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Citations for PerkinElmer :
851 - 900 of 1046 citations for 1 6 Chloropyridazin 3 yl piperidine 4 carboxamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... 100 nM of WT FCE or mutant K286M was incubated with 1 μM of 32P-α-GTP (Perkin Elmer) in a 20 μL reaction containing 1x RNA capping buffer at room temperature for 1 minute ...
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bioRxiv - Bioengineering 2023Quote: We used hairless SKH-1 mice for live animal imaging on IVIS Spectrum In vivo Imaging System (Perkin Elmer). For ovalbumin quantification ...
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bioRxiv - Molecular Biology 2023Quote: ... Slides were then washed three times with TNT buffer (TN buffer + 0.05% Tween-20) and incubated with FITC-tyramide diluted 1:100 in Amplification reagent (PerkinElmer) for 10 minutes at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... was added to the media at a 1:10,000 dilution and images were acquired using the Opera Phenix High-Content Screening System (PerkinElmer). Analysis was performed using Harmony 191 High-Content Imaging and Analysis Software (PerkinElmer ...
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bioRxiv - Physiology 2024Quote: ... and ion concentrations were tested using the Perkin Elmer Pinaacle 900T AAS Flame (Perkin Elmer, Massachusetts, USA; Table 1).
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bioRxiv - Cancer Biology 2024Quote: ... CO2 trapping solution was prepared as follow: radiolabeled [1-14C] PA (2 µCi/mL; Perkin Elmer, Cat: #NET043001MC, US) and D-[14C(U)] glucose (2 µCi/mL ...
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bioRxiv - Cancer Biology 2021Quote: In vitro methyltransferase assays were performed using 30 ul reaction buffer (50 mM Tris-HCl pH 7.8, 50 mM KCl, 5 mM MgCl2) with 1 ul 3H-AdoMet (Perkin Elmer, specifific activity = 55-85 Ci/mMole,0.55 μCi/ul ...
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bioRxiv - Cell Biology 2020Quote: ... and labeled for 5 min with with 1 μL per OD of cells of EXPRESS 35S Protein Labeling Mix (PerkinElmer) for 5 or 10 min ...
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bioRxiv - Molecular Biology 2020Quote: ... the reactions were started by adding 5× NTP mixture (1 mM for ATP, CTP and GTP, 0.1 mM for UTP) containing 2 µCi of [α−32P] UTP (PerkinElmer). Transcription reactions were performed at 37°C for 20 min ...
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bioRxiv - Neuroscience 2021Quote: ... Molecular weight bands were visualized by incubation with peroxidase-conjugated anti-mouse or anti-rabbit secondary antibody (1:5000; Jackson Immuno Research Laboratories) followed by treatment with the ECL-plus reagent (Western Lighting; PerkinElmer) and exposure on G:Box Chemi XRQ system (Syngene) ...
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bioRxiv - Molecular Biology 2021Quote: ... Small RNA libraries were prepared from 1 ng of RNA using the NEXTflex Small RNA-seq Kit v3 (Perkin Elmer). The size distribution of the libraries was assessed on a Bioanalyzer with a DNA High Sensitivity kit (Agilent Technologies) ...
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bioRxiv - Molecular Biology 2021Quote: ... The radiolabeled probes (200 bp) were generated by PCR using (γ-32P) dCTP 0.1 mM (3000 Ci. mmol-1 PerkinElmer). The primes used were ...
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bioRxiv - Biochemistry 2021Quote: ... The filter paper was air dried for 1 hour and then placed into 2 ml of scintillation liquid (Ultima Gold, PerkinElmer), and radioactivity was counted using a scintillation counter (Tri-Carb B2810TR ...
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bioRxiv - Bioengineering 2020Quote: ... Azide functionalization was confirmed with Fourier-transform infrared spectroscopy (FTIR, Supplemental Figure 1, F Perkin Elmer Frontier MIR/NIR spectrometer) and quantified with electrospray ionization-mass spectrometry (ESI-MS ...
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bioRxiv - Neuroscience 2021Quote: ... 1×104 HEK-293T cells were seeded in culture medium without antibiotics in white 96-well culture plates (Perkin Elmer) prior to transfection ...
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bioRxiv - Cell Biology 2021Quote: ... 10mM MgCl2 and 1 mM MnCl2) supplemented with 10 μM ATP and 2 μCi γ-32P-ATP (blu002z250uc; Perkin Elmer) in 15μl reactions ...
