Labshake search
Citations for PerkinElmer :
751 - 800 of 848 citations for Vitamin D Lumisterol CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... DNA libraries were made using the Illumina TruSeq Nano DNA HT kit and their size and concentration determined using LabChip DX Touch (Perkin Elmer) and Quant-iT (Life Technologies) ...
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bioRxiv - Molecular Biology 2019Quote: ... The resulting double-stranded cDNA was then used to construct an Illumina-compatible sequencing library (BioO NextFlex RNA Library Kit, Perkin Elmer). Libraries were quantified using qPCR (NEBNext Library Quantification ...
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bioRxiv - Genomics 2019Quote: ... Probes were then amplified and the HRP signal was developed using TSA Fluorescein Plus Evaluation Kit (NEL741E001KT, Perkin Elmer, Waltham, MA). Finally ...
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bioRxiv - Physiology 2019Quote: ... and stimulated with either GLP-1 or GIP for 30 minutes prior to measurement of cAMP accumulation using a LANCE® Ultra cAMP Detection Kit (PerkinElmer) in accordance with the manufacturer’s instructions and as described previously (61 ...
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bioRxiv - Neuroscience 2019Quote: ... Detection was visualized with tyramide-488 1:50 in supplied amplification buffer from the TSA-Plus in situ kit (Perkin Elmer).
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bioRxiv - Evolutionary Biology 2021Quote: ... DNA from parents and progenies was extracted from 100 mg of frozen young leaves with the Chemagic DNA Plant Kit (Perkin Elmer Chemagen ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Ligand-induced ERK1/2 phosphorylation was assessed using the AlphaLISA Surefire Ultra p-ERK1/2 (Thr202/Tyr204) kit (PerkinElmer, Melbourne, Australia) following the manufacturer’s protocol as previously described (23 ...
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bioRxiv - Genomics 2021Quote: ... the anti-DIG-HRP antibody was detected with the TSA-Cy3 substrate (1:50, TSATM PLUS Cy3 kit, NEL 745001KT, Perkin Elmer) for 10 min at room temperature ...
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bioRxiv - Immunology 2020Quote: We stained 5-µm-thick formalin-fixed paraffin-embedded sections from human xenograft tumors (UMSCC-1) using Opal multiplex kits (PerkinElmer/Akoya), for a panel of DAPI ...
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bioRxiv - Neuroscience 2021Quote: ... The DNA concentration was measured with Quant-iT™ PicoGreen™ dsDNA Assay Kit after it was eluted in 400µl 10mM Tris-EDTA elution buffer (PerkinElmer). Aliquotes of DNA samples were produced with Tecan Genesis/Tecan Freedom Evo and then shipped on dry ice for genetic analyses ...
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bioRxiv - Cancer Biology 2022Quote: ... Media supernatants of the 4T1 cells in the plates were collected and immediately analyzed with ATPlite one-step luminescence assay kits (PerkinElmer, MA) for the measurement of ATP concentration in the media ...
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bioRxiv - Cell Biology 2022Quote: ... Accumulation of IP1 was measured using the IP-One Gq Homogenous Time-Resolved Fluorescence (HTRF) kit from Perkin Elmer (Waltham, MA), per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... adapter ligation and PCR were performed using the NEXTFLEX® Rapid DNA-Seq Kit 2.0 for Illumina® Platforms (PerkinElmer, UK) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... The libraries were quantified on the Perkin Elmer LabChip GX Touch with the DNA High Sensitivity Reagent kit (Perkin Elmer, CLS760672). Libraries were pooled in equimolar ratios ...
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bioRxiv - Cancer Biology 2022Quote: ... tissues were cut as 4 μm sections and automated IF was performed on a Leica Bond Rx Platform at room temperature using the PerkinElmer Opal 4-Colour Automation IHC Kit (PerkinElmer, #NEL800001KT). Tissue sections were blocked with 3% Hydrogen peroxide (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: The AlphaLISA pSTAT1 assay was performed using the AlphaLISA SureFire Ultra p-STAT1 (Tyr701) assay kit (PerkinElmer, Inc., Waltham, MA, USA). HeLa cells (CCL-2 ...
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bioRxiv - Biochemistry 2023Quote: ... Culture supernatants were harvested post-stimulation and the production of TNF-α was assessed using the AlphaLISA Immunoassay Kit (PerkinElmer, AL208C) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... Total RNA was extracted from the female Epstein-Barr virus transformed B-lymphocyte cell line GM24143 using the Chemagic™ RNA TissuelO Kit H96 (PerkinElmer) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... libraries were generated from a mixture of poly(A)+ enriched and rRNA-depleted samples using the NEXTflex® Rapid Directional qRNA-Seq Kit (PerkinElmer). Fragmentation of the RNAs was carried out enzymatically at 95°C for 10 min via the addition of NEXTflex® RNA Fragmentation Buffer ...
