Labshake search
Citations for PerkinElmer :
851 - 900 of 928 citations for Somatostatin Receptor 1 SSTR1 Rabbit Polyclonal affinity purified Alexa488 labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... Thawed serum samples were diluted with 1xPBS 1:4 and assayed in triplicates using a fluorometer (PerkinElmer, VICTOR3V 1420 Multilabel counter) with excitation/emission of 485 nm / 535 nm (25 nm band-pass filter) ...
-
bioRxiv - Cancer Biology 2023Quote: ... #7074) for 1 hr at room temperature and visualized using Konica Minolta Film Processor with Western Lightning Plus (Perkin Elmer, NEL104001) or Immobilon Crescendo Western HRP substrate (Millipore ...
-
bioRxiv - Cancer Biology 2023Quote: ... Following an HRP conjugation step a TSA Opal 570 1:150 (Tyramide Signal Amplification Opal 4-Color Manual IHC Kit, Perkin Elmer) was linked to the IRF7 antibody previously bound to the slide overnight ...
-
bioRxiv - Genomics 2023Quote: ... and streptavidin-conjugated Atto-425 (Bio Trend) at a concentration of 1:400 using TSA-biotin (TSA Plus Biotin Kit, Perkin Elmer). All nuclei were DAPI-stained ...
-
bioRxiv - Cancer Biology 2023Quote: ... HRP-coupled anti-mouse-HRP was used (1:2500, Amersham) and detection was done by chemiluminescence (Western Lightning Plus ECL, Perkin Elmer) using the Amersham Imager 600 (GE Healthcare).
-
bioRxiv - Systems Biology 2023Quote: ... 0.5 mg/ml was added 1-hour prior to medium collection and fluorescence intensities were analyzed with plate reader (PerkinElmer 2030).
-
bioRxiv - Biochemistry 2023Quote: ... The amount of glycosylated Rac1 was determined by liquid scintillation counting of each sample for 1 min using a Tri-carb 2910TR scintillation counter (Perkin Elmer). The initial rates of the reaction were fit to the Michaelis Menten equation (eq 1 ...
-
bioRxiv - Molecular Biology 2023Quote: ... were cultured at 6000 cells per well in DMEM (+10% FBS, +1% P/S) on Perkin Elmer LLC CellCarrier-96 Ultra Microplates (Perkin Elmer) coated with PLO overnight ...
-
bioRxiv - Immunology 2023Quote: ... was injected intraperitoneally per mouse 15 minutes prior to imaging for 1 minute exposure on In Vivo Imaging Spectrum Bioluminescent and Fluorescent Imaging Systems (IVIS) (Perkin Elmer) to confirm infection ...
-
bioRxiv - Cancer Biology 2023Quote: ... Immuno-complexes were incubated for 20 min at 30°C in 30 μl of kinase buffer with a final concentration of 50 μM ATP and [γ-32P] ATP (1 x 10−2 μCi/pmol; PerkinElmer). Reactions were stopped by adding 5X LB ...
-
bioRxiv - Genetics 2024Quote: ... was done for 1 hour at room temperature and following the addition of the western lightening ECL Pro ECL (Perkin Elmer), images were then directly recorded using Biorad Molecular Imager® Chemi DocTM XRS ...
-
bioRxiv - Molecular Biology 2024Quote: ... the remaining 20% of the beads (supplemented with 40 µl of 2X ROTI®Load and boiled at 95°C for 10 min) were subjected to SDS-PAGE for immunoblotting together with 1% input samples using HRP-streptavidin conjugate (PerkinElmer; #NEL750001EA) or anti-p65 (Santa Cruz Biotechnology ...
-
bioRxiv - Molecular Biology 2023Quote: ... Fluorescently labeled RNA was obtained by replacing 1/4 volume of CTP with Cyanine 3-CTP (Cy3-CTP) or Cyanine 5-CTP (Cy5-CTP) (PerkinElmer NEL581001EA) in the in vitro transcription reaction.
-
bioRxiv - Neuroscience 2024Quote: ... washed one time with PBS and one time with PBS+DAPI (1:10 000) and subsequently imaged on the Opera Phenix High Content Screening System (Perkin Elmer) on a 40X water objective ...
-
bioRxiv - Bioengineering 2023Quote: UV-vis spectra of the medium and hydrogel samples were measured using a plastic cuvette (1 cm light path) with a UV-visible light spectrophotometer (Lambda 35, Perkin Elmer). The transmittance was measured over the range of 800-400 nm.
-
bioRxiv - Cell Biology 2024Quote: ... After two washes of 5min in 1X RNAscope wash buffer slides were incubated with 1:1500 Cyanine 3 amplification reagent (PerkinElmer, #FP1170012UG), or 1:1000 Cyanine 5 amplification reagent (PerkinElmer ...
-
bioRxiv - Developmental Biology 2021Quote: ... M13mp18 ssDNA was added as a template for DNA replication at the indicated concentrations in presence of α-;32PdCTP (3000Ci mmol−1, Perkin Elmer). At the indicated time points half of the samples were neutralized in 10 mM EDTA ...
