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Citations for PerkinElmer :
701 - 750 of 770 citations for R 4 Benzyl 2 2 diphenylphosphino benzyl 4 5 dihydrooxazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... cooled to room temp (5 min) and absorbance was read at 432 nm on a Wallac 1420 VICTOR Multilabel reader (Perkin Elmer). Formaldehyde standards were prepared daily from 1 M formaldehyde stock solutions and a standard curve was alongside all sample measurements.
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bioRxiv - Bioengineering 2020Quote: ... Lungs were incubated for 5 minutes and then placed onto black paper and imaged with an IVIS Spectrum CT (Perkin Elmer). Lung luminescence was then quantified using Living Image software (Perkin Elmer).
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bioRxiv - Microbiology 2020Quote: ... Probes corresponding to the 5’ and 3’ flanks of the laeA gene in each strain were labeled using [a32P] dCTP (PerkinElmer, USA) following the manufacturer’s instructions.
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bioRxiv - Plant Biology 2020Quote: ... was incubated with oligonucleotide probes (0.01 pmol-10 fmol) 5’-radiolabeled with [y-32P]ATP (5000 Ci/mmol; PerkinElmer Life Science). Different dsDNA structures were prepared by annealing the radiolabeled sense oligonucleotides with a twofold excess of unlabeled complementary oligonucleotide and purified on non-denaturing polyacrylamide gels ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were pre-treated with anisomycin (100 ug/ml) for 5 min to inhibit cytoplasmic translation then pulsed with 200 uCi/ml 35S Met-Cys (EasyTag-Perkin Elmer). In chase experiments ...
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bioRxiv - Biochemistry 2021Quote: ... Thirty μL of tissue homogenate supernatant were mixed with 370 μL of the oxidation reaction mixture containing 7% BSA/5 mM palmitate/0.01 μCi/μL 14C-palmitate (PerkinElmer, Waltham, MA) and incubated at 37 °C for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... cooled to room temp (5 min) and absorbance was read at 432 nm on a Wallac 1420 VICTOR Multilabel reader (Perkin Elmer). Formaldehyde standards were prepared daily from 1 M formaldehyde stock solutions and a standard curve was generated in parallel with samples at each measurement (timepoint).
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bioRxiv - Cell Biology 2020Quote: ... subconfluent HDLECs cultured in 12-well plates were incubated with 1 ml per well EBM2 medium (containing appropriate amounts of serum and supplement) with [5-3H]-glucose (Perkin Elmer) for 2-3 hours ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the samples were diluted into 5% nitric acid and 1% hydrochloric acid and tested on the PerkinElmer NexION 300D (PerkinElmer, Inc.). A calibration curve with known Au concentrations was prepared and the Au concentration was determined according to absorbance values ...
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bioRxiv - Biochemistry 2021Quote: ... cells were starved for cysteine and methionine for 15-30 min and pulse labeled for 5 min with 55 µCi/ 35-mm dish of Easytag express 35S protein labeling mix (Perkin Elmer). Where indicated (+DTT) ...
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bioRxiv - Immunology 2020Quote: We stained 5-µm-thick formalin-fixed paraffin-embedded sections from human xenograft tumors (UMSCC-1) using Opal multiplex kits (PerkinElmer/Akoya), for a panel of DAPI ...
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bioRxiv - Microbiology 2020Quote: ... per well and gently mixing by manually swirling the plate (a 5 min incubation prior to reading the plate is recommended) before reading luciferase activity on an Envision plate reader (Perkin Elmer). Due to time sensitivity of the luciferase signal ...
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bioRxiv - Molecular Biology 2023Quote: Glassware and plasticware used for RNA gel electrophoresis were soaked in 10% bleach for 5 minutes before washed by COUNT-OFF™ Surface Cleaner (PerkinElmer) and liberally rinsing in double-distilled water ...
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bioRxiv - Microbiology 2023Quote: ... collected water samples (50 mL) were immediately spiked with 5 µCi of NaH14CO3 (Perkin Elmer, specific activity 56 mCi mmol−1). The samples were incubated for 24 h under in situ natural illumination ...
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bioRxiv - Pathology 2023Quote: ... as previously described [71] using Multiplex Fluorescent Reagent Kit V2 (Advanced cell Diagnostics) and TSA Plus Cyanine 3 and Cyanine 5 (Perkin Elmer) as fluorophores according to manufacturer’s recommendations ...
