Labshake search
Citations for PerkinElmer :
651 - 700 of 1358 citations for Recombinant Human High Mobility Group Box 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2023Quote: ... Slides were imaged using Nikon C2 confocal microscope while the 96 wells were imaged by the high content screening microscope Operetta CLS+ Twister (Perkin Elmer). Analysis was performed using Image J and Harmony software (Perkin Elmer) ...
-
bioRxiv - Cell Biology 2023Quote: ... The plate was then washed 5x with PBS and imaged on the Opera Phenix High Content Screening System (#HH14000000, Perkin Elmer). A minimum of nine fields were acquired per well using a 20x water immersion objective in confocal mode ...
-
bioRxiv - Cell Biology 2023Quote: ... The plate was then washed thrice with PBS and imaged on the Opera Phenix High Content Screening System (#HH14000000, Perkin Elmer). A minimum of nine fields were acquired per well using a 20x water immersion objective in confocal mode ...
-
bioRxiv - Neuroscience 2024Quote: RNA-FISH and immunocytochemistry experiments were all imaged using the automated Opera Phenix TM high-throughput confocal imaging platform (Perkin Elmer). Dual RNA-FISH and immunocytochemistry images were analysed using Columbus 2.8 (PerkinElmer) ...
-
bioRxiv - Cell Biology 2023Quote: ... and cells were seeded in 96-well plates under normal growth conditions and fluorescence from tracked live cells was measured using the Operetta High Content Screening System (Perkin Elmer).
-
bioRxiv - Molecular Biology 2024Quote: ... Slides were then imaged on a Nikon C2 confocal microscope while the 96 wells were imaged by the high content screening microscope Operetta CLS+ Twister (Perkin Elmer). Images were analysed using Image J and Harmony softwares (Perkin Elmer ...
-
bioRxiv - Molecular Biology 2024Quote: ... Slides were then imaged on a Nikon C2 confocal microscope while the 96 wells were imaged by the high content screening microscope Operetta CLS+ Twister (Perkin Elmer). Images were analysed using Image J and Harmony softwares (Perkin Elmer ...
-
bioRxiv - Genomics 2019Quote: ... FISH experiments were performed using human fosmids containing non-duplicated region directly labeled by nick-translation with Cy3-dUTP (PerkinElmer), Cy5-dUTP (PerkinElmer) ...
-
bioRxiv - Genomics 2021Quote: Two-color FISH experiments were performed using human fosmid (n=2) or BAC (n=2) clones directly labelled by nick-translation with Cy3-dUTP (PerkinElmer) and fluorescein-dUTP (Enzo ...
-
bioRxiv - Immunology 2020Quote: ... during the final 6 hours (murine MLR) or 15 hours (human MLR) of culture was measured by a TopCount (PerkinElmer).
-
bioRxiv - Bioengineering 2023Quote: ... overexpressing the human FGFR1c together with β-klotho was plated into 384-well micro-titer plates (Perkin Elmer, Cat. #6007480) and incubated at 37°C ...
-
bioRxiv - Neuroscience 2021Quote: Live-cell FRET imaging and analysis was performed as we have previously described (49) using an Opera Phenix™ high content confocal microscopy system (Perkin Elmer). Images were acquired using a 40X water-immersion objective using a 425 nm laser for excitation of CFP ...
-
bioRxiv - Molecular Biology 2020Quote: ... Cells were visualized and % of γ-H2AX-positive cells was calculated using the Operetta high-content imaging and analysis system (Perkin Elmer) with 20X objective.
-
bioRxiv - Systems Biology 2019Quote: ... The insert size of the libraries was verified by running an aliquot of the DNA library on a PerkinElmer GX using the High Sensitivity DNA chip (Perkin Elmer, CLS760672) and the concentration was determined by using a High Sensitivity Qubit assay and q-PCR ...
-
bioRxiv - Genomics 2019Quote: ... Libraries were assessed for concentration and fragment size using the DNA High Sensitivity Assay on the LabChip GX (Perkin Elmer, Waltham, MA). Library concentrations were also assessed by qPCR using the KAPA Library Quantification Kit (Complete ...
-
bioRxiv - Cell Biology 2020Quote: ... The plate slated to be fixed on the last timepoint were imaged by digital phase contrast (Operetta High-Content Imaging System (PerkinElmer, Woodbridge, ON)) every 12 hrs to acquire cell number estimates ...
-
bioRxiv - Cancer Biology 2021Quote: ... the Golgi region of the cells expressing the GT-pHluorin were imaged by using the Operetta™ high content imaging system (PerkinElmer Inc.). In situ pH calibration was done by replacing the bath solution with different pH calibration buffers (125 mM KCl ...
