Labshake search
Citations for PerkinElmer :
701 - 750 of 759 citations for L β Homo β 2 hydroxyphenyl glycine; R 3 Amino 3 2 hydroxyphenyl propanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Samples were amended with 100 nmol of leucine L-1 (Perkin Elmer, specific activity 156 Ci mmol-1) and incubated for 4 h in the dark under ambient surface sea water temperature (∼19 °C) ...
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bioRxiv - Molecular Biology 2023Quote: ... CDCA7e or HELLS wildtype or interface mutants were expressed and radiolabeled with EasyTagTM L-[35S]-Methionine (Perkin Elmer) using the TnT Coupled Reticulocyte Lysate System (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... the rabbit reticulocyte lysate master mix was supplemented with EasyTag L-35-Methionine (4% final reaction volume; PerkinElmer). Canine rough pancreatic microsomes (cRMs ...
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bioRxiv - Physiology 2020Quote: ... The integrity of resultant cDNA was assessed using a LabChip GX Touch 24 Nucleic Acid Analyzer as per manufacturer’s instructions (Perkin Elmer).
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bioRxiv - Immunology 2022Quote: ... Quality and quantity were checked using a Caliper LabChip GX Touch HT Nucleic Acid Analyzer (PerkinElmer, Waltham, MA 02452 USA). The PCR product library was quantitated and subjected to sequencing on an Illumina MiSeq sequencer (Illumina ...
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bioRxiv - Immunology 2022Quote: ... using the RNA Assay and DNA 5K/RNA/CZE HT Chip on a LabChip GX Touch HT Nucleic Acid Analyzer (PerkinElmer). TruSeq Stranded mRNA Library Prep kit (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... DNA quantification and quality check was done using a LabChip GX Touch HT Nucleic Acid Analyzer (PerkinElmer, Waltham, MA, USA). Libraries were prepared as detailed on the Illumina® support website (http://support.illumina.com ...
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bioRxiv - Biochemistry 2023Quote: ... Protein biosynthesis rates were determined by measuring the incorporation of radioactive methionine in auranofin-treated and untreated samples after protein precipitation with 20 % (w/v) trichloro acetic acid using a scintillation counter (Tri-Carb 2800TR, PerkinElmer). 55 µg of protein from pulse-labeled samples for analytical gels and 300 µg of protein from unlabeled samples for preparative gels were rehydrated into IPG strips (24 cm ...
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bioRxiv - Microbiology 2024Quote: ... Quality and quantity were checked using a Caliper LabChip GX Touch HT Nucleic Acid Analyzer (PerkinElmer, Waltham, MA 02452 USA). The PCR product library was quantitated and subjected to sequencing on an Illumina MiSeq sequencer (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... Quality and quantity were checked using a Caliper LabChip GX Touch HT Nucleic Acid Analyzer (PerkinElmer, Waltham, MA 02452 USA). The PCR product library was quantitated and subjected to sequencing on an Illumina MiSeq sequencer (Illumina ...
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bioRxiv - Neuroscience 2020Quote: ... The receptor autoradiography procedure was performed using a linear V1a-R antagonist [125I]-d(CH2)5(Tyr[Me])-AVP (Perkin Elmer, USA) or a linear OXTR antagonist [125I]-d(CH2)5[Tyr(Me)2-Tyr-Nh2]9-OVT (Perkin Elmer ...
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bioRxiv - Cell Biology 2021Quote: ... after which Stop & Glo was added to measure renilla luciferase activity (R-luc) on an EnSpire Multimode Plate Reader (Perkin Elmer, USA). F-luc was normalised to R-luc to account for transfection efficiency ...
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bioRxiv - Neuroscience 2024Quote: ... and 50 µl of radioligand diluted in binding buffer ([3H]-N-methylspiperone: 0.4 nM final concentration, Perkin Elmer, Waltham, MA; [3H]-(R)-(+)-7-OH-DPAT ...
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bioRxiv - Biophysics 2021Quote: ... HEK293 TauRD-P301S-CFP/YFP expressing biosensor cells were plated in poly-L-lysine coated 96-well plates (PerkinElmer) at a density of 20000 cells/well and subsequently transfected with pre-incubated mixtures of tau seeds/CAP1 ...
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bioRxiv - Microbiology 2020Quote: ... programmed with 1 µg DNA/50 µL and labeled with 20 µCi of L-[35S]-methionine (EasyTag, Perkin Elmer). Reactions were incubated at 30 °C for 30 min before adding L-methionine (1 mM final ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... suspended cortical cells were seeded in poly-L-lysine-coated 24- or 96-well plates (View Plate 96, PerkinElmer) at 250000 or 60000 cells/well ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HEK293T-ACE2-TMPRSS2 cells were cultured in poly-L-1ysine coated 96-well plates (ViewPlate 96, PerkinElmer Life Sciences). Next day ...
