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Citations for PerkinElmer :
701 - 750 of 1044 citations for 3 2 5 Dioxoimidazolidin 4 yl propanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed with PBS and then stored in PBS at 4°C until imaged in the Opera Phenix (PerkinElmer) at 40x magnification and images analysed using a Harmony software to assess protein expression and morphology (see supplementary).
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bioRxiv - Molecular Biology 2022Quote: ... RNA samples were separated on a 7 M urea denaturing polyacrylamide gel at 4°C at 100V and transferred onto a charged nylon transfer membrane (PerkinElmer), followed by cross-linking using a UV Stratalinker and drying at 80°C on a Gel Dryer (Bio-Rad).
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bioRxiv - Molecular Biology 2024Quote: ... membranes were blocked with 5% BSA in Tris / HCl-buffered saline / 0.05% Tween (TBST) for 24 h at 4 °C and afterwards membranes were incubated for 1 h with HRP-Streptavidin (PerkinElmer; #NEL750001EA ...
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bioRxiv - Neuroscience 2024Quote: ... since this was when significant peripheral neuropathy first became detectable11.Tumor growth was monitored weekly for up to 4 weeks by bioluminescent imaging using an IVIS Lumina XR (PerkinElmer), as described previously11 ...
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bioRxiv - Microbiology 2021Quote: ... Luciferase activity was read approximately 2 min later using a Wallac Victor3 plate reader (Perkin Elmer). 50% ADCC titers were estimated as previously described for virus neutralization assays (41) ...
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bioRxiv - Cell Biology 2021Quote: ... and 500 µM ATP (with 2 μCi of [γ-32P] ATP added per reaction; PerkinElmer BLU502Z250UC). Phosphorylated proteins were separated on 10% SDS-PAGE gels unless stated otherwise ...
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bioRxiv - Physiology 2020Quote: ... Cells were incubated with 50 μM 2DG containing 0.25 μCi 3H-2-deoxyglucose (2DG, Perkin Elmer) for 5 min and washed three times with cold PBS ...
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bioRxiv - Biochemistry 2021Quote: ... Isolated cardiac myocytes were incubated with 0.1 mM [1,3-3H]glycerol (2 μCi/dish, Perkin Elmer) or 0.1 μM [1-14C]linoleic acid (2 μCi/dish ...
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bioRxiv - Cell Biology 2024Quote: ... The organs were harvested and imaged using the IVIS Lumina XRMS Series 2 instrument (PerkinElmer, USA) to assess the biodistribution of mannose ...
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bioRxiv - Neuroscience 2023Quote: ... Isotope content was determined in 2 ml Ultima Gold™ XR scintillation liquid (6013119, Perkin Elmer) using the Tri-Carb 2900TR Liquid Scintillation Analyzer (Packard) ...
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bioRxiv - Microbiology 2024Quote: ... The fluorescence intensity of 2 × 108 labeled cells was measured using an ARVO X3 apparatus (PerkinElmer) after 1 h of labeling ...
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bioRxiv - Physiology 2024Quote: ... Cells were then incubated with 0.5 μCi [3H]-2-deoxy-D-Glucose (cat. no. NET328A001MC, PerkinElmer) and 0.1 μM 2-deoxy-D-Glucose (2DG ...
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bioRxiv - Microbiology 2021Quote: ... The volumes of internalised DNA in GFP expressing bacteria were estimated by 3-D image analysis performed using Volocity software (Perkin Elmer, Waltham, USA).
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bioRxiv - Cancer Biology 2020Quote: ... containing 10% BSA and 3 µl [35S] EasyTag Express protein labelling mix (7 mCi, 259 MBq, 1175 Ci/mmol in 632 ml; Perkin Elmer, Espoo, Finland). After 2 h of incubation at 37 °C cells were washed twice with PBS ...
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bioRxiv - Cell Biology 2021Quote: ... 41.3 Ci mmol−1) and radiocarbon-labelled serotonin binoxalate (14C-serotonin; 54.0 mCi mmol−1) were purchased from Perkin Elmer (Waltham, Massachusetts, USA). Serotonin creatinine sulphate ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Donor and acceptor emission were measured at 3 min intervals (during a total of 66 min) at 37°C with an EnVision 2014 Multilabel Reader (PerkinElmer, Waltham, MA, USA) using a 340/60 nm excitation filter and 520/8 nm (acceptor ...
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bioRxiv - Developmental Biology 2020Quote: ... RNA hybridization were performed on dechorionated 20 hpf embryos with the tyramide amplification kit (TSA Plus Cyanine 3 System, Perkin Elmer, Boston, MA). Briefly ...
