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Citations for PerkinElmer :
51 - 100 of 1057 citations for 1 tert Butoxy 4 methyl 2 nitrobenzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... and counted for 2 min after a 4 hr delay on a Perkin Elmer Betaplate 1205 liquid scintillation counter (Perkin Elmer). Nonspecific binding was determined with 1.0 µM dihydroergotamine.
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bioRxiv - Neuroscience 2023Quote: ... The choroid plexus was subsequently swiftly rinsed in cold isotope-free HEPES-aCSF containing 2 mM ouabain (4°C) and transferred to a scintillation vial and dissolved in 100 μl Solvable (6NE9100, Perkin Elmer). For the light and dark phase influx experiment ...
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bioRxiv - Biochemistry 2021Quote: ... or 0.1 μM [1-14C]linoleic acid (2 μCi/dish, Perkin Elmer) bound to albumin (1:1 molar ratio ...
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bioRxiv - Molecular Biology 2023Quote: DNA methylation of replicating sperm or erythrocyte nuclei in egg extract was assayed by the incorporation of 3H-SAM (S-[methyl-3H]-adenosyl-L-methionine; Perkin Elmer, NET155H). Demembranated sperm nuclei were prepared as published previously (86) ...
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bioRxiv - Cancer Biology 2023Quote: ... 1×10^4 LN-229 cells were plated in CellCarrier-96 Ultra Microplates (PerkinElmer, #6055302) and incubated overnight in reduced serum media at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... 1 μM UTP) and 4 μCi of [α-32P]UTP (3000 Ci/mmol; Perkin Elmer), allowed to proceed for 1 h at room temperature ...
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bioRxiv - Biophysics 2020Quote: ... 100 nM hNatB was mixed with a saturating concentration of “MDVF” peptide substrate (500 μM) and varying concentrations (1.95 μM to 1 mM) of acetyl-CoA (14C-labeled, 4 mCi mmol−1; PerkinElmer Life Sciences) for 10-minute reactions ...
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bioRxiv - Molecular Biology 2023Quote: ... Transcription was initiated with addition of 12 pmol rrnBP1 PCR promoter fragment pre-mixed with 1 mM ATP and 4 μCi α-[32P]-CTP (3,000 Ci mmol−1; Perkin Elmer) at 37 °C ...
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bioRxiv - Biochemistry 2024Quote: ... [1-3H] RuBP was synthetized enzymatically from D-[2-3H] glucose (PerkinElmer, MA) following published protocols47 ...
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bioRxiv - Developmental Biology 2020Quote: Recombinant GST-SRF protein or synthetic SRF peptides were incubated for 3 h at 30 °C with GST-SET7 in the presence of 100 nCi of S-adenosyl-[methyl-14C]-L-methionine [14C-SAM] (Perkin Elmer, Waltham, MA, USA) in HMTase assay buffer (50 mM Tris–HCl [pH 8.5] ...
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bioRxiv - Physiology 2020Quote: ... TSA® Plus fluorophore for channel 2 (cyanine 3, PerkinElmer; 1:1000; 30 min), HRP blocker (15 min) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... which contains inhibitors and 1 μCi D-[2-3H]-glucose (23.4 Ci/mmol, PerkinElmer). After 30 s’ reaction ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 μL 10X T4 PNK buffer and 2 μL [γ-32P] ATP (Perkin Elmer). The volume was adjusted to 10 μL with water and incubated at 37°C for 1 hour ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: The level of phosphorylated extracellular signal-regulated protein kinase 1/2 (pERK1/2) was detected using the AlphaScreenTM SureFire Kit (PerkinElmer Life and Analytical Sciences). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... we used THP-1 cells (4×105 cells/ml) adhered in an optical 96-well plate (PerkinElmer) with 50 ng/ml phorbol 12-myristate 13-acetate (PMA ...
