Labshake search
Citations for PerkinElmer :
801 - 850 of 1253 citations for Mouse Acrosomal Protein SP 10 ACRV1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: MCF10A cells transfected with control or ErbB3 siRNA were incubated with growth factor deprived DMEM media lacking methionine and cysteine supplemented with 10 μCi/ml 35S-methionine/cysteine (Perkin Elmer) for 30 minutes at 37°C ...
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bioRxiv - Microbiology 2021Quote: Vero E6 expressing TMPRSS2 or not were grown in DMEM supplemented with 10% FBS and seeded into white 96 well plates (PerkinElmer, 6005688) at a density of 20 thousand cells per well ...
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bioRxiv - Immunology 2021Quote: ... The plates were shaken at 300 rpm at room temperature for 10 minutes and relative light units (RLUs) were then read on an EnSight microplate reader (Perkin Elmer). Percent neutralization was determined by subtracting the mean background (uninfected cells with luciferase substrate alone ...
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bioRxiv - Immunology 2021Quote: ... incubated for 10 minutes at room temperature and the luciferase signal was quantified on a VICTOR Nivo plate reader (Perkin Elmer).
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bioRxiv - Cancer Biology 2022Quote: ... Image analysis was performed on the maximum projection image of 10 whole-well z-planes of the confocal imaging using Harmony software (Perkin Elmer).
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bioRxiv - Biochemistry 2022Quote: ... then centrifuged at 61 g for 10 seconds and the data were acquired in a Victor X 5 plate reader (Perkin Elmer) with an excitation wavelength of 480 nm and an emission wavelength of 535 nm ...
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bioRxiv - Molecular Biology 2022Quote: ... Then for the 6xHIS-tagged HAT module a 10 µL mixture of 5 µg/mL AlphaLISA Nickel chelate acceptors beads (PerkinElmer #AL108) and 10 µg/mL AlphaScreen donor beads (PerkinElmer #6760002) ...
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bioRxiv - Microbiology 2022Quote: 643nt pre-crRNA was in vitro transcribed with [a-32P]-ATP (10 mCi ml-1, 3000 Ci mmol-1, Perkin Elmer) as described above ...
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bioRxiv - Plant Biology 2023Quote: ... Stained SDS-PAGE bands were excised and transferred to scintillation vials and emulsified in 10 ml of Ultima Gold XR scintillation liquid (Perkin Elmer) in darkness at room temperature overnight ...
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bioRxiv - Microbiology 2022Quote: ... Samples were separated by SDS-PAGE using 10% or 15% (w/v) polyacrylamide gels and transferred to Protran nitrocellulose membranes (Perkin Elmer) using the Mini-Trans blot systems (BioRad ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The plates were incubated for 10 min on a shaker in the dark before measuring luminescence on the EnVision 2102 Multilabel reader (Perkin Elmer).
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bioRxiv - Immunology 2022Quote: Vero E6 cells were grown in DMEM supplemented with 10% FBS and seeded into clear bottom white 96 well plates (PerkinElmer, 6005688) at a density of 20,000 cells per well ...
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bioRxiv - Molecular Biology 2024Quote: ... and 50 ng was used for random priming reactions in the presence of the radiolabeled nucleotide [α-32P]-dCTP (3,000 Ci/mmol, 10 mCi/ml, Perkin Elmer) using a random primer DNA labeling kit (TaKaRa ...
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bioRxiv - Molecular Biology 2024Quote: ... the remaining 20% of the beads (supplemented with 40 µl of 2X ROTI®Load and boiled at 95°C for 10 min) were subjected to SDS-PAGE for immunoblotting together with 1% input samples using HRP-streptavidin conjugate (PerkinElmer; #NEL750001EA) or anti-p65 (Santa Cruz Biotechnology ...
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bioRxiv - Neuroscience 2024Quote: ... washed one time with PBS and one time with PBS+DAPI (1:10 000) and subsequently imaged on the Opera Phenix High Content Screening System (Perkin Elmer) on a 40X water objective ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 ng of heat-denatured DNA was incubated for 30 min at 37°C in a total volume of 25 μl containing random primer and 5 μl of [α-32P]-dCTP (3,000 Ci/mmol, 10 mCi/ml; Perkin Elmer). After the reaction ...
