Labshake search
Citations for PerkinElmer :
801 - 850 of 912 citations for Human Complexin 2 CPLX2 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... 10 µg of total RNA was polyA-selected and processed using the NEXTFLEX Rapid Direction RNA-seq Kit (NOVA-5138-10 and NOVA-5138-11; PerkinElmer) according to the provided manual ...
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bioRxiv - Neuroscience 2023Quote: Amplified Luminescent Proximity Homogeneous Assay Screen (ALPHAScreen) was carried out utilising the cMyc detection kit and Proxiplate-384 Plus plates (PerkinElmer) as previously described (Martin et al. ...
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bioRxiv - Cell Biology 2023Quote: ... 50 µ g of lysate was used for the assessment of ATP concentration using an ATP Determination Kit (ATP lite assay, PerkinElmer) as described earlier (Miyoshi et al. ...
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bioRxiv - Biochemistry 2023Quote: ... and an aliquot from the supernatant was collected to quantify cAMP using the LANCE cAMP kit (Perkin Elmer, cat#AD0262) according to the manufacturer protocol ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... accumulation by MOR agonists was assessed using the Cisbio Homogeneous Time-Resolved Fluorescence (HTRF) cAMP Gi kit (Cisbio Assays, PerkinElmer). This kit uses a Förster Resonance Energy Transfer (FRET ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were prepared from mRNA samples using NEXTFLEX® Rapid Directional RNA-seq Kit 2.0 (Perkin Elmer, Austin, Texas, USA). Quality of library preparation was assessed on a 2100 Agilent Bioanalyzer ...
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bioRxiv - Evolutionary Biology 2023Quote: Libraries were prepared from 33ng of genomic DNA using a custom miniaturized version of the NEXTFLEX Rapid XP kit protocol (PerkinElmer) that was automated on a Sciclone NGSx liquid handler (PerkinElmer) ...
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bioRxiv - Plant Biology 2023Quote: ... RPFs were directly used as input into the NEXTflex small RNA-seq kit v3 (Perkin Elmer, cat# NOVA-5132-06) or V4 (Perkin Elmer ...
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bioRxiv - Plant Biology 2024Quote: ... the fragmented gDNA was constructed into sequencing libraries using a NEXTFLEX Rapid DNA Seq Kit (PerkinElmer, Inc., Austin, TX, USA). The full-length coding sequence of ZmHsf20 was cloned into the pFN19K HaloTag T7 SP6 Flexi vector ...
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bioRxiv - Cell Biology 2024Quote: ... using a HyBEZ® oven Probes were detected using Tyramide signal amplification (TSA) reaction (1:600 dilution) using an Alexa-flour labeled TSA kit (PerkinElmer) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... involving the use of a biotinylated secondary antibody followed by an ABC complex and Cy3-tyramide kit (Perkin Elmer, cat#: FP1046) or an Alexa secondary antibody (1:500 ...
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bioRxiv - Neuroscience 2019Quote: ... before incubated with the Fluorophore Tyramide Amplification Reagent (1:200 in amplification diluent, TSA Plus multi-fluorophore detection kit, Fluorophore: Fluorescein, PerkinElmer, NEL753001KT). Sections were washed overnight in 1x PBS at RT ...
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bioRxiv - Immunology 2021Quote: ... Products of the second-step PCR reactions were purified and analysed with Caliper Labchip for DNA samples (HT DNA High Sensitivity LabChip Kit; Perkin Elmer) prior to sequencing with the Miseq or HiSeq2500 instrument for the library representation (Illumina) ...
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bioRxiv - Genomics 2021Quote: ... All treated and RNA-extracted samples were then used to produce small libraries for sequencing using a NETFLEX small RNA-Seq kit (PerkinElmer, USA).
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bioRxiv - Neuroscience 2019Quote: ... Phosphorylation was determined by alpha-screen bead-based technology using the Amplified Luminiscent Proximity Homogeneous Assay kit (PerkinElmer, Waltham, MA, USA) and the Enspire Multimode Plate Reader (PerkinElmer ...
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bioRxiv - Plant Biology 2020Quote: ... Final library products were assessed for quality on LabChip GX using the DNA High Sensitivity Reagent Kit (Perkin Elmer, MA, USA), diluted to 2 nM concentration and pooled ...