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bioRxiv - Immunology 2020Quote: ... often from an ImmPRESS® polymer detection kit followed by colour development using a tyramide signal amplification system kit with cyanine (Cy)3 or fluorescein (1:50 dilution; PerkinElmer, Waltham, MA, USA). For detection of the second antigen in dual immunofluorescence ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were generated in 3 dimensions and minimized by applying the MM2 energy-minimization method in ChemBio3D (ChemDraw® Professional v20, PerkinElmer Informatics, Inc). The minimized structures were overlaid by steric fields and the similarity was calculated in Discovery Studio Visualizer v21.1 (Dassault Systèmes Biovia Corp).
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bioRxiv - Cell Biology 2023Quote: ... Colorimetric or fluorimetric quantifications of the respective readouts were done in a multiwell plate reader (Wallac Victor 3 V, PerkinElmer, Waltham, MA, USA) and conducted in white 96-well plates.
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bioRxiv - Cancer Biology 2023Quote: ... culture plates were incubated for 3 days after which they were imaged with an Operetta CLS high-content analysis system (Perkin Elmer, LIVE configuration) with the incubation chamber set to 37 °C and 5% CO2 ...
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bioRxiv - Immunology 2020Quote: ... Plates were stored in the dark at 4°C prior to imaging using a 40x objective mounted on an Operetta High-Content Imaging System (Perkin Elmer) controlled by Harmony 4.1 software.
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bioRxiv - Cell Biology 2021Quote: ... diluted in buffer A to a final concentration of 4 nM) was added to a 384-well microplate (OptiPlateTM-384 Plus, Perkin Elmer) followed by the addition of 15 μL diluted proteins and 5 μL of the GFP antibody-conjugated acceptor beads (5x) ...
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bioRxiv - Biophysics 2020Quote: ... 100 nM hNatB was mixed with a saturating concentration of “MDVF” peptide substrate (500 μM) and varying concentrations (1.95 μM to 1 mM) of acetyl-CoA (14C-labeled, 4 mCi mmol−1; PerkinElmer Life Sciences) for 10-minute reactions ...
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bioRxiv - Developmental Biology 2022Quote: ... The sections were prehybridized and hybridized at 45°C for 4 hours in 50% (Vol/Vol) formamide hybridization buffer containing 35S-labeled antisense RNA probes (Perkin Elmer). RNase A-resistant hybrids were detected by autoradiography ...
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bioRxiv - Plant Biology 2022Quote: ... 5-mm root segments away from the apical 2 mm root tip and 2 mm away from the root-shoot junction were carefully cut and soaked overnight in 4 ml of liquid scintillation fluid (Ultima Gold, PerkinElmer, USA), and the radioactivity was measured with a scintillation counter (model LS6500 ...
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bioRxiv - Physiology 2020Quote: ... Each filter was then placed in a Sterilin™ scintillation vial and radioactivity determined by: addition of 4 mL of Ultima Gold XR liquid scintillant (PerkinElmer), overnight incubation at room temperature and the retained radioactivity determined using a Beckman Coulter LS 6500 Multi-purpose scintillation counter (Beckman Coulter Inc. ...
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bioRxiv - Neuroscience 2023Quote: ... of incubation in an isotope solution containing rubidium (86Rb+) (1 μCi/ml, 022-105721-00321-0001, POLATOM) and 3H-mannitol (4 μCi/ml, NET101, Perkin Elmer). 86Rb+ acts as a K+ congener ...
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bioRxiv - Neuroscience 2023Quote: ... Thawed serum samples were diluted with 1xPBS 1:4 and assayed in triplicates using a fluorometer (PerkinElmer, VICTOR3V 1420 Multilabel counter) with excitation/emission of 485 nm / 535 nm (25 nm band-pass filter) ...
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bioRxiv - Cancer Biology 2022Quote: ... tissues were cut as 4 μm sections and automated IF was performed on a Leica Bond Rx Platform at room temperature using the PerkinElmer Opal 4-Colour Automation IHC Kit (PerkinElmer, #NEL800001KT). Tissue sections were blocked with 3% Hydrogen peroxide (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... Transcription was initiated with addition of 12 pmol rrnBP1 PCR promoter fragment pre-mixed with 1 mM ATP and 4 μCi α-[32P]-CTP (3,000 Ci mmol−1; Perkin Elmer) at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... at 42 °C for 30 min followed by hybridization overnight at 42°C with 4 pmol probes (Table S3) that were radiolabeled using [γ-32P] ATP (PerkinElmer) and T4 Polynucleotide kinase (New England Biolabs) ...