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bioRxiv - Plant Biology 2020Quote: ... Two 100-μl fractions were collected in 50 μl of 1.5 M Tris-HCl (pH 8.0) and 1 ml of Ultima Gold LLT scintillation cocktail (Perkin Elmer). Radioactivity was measured using a liquid scintillation analyser (Packard).
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bioRxiv - Molecular Biology 2024Quote: The coupled transcription-translation reaction was performed using PUREfrex v1.0 (GeneFrontier) in the presence of 35S-methionine {EasyTag L-[35S]-Methionine (PerkinElmer)} at 37 °C ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were then treated for 10 minutes with indicated compounds before addition of 20 μCi of 32P-ortho-phosphoric acid (Perkin Elmer). Cells were incubated for 1 hour with media containing drugs and 32P-ortho-phosphoric acid ...
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bioRxiv - Cancer Biology 2022Quote: ... Solid tumor total nucleic acid was extracted from these tumor regions on adjacent FFPE tissue sections using Chemagic 360 sample-specific extraction kits (Perkin Elmer) and digested by proteinase K ...
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bioRxiv - Plant Biology 2020Quote: The non-polar phase containing non-polar metabolites which included fatty acids was subjected to gas chromatography-flame ionization detection (GC-FID) (Clarus 500, Perkin Elmer). The non-polar phase was dried for fatty acid methyl esterification based on Yuan (2016) ...
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bioRxiv - Immunology 2023Quote: ... cells were isolated ex-vivo and then incubated for six hours with 0.5 μCi 14C-palmitic acid (Perkin Elmer Cat# NEC075H050UC) in the following media ...
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bioRxiv - Synthetic Biology 2020Quote: ... Radioactively labelled protein samples were then precipitated using trichloroacetic acid (TCA) and their radioactive counts measured by liquid scintillation using a MicroBeta2 (PerkinElmer, Waltham, MA) to quantify soluble and total luciferase yields as previously reported (Jewett et al. ...
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bioRxiv - Cell Biology 2020Quote: ... and medium was exchanged for Leibovitz L-15 CO2-independent medium for imaging at 37°C on a spinning disc confocal system (Ultraview Vox, PerkinElmer) with a 100 × 1.4 NA oil-immersion objective ...
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bioRxiv - Biochemistry 2021Quote: ... 150 μl of the supernatant was carefully transferred and radioactivity was measured in a liquid scintillation counter (Tri-Carb 3110TR, PerkinElmer). All samples were measured in triplicate ...
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bioRxiv - Microbiology 2020Quote: ... 200 μCi (7.4 MBq) [35S]-methionine/L-[35S] cysteine using EasyTag™ EXPRESS35S Protein Labeling Mix (PerkinElmer, Groningen, the Netherlands) was added to the medium ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were cultured in α-MEM containing media containing 2μM Glutamine and 4 mCi/mL L-[3,4-3H(N)]-Glutamine (PerkinElmer, NET551250UC). GLS activity was terminated by washing cells with ice cold HBSS for three times followed by scaping cells with 1 mL ice cold milliQ water ...
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bioRxiv - Microbiology 2020Quote: ... The dissolution kinetics of L-Ag NPs in mLB was measured by inductively coupled plasma optical emission spectrometry (ICP-OES) (Avio 200, PerkinElmer). All measurements were performed in duplicate ...
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bioRxiv - Microbiology 2022Quote: ... and at indicated times were washed and incubated for 30 mins in methionine-free DMEM before adding 50μCi of L-[35S]-methionine (Perkin Elmer) for a further 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... suspended cortical cells from each embryo were seeded in poly-L-lysine-coated 24-well plates (View Plate 96, PerkinElmer) at 250000 cells/well ...
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bioRxiv - Microbiology 2024Quote: ... Of each sample three replicates and two formaldehyde-fixed (final conc. 1.85 %) adsorption blanks were spiked with [methyl-3H]-thymidine and L-[14C(U)]-leucine (Perkin Elmer) at saturating concentrations of 20 nM and 150 nM ...
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bioRxiv - Immunology 2024Quote: ... [3H]-thymidine was added at 0.5μl (∼0.5 μCi) per well and plates incubated for 6 h before cells were harvested to UniFilter plates (Perkin Elmer) using a Tomtec 96 Harvester ...