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bioRxiv - Immunology 2023Quote: ... Iodine-125 (125I) labeling was performed by incubating DTPA-bsAbs in iodogen coated Eppendorf tubes with Na125I (10−5 M NaOH, Perkin Elmer) in 0.5 M phosphate buffer (pH 7.4 ...
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bioRxiv - Neuroscience 2022Quote: ... Following HRP development, TSA Plus Cyanine3 (Cy3, for Trpa1) and Cyanine 5 (Cy5, for Oprk1) fluorophores (Perkin Elmer, 1:1500 dilution) were applied for 30min at 40°C ...
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bioRxiv - Molecular Biology 2022Quote: ... and a DNA loading control also terminating in 20 adenosines (IDT) (Supplementary Table S2) were 5’ labelled with [γ-32P]-ATP (PerkinElmer) using T4 poly-nucleotide kinase (NEB ...
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bioRxiv - Physiology 2022Quote: ... 100 ng of PCR product was used in an in vitro transcription reaction with T7 polymerase (antisense) in the presence of 35S-uridine 5 triphosphate (Perkin Elmer) for radioactive in situ hybridisation.
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bioRxiv - Immunology 2022Quote: ... The plate was shaken for 5 minutes and counts were read by a Victor X5 time-resolved fluorometry plate reader (Perkin Elmer).
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bioRxiv - Immunology 2022Quote: ... The plate was shaken for 5 minutes and counts were read by a time-resolved fluorometry plate reader Victor X5 (Perkin Elmer).
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... Proteins were labelled for 5 mins (for soluble proteins) or 15 mins (for membrane proteins) with 30 µCi of Express Protein Labelling Mix (Perkin Elmer) per sample at their restrictive temperature ...
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bioRxiv - Systems Biology 2023Quote: ... 0.5 mg/ml was added 1-hour prior to medium collection and fluorescence intensities were analyzed with plate reader (PerkinElmer 2030).
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bioRxiv - Molecular Biology 2023Quote: ... 50 ng of heat-denatured DNA was incubated for 30 min at 37°C in a total volume of 25 μl containing random primer and 5 μl of [α-32P]-dCTP (3,000 Ci/mmol, 10 mCi/ml; Perkin Elmer). After the reaction ...
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bioRxiv - Biochemistry 2023Quote: ... Each binding reaction was allowed to reach equilibrium at 30 °C for 5 min prior to fluorescence polarization measurements in a Victor X5 Multilabel Plate Reader (Perkin Elmer). Each Kd measurement was calculated according to our previous work (45) ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mL fractions containing soluble inositol phosphates were mixed with 3 mL scintillation cocktail (Ultima-Flo AP) and counted for 5 min in a liquid scintillation counter (Tri-Carb 2910 TR, Perkin Elmer). The lipid inositol extract was counted in a liquid scintillation counter ...
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bioRxiv - Biochemistry 2022Quote: ... then centrifuged at 61 g for 10 seconds and the data were acquired in a Victor X 5 plate reader (Perkin Elmer) with an excitation wavelength of 480 nm and an emission wavelength of 535 nm ...
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bioRxiv - Molecular Biology 2022Quote: ... Then for the 6xHIS-tagged HAT module a 10 µL mixture of 5 µg/mL AlphaLISA Nickel chelate acceptors beads (PerkinElmer #AL108) and 10 µg/mL AlphaScreen donor beads (PerkinElmer #6760002) ...
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bioRxiv - Microbiology 2022Quote: ... in the ratio of 5:95 and analyzed at mid-IR region (400–4000 cm−1) by FTIR using Spectrum GX (PerkinElmer, USA).
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bioRxiv - Microbiology 2022Quote: ... Probes corresponding to the 5’ and 3’ flanks of the laeA gene in each strain were labeled using [α32P] dCTP (PerkinElmer, USA) following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... then each well was incubated for 30 min at 37 °C with 5 μl/well EXPRE35S35S Protein Labeling Mix (PerkinElmer NEG072002MC), after which cells were lysed in RIPA lysis buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Immunology 2023Quote: ... TZM-bl target cells were seeded at a density of 5 × 103 cells/well in 96-well luminometer-compatible tissue culture plates (Perkin Elmer) 24 h before infection ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were washed 3 times for 5 min in TBS-T buffer and prior to chemiluminescence detection using Western Lightning chemiluminescent substrate (Perkin Elmer). Chemiluminescence signal was detected with an ECL-Imager (INTAS ...
