Labshake search
Citations for PerkinElmer :
651 - 700 of 817 citations for Human Procollagen Type III N Terminal Propeptide PIIINP ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Probe binding was indicated by deposition of target-specific fluorophores at each location via TSA Plus Fluorescence kit (PerkinElmer), and sections were then counterstained with DAPI ...
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bioRxiv - Physiology 2021Quote: ... acylcarnitines were derivatized to their butyl esters and treated with the reagents of the NeoGram MSMS-AAAC kit (PerkinElmer). Their analysis was carried out on a Waters 2795/Quattro Micro AP liquid chromatography–tandem mass spectrometer (Waters ...
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bioRxiv - Neuroscience 2021Quote: ... Strand-specific libraries with unique barcodes were prepared using the NEXTFLEX Rapid Directional RNA-Seq kit 2.0 according to manufacturer’s instructions (Perkin Elmer). Libraries were pooled in equal molar amounts and sequenced on an Illumina HiSeq 2500 to obtain roughly 40 million reads per sample.
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bioRxiv - Microbiology 2021Quote: ... the resulting viral particles were collected and titrated in VeroE6 cells by enzyme luminescence assay (Britelite plus kit; PerkinElmer), as described previously [91].
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bioRxiv - Genetics 2022Quote: ... Amplified products were quantified with Quant-iT(tm) PicoGreen dsDNA Assay Kit on a VICTOR Multilabel Plate Reader (PerkinElmer) and pooled in equimolar ratios ...
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bioRxiv - Immunology 2022Quote: ... and the resulting viral particles were titrated in TZMbl cells using an enzyme luminescence assay (britelite plus kit; PerkinElmer) as described previously [82] ...
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bioRxiv - Microbiology 2021Quote: ... Pooled samples were size selected with the LabChip XT (LabChip XT DNA 300 Assay Kit; PerkinElmer, Seer Green, UK) and diluted to 26 pM for templating with the Ion OneTouch 2 system using the Ion PGM Template OT2 200 kit ...
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bioRxiv - Physiology 2020Quote: ... The fluorescence signal was developed with tyramide signal amplification (TSA) Plus Cyanine 3 System kit (PerkinElmer, Waltham, Massachusetts, USA) as described previously (Tulke et al. ...
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bioRxiv - Immunology 2021Quote: ... with dsDNA HS (High Sensitivity) Assay Kit and LabChip GX Touch using a High Sensitivity DNA chip (Perkin Elmer). Libraries were then equimolarly pooled and quantified by qPCR using the KAPA library quantification kit (Roche) ...
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bioRxiv - Microbiology 2021Quote: ... Viral particles were titrated using an HIV-1 p24Gag Alpha-Lisa kit and an Envision plate reader (Perkin Elmer) and/or by determining their infection titers on target cells.
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bioRxiv - Microbiology 2021Quote: The quantification of HIV-1 p24 capsid protein was performed in captured virus lysates and virus-containing supernatants with the high-sensitivity AlphaLISA p24 detection kit following the manufacturer’s (PerkinElmer) instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Luciferase activity was measured using Pierce Firefly Luciferase Glow Assay Kit according to manufacturer’s protocol using an EnVision multilabel plate reader (PerkinElmer). AlamarBlue (ThermoFisher ...
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bioRxiv - Microbiology 2022Quote: ... The above extracted microbial DNA was then subjected to amplification and sequencing of the V4 region of the 16S rRNA gene by using a NEXTflex 16S V4 Amplicon Seq kit 2.0 (PerkinElmer), and sequences were generated on the Illumina MiSeq platform (Illumina) ...
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bioRxiv - Microbiology 2022Quote: ... Viral particles were titrated using an HIV-1 p24Gag Alpha-Lisa kit and an Envision plate reader (Perkin Elmer). VSV-G pseudotyped HIV-1 (NL4.3 ...
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bioRxiv - Molecular Biology 2024Quote: ... mRNA was isolated from 150ng total RNA using a Nextflex Poly-A Selection kit (Perkin Elmer, Waltham, MA, USA). cDNA libraries were prepared using a Nextflex Rapid Directional RNA 2.0 kit ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Samples were processed according to the NEXTFLEX® Small RNA-Seq Kit v4 with UDIs (PerkinElmer, #NOVA-5132-32) with a 1 µg starting input and 12 cycles of PCR ...