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bioRxiv - Cancer Biology 2024Quote: ... and incubated for 10 minutes at room temperature before images were taken using IVIS Spectrum In Vivo Imaging System (Perkin Elmer). Wnt-activation signal was calculated as the bioluminescent signal captured by the IVIS Spectrum normalized to the tumor volume recorded per animal ...
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bioRxiv - Cancer Biology 2024Quote: ... From each well was transferred 10 µL of the lysates to a 384-well Optiplate™ (Perkin Elmer, catalog no. 6007290). To each well was added Acceptor Mix (5 µL ...
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bioRxiv - Biophysics 2023Quote: Laser surgery was performed on a Zeiss Axio Imager M2m microscope equipped with a Yokogawa CSU-10 spinning disk confocal head (Perkin Elmer), a Hamamatsu EM-CCD camera and a Zeiss 40X ...
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bioRxiv - Cell Biology 2024Quote: ... for 30 minutes before changing to MOPS-buffered ‘Air medium’ with 10% serum but lacking luciferin and moving to an Opera Phenix Plus (Revvity Perkin Elmer, at the UCSC Chemical Screening Center RRID SCR_021114) ...
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bioRxiv - Cancer Biology 2023Quote: ... Immuno-complexes were incubated for 20 min at 30°C in 30 μl of kinase buffer with a final concentration of 50 μM ATP and [γ-32P] ATP (1 x 10−2 μCi/pmol; PerkinElmer). Reactions were stopped by adding 5X LB ...
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bioRxiv - Biochemistry 2024Quote: ... For competition binding experiments cell membranes expressing h5-HT3A WT or mutant receptors were incubated in 250 µL 10 mM HEPES buffer (pH 7.4) containing 0.3 nM [3H]-GR65630 (Perkin Elmer, Waltham, MA) and varying concentrations of VTX or analogues ...
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bioRxiv - Biochemistry 2023Quote: MLL1C binding assays (Figure 2C) were performed as above except using Nickel-chelate acceptor beads (10 μg/mL; Perkin Elmer AL108M), streptavidin donor beads (20 μg/mL ...
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bioRxiv - Molecular Biology 2023Quote: Glassware and plasticware used for RNA gel electrophoresis were soaked in 10% bleach for 5 minutes before washed by COUNT-OFF™ Surface Cleaner (PerkinElmer) and liberally rinsing in double-distilled water ...
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bioRxiv - Cancer Biology 2023Quote: ... We verified that these cells did not lose viability after overnight culture in serum-free medium relative to medium with 10% serum as determined by cell-based measurements of CBG bioluminescence in an IVIS Lumina (Perkin Elmer). We injected 1 × 105 breast cancer cells per mouse (n = 5 per each cell type) ...
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bioRxiv - Neuroscience 2023Quote: ... in the presence of 7 increasing concentrations (0.15 – 10 nM) of [3H]-raclopride (specific activity 82.8Ci/mmol, Perkin Elmer, #NET975250UC, Canada) for D2 binding ...
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bioRxiv - Immunology 2023Quote: ... Iodine-125 (125I) labeling was performed by incubating DTPA-bsAbs in iodogen coated Eppendorf tubes with Na125I (10−5 M NaOH, Perkin Elmer) in 0.5 M phosphate buffer (pH 7.4 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... were carefully added and plates were incubated for 10 min before luminescence reading in the EnVision 2103 Multilabel Reader (Perkin Elmer).
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bioRxiv - Cancer Biology 2023Quote: ... Dissociated cells were resuspended in 7.5 mg/mL BME-2 matrix gel at 2-5×104 cells/mL and seeded in 10 μL droplets to individual wells of 96-well Cell Carrier Ultra plates (Perkin Elmer). Once settled ...
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bioRxiv - Immunology 2023Quote: Vero E6 cells were grown in DMEM supplemented with 10% FBS and seeded into white-walled 96 well plates (PerkinElmer, 6005688) at a density of 20,000 cells per well ...
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bioRxiv - Molecular Biology 2023Quote: ... were cultured at 6000 cells per well in DMEM (+10% FBS, +1% P/S) on Perkin Elmer LLC CellCarrier-96 Ultra Microplates (Perkin Elmer) coated with PLO overnight ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were briefly vortexed and added to 20 mL liquid scintillation vials to which 10 mL of Ultima Gold was added (Perkin Elmer). The amount of glycosylated Rac1 was determined by liquid scintillation counting of each sample for 1 min using a Tri-carb 2910TR scintillation counter (Perkin Elmer) ...
