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Citations for PerkinElmer :
601 - 650 of 651 citations for Monocyclohexyl Phthalate Unlabeled 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Dissociated cells were resuspended in 7.5 mg/mL BME-2 matrix gel at 2-5×104 cells/mL and seeded in 10 μL droplets to individual wells of 96-well Cell Carrier Ultra plates (Perkin Elmer). Once settled ...
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bioRxiv - Cancer Biology 2023Quote: ... Mice were anesthetized at 37 °C with inhaled isoflurane in an induction chamber for 5 min and received an intraperitoneal injection of luciferin (150 mL, RediJect D-Luciferin Ultra Bioluminescent Substrate, PerkinElmer, 770505). Bioluminescence images were taken 13 minutes after injection ...
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bioRxiv - Systems Biology 2023Quote: ... 0.5 mg/ml was added 1-hour prior to medium collection and fluorescence intensities were analyzed with plate reader (PerkinElmer 2030).
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bioRxiv - Biochemistry 2023Quote: ... 690 uL of the samples were transferred to a scintillation vial with 2 mL of Insta-Gel Plus scintillation cocktail (Perkin Elmer). Radioactivity was measured with the Scintillation counter (Perkin Elmer) ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were pulsed with 0.1 mCi/ml 35S-L-methionine and 35S-L-cysteine mix (EasyTag™ EXPRESS35S Protein Labeling Mix, Perkin Elmer) in methionine- and cysteine-free DMEM for 15 min ...
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bioRxiv - Microbiology 2024Quote: ... 2×106 bacteria/mL in PBS were seeded in 384-well black clear-bottom plate (ViewPlate®-384 F TC, PerkinElmer) in the presence of 12.5 % BRC ...
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bioRxiv - Molecular Biology 2024Quote: ... and 50 ng was used for random priming reactions in the presence of the radiolabeled nucleotide [α-32P]-dCTP (3,000 Ci/mmol, 10 mCi/ml, Perkin Elmer) using a random primer DNA labeling kit (TaKaRa ...
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bioRxiv - Cancer Biology 2024Quote: ... 150 μL 14C-labelled lipids from the denser organic fraction were combined with 4 mL OptiPhase HiSafe 3 liquid scintillation cocktail (PerkinElmer; 1200.437) and radio-labeling was quantified using a Tri-Carb 2910 TR Liquid Scintillation Analyzer (PerkinElmer) ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 ng of heat-denatured DNA was incubated for 30 min at 37°C in a total volume of 25 μl containing random primer and 5 μl of [α-32P]-dCTP (3,000 Ci/mmol, 10 mCi/ml; Perkin Elmer). After the reaction ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were counterstained with DAPI (1 μg ml-1) before imaging with the Opera Phenix High Content Screening System (Perkin Elmer).
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bioRxiv - Cancer Biology 2024Quote: ... with the luciferase substrate D-luciferin (200µL of 15mg/ml - assuming an average animal weight of 24-26gr) (Perkin Elmer, 122799) and incubated for 10 minutes at room temperature before images were taken using IVIS Spectrum In Vivo Imaging System (Perkin Elmer) ...
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bioRxiv - Neuroscience 2023Quote: ... the biosensor cells were plated at a density of 0.075 x 106 cells/mL in a CellCarrier 96-well plate (PerkinElmer, Cat. # 6005550). As described above ...
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bioRxiv - Neuroscience 2023Quote: Biosensor cells were plated at a density of 0.15 x 106 cells/mL in a CellCarrier 96-well plate (PerkinElmer, Cat. # 6005550). Cells were transfected with 50 nM R2/R3 fibrils premixed in Opti-MEM™ I Reduced Serum Medium supplemented with P3000™ reagent (0.25 µL/well ...
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bioRxiv - Cell Biology 2020Quote: Posttranslational labeling was performed by incubating cells in normal growth medium containing 400 mCi/mL [3H]glutamine (PerkinElmer, 30-60 Ci/mmol) in the presence of (100 µg/ml ...
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bioRxiv - Microbiology 2022Quote: ... we collected 22 mL of gas from each jar and analyzed it using a Clarus 400 gas chromatograph (PerkinElmer Ltd., Beaconsfield, UK) equipped with an automatic split/splitless injector and a flame ionization detector (FID) ...
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bioRxiv - Neuroscience 2019Quote: ... Confluent cultures were changed by quiescent medium for 24 h before they were labelled for 4 h with 1 μCi/ml [3H] arachidonic acid (PerkinElmer Life Sciences). Washing cells once with PBS containing 0.1% free fatty acid albumin and twice with PBS alone ...
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bioRxiv - Cancer Biology 2020Quote: ... proteins were first labeled for 48 h with 0.25 μM Ci ml-1 L-[U-14C] VALINE (Perkin Elmer, Waltham, MA, USA) in GIBCO-RPMI 1640 medium (Invitrogen ...
