Labshake search
Citations for PerkinElmer :
901 - 950 of 1137 citations for 7 CHLORO 1 METHYL 1H PYRAZOLO 4 3 B PYRIDINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2021Quote: ... A 50 μl reaction mixture contains 1.6 mM [14C]-G3P (specific activity at 129.3 mCi mmol-1, PerkinElmer, USA), 0.8 mM CDP-DAG (Avanti ...
-
bioRxiv - Cell Biology 2020Quote: ... image stacks (5 × 1 μm optical sections) were acquired on a spinning disc confocal system (Ultraview Vox, Perkin Elmer) with a 60× 1.4 NA oil-immersion objective (Nikon ...
-
bioRxiv - Developmental Biology 2021Quote: ... Perkin-Elmer) coupled with fluorogenic substrate Alexa Fluor 594 tyramide for 30 s (1:200, TSA kit; Perkin Elmer). F-actin was stained with rhodamine-conjugated phalloidin (1:200 ...
-
bioRxiv - Microbiology 2020Quote: ... programmed with 1 µg DNA/50 µL and labeled with 20 µCi of L-[35S]-methionine (EasyTag, Perkin Elmer). Reactions were incubated at 30 °C for 30 min before adding L-methionine (1 mM final ...
-
bioRxiv - Cell Biology 2019Quote: ... p-ERK detection was performed using the AlphaLISA SureFire Ultra p-ERK 1/2 (Thr202/Tyr204) Assay Kit (PerkinElmer) per the manufacturer’s specifications in 1/2-area white 96-well plates ...
-
bioRxiv - Developmental Biology 2019Quote: ... slides were washed in TNT and treated with Fluorescein Amplification Reagent (Perkin Elmer, 1:50 in Plus Amplification Diluent) for 15 min at room temperature ...
-
bioRxiv - Microbiology 2020Quote: 2.5 × 105 THP-1 cells were seeded on 24-well optical bottom tissue culture plates (Perkin Elmer, 1450-606), treated with 100nM phorbol 12-myrystate-13-acetate (PMA ...
-
bioRxiv - Immunology 2021Quote: ... of the abdominal region was measured (exposure=1 sec., F/stop= 1.2, FOV=E, binning=medium, emissions filter=open) using Living Image software (PerkinElmer). Survival of the mice was recorded until 20 days after infection.
-
bioRxiv - Cancer Biology 2022Quote: ... ATP was measured in supernatants according to the protocol described by the ATPlite 1 step Luminescence Assay System (PerkinElmer). Luminescence readings were obtained from a Bioteck Synergy HT plate reader ...
-
bioRxiv - Neuroscience 2021Quote: ... incubated with 3% of H2O2 for 30 minutes at RT and blocked 1 hour RT with NEN blocking buffer prepared according to the manufacturer’s protocol (FP1012 PerkinElmer). AntiDIG antibody conjugated to HRP (1:2000 ...
-
bioRxiv - Neuroscience 2021Quote: ... incubated with 3% of H2O2 for 30 minutes at RT and blocked 1 hour RT with NEN blocking buffer prepared according to the manufacturer’s protocol (FP1012 PerkinElmer). AntiDIG antibody conjugated to HRP (1:1500 ...
-
bioRxiv - Cancer Biology 2022Quote: ... were were dispensed per well 1 h prior to imaging at Cytation5 image cytometer or Opera Phenix (Perkin Elmer) confocal screening microscope.
-
bioRxiv - Neuroscience 2020Quote: ... slices were incubated in a goat anti-rabbit horse radish peroxidase-conjugated IgG (1:200, NEF812001EA, PerkinElmer, Waltham, MA) for 30 min ...
-
bioRxiv - Plant Biology 2020Quote: ... Two 100-μl fractions were collected in 50 μl of 1.5 M Tris-HCl (pH 8.0) and 1 ml of Ultima Gold LLT scintillation cocktail (Perkin Elmer). Radioactivity was measured using a liquid scintillation analyser (Packard).
-
bioRxiv - Biochemistry 2021Quote: ... 20 μL of protein mixture was added to each well of a 1/2 area 96-well OptiPlate (PerkinElmer). Compounds were then added from DMSO stock to protein mixture (0.4 μl/well ...
-
bioRxiv - Biochemistry 2021Quote: ... 20 μL of protein mixture was added to each well of a 1/2 area 96-well OptiPlate (PerkinElmer). FKBP12F36V competitor or p300-BRD competitor was then added from DMSO stock to protein mixture (0.4 μl/well ...
-
bioRxiv - Neuroscience 2021Quote: ... Slides were washed again and incubated with the appropriate Opal tertiary antibody (1:100 in amplification diluent, Perkin Elmer) for 60 min ...