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bioRxiv - Physiology 2020Quote: ... i.p.) and a trace amount of [1-14C]2-deoxy-D-glucose (10 μCi; PerkinElmer). After 40 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Luciferase measurements were performed every 1 to 2 hours on an EnVision plate reader (PerkinElmer). Results were analysed by employing python (v3.8.5) ...
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bioRxiv - Physiology 2023Quote: ... ip) and a trace amount of 2-deoxy-D-[1-14C] glucose (10 µCi; PerkinElmer). Forty minutes later ...
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bioRxiv - Neuroscience 2021Quote: ... the free-floating sections were incubated overnight at 4°C with Streptavidin-Texas Red (1:400, NEL721001EA PerkinElmer). Finally ...
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bioRxiv - Microbiology 2024Quote: ... and 4 z positions (0 µm, 0.5 µm, 1 µm, and 1.5 µm) with an Opera Phenix microscope (Perkin Elmer). Colocalization analysis of LC3 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... WIN55212-2 (PerkinElmer) (10 µM ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 4 μL/well of ATPLite (PerkinElmer) was added to assay plates and incubated for 15 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 4 μL/well of ATPLite (PerkinElmer) was dispensed ...
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bioRxiv - Cell Biology 2020Quote: ... 4 μl/well of ATPLite (PerkinElmer) was added to assay plates and incubated for 15 min at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... emission 535 nm) measured for 1 hour at 2 minute intervals on an EnVision Xcite 2104 (PerkinElmer). Enzymatic activity of each mutant was measured with (+ Palm-CoA ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM MnCl2) supplemented with 10 μM ATP and 2 μCi γ-32P-ATP (blu002z250uc, Perkin Elmer) in 15μL reactions ...
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bioRxiv - Immunology 2022Quote: ... Uptake was initiated by addition of 1 μCi [3H]2-DG (25-30 Ci/mmol, PerkinElmer, NET549) and 0.1 mM unlabeled 2-DG (Sigma ...
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bioRxiv - Developmental Biology 2023Quote: ... Anesthetized (2% isoflurane vaporized in 1 L/min O2) dams were imaged (IVIS Lumina III, Perkin Elmer) with their ventral side facing the charge-coupled device camera (15 cm field of view ...
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bioRxiv - Cell Biology 2023Quote: ... cells on the SPA plate were suspended in 1 mL of DDW with 2% glusulase (PerkinElmer, #NEE154001EA) and digested with shaking at room temperature for 3 hr ...
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bioRxiv - Neuroscience 2020Quote: ... TR-FRET readout was performed after 1 h incubation at 4°C using an EnVision 2105 Multimode Plate Reader (PerkinElmer) with previously defined measurement parameters53 ...
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bioRxiv - Neuroscience 2022Quote: ... 022-105721-00321-0001, POLATOM, as a marker for K+ transport, and 4 μCi ml-1 3H-mannitol as an extracellular marker, PerkinElmer) for 2min (influx ...
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bioRxiv - Molecular Biology 2021Quote: HDL-sRNA libraries were generated using the NEXTFlex Small RNA-Seq Kit v3 with 1:4 adapter dilution and 22 PCR cycles (PerkinElmer). Following PCR amplification ...
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bioRxiv - Molecular Biology 2024Quote: ... membranes were blocked with 5% BSA in Tris / HCl-buffered saline / 0.05% Tween (TBST) for 24 h at 4 °C and afterwards membranes were incubated for 1 h with HRP-Streptavidin (PerkinElmer; #NEL750001EA ...
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bioRxiv - Molecular Biology 2022Quote: ... was next added and incubated with the mixture at 4 °C for 1 hour before the measurements performed on Envision Readers (PerkinElmer). The fluorescently-labeled m6A-containing mRNA was used to adjust the gain factor ...
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bioRxiv - Bioengineering 2022Quote: ... mice were shaved then anesthetized with 1–2% isoflurane using a calibrated vaporizer inside an IVIS Spectrum (PerkinElmer). Non-invasive longitudinal monitoring of tumour progression and nanoparticle release was followed by scanning mice with the IVIS Spectrum-bioluminescent and fluorescent imaging system (PerkinElmer) ...