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bioRxiv - Physiology 2021Quote: ... Plasma insulin concentrations (from tail vein samplings) were determined using an ultrasensitive mouse insulin AlphaLisa detection kit (cat number AL204C) from Perkin Elmer.
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bioRxiv - Microbiology 2020Quote: ... Stool samples underwent DNA extraction using chemical and mechanical lysis with magnetic bead-based purification via the Chemagic MSM I with the Chemagic DNA Blood Kit-96 from Perkin Elmer. Prior to extraction on the MSM-I ...
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bioRxiv - Microbiology 2022Quote: ... an equivalent of 300 ng of Gag p24 was lysed in ELISA lysis buffer containing 1% Triton (Alliance HIV-1 p24 Antigen ELISA kit, Perkin Elmer) and protease inhibitors for 30 min on ice ...
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bioRxiv - Cancer Biology 2022Quote: ... Solid tumor total nucleic acid was extracted from these tumor regions on adjacent FFPE tissue sections using Chemagic 360 sample-specific extraction kits (Perkin Elmer) and digested by proteinase K ...
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bioRxiv - Genomics 2019Quote: ... DNA libraries were made using the Illumina TruSeq Nano DNA HT kit and their size and concentration determined using LabChip DX Touch (Perkin Elmer) and Quant-iT (Life Technologies) ...
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bioRxiv - Molecular Biology 2019Quote: ... The resulting double-stranded cDNA was then used to construct an Illumina-compatible sequencing library (BioO NextFlex RNA Library Kit, Perkin Elmer). Libraries were quantified using qPCR (NEBNext Library Quantification ...
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bioRxiv - Genomics 2019Quote: ... Probes were then amplified and the HRP signal was developed using TSA Fluorescein Plus Evaluation Kit (NEL741E001KT, Perkin Elmer, Waltham, MA). Finally ...
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bioRxiv - Physiology 2019Quote: ... and stimulated with either GLP-1 or GIP for 30 minutes prior to measurement of cAMP accumulation using a LANCE® Ultra cAMP Detection Kit (PerkinElmer) in accordance with the manufacturer’s instructions and as described previously (61 ...
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bioRxiv - Neuroscience 2019Quote: ... Detection was visualized with tyramide-488 1:50 in supplied amplification buffer from the TSA-Plus in situ kit (Perkin Elmer).
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bioRxiv - Evolutionary Biology 2021Quote: ... DNA from parents and progenies was extracted from 100 mg of frozen young leaves with the Chemagic DNA Plant Kit (Perkin Elmer Chemagen ...
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bioRxiv - Genomics 2021Quote: ... the anti-DIG-HRP antibody was detected with the TSA-Cy3 substrate (1:50, TSATM PLUS Cy3 kit, NEL 745001KT, Perkin Elmer) for 10 min at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... The DNA concentration was measured with Quant-iT™ PicoGreen™ dsDNA Assay Kit after it was eluted in 400µl 10mM Tris-EDTA elution buffer (PerkinElmer). Aliquotes of DNA samples were produced with Tecan Genesis/Tecan Freedom Evo and then shipped on dry ice for genetic analyses ...
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bioRxiv - Cancer Biology 2022Quote: ... Media supernatants of the 4T1 cells in the plates were collected and immediately analyzed with ATPlite one-step luminescence assay kits (PerkinElmer, MA) for the measurement of ATP concentration in the media ...
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bioRxiv - Cell Biology 2022Quote: ... Accumulation of IP1 was measured using the IP-One Gq Homogenous Time-Resolved Fluorescence (HTRF) kit from Perkin Elmer (Waltham, MA), per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... adapter ligation and PCR were performed using the NEXTFLEX® Rapid DNA-Seq Kit 2.0 for Illumina® Platforms (PerkinElmer, UK) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... The libraries were quantified on the Perkin Elmer LabChip GX Touch with the DNA High Sensitivity Reagent kit (Perkin Elmer, CLS760672). Libraries were pooled in equimolar ratios ...
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bioRxiv - Cancer Biology 2022Quote: ... tissues were cut as 4 μm sections and automated IF was performed on a Leica Bond Rx Platform at room temperature using the PerkinElmer Opal 4-Colour Automation IHC Kit (PerkinElmer, #NEL800001KT). Tissue sections were blocked with 3% Hydrogen peroxide (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: The AlphaLISA pSTAT1 assay was performed using the AlphaLISA SureFire Ultra p-STAT1 (Tyr701) assay kit (PerkinElmer, Inc., Waltham, MA, USA). HeLa cells (CCL-2 ...