-
bioRxiv - Neuroscience 2021Quote: Live-cell imaging was performed at 37°C and with 3% CO2 using an Opera Phenix™ high content confocal microscopy system (Perkin Elmer). One hour prior to imaging ...
-
bioRxiv - Microbiology 2021Quote: The cytotoxic effect of the wild-type and mutant strains were analyzed by live/dead cell assay using the High Content Screening (HCS) System (Operetta, Perkin Elmer, USA). At 12 h of post-infection ...
-
bioRxiv - Cell Biology 2021Quote: ... The plate slated to be fixed on the last timepoint was imaged by digital phase contrast (Operetta High-Content Imaging System (PerkinElmer, Woodbridge, ON) every 12 hrs to acquire cell number estimates ...
-
bioRxiv - Biochemistry 2022Quote: ... DAPI-stained and imaged and analyzed in a custom workflow on a High Content Screening Operetta CLS apparatus (Perkin Elmer, MA, USA). Alternatively ...
-
bioRxiv - Plant Biology 2022Quote: ... and the eluted DNA quality was assessed with Caliper LabChip GX for DNA samples using the High Sensitivity Assay (PerkinElmer, Inc. USA) and quantified using a Quant-iT dsDNA High Sensitivity Assay Kit on a Qubit fluorometer (Invitrogen ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... quantified with a Fluoroskan instrument and validated using a high-throughput microfluidic capillary electrophoresis LabChip GX system (Perkin Elmer, Waltham, MA, USA).
-
bioRxiv - Bioengineering 2023Quote: ... The optical path in the visible channel also contained a high-speed motorized filter wheel (HS1024, FLI, Lima, NY) and a liquid crystal tunable filter (VariSpect LCTF, PerkinElmer, Waltham, MA) for hyperspectral imaging ...
-
bioRxiv - Biochemistry 2024Quote: ... images were captured from 15 or 5 fields per well and the number of punctate per cell were quantitated using high-content imaging and analysis software (Perkin Elmer; Harmony).
-
bioRxiv - Cell Biology 2024Quote: Primary cilia length in hiPSC-derived neurons stained with DAPI (405 nm) and HaloTag ligand (488 nm) was measured using the Harmony High-Content Imaging and Analysis software (Perkin Elmer, v4.9) using an automated analysis pipeline ...
-
bioRxiv - Developmental Biology 2024Quote: ... Plates (with lid on) were placed in the Opera Phenix High-Content Screening System (Perkin Elmer; prewarmed to 37C and 5% CO2). Images were collected every 30 min for 24 hours.
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... tumors (ca 120,000 cells) consisting of H460 human NSCLC transfected with luciferase and the tdTomato gene (H460 Luc+ tdTomato+, Perkin Elmer France) were injected ectopically into the left flank of 90 mice ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: Radioligand binding assays were performed with membranes prepared from cells expressing human CB2 or CB1 or mouse CB2 receptor using [3H]-CP55940 (Perkin Elmer) as a radioligand ...
-
bioRxiv - Microbiology 2023Quote: Apical wash fractions collected pre- and post-infection were used to detect Lactate dehydrogenase activity and IL-8 concentrations using human LDH and IL-8 Alphalisa Kits (Perkin Elmer). ELISA Kits for the detection of human TNFα ...
-
bioRxiv - Immunology 2020Quote: ... The length distribution of the cDNA libraries was monitored using DNA High Sensitivity Reagent Kit on the Perkin Elmer Labchip GX system (PerkinElmer, Waltham, MA, USA). The libraries were qPCR quantified (Kapa Biosystems ...
-
bioRxiv - Molecular Biology 2020Quote: ... The effects of the compounds were evaluated 24 h later using an Operetta CLS Confocal High Content Imaging System (PerkinElmer, Waltham, Massachusetts, USA) with the following parameters ...
-
bioRxiv - Cell Biology 2019Quote: ... a Zeiss Axiovert 200M inverted fluorescence microscope with a Hamamatsu ORC-AG high-resolution camera and Volocity imaging software (PerkinElmer, Waltham, MA, USA) was used to capture flow of Sphero™ 2 μm high intensity fluorescent beads (Spherotech ...
-
bioRxiv - Immunology 2020Quote: Lymphoid follicles were enumerated in high-powered entire reconstitutions of vaginal sections acquired with the Lamina™ Multilabel Slide Scanner (PerkinElmer, Courtaboeuf, France), manually counting 8 to 14 sections per macaque ...
