Labshake search
Citations for PerkinElmer :
651 - 700 of 1203 citations for Nitrite Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Plates were incubated for 48 h and the degree of viral entry was determined by luminescence using the neolite reporter gene assay system (PerkinElmer). IC50 values were determined by fitting dose-response curves with four-parameter logistic regression in GraphPad Prism 8 software.
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bioRxiv - Microbiology 2021Quote: ... coli TMPK catalysis was measured in the direction of ATP synthesis using the ATPlite luminescence assay (PerkinElmer cat. no. #6016739) in white 384-well plates (Corning cat ...
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bioRxiv - Microbiology 2019Quote: ... were infected with viral particles of Makona GP-A82 or V82 pseudoviruses in a 48 well plate using the same amount of p24 antigen quantified by AlphaLISA (AL291C:500 assay; PerkinElmer). After 48 h of incubation cells were washed twice with PBS and lysed for luciferase assay ...
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bioRxiv - Cell Biology 2019Quote: GBM2 cells were plated at 4000 cells/well and exposed to epigenetic compounds (Table 2) at 10 µM for 1 day in 384-well optical bottom assay plates (PerkinElmer). Negative control was DMSO (0.1%) ...
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bioRxiv - Cell Biology 2019Quote: GBM2 cells were plated at 2000 cells/well and exposed to Prestwick compounds (3 µM; Table 6) for 3 days in 384-well optical bottom assay plates (PerkinElmer). Cells were then fixed and stained with rabbit polyclonal anti-H3K27ac and mouse monoclonal anti-H3K27me3 antibodies followed by AlexaFluor-488- or AlexaFluor-555-conjugated secondary antibodies ...
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bioRxiv - Molecular Biology 2020Quote: ... The signals of time-resolved fluorescence resonance energy transfer (TR-FRET) assay were measured in an Envision plate reader (PerkinElmer) with a TR-FRET mode (Ex=320 ...
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bioRxiv - Biochemistry 2020Quote: ... was incubated with 5 μL of biotinylated peptides at 100 nM for 30 min at room temperature in the epigenetics assay buffer (PerkinElmer AL008 ...
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bioRxiv - Physiology 2020Quote: ... as per manufacturer’s instructions and loaded onto the DNA 5K/RNA/Charge Variant Assay LabChip (Perkin Elmer, Cat. No. 760435) to be read using a LabChip GX Touch Nucleic Acid Analyser (Perkin Elmer ...
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bioRxiv - Immunology 2020Quote: ... Plates were incubated for 48 hours and levels of viral infection were determined by luminescence using the neolite reporter gene assay system (PerkinElmer). Virus alone was used as a control and data was normalized to the control.
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bioRxiv - Microbiology 2021Quote: ... duplicate twofold serial dilutions of various compounds were prepared in assay medium in sterile 96-well black opaque plates (CulturPlate-96 Black, PerkinElmer). To allow for synergy testing ...
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bioRxiv - Immunology 2022Quote: ... using the RNA Assay and DNA 5K/RNA/CZE HT Chip on a LabChip GX Touch HT Nucleic Acid Analyzer (PerkinElmer). TruSeq Stranded mRNA Library Prep kit (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... 48 hpi liver-stage cultures were washed with PBS and lysed in Nano-Glo Luciferase Assay buffer containing Nano-Glo Luciferase Assay substrate diluted 1:200 and luminescence was measured after 3 minutes on an IVIS Specturum CT (PerkinElmer). To monitor merosome detachment at 65 hpi ...
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bioRxiv - Microbiology 2023Quote: ... The tissue lysates were mixed with an equal volume of Nano-GLO Luciferase Assay Reagent (Nanolight) and luciferase activity was quantified on an Envision 2103 plate reader (PerkinElmer). Decoy concentration and half-life were determined based on a standard curve derived from the mixture of serial diluted ACE2.1mb-nLuc proteins and tissue lysate from wild-type ...
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bioRxiv - Neuroscience 2022Quote: ... then incubated for 30 min at 25 °C with shaking at 400 RPM with 9 ng/well of DELFIA Eu-N1 anti-mouse antibody in DELFIA assay buffer (PerkinElmer), washed with 3 × 100 μl of PBST before enhancing with 80 μl of DELFIA Enhancement Solution (PerkinElmer) ...