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bioRxiv - Cancer Biology 2020Quote: ... 2’-14C]-cystine (PerkinElmer) for 5 minutes at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... Fluorescently labeled RNA was obtained by replacing 1/4 volume of CTP with Cyanine 3-CTP (Cy3-CTP) or Cyanine 5-CTP (Cy5-CTP) (PerkinElmer NEL581001EA) in the in vitro transcription reaction.
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bioRxiv - Neuroscience 2023Quote: ... of incubation in an isotope solution containing rubidium (86Rb+) (1 μCi/ml, 022-105721-00321-0001, POLATOM) and 3H-mannitol (4 μCi/ml, NET101, Perkin Elmer). 86Rb+ acts as a K+ congener ...
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bioRxiv - Neuroscience 2023Quote: ... Thawed serum samples were diluted with 1xPBS 1:4 and assayed in triplicates using a fluorometer (PerkinElmer, VICTOR3V 1420 Multilabel counter) with excitation/emission of 485 nm / 535 nm (25 nm band-pass filter) ...
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bioRxiv - Cancer Biology 2023Quote: ... Following an HRP conjugation step a TSA Opal 570 1:150 (Tyramide Signal Amplification Opal 4-Color Manual IHC Kit, Perkin Elmer) was linked to the IRF7 antibody previously bound to the slide overnight ...
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bioRxiv - Biochemistry 2021Quote: ... 20 μL of protein mixture was added to each well of a 1/2 area 96-well OptiPlate (PerkinElmer). Compounds were then added from DMSO stock to protein mixture (0.4 μl/well ...
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bioRxiv - Biochemistry 2021Quote: ... 20 μL of protein mixture was added to each well of a 1/2 area 96-well OptiPlate (PerkinElmer). FKBP12F36V competitor or p300-BRD competitor was then added from DMSO stock to protein mixture (0.4 μl/well ...
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bioRxiv - Immunology 2023Quote: ... Samples were incubated in triplicate (106 per sample) with 1 µCi of 2-[1,2-3H]-deoxyglucose (20 Ci/mmol; PerkinElmer) in uptake buffer for 4 min at room temperature and immediately spun through a layer of bromo-dodecane into a layer of 20% perchloric acid/8% sucrose to stop the reaction and separate cells from unincorporated 2-[3H]-deoxyglucose ...
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bioRxiv - Microbiology 2023Quote: ... assays were carried out by incubating 500ng of methyltransferases Rv2067c or DOT1L with 1–2 μg of recombinant mammalian histones or MtHU and 80μCi 3H-SAM (PerkinElmer) in a buffer containing 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Cancer Biology 2024Quote: ... CO2 trapping solution was prepared as follow: radiolabeled [1-14C] PA (2 µCi/mL; Perkin Elmer, Cat: #NET043001MC, US) and D-[14C(U)] glucose (2 µCi/mL ...
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bioRxiv - Biophysics 2021Quote: ... 50 nM SpNatC (WT or mutants) was mixed with 50 μM Ac-CoA (14C-labeled, 4 mCi mmol-1; PerkinElmer Life Sciences) and varying peptide concentrations (ranging from 1.95 μM to 500 μM ...
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bioRxiv - Molecular Biology 2021Quote: ERK1/2 phosphorylation was detected with the AlphaScreen SureFire ERK1/2 assay kit (PerkinElmer). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... was added to control wells before addition of 2-[3H]deoxyglucose (2-DG) (PerkinElmer). During the final 5 min 2-DG (0.25 μCi ...
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bioRxiv - Cell Biology 2024Quote: ... a glucose uptake tracer (14 C-2-deoxyglucose (2-DG) (Perkin Elmer, cat # NEC495001MC) was administered as a single bolus at a dose of 13 μCi over 1 minute at 75 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... A Wizard 2 (Perkin Elmer) gamma counter was used to quantify tracer uptake in organs ...