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bioRxiv - Pathology 2023Quote: ... Supernatant was discarded and DNA was extracted using the QuicPickTM SML Plant DNA purification kit according to the supplier’s instructions (Bio-Nobile, Turku, Finland) in an automated system (Caliper Zephyr, PerkinElmer). DNA was eluted from magnetic beads using 30 µL of elution buffer ...
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bioRxiv - Genomics 2023Quote: ... the converted DNA was then used for the library preparation using the NEXTFLEX® Bisulfite-Seq kit (Perkin Elmer) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA from standard cultures were prepared according to manufacturer’s protocol using the NextFlex Combo-Seq mRNA/miRNA kit (PerkinElmer), to capture both the mRNA and miRNA in the samples ...
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bioRxiv - Cell Biology 2023Quote: ... with dsDNA HS (High Sensitivity) Assay Kit and LabChip GX Touch using a High Sensitivity DNA chip (Perkin Elmer). Libraries were then equimolarly pooled and quantified by qPCR using the KAPA library quantification kit (Roche) ...
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bioRxiv - Physiology 2023Quote: ... and its integrity was assessed using 5K / RNA / Charge Variant Assay LabChip and RNA Assay Reagent Kit (Perkin Elmer). Libraries were generated from 250 ng of total RNA as following ...
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bioRxiv - Immunology 2023Quote: RNA libraries were generated using the NEXTFLEX® Small RNA-Seq Kit (PerkinElmer, USA; Cat. No.: NOVA-5132-05). For each library ...
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bioRxiv - Microbiology 2024Quote: ... DNA extraction was performed using the Chemagic™ Prime Viral DNA/RNA 300 Kit H96 (Chemagic, CMG-1433, PerkinElmer). Detailed concentration and DNA extraction procedures are provided in the Supplementary materials (Materials and Methods ...
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bioRxiv - Immunology 2024Quote: ... with dsDNA HS (High Sensitivity) Assay Kit and LabChip GX Touch using a High Sensitivity DNA chip (Perkin Elmer). Libraries were then equimolarly pooled and quantified by qPCR using the KAPA library quantification kit (Roche) ...
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bioRxiv - Molecular Biology 2024Quote: PDE8A activity assay was performed with recombinant PDE8A and cAMP detection using the LANCE Ultra cAMP kit (Perkin Elmer). To determine the most sensitive range for the assay ...
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bioRxiv - Molecular Biology 2024Quote: ... The concentration of this first messenger was determined using the Lance Ultra cAMP kit (Perkin Elmer, Waltham, MA, US). The method consists of a time-resolved fluorescence resonance energy transfer (TR-FRET ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by library preparation with the Next-Flex small RNA v.4 kit protocol (Perkin Elmer, NOVA-5132-06), in accordance to manufacture recommendations ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... IP1 levels were determined using the HTRF® IP-one assay kit as per manufacturer’s instructions and fluorescence measured at 615 and 665 nm using the Envision plate reader (PerkinElmer) after excitation at 340 nm ...
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bioRxiv - Evolutionary Biology 2024Quote: ... An additional library was prepared in-house for the wild male with the NextFlex Rapid DNA-seq 2.0 kit (PerkinElmer), using half the recommended reaction volume ...
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bioRxiv - Genomics 2024Quote: DNA was extracted from whole blood using the Chemagic™ DNA Blood 400 H96 kit (Perkin Elmer, CMG-1091). SNP genotyping was conducted using the Axiom™ Precision Medicine Research Array from Applied Biosystems (Applied Biosystems ...
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bioRxiv - Bioengineering 2020Quote: ... Genomic DNA was isolated from a single colony using a JANUS Chemagic Workstation and Chemagic Viral DNA/RNA kit (PerkinElmer). Libraries were prepared with the Nextera XT DNA sample preparation kit (Illumina) ...
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bioRxiv - Molecular Biology 2020Quote: ... Luciferase activity was measured using Pierce™ Firefly Luciferase Glow Assay Kit according to manufacturer’s protocol using an EnVision mulitlabel plate reader (PerkinElmer). AlamarBlue (ThermoFisher ...