-
bioRxiv - Neuroscience 2019Quote: ... Confluent cultures were changed by quiescent medium for 24 h before they were labelled for 4 h with 1 μCi/ml [3H] arachidonic acid (PerkinElmer Life Sciences). Washing cells once with PBS containing 0.1% free fatty acid albumin and twice with PBS alone ...
-
bioRxiv - Cancer Biology 2021Quote: ... cells were fixed with 2% PFA at RT for 30 min and stained with the Hoechst 33342 dye (1 μg/ml) at 37 °C and 5% CO2 before imaging with the Operetta™ high content imaging system (PerkinElmer Inc.). Forty fields were acquired from each well using appropriate filter sets ...
-
bioRxiv - Developmental Biology 2022Quote: ... Tyramide signal amplification reaction was performed using Cy3 fluorescent dye (diluted 1:50 using amplification diluent) (Perkin Elmer Life Sciences, Inc., NEL753001kt). The embryos were co-stained with CrebA and Crb antibodies for visualizing SG nuclei and apical cell boundaries ...
-
bioRxiv - Immunology 2021Quote: ... Virus-mAb/plasma-containing media was then aspirated from cells and 100 μL of a 1:2 dilution of SteadyLite Plus (Perkin Elmer, 6066759) in PBS with Ca++ and Mg++ was added to cells ...
-
bioRxiv - Neuroscience 2021Quote: ... sections were incubated in channel 1-specific HRP (323104) for 15min and Cy3-TSAP (1.5K, tyramine signal amplification plus, NEL744E001KT; PerkinElmer Waltham, MA, USA) for 30 min followed by a 15 min incubation in HRP blocker (323107) ...
-
bioRxiv - Cell Biology 2021Quote: ... media was removed and replaced with serum-free Modified Eagles Medium (MEM; Mediatech) containing 1 µCi/ml of 3H-myo-inositol (PerkinElmer Life Sciences). Forty-eight hrs post-infection ...
-
bioRxiv - Biochemistry 2019Quote: ... the reaction mixtures in Flash plates were incubated for 1 hour and the CPM were measured using Topcount plate reader (Perkin Elmer, www.perkinelmer.com). The CPM counts in the absence of compound for each data set were defined as 100% activity ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: Lipodisqs labelled with Cy5 either bound to the polymer SMA or to the lipid DLPE (Supplementary Table 1) were used to study the biodistribution in healthy mice using an IVIS® Spectrum in vivo imaging system (Perkin Elmer). Mice were intravenously injected with these two formulations of Lipodisqs (injected volume of 100 μl with a dose of 100 mg/kg lipid ...
-
bioRxiv - Pathology 2021Quote: ... incubated for 1 hour and imaged for brightfield and Hoechst staining using the Operetta CLS™ High-Content Analysis System (PerkinElmer, Inc.). Stained nuclei were counted using the Harmony® software (PerkinElmer ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... 3-plex positive control probe or with a cocktail of Abcb5-C1 and Abcb4-C2 probes with a 1:750 dilution of TSA-Fluorescein Plus and TSA-Cyanine 3 Plus (PerkinElmer, Shelton, CT), respectively ...
-
bioRxiv - Immunology 2021Quote: ... Virus-mAb/plasma-containing media was then aspirated from cells and 100 μL of a 1:2 dilution of SteadyLite Plus (Perkin Elmer, 6066759) in PBS with Ca++ and Mg++ was added to cells ...
-
bioRxiv - Developmental Biology 2021Quote: ... the RNAscope Multiplex Fluorescent Reagent Kit (v.2) and TSA Plus reagents (Perkin Elmer; 1:500 dilution for Cy3 and Cy5 dyes) or Opal dyes (Akoya Biosciences ...
-
bioRxiv - Microbiology 2021Quote: ... Virus-mAb-containing media was then aspirated from cells and 50 μL of a 1:2 dilution of SteadyLite Plus (Perkin Elmer, 6066759) in PBS with Ca++ and Mg++ was added to cells ...
-
bioRxiv - Immunology 2021Quote: ... HIV-1 p24 content (produced by the backbone vector) was quantified using the Alliance p24 ELISA Kit (PerkinElmer Health Sciences, Cat# NEK050B001KT). Relative luminescence units (RLUs ...
-
bioRxiv - Bioengineering 2022Quote: ... permeabilized for 30 min in 0.5% Triton X-100 in PBS and stained overnight with 1 μmol/mL CNA35-OG488 [30] and 0.2 nmol/ml OsteoSenseTM 680 (NEV10020EX, PerkinElmer, Waltham, MA, USA) at 4 ºC to visualize collagen and hydroxyapatite ...
-
bioRxiv - Microbiology 2023Quote: Prokaryotic (bacteria and archaea) heterotrophic production was estimated using the 3H-leucine incorporation method (Perkin Elmer, specific activity 100 Ci mmol−1). Water samples (1.7 mL each ...
