ... The choroid plexus was subsequently swiftly rinsed in cold isotope-free HEPES-aCSF containing 2 mM ouabain (4°C) and transferred to a scintillation vial and dissolved in 100 μl Solvable (6NE9100, Perkin Elmer). For the light and dark phase influx experiment ...
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bioRxiv - Microbiology 2022Quote: ... were performed in a HORIBA JOBIN YVON Spectrofluorometer (FluoroMax – 4) (HORIBA Scientific, Edison, New Jersey, USA) in a fluorescence cuvette of path length 5 mm (Perkin Elmer B0631123), after putting 0.5ml of the sample solution in it ...
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bioRxiv - Microbiology 2020Quote: ... we used THP-1 cells (4×105 cells/ml) adhered in an optical 96-well plate (PerkinElmer) with 50 ng/ml phorbol 12-myristate 13-acetate (PMA ...
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bioRxiv - Biophysics 2020Quote: ... 100 nM hNatB was mixed with a saturating concentration of “MDVF” peptide substrate (500 μM) and varying concentrations (1.95 μM to 1 mM) of acetyl-CoA (14C-labeled, 4 mCi mmol−1; PerkinElmer Life Sciences) for 10-minute reactions ...
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bioRxiv - Molecular Biology 2023Quote: ... Transcription was initiated with addition of 12 pmol rrnBP1 PCR promoter fragment pre-mixed with 1 mM ATP and 4 μCi α-[32P]-CTP (3,000 Ci mmol−1; Perkin Elmer) at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... TSA Cyanine 3 or Cyanine 5 amplification kits (Perkin Elmer) were used according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... TSA fluorophores for cyanine 3 and cyanine 5 (Perkin Elmer) were used at a concentration of 1:1000 ...
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bioRxiv - Biochemistry 2022Quote: ... and mixed with 4 ml Ultima Gold scintillation cocktail (PerkinElmer). Each sample was counted for 5 min on a Beckman LS 6000SC liquid scintillation counter.
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bioRxiv - Pathology 2021Quote: ... the Opal 4-color fluorescent IHC kit was used (PerkinElmer). All images were captured and assessed for statistical analyzes using BZ-X analyzer (Keyence ...
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bioRxiv - Biochemistry 2023Quote: ... by incubation with 4 pmoles of [γ-32P]ATP (PerkinElmer) by polynucleotide kinase (NEB ...
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bioRxiv - Plant Biology 2023Quote: ... 4 ml of HionicFluor scintillating mixture (PerkinElmer, Waltham, MA, USA) was added to the samples ...
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bioRxiv - Biochemistry 2024Quote: ... substituting cold SAM with 4 μM 3H SAM (Perkin Elmer) 10 μL of the reaction were spotted on nitrate cellulose membranes and washed with 3 mL of 20 mM HEPES (pH 7.4 ...
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bioRxiv - Neuroscience 2021Quote: ... the free-floating sections were incubated overnight at 4°C with Streptavidin-Texas Red (1:400, NEL721001EA PerkinElmer). Finally ...
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bioRxiv - Molecular Biology 2023Quote: ... were measured using a radiometric assay that detects transfer of a [3H] methyl from the radio-labeled cofactor S-[Methyl-3H]-adenosyl-L-methionine (SAM, PerkinElmer, cat. NET155001MC) to a biotinylated peptide Akt1-K64 (QCQLMKTERPRPNTFIIRCLQW ...
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bioRxiv - Biochemistry 2022Quote: ... S-[methyl-3H] (3H-SAM) (PerkinElmer, 18.0 Ci/mmol); and 0.1 μM purified DNMT3A2 in assay buffer (50 mM sodium phosphate ...
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bioRxiv - Cell Biology 2022Quote: ... for 5 min in a VICTOR 3 microplate reader (Perkin Elmer). The results were expressed as % of the pool of controls.
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bioRxiv - Developmental Biology 2020Quote: ... Cyanine 3 (Cy3) or Cyanine 5 (Cy5) Fluorophore Tyramide by PerkinElmer was used ...
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bioRxiv - Microbiology 2024Quote: ... and 4 z positions (0 µm, 0.5 µm, 1 µm, and 1.5 µm) with an Opera Phenix microscope (Perkin Elmer). Colocalization analysis of LC3 ...
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bioRxiv - Cancer Biology 2021Quote: ... The PerkinElmer Opal 4-Color Manual IHC Kit (PerkinElmer/Akoya, NEL810001KT) was used for the tyramide signal amplification according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... we used the Opal 4-Color Fluorescent IHC Kit (PerkinElmer, USA) for immunofluorescence microscopy light ...
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bioRxiv - Biochemistry 2022Quote: ... then 5 mL Optiphase ‘HISAFE’ 2 scintillation cocktail (PerkinElmer) were added ...
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bioRxiv - Neuroscience 2020Quote: ... 3 and 6 hr later cells were labeled for 5 min with 1 mCi/ml 35S Protein Labeling Mix (PerkinElmer). Lysates from 25-ml culture triplicate samples were analyzed by SDS-PAGE and autoradiography.
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 0.76 µM labelled [methyl-3H] -AdoMet (Perkin Elmer) for 3 hours at 25°C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Alphascreen Surefire Akt1/2/3 (p-Ser473) Phosphorylation kit (PerkinElmer, TGRA4S), Collagenase Type II (Scimar Australia ...
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bioRxiv - Cell Biology 2021Quote: ... 20 μCi/ml myo-[2-3-H(N)] inositol (PerkinElmer, MA) and indicated treatment conditions ...
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bioRxiv - Neuroscience 2020Quote: ... TR-FRET readout was performed after 1 h incubation at 4°C using an EnVision 2105 Multimode Plate Reader (PerkinElmer) with previously defined measurement parameters53 ...
