Labshake search
Citations for PerkinElmer :
601 - 650 of 798 citations for 2 4 Methyl 5 thiazolyl ethyl decanoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... Proteins were labelled for 5 mins (for soluble proteins) or 15 mins (for membrane proteins) with 30 µCi of Express Protein Labelling Mix (Perkin Elmer) per sample at their restrictive temperature ...
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bioRxiv - Microbiology 2023Quote: ... collected water samples (50 mL) were immediately spiked with 5 µCi of NaH14CO3 (Perkin Elmer, specific activity 56 mCi mmol−1). The samples were incubated for 24 h under in situ natural illumination ...
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bioRxiv - Molecular Biology 2023Quote: Glassware and plasticware used for RNA gel electrophoresis were soaked in 10% bleach for 5 minutes before washed by COUNT-OFF™ Surface Cleaner (PerkinElmer) and liberally rinsing in double-distilled water ...
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bioRxiv - Immunology 2023Quote: ... Iodine-125 (125I) labeling was performed by incubating DTPA-bsAbs in iodogen coated Eppendorf tubes with Na125I (10−5 M NaOH, Perkin Elmer) in 0.5 M phosphate buffer (pH 7.4 ...
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bioRxiv - Pathology 2023Quote: ... as previously described [71] using Multiplex Fluorescent Reagent Kit V2 (Advanced cell Diagnostics) and TSA Plus Cyanine 3 and Cyanine 5 (Perkin Elmer) as fluorophores according to manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... then each well was incubated for 30 min at 37 °C with 5 μl/well EXPRE35S35S Protein Labeling Mix (PerkinElmer NEG072002MC), after which cells were lysed in RIPA lysis buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Immunology 2023Quote: ... TZM-bl target cells were seeded at a density of 5 × 103 cells/well in 96-well luminometer-compatible tissue culture plates (Perkin Elmer) 24 h before infection ...
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bioRxiv - Systems Biology 2023Quote: ... 0.5 mg/ml was added 1-hour prior to medium collection and fluorescence intensities were analyzed with plate reader (PerkinElmer 2030).
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bioRxiv - Cell Biology 2023Quote: ... 1 mL fractions containing soluble inositol phosphates were mixed with 3 mL scintillation cocktail (Ultima-Flo AP) and counted for 5 min in a liquid scintillation counter (Tri-Carb 2910 TR, Perkin Elmer). The lipid inositol extract was counted in a liquid scintillation counter ...
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bioRxiv - Biochemistry 2023Quote: ... Each binding reaction was allowed to reach equilibrium at 30 °C for 5 min prior to fluorescence polarization measurements in a Victor X5 Multilabel Plate Reader (Perkin Elmer). Each Kd measurement was calculated according to our previous work (45) ...
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bioRxiv - Microbiology 2024Quote: ... 5 ml cultures were grown in the presence of [32P]-phosphate (final concentration of 1 µCi ml-1; Perkin Elmer NEX060001MC) overnight ...
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bioRxiv - Biophysics 2024Quote: ... A six-point standard curve from 200 ppb to 1 ppb (200, 100, 50, 10, 5, 1) was prepared from elemental standards (Perkin Elmer), plus a 0-ppb blank ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were washed 3 times for 5 min in TBS-T buffer and prior to chemiluminescence detection using Western Lightning chemiluminescent substrate (Perkin Elmer). Chemiluminescence signal was detected with an ECL-Imager (INTAS ...
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bioRxiv - Genetics 2022Quote: ... and the final spectrophotometric reading was carried out by microplate reader (Perkin Elmer Wallac 1420 Victor 2 Multi-Label) set at a wavelength of 450 nm.
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bioRxiv - Biochemistry 2021Quote: The following drugs were used in this study: R1881 (methyltrienolone; used at 2 nM) (PerkinElmer, Inc., Waltham, MA, USA) and hydroxyflutamide (HO-flutamide ...
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bioRxiv - Microbiology 2021Quote: ... 100µL of undiluted PVs were serially diluted 2-fold down a white 96-well F-bottom plates (Perkin Elmer) in 50µL of DMEM ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... coelenterazine 400a was added to a final concentration of 2.5 μM and 2 min later BRET was measured on an EnVision 2104 Multilabel Reader (PerkinElmer) using 410/80 nm (donor ...
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bioRxiv - Microbiology 2020Quote: ... 293FT cells (2 × 104 cells) were plated in 96-well View Plates (Perkin Elmer Life Sciences, Waltham, MA, USA). Five hours later ...
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Secretin receptor as a target in gastrointestinal cancer: expression analysis and ligand developmentbioRxiv - Cancer Biology 2022Quote: ... to a concentration of 200 μM with an addition of 0.5 mCi carrier-free 125I-NaI in 2 μl of carrier solution (PerkinElmer). Iodination was initiated by addition of 4 μl chloramin-T solution (SigmaAldrich ...
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bioRxiv - Immunology 2021Quote: ... The SARS-CoV-2 spike protein antigen (2.0µg/mL) was directly adsorbed on half-area 96-well OptiPlate (96W HB, Perkin Elmer) microplates for 2h at 37°C in a humidified incubator.
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bioRxiv - Cell Biology 2020Quote: ... placed in polistor tubes with 2 ml of Ultima gold™XR liquid scintillation cocktail (Perkin Elmer, Massachusetts, USA) and counted in a scintillation counter ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were split on day 2 and 10,000 cells were seeded in each well (96 well-plate; CellCarrier-96 Ultra Microplates, Perkinelmer). At 12-14 h after cell seeding ...
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bioRxiv - Biochemistry 2021Quote: ... bound to albumin (1:1 molar ratio) or 0.1 μM [1-14C]oleic acid (2 μCi/dish Perkin Elmer) bound to albumin (1:1 molar ratio ...