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bioRxiv - Cancer Biology 2021Quote: ... HRP-conjugated secondary anti-rabbit and anti-mouse (1:5000) were used and detected by using the enhance Chemiluminescent Substrate (PerkinElmer). The bands were visualized using the ChemiDoc Imaging System (BioRad).
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bioRxiv - Molecular Biology 2022Quote: Undifferentiated H1-OCT4-GFP-INS:tdT-luciferase hPSC and the differentiated β-like cells were incubated with 15mg/mL D-luciferin for 1 min before imaging with an IVIS Spectrum system (PerkinElmer), with an exposure of 1 min and mid-bin setting ...
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bioRxiv - Microbiology 2022Quote: ... Infection was quantified after 24 h by FACS (for HIV-1-mCherry) or by adding 50 μl/well britelite plus reagent (PerkinElmer) and measurement on a Pherastar FS (BMG ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were washed in PBSTw and then incubated in FITC-Tyramide (Perkin-Elmer) 1:100 in amplification reagent (Perkin Elmer), followed by several washes in PBSTw ...
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bioRxiv - Neuroscience 2020Quote: Larval stage 1 animals (L1) were imaged in a UltraView VoX spinning-disc confocal microscope (PerkinElmer Life and Analytical Sciences). Embryonic imaging was performed via dual-view inverted light sheet microscopy (diSPIM)64,65 ...
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bioRxiv - Neuroscience 2021Quote: ... during 2 hours at RT followed by a 10 minutes incubation at RT with TSA plus Biotin System (Biotin TSA 1:250, NEL749A001KT PerkinElmer). After several washes ...
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bioRxiv - Neuroscience 2021Quote: ... during 2 hours at RT followed by a 10 minutes incubation at RT with TSA Biotin System (Biotin TSA 1:100, NEL700A001KT PerkinElmer). After several washes ...
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bioRxiv - Biochemistry 2020Quote: ... was transferred to 384-well plates and diluted to 1 mM in DMSO using a JANUS Varispan Automated Workstation (PerkinElmer). The U2AF1S34F–U2AF2–SF1 protein and FlDEK variant oligonucleotide were plated at a total 20 μL reaction volume per well ...
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bioRxiv - Systems Biology 2020Quote: ... the DIG-labeled probe was visualized using a tyramide-Cy3 Plus kit (1:50 dilution, 15-minute incubation, Perkin Elmer). Following washes in phosphate buffered saline (PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... Plates were incubated at RT for 1 h and the fluorescence intensity measured at 620 nm and 650 nm by an EnVision multilabel plate reader (PerkinElmer).
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bioRxiv - Plant Biology 2020Quote: ... or without (for pPcUBI (I) and (II)) 1 μM Brassinazole medium were imaged using an Ultra View Vox Spinning disc confocal imaging system (PerkinElmer) mounted on an Eclipse Ti inverted microscope (Nikon ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and 20 μl of the reaction mixture was incubated at 26°C for 1 h in a 384-well AlphaPlate (PerkinElmer). Subsequently ...
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bioRxiv - Neuroscience 2020Quote: ... anti-mouse or anti-rabbit secondary antibody (1:10000; Jackson Immuno Research Laboratories) followed by treatment with ECL-plus reagent (Western Lighting, PerkinElmer) and exposure to autoradiography films ...
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bioRxiv - Microbiology 2021Quote: ... an equivalent of 500 ng of p24 Gag was lysed in buffer with 1% Triton (ELISA kit, Alliance Perkin Elmer) for 30 min on ice ...
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bioRxiv - Biochemistry 2021Quote: ... microsomes prepared from HEK293 cells expressing RyR2 were incubated for 1 h at 25 °C with 5 nM [3H]ryanodine (PerkinElmer) in reaction media containing 0.17 M NaCl ...
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bioRxiv - Neuroscience 2021Quote: ... Three-plex detection of IL-4Ra on glutaminergic and GABAergic neurons was achieved using Opal 520 (1:1000, Perkin Elmer), Opal 570 (1:1500 ...
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bioRxiv - Genomics 2020Quote: ... These steps were repeated starting from blocking for the second staining with primary antibody rat anti-CD8a 1:300, secondary antibody goat anti-rat (MP-7444, Vector) and opal 690 (fp1497, PerkinElmer) and the third staining with rat anti-CD45 1:1,000 ...