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bioRxiv - Pathology 2023Quote: ... as previously described [71] using Multiplex Fluorescent Reagent Kit V2 (Advanced cell Diagnostics) and TSA Plus Cyanine 3 and Cyanine 5 (Perkin Elmer) as fluorophores according to manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were prepared using 10 ng of purified PCR product with a NEXTFLEX® Rapid XP DNA-Seq Kit (PerkinElmer) barcoded using NEXTFLEX® UDI Barcodes (PerkinElmer) ...
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bioRxiv - Biochemistry 2022Quote: ... The homogeneous time-resolved fluorescence IP1 assay was used to measure intracellular IP1 accumulation according to the Cisbio IP-One Gq kit (Cisbio Bioassays, Perkin Elmer). Dose-response curves were fitted by Prism 8 (Graphpad) ...
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bioRxiv - Cancer Biology 2023Quote: ... Following an HRP conjugation step a TSA Opal 570 1:150 (Tyramide Signal Amplification Opal 4-Color Manual IHC Kit, Perkin Elmer) was linked to the IRF7 antibody previously bound to the slide overnight ...
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bioRxiv - Microbiology 2023Quote: Stool material was homogenized using TissueLyzer from Quigen and the lysate was prepared for extraction with the Chemagic Stool gDNA extraction kit (Perkin Elmer) and extracted on the Chemagic 360 Instrument (Perkin Elmer ...
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bioRxiv - Genomics 2023Quote: ... and streptavidin-conjugated Atto-425 (Bio Trend) at a concentration of 1:400 using TSA-biotin (TSA Plus Biotin Kit, Perkin Elmer). All nuclei were DAPI-stained ...
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bioRxiv - Microbiology 2023Quote: Apical wash fractions collected pre- and post-infection were used to detect Lactate dehydrogenase activity and IL-8 concentrations using human LDH and IL-8 Alphalisa Kits (Perkin Elmer). ELISA Kits for the detection of human TNFα ...
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bioRxiv - Physiology 2024Quote: ... Analysis of plasma glucagon and insulin levels were performed with the corresponding mouse plasma insulin (HTRF insulin mouse serum kit, ref: 62IN3PEF, Cisbio/Perkin Elmer) and glucagon (mouse glucagon ELISA kit ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Libraries were prepared following the NEXTflex Small RNA-Seq Kit v3 protocol provided by Perkin Elmer (Perkin Elmer, Waltham, MA, USA), starting with 200 ng of treated RNA ...
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bioRxiv - Bioengineering 2024Quote: ... These cells enabled calibration of a glucagon-dependent cAMP production assay using the LANCE™ Ultra cAMP Kit (PerkinElmer, Waltham, MA) following manufacturer instructions.
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bioRxiv - Molecular Biology 2024Quote: ... or from 10 µg total RNA from gravid adults (for analysis of protein-coding genes near dysregulated piRNAs on Chromosome IV) using the stranded NEXTFLEX Rapid Directional RNA-seq kit (Perkin Elmer). The embryonic and larval libraries represented two independent biological replicates of wild type and ebax-1(tm2321 ...
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bioRxiv - Cell Biology 2020Quote: ... The blot was washed in TTBS for 3 times and the signal was developed using Western Lightening Plus-ECL kit (PerkinElmer, Waltham, MA) and detected on HyBlot CL X-ray films (Denville).
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bioRxiv - Immunology 2019Quote: ... Wells were then washed twice and the number of adherent cells was determined using the ATPlite luminescence assay kit (PerkinElmer, Waltham, MA).
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bioRxiv - Neuroscience 2020Quote: ... Double immunofluorescence was performed using tetramethyl rhodamine labelled secondary antibody for alpha-synuclein and NSF with the fluorescein signal amplification kit (Perkin Elmer, UK). Following adequate washes to remove background fluorescence ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Detection of either ERK1/2 or Akt1/2/3 phosphorylation was performed as described in the corresponding AlphaScreen SureFire kits and fluorescence measured with an EnVision plate reader (PerkinElmer, Boston, MA). Data were normalised to the response elicited upon stimulation of cells with 10% FBS.
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bioRxiv - Microbiology 2021Quote: ... 1-3ng/μl of the PCR amplifier of the HPV L1 gene was mixed with 8 μL of ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction kit version 1.1(Perkin Elmer Biosystems, USA), 2 pmol of primer ...