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bioRxiv - Biochemistry 2023Quote: ... Reactions are incubated at 30 °C for 20 min prior to luminescence measurement for 10 s on the Victor X5 Multilabel Plate Reader (Perkin Elmer) by addition of 75 μl Renilla luciferase substrate (Promega) ...
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bioRxiv - Microbiology 2023Quote: ... The plate was incubated in the dark for 10-15 minutes before luminescence was read on a Victor3V 1420 Multilabel Counter (Perkin Elmer).
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bioRxiv - Biophysics 2024Quote: ... A six-point standard curve from 200 ppb to 1 ppb (200, 100, 50, 10, 5, 1) was prepared from elemental standards (Perkin Elmer), plus a 0-ppb blank ...
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bioRxiv - Cancer Biology 2024Quote: Luminescence imaging was carried out on days indicated using an IVIS luminescence imaging system 10 min after mice were injected with 10 µl/g of body weight 15 mg/mL in vivo grade D-Luciferin (PerkinElmer, 122799). Mice were sacrificed when reaching AAALAC-defined endpoints by CO2 asphyxiation followed by cervical dislocation ...
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bioRxiv - Microbiology 2024Quote: ... 10 ml cultures were grown in the presence of [32P]-phosphate (final concentration of 1 µCi ml-1; Perkin Elmer NEX060001MC) for until OD600 reached ∼1.5-2 ...
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bioRxiv - Cell Biology 2020Quote: ... The fourth was developed using TSA-biotin (TSA Plus Biotin Kit, Perkin Elmer) and streptavidin-conjugated Atto 425 (Sigma Aldrich).
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bioRxiv - Developmental Biology 2021Quote: ... and signals were detected using a Tyramide Signal Amplification (TSA) Plus kit (PerkinElmer). The embryos were imaged using a Zeiss Axio Imager A2 microscope or a Zeiss LSM 880 with an Airyscan confocal system.
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bioRxiv - Developmental Biology 2022Quote: ... and incubated in 1:200 TSA/amplification buffer (TSA Plus Fluorescence Kits; PerkinElmer) for 30 min to develop signals ...
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bioRxiv - Neuroscience 2021Quote: ... Signal was detected and amplified using the TSA staining Kit (Perkin Elmer (NEL0701001KT)) according to the manufacturer’s instructions
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bioRxiv - Molecular Biology 2021Quote: ... The chemiluminescence was raised with a Western Lightning Plus-ECL Kit (Perkin Elmer) according to the manufacturer’s protocol and detected with X-ray film (FUJI-FILM ...
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bioRxiv - Molecular Biology 2021Quote: GHRH-stimulated cAMP accumulation was measured by a LANCE Ultra cAMP kit (PerkinElmer). Briefly ...
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bioRxiv - Developmental Biology 2019Quote: ... Immunostaining was conducted using the TSA Plus Biotin Kit (Perkin Elmer, Waltham, MA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... The NEXTflex 16S V4 Amplicon-Seq Kit 2.0 (Bio Scientific, PerkinElmer, Waltham, Massachusetts) was used for the bacterial 16S library preparation according to the manufacturer’s instructions with the exception that 150 ng of material was used for amplification with 11 and 22 PCR cycles for the first and second rounds of amplifications ...
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bioRxiv - Biochemistry 2020Quote: After blocking using 0.5% Blocking Reagent (TSA Fluorescein System kit, PerkinElmer, Cat.No. NEL701A001KT) in Tris/HCl + NaCl ...
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bioRxiv - Plant Biology 2020Quote: ... The blots were visualized with the Western Lightning Plus Enhanced Chemiluminescence kit (PerkinElmer) and the X-Doc System (Bio-Rad) ...
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bioRxiv - Neuroscience 2020Quote: ... and Biotin-labeled probes were visualized by TSA kit (Perkin Elmer, MA, USA) including an anti-biotin streptavidin horse radish peroxidase-conjugate and fluorescein-tyramides as a substrate ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cAMP detection was using a Lance cAMP kit (PerkinElmer Life and Analytical Sciences), performed as previously described (Hager et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... Intracellular cAMP levels were tested by the LANCE Ultra cAMP kit (PerkinElmer, TRF0264) and the Multimode Plate Reader (PerkinElmer Envision ...