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bioRxiv - Microbiology 2020Quote: ... which were exposed to X-ray film for 1 h and detected by a storage phosphor system (Cyclone, PerkinElmer, USA).
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bioRxiv - Microbiology 2020Quote: Overnight bacterial cultures were diluted 1:200 in LB broth and 50μl bacterial suspension was added per well in CellCarrier Ultra 96 plates (Perkin Elmer) and left in a static incubator at 37°C for 2 hours ...
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bioRxiv - Microbiology 2022Quote: ... The plate was then incubated for 1 h at RT in the dark before the AlphaLISA signal was detected using an EnSpire Multimode Plate Reader (PerkinElmer). Negative control with binding buffer or only with one of the complexes were used to control the assay quality ...
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bioRxiv - Microbiology 2022Quote: ... 293T-ACE2 target cells were seeded at a density of 1 × 104 cells/well in 96-well luminometer- compatible tissue culture plates (PerkinElmer) 24 h before infection ...
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bioRxiv - Biochemistry 2022Quote: ... Plates were incubated at RT for 1 h and the fluorescence intensity measured at 620 nm and 665 nm by an EnVision multilabel plate reader (PerkinElmer).
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bioRxiv - Biochemistry 2022Quote: ... Steady-state binding signal was obtained after incubation of reactions for 1 hr at 25° C using a EnSight Multimode plate reader (PerkinElmer) with a 30 ms excitation at 680 nm and emission measured at 615 nm after 140 ms ...
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bioRxiv - Immunology 2022Quote: ... and primary human GM-CSF monocyte derived macrophages (MDM) were cultured in collagen-1-coated CellCarrier Ultra 96-well black walled plates (PerkinElmer) at a ratio of 1425 HSC:28500 MDM in FBM (Lonza ...
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bioRxiv - Microbiology 2022Quote: The lipid compositions of the bacterial strains were determined after labeling with [1-14C] acetate (specific activity: 55 mCi/mmol; PerkinElmer) or [35S] sulfate (specific activity ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were washed three times in 1 x PBS and imaged using the Opera Phenix high-content microscope (Perkin Elmer) at 63x magnification.
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bioRxiv - Plant Biology 2023Quote: ... Radioactivity was measured from the extract and the trichloroacetic acid precipitated protein pellet for 5 min with a scintillation counter (Wallac 1414 WinSpectral v.1.40; Wallac Oy, Turku, Finland) using 1 ml of Ultima Gold™ (PerkinElmer) liquid scintillation cocktail ...
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bioRxiv - Biochemistry 2022Quote: ... 5 μL time points were taken and quenched with 1.33 mM EDTA then brought to 20 μL final volume for detection using 1 nM LANCE Ultra Europium anti-H3K4me1/2 antibody (TRF0402, PerkinElmer) and 50 nM LANCE Ultra Ulight-Streptavidin (TRF0102 ...
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bioRxiv - Immunology 2022Quote: ... Virus–monoclonal antibody-containing medium was then aspirated from cells and 100Lµl of a 1:2 dilution of SteadyLite Plus (PerkinElmer, 6066759) in PBS with Ca2+ and Mg2+ was added to cells ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Plates were incubated in the dark for 1 hr at 37°C followed by measurement of fluorescence using an Envision plate reader (PerkinElmer) with standard AlphaScreenTM settings ...
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bioRxiv - Bioengineering 2022Quote: ... and Ethidium homodimer-1 (10μM) and cell death and viability data were measured using Operetta CLS High Content Analysis System (PerkinElmer, UK).
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bioRxiv - Cancer Biology 2024Quote: ... mice were used for orthotopic transplantation of 1×105 secondary tumor NES (tNES) cells and tumor growth was monitored using IVIS SpectrumCT In Vivo Imaging System (PerkinElmer) as previously described [14] ...
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bioRxiv - Cancer Biology 2023Quote: ... Each protein (5 µL) was mixed and incubated for 1 h in a 384-well AlphaPlate (PerkinElmer, Waltham, MA, USA). After diluting Alpha Screen Protein A Acceptor Beads (PerkinElmer ...
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bioRxiv - Cancer Biology 2024Quote: ... Image acquisition and quantification of POPO™-1 iodide staining intensity were done using the Harmony high-content imaging and analysis software 4.8 (PerkinElmer). Mesothelial clearance was performed by seeding MeT5A-GFP cells (15 ...