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bioRxiv - Biochemistry 2023Quote: ... Culture supernatants were harvested post-stimulation and the production of TNF-α was assessed using the AlphaLISA Immunoassay Kit (PerkinElmer, AL208C) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... Total RNA was extracted from the female Epstein-Barr virus transformed B-lymphocyte cell line GM24143 using the Chemagic™ RNA TissuelO Kit H96 (PerkinElmer) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... libraries were generated from a mixture of poly(A)+ enriched and rRNA-depleted samples using the NEXTflex® Rapid Directional qRNA-Seq Kit (PerkinElmer). Fragmentation of the RNAs was carried out enzymatically at 95°C for 10 min via the addition of NEXTflex® RNA Fragmentation Buffer ...
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bioRxiv - Pathology 2023Quote: ... as previously described [71] using Multiplex Fluorescent Reagent Kit V2 (Advanced cell Diagnostics) and TSA Plus Cyanine 3 and Cyanine 5 (Perkin Elmer) as fluorophores according to manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were prepared using 10 ng of purified PCR product with a NEXTFLEX® Rapid XP DNA-Seq Kit (PerkinElmer) barcoded using NEXTFLEX® UDI Barcodes (PerkinElmer) ...
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bioRxiv - Biochemistry 2022Quote: ... The homogeneous time-resolved fluorescence IP1 assay was used to measure intracellular IP1 accumulation according to the Cisbio IP-One Gq kit (Cisbio Bioassays, Perkin Elmer). Dose-response curves were fitted by Prism 8 (Graphpad) ...
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bioRxiv - Cancer Biology 2023Quote: ... Following an HRP conjugation step a TSA Opal 570 1:150 (Tyramide Signal Amplification Opal 4-Color Manual IHC Kit, Perkin Elmer) was linked to the IRF7 antibody previously bound to the slide overnight ...
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bioRxiv - Microbiology 2023Quote: Stool material was homogenized using TissueLyzer from Quigen and the lysate was prepared for extraction with the Chemagic Stool gDNA extraction kit (Perkin Elmer) and extracted on the Chemagic 360 Instrument (Perkin Elmer ...
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bioRxiv - Genomics 2023Quote: ... and streptavidin-conjugated Atto-425 (Bio Trend) at a concentration of 1:400 using TSA-biotin (TSA Plus Biotin Kit, Perkin Elmer). All nuclei were DAPI-stained ...
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bioRxiv - Physiology 2024Quote: ... Analysis of plasma glucagon and insulin levels were performed with the corresponding mouse plasma insulin (HTRF insulin mouse serum kit, ref: 62IN3PEF, Cisbio/Perkin Elmer) and glucagon (mouse glucagon ELISA kit ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Libraries were prepared following the NEXTflex Small RNA-Seq Kit v3 protocol provided by Perkin Elmer (Perkin Elmer, Waltham, MA, USA), starting with 200 ng of treated RNA ...
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bioRxiv - Bioengineering 2024Quote: ... These cells enabled calibration of a glucagon-dependent cAMP production assay using the LANCE™ Ultra cAMP Kit (PerkinElmer, Waltham, MA) following manufacturer instructions.
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bioRxiv - Molecular Biology 2024Quote: ... or from 10 µg total RNA from gravid adults (for analysis of protein-coding genes near dysregulated piRNAs on Chromosome IV) using the stranded NEXTFLEX Rapid Directional RNA-seq kit (Perkin Elmer). The embryonic and larval libraries represented two independent biological replicates of wild type and ebax-1(tm2321 ...
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bioRxiv - Cell Biology 2020Quote: ... The blot was washed in TTBS for 3 times and the signal was developed using Western Lightening Plus-ECL kit (PerkinElmer, Waltham, MA) and detected on HyBlot CL X-ray films (Denville).
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bioRxiv - Immunology 2019Quote: ... Wells were then washed twice and the number of adherent cells was determined using the ATPlite luminescence assay kit (PerkinElmer, Waltham, MA).
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bioRxiv - Neuroscience 2020Quote: ... Double immunofluorescence was performed using tetramethyl rhodamine labelled secondary antibody for alpha-synuclein and NSF with the fluorescein signal amplification kit (Perkin Elmer, UK). Following adequate washes to remove background fluorescence ...