-
bioRxiv - Biochemistry 2023Quote: ... Reactions were initiated by adding an equal volume of assay buffer containing 1 μm [γ-32P]GTP (~50 cpm/fmol, Perkin Elmer) at 30 °C ...
-
bioRxiv - Genetics 2022Quote: ... The physical titer of vector preparations was measured using the HIV-1 Gag p24 antigen immunocapture assay kit (PerkinElmer, Waltham, MA, USA) and expressed as p24 ng/ml ...
-
bioRxiv - Biochemistry 2023Quote: ... BRB80 was mixed with a 1/10 volume of 10X nucleotide exchange buffer (10 mM GTP, 30 nM GTP α-32P (PerkinElmer BLU506H250UC), 1 mM nocodazole ...
-
bioRxiv - Cell Biology 2023Quote: ... 5 μl cAMP Eu-cryptate reagent and 5 μl of anti-cAMP-d2 working solution were added to the 384-well plates and incubated for 1 h.54 Fluorescence signals were detected at 620/665 nm using the Multimode Plate Reader (PerkinElmer EnVision 2105).55 Data were analyzed with GraphPad Prism 9.0 ...
-
bioRxiv - Molecular Biology 2023Quote: ... GLuc production was measured as a surrogate for IFN-β promoter activation by adding 25 µl coelenterazine-solution (1 µg/ml in H2O) was to 25 µl supernatant.reader (Perkin Elmer, Waltham, USA) and luminescence was measured ...
-
bioRxiv - Cancer Biology 2023Quote: ... The amount of eATP was analyzed according to the protocol described by the manufacturer of ATPlite 1-step Luminescence Assay System (PerkinElmer, Cat# 6016736) either at 0 hours (eATP concentrations were determined immediately ...
-
bioRxiv - Cancer Biology 2024Quote: ... 1:3 dilution steps) using a Janus Gripper with 384-channel Modular Dispense Technology dispensing head (revvity, previously Perkin Elmer, MA, USA). Afterwards ...
-
bioRxiv - Cell Biology 2022Quote: ... Luminescence was measured at 1 h post PathHunter© detection reagent addition using Victor3 plate reader (Perkin Elmer 0.5-s/well integration time). Data were normalized to control treated with the highest concentration of ligand (100%) ...
-
bioRxiv - Cancer Biology 2019Quote: ... Cells were stained with Hoechst (1/5000) for 30 min and fluorescence was monitored using victor x4 2030 Multilabel Reader (PerkinElmer, Waltham, MA, USA) with excitation wavelength at 350 nm ...
-
bioRxiv - Bioengineering 2020Quote: ... cells were resuspended in 100 μL of medium at a 4:2:1:1:1 ratio and transferred to ultralow attachment (ULA) round bottom 96-well plates (CellCarrier Spheroid ULA 96-well Microplates, PerkinElmer, Waltham, MA, USA). In another method ...
-
Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... All the videos (2 min, 4 frames s-1) were acquired at 37 °C using a Nikon TIRF microscopy system (Perkin Elmer, Waltham, MA) on an inverted Ti microscope equipped with a 100× objective and an ImageEM C9100-13 camera (Hamamatsu Photonics ...
-
bioRxiv - Cancer Biology 2022Quote: ... Organoid/hydrogel cultures were serum starved overnight before treatment with EGF (20 ng/mL) and promegestone/R5020 (1, 10, 100 nM; Perkin Elmer, Cat. #: NLP00400) and 10 nM R5020 with or without the addition of FAK (1 μM ...
-
bioRxiv - Immunology 2020Quote: ... The IgGs and ACE2 were incubated in the plates for 1 hour and ACE2-Fc-Biotin was detected by DELFIA Eu-N1 Streptavidin (Perkin Elmer, 1244-360) using the DELFIA-TRF system (Perkin Elmer).
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Oxaliplatin loaded Cedrus libani Sporopollenin (CD-SP-OX) were analyzed in the range of 4000-650 cm-1 using an FT-IR spectrometer (Perkin Elmer 100, USA) under ambient conditions.
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... lung tissues harvested from euthanized mice were soaked for 10 min in pre-warmed (37°C) PBS with 2% FBS and a 1:10 dilution of Xenolight RediJect Coelenterazine h (Cat# 706506, Perkin Elmer, Waltham, MA). Following a 12-15 min incubation ...
-
bioRxiv - Developmental Biology 2020Quote: ... we followed the protocol provided by the commercial company except that all samples were stained in 1 to 80 dilution of Cy3-conjugated or FLU-conjugated Tyrimide substrates for 60 minutes (Perkin Elmer NEL753001 Kit).
-
bioRxiv - Cell Biology 2020Quote: In vitro kinase reactions were developed in a final volume of 50 μl containing 0.02 mM [γ-32P]ATP (1 µCi per tube, Perkin Elmer, Massachusetts, USA), 50 mM Tris-HCl ...