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bioRxiv - Immunology 2022Quote: ... and interleukin 10 (IL-10) were quantified in the supernatant from the PBMCs using an AlphaLISA assay (PerkinElmer, Waltham, MA). AlphaPlates containing 2 µL of supernatant were thawed at 25°C for 15 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... The average fragment size of the final libraries was determined using DNA 1K/12K/Hi Sensitivity Assay LabChip (760517, PerkinElmer)) and DNA High Sensitivity Reagent Kit (CLS760672 ...
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bioRxiv - Biochemistry 2023Quote: ... The assay was performed according to the manufacturer’s instructions and experiments described in (26) in white-opaque OptiPlate™-384 (PerkinElmer). Experiments to determine Km values were performed with increasing substrate concentrations ...
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bioRxiv - Biochemistry 2023Quote: ... Reactions were initiated by adding an equal volume of assay buffer containing 1 μM [35S]GTPγS (~50 cpm/fmol, Perkin Elmer) at 30 °C ...
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bioRxiv - Immunology 2023Quote: ... After 10min of incubation at 37°C supernatants were collected and concentration of ATP/sample was measured using ATPlite Luminescence Assay System (PerkinElmer) according to the manufacturer’s protocol.
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bioRxiv - Bioengineering 2023Quote: ... RNA integrity was analyzed using automated capillary electrophoresis (RNA Pico Sensitivity Assay, LabChip GX II Touch HT instrument, Perkin Elmer). Samples were stored at –80°C until further analysis.
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bioRxiv - Cell Biology 2020Quote: ... Lysis and luciferase activity detection was performed using the Gaussia-Juice Luciferase Assay (PJK Biotech, Kleinblittersdorf, Germany) according to the manufacturer’s description in white opaque 96-well plates (OptiPlate, PerkinElmer, Waltham, MA) and using a Synergy Mx microplate reader (BioTek ...
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bioRxiv - Bioengineering 2022Quote: Saturation assays for binding of K1K1H6 to recombinant MET-Fc protein were performed in 384-well microtiter plates (OptiPlate™-384, PerkinElmer© ...
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bioRxiv - Neuroscience 2021Quote: ... 100 µL of 2% ACN/0.1% TFA was added to the reaction mixture and the mixture was analyzed by mobility shift assay (LabChip EZ Reader II; PerkinElmer, U.S.A.). The kinase activity is the percentage of phosphorylated peptide signal over the total substrate peptide signal ...
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bioRxiv - Biophysics 2021Quote: ... Validation assays for 5-HT5AR-Gi1 expression were performed as described above using 10nM [3H]-LSD (82.4 Ci/mmol, PerkinElmer, Waltham, MA).
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bioRxiv - Cell Biology 2022Quote: ... An equal volume of cell culture media and esterase substrate (100 μM in esterase assay media at pH 5) was transferred to a black walled clear bottomed plate (Perkin Elmer) and fluorescence was measured after 1 h.
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bioRxiv - Biochemistry 2019Quote: ... The BMAL TAD probe was diluted to a working concentration of 50 nM in assay buffer and binding was monitored by changes in fluorescence polarization with an EnVision 2103 multilabel plate reader (Perkin Elmer) with excitation at 531 nm and emission at 595 nm ...
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bioRxiv - Biophysics 2019Quote: AlphaScreen-based peptide recruitments assays were performed using streptavidin coated donor beads and anti-His-tag antibody coated AlphaLisa acceptor beads (Perkin Elmer). The assays were very sensitive to the distance of the N-terminus to the LXXLL NR-box motif and the use of a flexible Ahx linker ...
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bioRxiv - Physiology 2020Quote: ... The total concentration and purity of resulting RNA was determined using the RNA Pico Sensitivity Assay (Perkin Elmer, Cat. No. CLS960012) as per manufacturer’s instructions and loaded onto the DNA 5K/RNA/Charge Variant Assay LabChip (Perkin Elmer ...
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bioRxiv - Biochemistry 2022Quote: Michaelis-Menten kinetics of the untagged purified MtCM variants were determined by a continuous spectroscopic chorismate depletion assay (Lambda 20 UV/VIS spectrophotometer, Perkin Elmer). The enzymes were diluted to working stock solutions in 20 mM potassium phosphate ...