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bioRxiv - Microbiology 2024Quote: ... 5 ml cultures were grown in the presence of [32P]-phosphate (final concentration of 1 µCi ml-1; Perkin Elmer NEX060001MC) overnight ...
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bioRxiv - Biophysics 2024Quote: ... A six-point standard curve from 200 ppb to 1 ppb (200, 100, 50, 10, 5, 1) was prepared from elemental standards (Perkin Elmer), plus a 0-ppb blank ...
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bioRxiv - Microbiology 2020Quote: Purified Caulobacter ParB-His6 or TetR-His6 (final concentration: 25 μM) were incubated with 5 nM radiolabeled P32-α-CTP (Perkin Elmer), 30 μM of unlabeled cold CTP (Thermo Fisher) ...
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bioRxiv - Plant Biology 2021Quote: ... Seedlings were metabolically labelled with radioactive phosphate by incubating them overnight for ~16-20 hrs with 10 μL (5-10 μCi) carrier-free 32P-PO43- (32Pi; PerkinElmer, The Netherlands) in labelling buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were fixed with 2% PFA at RT for 30 min and stained with the Hoechst 33342 dye (1 μg/ml) at 37 °C and 5% CO2 before imaging with the Operetta™ high content imaging system (PerkinElmer Inc.). Forty fields were acquired from each well using appropriate filter sets ...
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bioRxiv - Molecular Biology 2020Quote: 100 pmol of WT or mutant RNA oligonucleotides (synthesized by Integrated DNA Technologies, Inc.) were 5’- end labeled with [γ32P] ATP (Perkin Elmer, NEG035C001MC) and T4 polynucleotide kinase (New England Biolabs ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were finally washed three times with PBST for 5 min and protein levels were visualized by enhanced chemiluminescence (Perkin Elmer, NEL104001EA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Tumors were isolated from mice and sectioned into 5 micrometer sections for staining with the desired markers (below) using Tyramide Signal Amplification (PerkinElmer, Waltham MA) per manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2021Quote: ... The colonies were grown in 160 μL of TAP medium for 5 days in black clear bottom 96 well plates (CellCarrier-96 Black, 6005550, PerkinElmer Inc, USA), sealed with a paper tape ...
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bioRxiv - Neuroscience 2023Quote: ... produced in Sf9 cells, and ROCK1 (Carna Biosciences, Natick, MA; 5 nM) using the Lance-FRET kinase assay system (PerkinElmer, Waltham, MA). Other human recombinant PKCs (SignalChem Biotech ...
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bioRxiv - Developmental Biology 2024Quote: ... Plates (with lid on) were placed in the Opera Phenix High-Content Screening System (Perkin Elmer; prewarmed to 37C and 5% CO2). Images were collected every 30 min for 24 hours.
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bioRxiv - Immunology 2023Quote: ... Fluorescent signals were monitored in real-time with a microplate reader for 24 h within a tissue culture incubator (37 °C, 5 % CO2) on black OptiPlate 96-well (PerkinElmer, MA, USA) under 20x objective ...
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bioRxiv - Biophysics 2023Quote: ... stand and a 40 nt oligo-dT at the 5′ end was labeled at the 3′ terminus with [α-32P] dCTP (Perkin Elmer) and annealed to the single-stranded DNA M13mp18(24) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Determination of Gαi and Gαs selective signaling at the 5-HT1AR was performed applying an IP accumulation assay (IP-One HTRF®, PerkinElmer, Rodgau, Germany) according to the manufacturer’s protocol and in analogy to previously described protocols (15 ...
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bioRxiv - Immunology 2023Quote: ... Hybridization signals were detected by fluorescent signal using peroxidase-based Tyramide Signal Amplification-Plus Fluorescein or TSA Plus Cyanine 5 (Perkinelmer, Shelton, CT). Finally ...
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bioRxiv - Systems Biology 2024Quote: ... The NPCs were cultured in a 12-well dish to confluency in NPC media and then dissociated using Accutase for 5 minutes at 37°C to single cell resolution and plated on 5 µg/mL human laminin and 5µg/mL fibronectin-coated 384 well plates (Perkin Elmer Cat #6057308) at 12,000 cells/well in NPC media and 10 µM ROCKi ...
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bioRxiv - Immunology 2024Quote: ... Monocytes from five healthy donors were resuspended in an RPMI medium at 5 x 105 c/ml and distributed into the wells (100 μL) of white opaque 96-well microplates (Perkin Elmer, PKE_6005299). Macrophage cell cultures were generated by adherence of monocytes to cell culture plasticware as previously described (16) ...