-
bioRxiv - Biochemistry 2019Quote: ... and the percent of infected cells was assessed by an immunofluorescence assay using Operetta High-Content Imaging System (PerkinElmer, Inc., Waltham, MA, USA).
-
bioRxiv - Microbiology 2023Quote: ... Libraries were assessed for concentration and fragment size using the DNA High Sensitivity Assay on the LabChip GX Touch (Perkin Elmer, Waltham, MA). The library concentrations were also assessed by qPCR using the KAPA Library Quantification Kit (Complete ...
-
bioRxiv - Cancer Biology 2023Quote: ... culture plates were incubated for 3 days after which they were imaged with an Operetta CLS high-content analysis system (Perkin Elmer, LIVE configuration) with the incubation chamber set to 37 °C and 5% CO2 ...
-
bioRxiv - Cell Biology 2023Quote: ... the cell plates were immediately started for live cell imaging on an automated spinning-disk confocal high-content imaging system (Perkin Elmer, Opera Phenix) using a 40X water immersion objective lens under 5% CO2 and 37 °C condition ...
-
bioRxiv - Microbiology 2020Quote: ... 1 µCi of [1-14C]-palmitate (2.22 GBq mmol-1, Perkin Elmer) was added to 10 ml culture medium of exponentially or stationary phase-grown bacteria and further incubated for 1.5 h at 30°C ...
-
bioRxiv - Systems Biology 2023Quote: ... The NPCs were cultured in a 12-well dish to confluency in NPC media and then dissociated using Accutase for 5 minutes at 37°C to single cell resolution and plated on 5 µg/mL human laminin and 5µg/mL fibronectin-coated 384 well plates (Perkin Elmer Cat #6057308) at 12,000 cells/well in NPC media and 10 µM ROCKi ...
-
bioRxiv - Immunology 2024Quote: ... Five-micrometer serial sections were stained with a human-specific anti-COXIV antibody and scanned using the Vectra® Polaris Automated Imaging System (PerkinElmer, USA) at 20×magnification ...
-
bioRxiv - Microbiology 2020Quote: ... signals detected in four spots per well from three independent samples was quantified using the Operetta high content screening system (Perkin Elmer, Waltham, MA, USA) and the built-in Harmony software.
-
bioRxiv - Microbiology 2019Quote: ... The length distribution of the cDNA libraries was monitored using a DNA High Sensitivity Reagent Kit on the Perkin Elmer Labchip (Perkin Elmer, Waltham, MA, USA). All samples were subjected to an indexed paired-end sequencing run of 2×51 cycles on an Illumina HiSeq 2000 system (Illumina ...
-
bioRxiv - Microbiology 2020Quote: ... Nuclei were counterstained with DAPI and after washing the cells were imaged by the Operetta High Content Imaging System (Perkin Elmer Woodbridge, Ontario, Canada), using a 20x magnification objective ...
-
bioRxiv - Bioengineering 2023Quote: ... for SARS-CoV-2 S protein neutralization antibody titer in the wells was observed using the High Content Analysis System (Perkin Elmer, Waltham, MA, USA) or microscope ...
-
bioRxiv - Genomics 2022Quote: SARS-CoV-2 genome libraries were constructed by using the NEXTFLEX® Variant-Seq™ SARS-CoV-2 kit with the automated high-throughput liquid handler Sciclone® G3 NGS (Perkin Elmer, USA) according to the manufacturer’s instructions.
-
bioRxiv - Bioengineering 2022Quote: ... before their observation and scanned using a high-resolution X-ray micro-CT system (Quantum FX Caliper, Life Sciences, Perkin Elmer, Waltham, MA, United States) hosted by the PIV Platform (UR2496 ...
-
bioRxiv - Cell Biology 2021Quote: ... hand-picked human islets were dispersed in accutase for 10 min at 37°C and plated in 96-well plates (Perkin Elmer Inc., Waltham, MA) treated with Poly-D-Lysine Hydrobromide (Sigma-Aldrich ...
-
bioRxiv - Biochemistry 2021Quote: ... an optimized set of 4 small interfering RNAs (siRNAs; SMARTPool) targeted against human Furin were purchased from Horizon Discoveries (Perkin Elmer, Lafayette, LA, USA) and transfections of HeLa cells were carried out using INTERFERin (PolyPlus ...
-
bioRxiv - Molecular Biology 2020Quote: 1 μl ml-1 of 0.1 mCi ml-1 14C glucose (PerkinElmer, Cat# NEC042V250UC, Waltham, MA, USA) was added to differentiation medium 48 h before the end of the experiments ...