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bioRxiv - Immunology 2022Quote: ... was added to each well (100 µl/well) and the relative light units (RLU) of luciferase activity was measured using a VICTOR X4 multiplate reader (PerkinElmer).
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bioRxiv - Systems Biology 2022Quote: ... 100 μl were transferred to a white 96-well microplate and luminescence was measured using on an EnVision plate reader (PerkinElmer).
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bioRxiv - Genomics 2023Quote: ... at a total concentration equivalent to 0.8 μM nucleosomes and 5.0 μM 14C-SAM (S- [methyl-14C]-adenosyl-l-methionine (PerkinElmer, no. NEC363050UC)) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 0.05□μl of 5□mM stock compounds in DMSO were dispensed into assay microplates with a Janus 384 MDT NanoHead (PerkinElmer). Final concentration of the screening compounds in the assay was 16LμM ...
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bioRxiv - Microbiology 2023Quote: Brine samples BP3 and BP4 were spiked with L-[4,5-3H] leucine (Perkin Elmer, specific activity 100 Ci mmol-1) at a final concentration of 20 nmol leucine L-1 (Kirchman et al. ...
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bioRxiv - Cell Biology 2024Quote: ... and 20 µL of solution (10,000 cells) was plated into the bottom of 384-well CellCarrier Ultra tissue culture plate (PerkinElmer; 6057300) pre-spotted with compounds (10 µM final concentration) ...
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bioRxiv - Biochemistry 2020Quote: ... 20 μl of the supernatant was spotted in a scintillation tube and upon the addition of Ultima Gold scintillation liquid (Perkin Elmer) the signal was measured by scintillation counter.
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bioRxiv - Cell Biology 2021Quote: ... 90 μL of the cell suspension was also plated in duplicate into wells of a 96-well black-walled plate (PerkinElmer OptiPlate), and Venus fluorescence was determined using an excitation wavelength of 485 nm (5 nm bandwidth ...
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bioRxiv - Molecular Biology 2022Quote: ... 1mM PMSF, 100mM KCl and 1 mCi of [3H]AdoMet (S-adenosyl-L-[methyl-3H] methionine, 80 Ci/mmol; SAM) (Perkin Elmer) or 0.15mM unlabeled S-adenosyl methionine for 45 min at 30°C ...
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bioRxiv - Molecular Biology 2020Quote: Purified protein complexes were incubated at room temperature with a histone substrate at a final concentration of 500nM and approximately 500nM radioactive SAM (Adenosyl-L-methionine S-methyl3H from PerkinElmer (NET155H250UC)) in methyltransferase buffer (10% glycerol ...
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bioRxiv - Microbiology 2020Quote: ... rabbit reticulocyte lysate was incubated with 1μg of the appropriate expression plasmid and 2mL of [35S] L-methionine (1,000 Ci/mMol at 10mCi/mL) (Perkin Elmer, NEG709A) for 90 minutes at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Analytical estimation of the stock CoCl2 solution concentration (100 mmol L−1) was performed employing inductively coupled mass spectrometry (Perkin Elmer Avio 200 optical emission spectrometer ...
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bioRxiv - Microbiology 2024Quote: ... and the uptake of 14C(U) l-arginine was measured in the bacterial lysates using a liquid scintillation counter (Perkin Elmer).
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bioRxiv - Microbiology 2024Quote: ... gingivalis wild-type strain was incubated in 5 mL of TSB or TSBNH (a nutritionally limited TSB media that lacks hemin) media with or without radiolabeled L-[14C(U)] Glutamate (1.0 mCi/mmol, Perkin Elmer # NEC290E050UC) at 37°C under anaerobic condition ...
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bioRxiv - Cancer Biology 2020Quote: ... proteins were first labeled for 48 h with 0.25 μM Ci ml-1 L-[U-14C] VALINE (Perkin Elmer, Waltham, MA, USA) in GIBCO-RPMI 1640 medium (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... were measured using a radiometric assay that detects transfer of a [3H] methyl from the radio-labeled cofactor S-[Methyl-3H]-adenosyl-L-methionine (SAM, PerkinElmer, cat. NET155001MC) to a biotinylated peptide Akt1-K64 (QCQLMKTERPRPNTFIIRCLQW ...
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bioRxiv - Bioengineering 2022Quote: ... PEG-maleimide was reacted with 1 mol equivalence of calpain substrate peptide and 1 mol equivalence of L-cysteine-functionalized VivoTag S-750 (PerkinElmer, Boston, MA), followed by quenching with an excess of L-cysteine ...