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bioRxiv - Bioengineering 2020Quote: ... Levels of luciferase production from glioblastoma cells were measured using D-luciferin (150 μg/ml final concentration, Perkin Elmer, Waltham, MA, USA) and luminescence measurement in a plate reader after 15 minutes ...
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bioRxiv - Cell Biology 2021Quote: RAW cells were incubated for 24 h with inositol-free DMEM (MP Biomedica, CA) containing 10 μCi/ml myo-[2-3H(N)] inositol (Perkin Elmer, MA), 1X insulin-transferrin-selenium-ethanolamine (Gibco) ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were fixed with 2% PFA at RT for 30 min and stained with the Hoechst 33342 dye (1 μg/ml) at 37 °C and 5% CO2 before imaging with the Operetta™ high content imaging system (PerkinElmer Inc.). Forty fields were acquired from each well using appropriate filter sets ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293T cells transfected with FTTTRA25T and were labeled for 30 min with EasyTag Express [35S] Protein Labeling Mix (0.1 mCi/mL; Perkin Elmer CAT# NEG772014MC) in DMEM lacking both Cys and Met (Gibco CAT# 21013024 ...
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... at 37°C for 1 h in a reaction containing 20 pmol probe, 1X T4 PNK Reaction Buffer, 50 pmol [γ-32P]-ATP (7000 Ci/mmol, 150 mCi/ml)(PerkinElmer, NEG002Z250UC). For hybridization ...
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bioRxiv - Cell Biology 2021Quote: ... media was removed and replaced with serum-free Modified Eagles Medium (MEM; Mediatech) containing 1 µCi/ml of 3H-myo-inositol (PerkinElmer Life Sciences). Forty-eight hrs post-infection ...
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bioRxiv - Biochemistry 2021Quote: ... metabolic labeling was started by replacing the medium with phosphate-free DMEM containing 10% dialyzed FBS and 1mCi/mL 32P orthophosphate (PerkinElmer, Waltham, MA). After labeling for 8 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... was added to a final concentration of 150 μL/mL and luciferase signal was measured using the Envision® 2105 Multimode Plate Reader (Perkin Elmer). Alternatively ...
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bioRxiv - Neuroscience 2023Quote: ... Half of the cell Triton X lysate from each well was pipetted into a scintillation vial and 4 ml of scintillation fluid (#6013329, Ultima GoldTM, Perkin Elmer, USA) was added ...
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bioRxiv - Molecular Biology 2023Quote: ... GLuc production was measured as a surrogate for IFN-β promoter activation by adding 25 µl coelenterazine-solution (1 µg/ml in H2O) was to 25 µl supernatant.reader (Perkin Elmer, Waltham, USA) and luminescence was measured ...
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bioRxiv - Biochemistry 2024Quote: ... whereafter lysed cells were added to 2 mL scintillation fluid and radioactivity was measured using a scintillation counter (Perkin Elmer 2450 Microbeta2). Data were analyzed using GraphPad Prism 8.0.0 and fitted to a four-parameter logistic curve from where the IC50 and the 95% confidence interval were calculated ...
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bioRxiv - Cancer Biology 2024Quote: ... Mice were injected IP with 10 µL/g of body weight of 15 mg/mL in vivo grade D-Luciferin (luciferin; PerkinElmer, Waltham, MA), anesthetized with 2-3% isoflurane in an induction chamber ...
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bioRxiv - Immunology 2021Quote: ... were plated in 3 ml of complete medium supplemented with IL-2 (20 IU/ml) and incubated with 1 μl of 3H-labeled ROL (PerkinElmer, Boston, MA, USA) for 4 h ...
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bioRxiv - Bioengineering 2021Quote: ... The volume of samples was adjusted to 10 mL by adding ultra-pure water and Gd concentration in each sample was measured using ICP-Mass Spectrometer (PerkinElmer, NexION® 2000). A similar procedure was used for monolayer cell culture (the cells were rinsed with PBS (3x 5 min ...
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bioRxiv - Cancer Biology 2020Quote: ... containing 10% BSA and 3 µl [35S] EasyTag Express protein labelling mix (7 mCi, 259 MBq, 1175 Ci/mmol in 632 ml; Perkin Elmer, Espoo, Finland). After 2 h of incubation at 37 °C cells were washed twice with PBS ...
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bioRxiv - Microbiology 2020Quote: ... at a final MTT concentration of 0.45 mg/ml or by use of a ATPlite Luminescence Assay System (PerkinElmer, Waltham, Massachusetts, United States) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: ... sedated mice were injected with 100 μl (30 mg/ml D-luciferin) in an isoflurane administrating chamber and then placed in an IVIS Lumina III XR machine (Perkin Elmer, Norwalk, CT). IVIS values on day three post tumor implantation were taken to allocate mice into balanced treatment groups of PBS-injected ...