-
bioRxiv - Biochemistry 2021Quote: ... Lysates were incubated for 1 hour at room temperature before FRET was detected using an Envision plate reader (PerkinElmer). Emission of Lumi4TM-Tb cryptate was detected at 620 nm and emission of d2-conjugated IP1 at 665 nm ...
-
bioRxiv - Bioengineering 2021Quote: ... background luminescence was measured for each rat (1 sec exposure, field of view D, PerkinElmer IVIS, Waltham, MA, USA). Both knees were then injected with either 50 μL NanoLuc™ Luciferase (NL ...
-
bioRxiv - Microbiology 2021Quote: ... Viral particles were titrated using an HIV-1 p24Gag Alpha-Lisa kit and an Envision plate reader (Perkin Elmer) and/or by determining their infection titers on target cells.
-
bioRxiv - Microbiology 2021Quote: ... was used (at 1:20.000 dilution) for protein visualization in the presence of Western Lightning Plus-ECL enhanced chemiluminescence substrate (PerkinElmer). The WesternSure® Pre-stained Chemiluminescent Protein Ladder (Li-cor ...
-
bioRxiv - Physiology 2022Quote: ... EP3R immunoreactivity was visualized by reaction with FITC-conjugated tyramide (1:50; SAT701001KT; Tyramide Signal Amplification FITC Systems, PerkinElmer) for 3 min ...
-
bioRxiv - Microbiology 2022Quote: ... Viral particles were titrated using an HIV-1 p24Gag Alpha-Lisa kit and an Envision plate reader (Perkin Elmer). VSV-G pseudotyped HIV-1 (NL4.3 ...
-
bioRxiv - Neuroscience 2022Quote: ... was diluted at 1 µg/mL in PBS and passively absorbed on multiwell plates (SpectraPlate-384 HB, Perkin Elmer) overnight at 4 °C ...
-
bioRxiv - Microbiology 2024Quote: The lipid composition of bacterial strains was determined after labeling with [1-14C] acetate (60 mCi/mmol; Perkin Elmer), [32P] phosphate (1 Ci/mmol ...
-
bioRxiv - Molecular Biology 2023Quote: ... Slides were then washed three times with TNT buffer (TN buffer + 0.05% Tween-20) and incubated with FITC-tyramide diluted 1:100 in Amplification reagent (PerkinElmer) for 10 minutes at room temperature ...
-
bioRxiv - Microbiology 2023Quote: ... assays were carried out by incubating 500ng of methyltransferases Rv2067c or DOT1L with 1–2 μg of recombinant mammalian histones or MtHU and 80μCi 3H-SAM (PerkinElmer) in a buffer containing 50 mM Tris-HCl (pH 8.0) ...
-
bioRxiv - Microbiology 2023Quote: ... normalized by cell OD600 (final OD=0.4) and diluted 1: 100 into 150 μl of fresh TSB media in flat-bottomed 96-well plates (PerkinElmer). The cultures were overlaid with 100 μl of mineral oil (Sigma ...
-
bioRxiv - Cell Biology 2023Quote: ... for 1 h at 37 °C and imaged with the Opera Phenix High Content Screening System (#HH14000000, Perkin Elmer). Number of counted nuclei was determined with the image analysis software Harmony (PerkinElmer ...
-
bioRxiv - Biochemistry 2023Quote: ... 100 nM of WT FCE or mutant K286M was incubated with 1 μM of 32P-α-GTP (Perkin Elmer) in a 20 μL reaction containing 1x RNA capping buffer at room temperature for 1 minute ...
-
bioRxiv - Cancer Biology 2023Quote: ... 4T-1-Luc3 tumors and metastases were monitored by bioluminescence imaging on an IVIS Lumina III System (Perkin Elmer) twice a week after tumor implantation ...
-
bioRxiv - Neuroscience 2023Quote: ... pH 7.5/1 μM fluoxetine) containing 50 pM of the DAT ligand (125I) RTI55 (PerkinElmer Life Sciences, Boston, USA) for 60 min ...
-
bioRxiv - Bioengineering 2023Quote: We used hairless SKH-1 mice for live animal imaging on IVIS Spectrum In vivo Imaging System (Perkin Elmer). For ovalbumin quantification ...
-
bioRxiv - Immunology 2023Quote: ... Samples were incubated in triplicate (106 per sample) with 1 µCi of 2-[1,2-3H]-deoxyglucose (20 Ci/mmol; PerkinElmer) in uptake buffer for 4 min at room temperature and immediately spun through a layer of bromo-dodecane into a layer of 20% perchloric acid/8% sucrose to stop the reaction and separate cells from unincorporated 2-[3H]-deoxyglucose ...