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bioRxiv - Cancer Biology 2023Quote: ... Following an HRP conjugation step a TSA Opal 570 1:150 (Tyramide Signal Amplification Opal 4-Color Manual IHC Kit, Perkin Elmer) was linked to the IRF7 antibody previously bound to the slide overnight ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cells were incubated for four hours at 4 °C in the presence of benzazepine-red nonpeptide vasopressin antagonist (BZ-DY647, PerkinElmer Revvity) at various concentrations ranging from 10-10 to 10-7 M ...
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bioRxiv - Molecular Biology 2024Quote: ... and were then anesthetized (isoflurane 4 % for induction and 1.5 % for maintenance) before placing in the optical imaging system (IVIS Lumina III, PerkinElmer, Courtaboeuf, France). In vivo bioluminescence signals were measured in selected regions of interest (injected testes ...
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bioRxiv - Cell Biology 2020Quote: ... image stacks (5 × 1 μm optical sections) were acquired on a spinning disc confocal system (Ultraview Vox, Perkin Elmer) with a 60× 1.4 NA oil-immersion objective (Nikon ...
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bioRxiv - Cancer Biology 2022Quote: Optimized numbers of cells (2.5 x 103 to 5 x 103) were plated onto 96-well plates (Perkin Elmer) and allowed to adhere ...
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bioRxiv - Microbiology 2022Quote: ... the dried gel was exposed on phosphoscreen for 5 days and revealed using a Cyclone Plus Phosphor Imager (PerkinElmer).
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bioRxiv - Immunology 2022Quote: 50,000 cells per well were seeded and allowed to attach before gentle washing and incubation for 48 hours in media containing 0.4 µCi/mL (24.24 nM final concentration) [5-3H]glucose (Perkin Elmer) or 2 µCi/mL (66.67 nM final concentration ...
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bioRxiv - Microbiology 2022Quote: ... The membranes were incubated with primary and secondary HRP-conjugated antibodies in PBST (5% milk and 0.1% Tween-20) to detect the protein of interest by Western Lightning Plus ECL (PerkinElmer) and ImageQuant LAS 4000 imaging system (GE) ...
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bioRxiv - Microbiology 2022Quote: ... A series of images spanning 5 µm were acquired with 0.2 µm spacing and images were deconvolved with VOLOCITY software (PerkinElmer) to report a single combined z-stack image.
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bioRxiv - Cancer Biology 2022Quote: ... the reaction was stopped using 5 µL 40 µM EDTA in 1X LANCE Detection buffer (Perkin Elmer, CR97-100) for 5 min ...
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bioRxiv - Immunology 2022Quote: ... 5 μL of the IgG supernatants were transferred to an OptiPlate-384 assay plate (white opaque, PerkinElmer, Waltham, MA), mixed with 10μL (10 nM final conc. ...
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bioRxiv - Immunology 2021Quote: ... Immunohistochemistry was performed in Tris/NaCl blocking buffer (0.1□M Tris-HCl, 0.15□M NaCl, 5□μg/ml blocking reagent (Perkin Elmer), pH 7.5 ...
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Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. colibioRxiv - Microbiology 2020Quote: Autophosphorylation of YegI and point mutants was performed by addition of 5 μCi of [γ-32P] ATP (Perkin Elmer) to 0.2 μM of purified YegI or YegI point mutants in 10 μl of reaction buffer containing 50 mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... with a total length of 109 ntb was prepared by 5′-end-labelling using [γ-32P]- ATP (Perkin Elmer) and T4 polynucleotide kinase (Thermo Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... were collected from the CA1 SR from ≥5-7 sections per slice and processed for iterative deconvolution (99% confidence; Volocity 4.0, PerkinElmer). Three dimensional (3-D ...
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bioRxiv - Biochemistry 2024Quote: ... The dry filter papers were placed in scintillation vials with 5 ml Ultima Gold F scintillation cocktail (Perkin Elmer) and scintillation was measured in a TriCarb 2900TR liquid scintillation analyzer (Perkin Elmer).
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bioRxiv - Genomics 2023Quote: ... The metabolite extract was transferred into scintillation vials with 5 mL Insta-Gel Plus scintillation cocktail (Perkin Elmer #601339).
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bioRxiv - Cancer Biology 2022Quote: ... and maintained in 2.5 mL of KSFM supplemented with 5% (v/v) charcoal-stripped FBS and 50 nM R1881 (PerkinElmer) 25 ...