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bioRxiv - Biophysics 2019Quote: ... The solutions were transferred to a 2 mL disposable plastic cuvette and absorbance was measured using a spectrophotometer at a wavelength of 833 nm (Lambda XLS, Perkin Elmer, Hamburg, Germany). The blank value was deducted from the standard solutions and a calibration curve was prepared by plotting absorbance against the amount of phospholipid (micromoles) ...
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bioRxiv - Microbiology 2019Quote: ... At 6 hpi the cells were starved in a glucose-free medium for 30 min followed by labeling with 200 µCi/ml of mannose-D-[2-3H(N)] (Perkin Elmer, Shelton, CT) for 1.5 h ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... After 6-24 h at 37 °C (as specified in each figure legend) the cell medium was aspirated and substituted with serum-free cell medium containing [3H]thymidine (2 μCi/ml, Perkin Elmer, Waltham, MA). The incubation was continued for 30 min at 37 °C ...
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bioRxiv - Pathology 2021Quote: ... primary hepatocytes were serum-starved for 3 hours and incubated for 3-hour with [1-14C] acetate (1 μCi/ml; Perkin Elmer, Boston, MA) and 5.5 mM of non-labeled (cold ...
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bioRxiv - Immunology 2020Quote: ... luciferase expressing target cells were transferred to a white round-bottomed 96-well plate in the presence of Xenolight D-Luciferin (75 μg/mL; Perkin Elmer, Oslo, Norway). CAR-expressing T cells were subsequently added in the mix and cells were cultured in an incubator (37 °C in 5% CO2 ...
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bioRxiv - Genetics 2019Quote: ... evaporated under nitrogen gas (Organomation Associates, Inc., Berlin, MA, USA) and resuspended in 10 mL Insta-Gel Plus scintillation fluid (Perkin Elmer, Cat#6013399), followed by counting with a Beckman LS6500 scintillation counter ...
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bioRxiv - Molecular Biology 2022Quote: U10 and CUGCUGUUUU (20 pmol) were chemically synthesized (Integrated DNA Technologies (IDT)) and 20 pmol RNA was radioactively labeled using [γ-32P]-ATP (PerkinElmer, 10mCi/ml) and 10 units T4 Polynucleotide Kinase (PNK ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were lysed by adding 0.2 mL Optiphase Supermix cocktail and accumulated [3H]DA-radioactivity was measured using a microplate scintillation counter (MicroBeta2 LumiJET, PerkinElmer, Waltham, MA, USA). DAT-specific [3H]DA transport was calculated as the difference in counts obtained in the presence or absence of the DAT-specific antagonist GBR12909 (100 nM) ...
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bioRxiv - Microbiology 2023Quote: ... The filters were washed with 20 ml H2O and the radioactivity retained on the filters was determined by scintillation counting (TriCarb B2810, PerkinElmer, Rodgau-Jügesheim, Germany). The amounts of intracellular phosphate were calculated in nmol*(mg of protein)-1 ...
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bioRxiv - Neuroscience 2021Quote: ... and loaded overnight with MEM +HIHS cell culture media supplemented with 4 μCi/mL D-[2,3-3H]aspartate (Perkin Elmer, Waltham, MA, cat. #NET501001MC). Coverslips were washed in isosmotic Basal medium containing (in mM ...
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bioRxiv - Plant Biology 2020Quote: ... 5 to 10 mg powder were mixed with 2 ml scintillation cocktail and counts per minute (cpm) recorded with a TRI-Carb 2810TR liquid scintillation analyzer (Perkin Elmer, Waltham, MA, USA).
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bioRxiv - Microbiology 2019Quote: ... Cells were resuspended in 0.1 mL PBS and the assay started by the addition of 0.1 mL of the transport mixture containing [3H]-proline (PerkinElmer’s NEN® Radiochemicals; 0.4 μCi) in the presence of different concentrations of the indicated drug ...
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bioRxiv - Microbiology 2019Quote: ... the cells were starved for 30 min in the methionine-free medium before pulse-labeling with 25 µCi/ml of Easy Tag Express 35S labeling mix (Perkin Elmer, Shelton, CT, USA) for 30 min ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The cells were lysed with NaOH (1 M, 1 mL) and transferred to RIA tubes for counting in a γ-counter (Perkin Elmer, Wallac Wizard 1480). The uptake and internalized fraction of the radiotracers were expressed as the percentage of total added activity to each well.
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bioRxiv - Microbiology 2021Quote: ... BIOTECHNOLOGIES Ltd) before counting on a HINDEX 300SL in 5 ml of scintillation fluid (Scintillation fluid, ULTIMA GOLD™, Perkin Elmer, Inc. LOT No.: 77-18151).
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bioRxiv - Cell Biology 2021Quote: ... were then added to the COS-7 cells in the presence of 50 μg/ml PI and imaged immediately at 37°C with 5% CO2 using a spinning disk confocal microscope (UltraVIEW VoX, PerkinElmer with a 40X 1.3 N.A. oil objective). Images were acquired at 1 frame/20s and analyzed using NIH ImageJ software ...