-
bioRxiv - Microbiology 2024Quote: ... was added to the media at a 1:10,000 dilution and images were acquired using the Opera Phenix High-Content Screening System (PerkinElmer). Analysis was performed using Harmony 191 High-Content Imaging and Analysis Software (PerkinElmer ...
-
bioRxiv - Cancer Biology 2021Quote: In vitro methyltransferase assays were performed using 30 ul reaction buffer (50 mM Tris-HCl pH 7.8, 50 mM KCl, 5 mM MgCl2) with 1 ul 3H-AdoMet (Perkin Elmer, specifific activity = 55-85 Ci/mMole,0.55 μCi/ul ...
-
bioRxiv - Cell Biology 2020Quote: ... and labeled for 5 min with with 1 μL per OD of cells of EXPRESS 35S Protein Labeling Mix (PerkinElmer) for 5 or 10 min ...
-
bioRxiv - Molecular Biology 2020Quote: ... the reactions were started by adding 5× NTP mixture (1 mM for ATP, CTP and GTP, 0.1 mM for UTP) containing 2 µCi of [α−32P] UTP (PerkinElmer). Transcription reactions were performed at 37°C for 20 min ...
-
bioRxiv - Neuroscience 2021Quote: ... Molecular weight bands were visualized by incubation with peroxidase-conjugated anti-mouse or anti-rabbit secondary antibody (1:5000; Jackson Immuno Research Laboratories) followed by treatment with the ECL-plus reagent (Western Lighting; PerkinElmer) and exposure on G:Box Chemi XRQ system (Syngene) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Small RNA libraries were prepared from 1 ng of RNA using the NEXTflex Small RNA-seq Kit v3 (Perkin Elmer). The size distribution of the libraries was assessed on a Bioanalyzer with a DNA High Sensitivity kit (Agilent Technologies) ...
-
bioRxiv - Molecular Biology 2021Quote: ... The radiolabeled probes (200 bp) were generated by PCR using (γ-32P) dCTP 0.1 mM (3000 Ci. mmol-1 PerkinElmer). The primes used were ...
-
bioRxiv - Neuroscience 2019Quote: ... Primary antibodies were detected by incubation with peroxidase-conjugated anti-mouse and anti-rabbit antibodies (1:10000, Jackson Immuno Research Laboratories) followed by reaction with ECL-Plus reagent (Western Lighting, PerkinElmer) and exposure to autoradiography films ...
-
bioRxiv - Biochemistry 2019Quote: RAW264.7 cells in 24-well plates were labeled by overnight incubation with DMEM containing 1% fetal bovine serum and 0.5 μCi/mL [3H]cholesterol (Perkin Elmer). The next day ...
-
bioRxiv - Cancer Biology 2019Quote: ... 10 nmol of chemerin-9 in 25 μL iodination buffer (0.5 M sodium phosphate, pH 7.4) were mixed with 1 mCi carrier-free Na125I (NEZ033L010MC, PerkinElmer, Waltham, USA) in an HPLC glass vial with microvolume insert ...
-
bioRxiv - Biochemistry 2021Quote: ... The filter paper was air dried for 1 hour and then placed into 2 ml of scintillation liquid (Ultima Gold, PerkinElmer), and radioactivity was counted using a scintillation counter (Tri-Carb B2810TR ...
-
bioRxiv - Bioengineering 2020Quote: ... Azide functionalization was confirmed with Fourier-transform infrared spectroscopy (FTIR, Supplemental Figure 1, F Perkin Elmer Frontier MIR/NIR spectrometer) and quantified with electrospray ionization-mass spectrometry (ESI-MS ...
-
bioRxiv - Neuroscience 2021Quote: ... 1×104 HEK-293T cells were seeded in culture medium without antibiotics in white 96-well culture plates (Perkin Elmer) prior to transfection ...
-
bioRxiv - Cell Biology 2021Quote: ... 10mM MgCl2 and 1 mM MnCl2) supplemented with 10 μM ATP and 2 μCi γ-32P-ATP (blu002z250uc; Perkin Elmer) in 15μl reactions ...
-
bioRxiv - Cancer Biology 2021Quote: ... HRP-conjugated secondary anti-rabbit and anti-mouse (1:5000) were used and detected by using the enhance Chemiluminescent Substrate (PerkinElmer). The bands were visualized using the ChemiDoc Imaging System (BioRad).
-
bioRxiv - Molecular Biology 2022Quote: Undifferentiated H1-OCT4-GFP-INS:tdT-luciferase hPSC and the differentiated β-like cells were incubated with 15mg/mL D-luciferin for 1 min before imaging with an IVIS Spectrum system (PerkinElmer), with an exposure of 1 min and mid-bin setting ...