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bioRxiv - Microbiology 2021Quote: ... using 100 ng total RNA for library preparation with the NEXTFLEX® Small RNA-Seq Kit v3 (PerkinElmer Inc., Waltham, MA, USA), followed by library sequencing on one lane NovaSeq6000 SP v1.0 (2×50bp) ...
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bioRxiv - Microbiology 2020Quote: ... The concentration of HIV-1 Gag p24 antigen in supernatants was determined with HIV-1 p24 ELISA kit (PerkinElmer, Inc. Waltham, MA). To analyze HIV-1 Env expression ...
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bioRxiv - Microbiology 2019Quote: ... bacteria DNA was extracted using the chemagic STAR DNA BTS Kit for the automated isolation of DNA (Perkin Elmer, Wellesley, MA, USA). Quality and purity of the isolated DNA were checked by spectrophotometry on the NanoDrop (Fisher Scientific ...
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bioRxiv - Biochemistry 2019Quote: ... The RNA 5’ phosphate was removed by quick dephosphorylation kit (New England biolabs) and phosphorylated by T4 polynucleotide kinase (New England biolabs) and [γ -32P]ATP (3000 Ci/mmol) (Perkin Elmer). The samples were loaded onto 10% polyacrylamide denaturing gel (8 M urea) ...
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bioRxiv - Physiology 2020Quote: Renal tissue cAMP production following ligand stimulation was measured using the time-resolved fluorescence energy transfer (TR-FRET) based LANCE ULTRA cAMP Kit (PerkinElmer, MA, USA) in combination with an EnSight Multimode Plate Reader (Perkin Elmer ...
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bioRxiv - Neuroscience 2020Quote: ... 75 µL of supernatant from each well was collected for ATP supernatant detection using the ATPlite Luminescence Assay Kit (Perkin Elmer, 6016943) as described by the kit protocol ...
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bioRxiv - Microbiology 2021Quote: ... Viral supernatant was then used to transduce each cell line of interest for 48 h and luciferase activity was measured with Promega Steady-Glo® kit (E2550) using a Victor X3 Multilabel Reader (Perkin Elmer). Each experiment shown is representative of three independent experiments.
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bioRxiv - Developmental Biology 2021Quote: ... the RNAscope Multiplex Fluorescent Reagent Kit (v.2) and TSA Plus reagents (Perkin Elmer; 1:500 dilution for Cy3 and Cy5 dyes) or Opal dyes (Akoya Biosciences ...
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bioRxiv - Immunology 2021Quote: ... HIV-1 p24 content (produced by the backbone vector) was quantified using the Alliance p24 ELISA Kit (PerkinElmer Health Sciences, Cat# NEK050B001KT). Relative luminescence units (RLUs ...
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bioRxiv - Genetics 2020Quote: Genomic DNA was extracted from 250 mg of leaves ground to powder under liquid nitrogen using the Chemagic DNA Plant Kit (Perkin Elmer, Germany), according to the manufacturer’s instructions on a KingFisher Flex™ (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Peripheral blood samples or buffy coat extracts were extracted in Auria Biobank for all patients for DNA extraction and genomic sequencing to serve as germline reference for identifying somatic genomic aberrations using Chemagic DNA Blood Kit Special (PerkinElmer Inc., USA) and Chemagic 360 instrument (PerkinElmer Inc. ...
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bioRxiv - Immunology 2024Quote: ... 1:1 mixed with maleate buffer (R2a from the kit)) was added and incubated at room temperature and measured kinetically on an Envision microplate reader (PerkinElmer, Waltham, MA) at 340 nm for 20 min ...
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bioRxiv - Neuroscience 2023Quote: ... cAMP levels were measured with an Amplified Luminescent Proximity Homogeneous Assay (ALPHA) Screen cAMP assay kit as instructed by the manufacturer (PerkinElmer, Waltham, MA).
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An updated C. elegans nuclear body muscle transcriptome for studies in muscle formation and functionbioRxiv - Genetics 2023Quote: ... The libraries were prepared from the ribosomal RNA depleted samples using the Nextflex Rapid Directional RNAseq Kit (PerkinElmer Catalog # NOVIA-5138-08) as per the manufacturer’s protocol and sequenced on the Nextseq 500 instrument (Illumina ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Messenger RNA was isolated from 150 ng of total RNA using a Nextflex Poly-A Selection kit (Perkin Elmer; Waltham, MA, USA). cDNA libraries were prepared using a Nextflex Rapid Directional RNA 2.0 kit ...