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bioRxiv - Cancer Biology 2023Quote: ... Firefly and Renilla luciferase activities were measured using the Twinlite dual luciferase reporter gene assay system (Perkin Elmer, Wellesley, MA, USA) on an automated TECAN luminometer (TECAN Group Ltd ...
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bioRxiv - Microbiology 2022Quote: ... and serum IgA antibodies to the secreted form of S-2P were measured by europium ion-enhanced DELFIA-TRF assay (Perkin Elmer) following the supplier’s protocol.
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bioRxiv - Immunology 2022Quote: ... the medium was removed from all wells and cells were lysed by the addition of 100 µl of a 1:1 dilution of BriteLite assay readout solution (Perkin Elmer) and 1xDPBS (Gibco ...
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bioRxiv - Synthetic Biology 2023Quote: ... the Her2 VHH library was resuspended in 500 µL Triton X-100 based stain buffer (AlphaLISA immunoassay assay buffer from Perkin Elmer; 25 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: MLL1C binding assays (Figure 2C) were performed as above except using Nickel-chelate acceptor beads (10 μg/mL; Perkin Elmer AL108M), streptavidin donor beads (20 μg/mL ...
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bioRxiv - Biochemistry 2023Quote: ... half the medium was removed from all wells and cells were lysed by the addition of 50 µl of BriteLite assay readout solution (Perkin Elmer) to make a a 1:1 dilution into each well ...
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bioRxiv - Immunology 2023Quote: ... Libraries were assessed for concentration and fragment size using the DNA High Sensitivity Assay on the LabChip GX Touch (Perkin Elmer). The resulting libraries were sequenced on a multiplexed paired end 100bp Illumina NovaSeq 6000 S4 flowcell ...
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bioRxiv - Neuroscience 2023Quote: ... Horseradish peroxidase activity was visualized using an enhanced chemiluminescence assay in a Western Lightning Plus system (Perkin Elmer, Waltham, MA, USA). Chemiluminescent imaging was performed on a FUSION FX system (Vilber ...
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bioRxiv - Microbiology 2023Quote: ... filtered through a 0.45-µm-pore size filter and quantified for viral Cap24 content using enzyme-linked immunosorbent assay (ELISA) (Perkin Elmer, # NEK050A) as previously published (41 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Plasma Lithium was measured using Inductively Coupled Plasma Mass Spectrometry assay (NexION 2000™ spectrometer, Perkin Elmer, Villebon-sur-Yvette, France). At the end of the protocol (6 months) ...
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bioRxiv - Systems Biology 2021Quote: ... Imaging was performed by HRP luminescence using Femto kit (ThermoFischer Scientific) or Western Lightning PlusECL kit (Perkin Elmer).
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bioRxiv - Neuroscience 2020Quote: ... and TSA Plus Fluorescein amplification kit (PerkinElmer) for 1 hour and 10 min ...
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bioRxiv - Neuroscience 2022Quote: ... amplified using TSA Plus Biotin kit (PerkinElmer) and visualized with Alexa Fluor 488-conjugated streptavidin (1:200 ...
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bioRxiv - Neuroscience 2022Quote: ... amplified using TSA Plus DIG kit (PerkinElmer) and visualized with DyLight 594-conjugated anti-DIG antibody (1:500 ...
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bioRxiv - Cancer Biology 2020Quote: The Lance Ultra cAMP kit (Perkin Elmer) was used to measure the effect of unacylated ghrelin on cAMP production ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated with tyramide (TSA kit, PerkinElmer) for 10 min ...
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bioRxiv - Molecular Biology 2020Quote: ... Enhanced Chemiluminescence Substrate kit (PerkinElmer ref. NEL103E001EA). The percentage of m6A in the main “band” co-migrating with the 2 kb molecular weight standard was measured by the intensity of the “band” divided by the signal intensity of the whole lane.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the LANCE Ultra cAMP kit (Perkin Elmer) was used according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... TSA Plus Fluorescence Kit (Perkin Elmer, # NEL741B001KT) was used for signal amplification ...
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bioRxiv - Cell Biology 2023Quote: ... the cAMP-Gi kit (Perkin Elmer, TRF0263) was used ...
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bioRxiv - Immunology 2023Quote: ... using the ATPLite kit (6016943; Perkin Elmer), according to the manufacturer’s instructions.