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bioRxiv - Molecular Biology 2021Quote: ... Small RNA libraries were prepared from 1 ng of RNA using the NEXTflex Small RNA-seq Kit v3 (Perkin Elmer). The size distribution of the libraries was assessed on a Bioanalyzer with a DNA High Sensitivity kit (Agilent Technologies) ...
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bioRxiv - Genomics 2022Quote: ... The DNA extraction was performed at Hospital Universitari Vall d’Hebron (Supplementary Table 2) from blood samples using chemagic DNA Blood400 Kit LH (Perkin Elmer). The DNA from oral mucosa swabs ...
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bioRxiv - Genetics 2021Quote: ... 100 ng of polyA-selected mRNA from each meiotic stage was prepared using the NEXTFLEXTM Rapid Directional RNA-seq Kit (NOVA-5138-08; PerkinElmer) according to the provided manual ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 75] and Illumina sequencing libraries prepared using PerkinElmer NEXTFLEX Rapid Directional RNA-Seq Kit 2.0 (Perkin Elmer, Waltham, MA, USA).The libraries were sequenced using a S4 NovaSeq flow cell with the MiSeq 600-cycle v3 kit ...
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bioRxiv - Microbiology 2021Quote: ... The libraries were quantified on the Perkin Elmer LabChip GX Touch with the DNA High Sensitivity Reagent kit (Perkin Elmer). Libraries were pooled in equimolar ratios ...
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bioRxiv - Neuroscience 2020Quote: ... Extracellular culture media were collected in order to dose secreted Aβ using Alpha-LISA assays (AlphaLISA Amyloid β1-X Kit, AL288C, PerkinElmer) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... Prior to sequencing libraries were prepared using NEXTFLEX Rapid DNA-Seq Library Prep Kit for Illumina Platforms (PerkinElmer, Waltham, MA) according to manufacturer’s protocol ...
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: Phosphorylated SYK (p-Syk) was measured using the AlphaLISA SureFire Ultra p-Syk Assay Kit (PerkinElmer, #ALSU-PSYK-A-HV) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Plasmid DNA was sequenced on both strands by primer walking using the ABI PRISMDye Terminator Cycle Sequencing Ready Reaction kit (PerkinElmer) and an ABI PRISM 377 sequencer (Perkin-Elmer ...
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bioRxiv - Systems Biology 2020Quote: ... the DIG-labeled probe was visualized using a tyramide-Cy3 Plus kit (1:50 dilution, 15-minute incubation, Perkin Elmer). Following washes in phosphate buffered saline (PBS ...
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bioRxiv - Neuroscience 2020Quote: Phospho-Syk level from WT mouse primary microglia cultures were determined using AlphaLISA SureFire Ultra kit (PerkinElmer, Waltham, MA, USA) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Detection was performed using the either the TSA Cyanine 3 Plus or the TSA Plus Fluorescein Evaluation Kits (Perkin Elmer).
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bioRxiv - Microbiology 2020Quote: ... The membrane was washed in PBS containing 0.1% Tween-20 and then exposed to developing solution (Western Lighting Chemiluminescence kit, Perkin Elmer) according to manufacturer’s guidelines ...
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bioRxiv - Evolutionary Biology 2020Quote: ... For cDNA generation and construction of the sequencing libraries we used the NEXTflex Rapid Illumina Directional RNA-Seq Library Prep Kit (PerkinElmer) followed by paired-end sequencing on an Illumina NextSeq 500 at Viroscan3D (Lyon ...
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bioRxiv - Cell Biology 2021Quote: ... Luciferase activity was measured using a Promega Nano-Glo Assay Kit and read on an Envision microplate luminometer (Perkin Elmer).
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bioRxiv - Physiology 2020Quote: ... The probes were then detected using Tyramide signal amplification (TSA) reaction using an Alexa-flour labelled TSA kit (PerkinElmer Bioscience) based on the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: HDL-sRNA libraries were generated using the NEXTFlex Small RNA-Seq Kit v3 with 1:4 adapter dilution and 22 PCR cycles (PerkinElmer). Following PCR amplification ...
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bioRxiv - Neuroscience 2021Quote: ... The DNA extraction from K2-EDTA-blood tubes was performed with chemagic 360 instrument and the CMG-704 kit (PerkinElmer), which uses magnetic bead technology ...