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bioRxiv - Neuroscience 2022Quote: ... 022-105721-00321-0001, POLATOM, as a marker for K+ transport, and 4 μCi ml-1 3H-mannitol as an extracellular marker, PerkinElmer) for 2min (influx ...
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bioRxiv - Molecular Biology 2021Quote: HDL-sRNA libraries were generated using the NEXTFlex Small RNA-Seq Kit v3 with 1:4 adapter dilution and 22 PCR cycles (PerkinElmer). Following PCR amplification ...
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bioRxiv - Molecular Biology 2024Quote: ... membranes were blocked with 5% BSA in Tris / HCl-buffered saline / 0.05% Tween (TBST) for 24 h at 4 °C and afterwards membranes were incubated for 1 h with HRP-Streptavidin (PerkinElmer; #NEL750001EA ...
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bioRxiv - Molecular Biology 2022Quote: ... was next added and incubated with the mixture at 4 °C for 1 hour before the measurements performed on Envision Readers (PerkinElmer). The fluorescently-labeled m6A-containing mRNA was used to adjust the gain factor ...
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bioRxiv - Microbiology 2022Quote: ... [4-14C] Isopentenyl diphosphate triammonimum salt (55.0 mCi/mml) was from PerkinElmer Life Sciences ...
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bioRxiv - Microbiology 2023Quote: ... added to 4 mL scintillation fluid (Ultima Gold™ XR, Perkin Elmer) and measured in a scintillation counter (Tri-Carb 4910 TR ...
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bioRxiv - Biochemistry 2023Quote: ... Incubations were done at room temperature (24°C) for 4h and the end-point fluorescence reading obtained with a multimode microplate reader (Envision or EnSight, Perkin Elmer) equipped with excitation 405 nm and emission 535 nm filters ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were imaged at 1 frame/30 s for 4 h at 37°C with 5% CO2 using a spinning disk confocal microscope (UltraVIEW VoX, PerkinElmer with a 63X 1.4 N.A. oil objective), followed by addition of 50 μg/ml PI (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... of incubation in an isotope solution containing rubidium (86Rb+) (1 μCi/ml, 022-105721-00321-0001, POLATOM) and 3H-mannitol (4 μCi/ml, NET101, Perkin Elmer). 86Rb+ acts as a K+ congener ...
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bioRxiv - Neuroscience 2023Quote: ... Thawed serum samples were diluted with 1xPBS 1:4 and assayed in triplicates using a fluorometer (PerkinElmer, VICTOR3V 1420 Multilabel counter) with excitation/emission of 485 nm / 535 nm (25 nm band-pass filter) ...
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bioRxiv - Cancer Biology 2023Quote: ... Following an HRP conjugation step a TSA Opal 570 1:150 (Tyramide Signal Amplification Opal 4-Color Manual IHC Kit, Perkin Elmer) was linked to the IRF7 antibody previously bound to the slide overnight ...
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bioRxiv - Genomics 2023Quote: ... only 100nM were radioactive ([Methyl-3H]-Choline Chloride; Perkin Elmer #NET109001MC), the rest was unlabeled (Choline Chloride ...
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bioRxiv - Microbiology 2020Quote: ... 2 μM ATP and 5 μCi [γ-32P] ATP (PerkinElmer) were used to start the reaction for 10 min ...
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bioRxiv - Genetics 2020Quote: ... and AlphaLISA unmodified Histone H3 Lysine 4 (H3K4) Cellular Detection Kit (AL719, PerkinElmer) for normalization ...
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bioRxiv - Cell Biology 2021Quote: ... and L-glutamine (4 mM) with 50 μCi/mL 35S-methionine (PerkinElmer, NEG009L005MC) for 20 min ...
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bioRxiv - Molecular Biology 2022Quote: ... compound 4 and DC591053 was measured by a LANCE Ultra cAMP kit (PerkinElmer). Ligands were verified for their bioactivity in the beginning in hRXFP4-CHO ...
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bioRxiv - Biochemistry 2024Quote: ... 2x donor mixtures were prepared by mixing 4 nM streptavidin-Eu (Perkin Elmer) with or without 2 nM yeast nucleosomes containing 177 bp of Widom 601 DNA (31+145+1 ...
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bioRxiv - Biochemistry 2023Quote: [4-14C]-cholesterol (52 mCi/mmol) was purchased from Perkin Elmer (Whaltham, USA). [4-14C]-5,6α-epoxycholestan-3β-ol ...
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bioRxiv - Bioengineering 2023Quote: ... mice were anesthetized with 2.5% Isoflurane (RAS-4 Rodent Anesthesia system, Perkin Elmer) and 150 mg/kg body weight D-Luciferin-K-Salt (PJK GmbH ...
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bioRxiv - Molecular Biology 2024Quote: ... mice were anaesthetized using 2.5% isoflurane in Rodent Anaesthesia System RAS-4 (PerkinElmer) and the luminescence signal was detected using IVIS spectrum in vivo imaging system (PerkinElmer ...
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bioRxiv - Biophysics 2021Quote: ... 50 nM SpNatC (WT or mutants) was mixed with 50 μM Ac-CoA (14C-labeled, 4 mCi mmol-1; PerkinElmer Life Sciences) and varying peptide concentrations (ranging from 1.95 μM to 500 μM ...
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bioRxiv - Molecular Biology 2023Quote: ... 3μM S-adenosyl-L-[methyl-3H] methionine (specific activity 18Ci/mmol, PerkinElmer). For unmodified DNA duplex ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.55μM S-adenosyl-L-[methyl-3H] methionine (specific activity 18Ci/mmol, PerkinElmer), 1.9μM AdoMet in 50mM Tris–HCl ...