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bioRxiv - Biochemistry 2021Quote: ... 20 μL of protein mixture was added to each well of a 1/2 area 96-well OptiPlate (PerkinElmer). Compounds were then added from DMSO stock to protein mixture (0.4 μl/well ...
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bioRxiv - Biochemistry 2021Quote: ... 20 μL of protein mixture was added to each well of a 1/2 area 96-well OptiPlate (PerkinElmer). FKBP12F36V competitor or p300-BRD competitor was then added from DMSO stock to protein mixture (0.4 μl/well ...
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bioRxiv - Biochemistry 2021Quote: ... pH 7.4) supplemented with 10% dialyzed FBS in the presence of 10 μCi of myo-[2-3H]inositol (PerkinElmer) for 10 hours ...
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bioRxiv - Cell Biology 2021Quote: ... Worms were imaged on a 2% agarose pad on a Nikon Ti-E Eclipse inverted/UltraVIEW VoX (Perkin Elmer) spinning disc confocal setup ...
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bioRxiv - Microbiology 2022Quote: ... 10 μL of lipid extracts were mixed with 2 mL of Ultima Gold™ scintillation fluid (Perkin Elmer, USA), and radioactive counts were measured using a MicroBeta2® scintillation counter (Perkin Elmer ...
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bioRxiv - Immunology 2023Quote: ... Samples were incubated in triplicate (106 per sample) with 1 µCi of 2-[1,2-3H]-deoxyglucose (20 Ci/mmol; PerkinElmer) in uptake buffer for 4 min at room temperature and immediately spun through a layer of bromo-dodecane into a layer of 20% perchloric acid/8% sucrose to stop the reaction and separate cells from unincorporated 2-[3H]-deoxyglucose ...
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bioRxiv - Microbiology 2023Quote: ... assays were carried out by incubating 500ng of methyltransferases Rv2067c or DOT1L with 1–2 μg of recombinant mammalian histones or MtHU and 80μCi 3H-SAM (PerkinElmer) in a buffer containing 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Neuroscience 2023Quote: Mice were imaged for 2 minutes using a cooled charged-coupled device camera (IVIS Lumina; Perkin Elmer, Coventry, UK). Dams were anaesthetised with isoflurane as above.
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bioRxiv - Biochemistry 2023Quote: ... 2-[1,2-3H(N)]-deoxy-D-glucose and [1,2-14C]-acetic acid were purchased from PerkinElmer (Waltham, MA, USA). PPARγ ...
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bioRxiv - Developmental Biology 2023Quote: ... ORO was measured by extracting lipids with a petrol ether/isopropanol mixture (3:2) and quantified for their absorbance at 490 nm for 0.1 s with Victor Spectrophotometer (PerkinElmer). Standard curve was applied and quantification was expressed in µg of pure ORO powder.
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bioRxiv - Bioengineering 2023Quote: ... FTIR of CNPs was measured in ATR (attenuated total reflectance) mode using a Spectrum 2 FTIR spectrometer from PerkinElmer. The scanning range for the measurements was 1000 cm-1 to 4000 cm-1.
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bioRxiv - Physiology 2023Quote: ... Measurement of radiolabelled 2-deoxyglucose was carried out in a Tri-Carb 4910 TR liquid scintillation analyzer (Perkin Elmer) by adding 200 μl of muscle lysate to 4 ml scintillation fluid (Ultima Gold ...
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bioRxiv - Biochemistry 2024Quote: ... The radioactivity was subsequently counted (counts per minute, CPM) in a scintillation counter (MicroBeta 2 Plate Counter, Perkin Elmer) using a scintillation cocktail (OptiPhase SuperMix ...
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bioRxiv - Biochemistry 2024Quote: ... The radioactivity was measured as counts per minute (CPM) using a scintillation counter (MicroBeta 2 Plate Counter, Perkin Elmer) with a scintillation cocktail (OptiPhase SuperMix ...
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bioRxiv - Cancer Biology 2024Quote: ... CO2 trapping solution was prepared as follow: radiolabeled [1-14C] PA (2 µCi/mL; Perkin Elmer, Cat: #NET043001MC, US) and D-[14C(U)] glucose (2 µCi/mL ...
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bioRxiv - Neuroscience 2021Quote: ... and loaded overnight with MEM +HIHS cell culture media supplemented with 4 μCi/mL D-[2,3-3H]aspartate (Perkin Elmer, Waltham, MA, cat. #NET501001MC). Coverslips were washed in isosmotic Basal medium containing (in mM ...
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bioRxiv - Biochemistry 2021Quote: ... an optimized set of 4 small interfering RNAs (siRNAs; SMARTPool) targeted against human Furin were purchased from Horizon Discoveries (Perkin Elmer, Lafayette, LA, USA) and transfections of HeLa cells were carried out using INTERFERin (PolyPlus ...
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bioRxiv - Cancer Biology 2021Quote: ... For multiplexed immunohistochemistry signals were detected using Opal™ 4-Plex Kit according to the manufacturer’s protocol (catalog no. NEL810001KT; Perkin Elmer, Waltham, MA, USA). For more details see Supplemental Materials section.
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bioRxiv - Microbiology 2020Quote: Purified Caulobacter ParB-His6 or TetR-His6 (final concentration: 25 μM) were incubated with 5 nM radiolabeled P32-α-CTP (Perkin Elmer), 30 μM of unlabeled cold CTP (Thermo Fisher) ...
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bioRxiv - Neuroscience 2020Quote: ... The receptor autoradiography procedure was performed using a linear V1a-R antagonist [125I]-d(CH2)5(Tyr[Me])-AVP (Perkin Elmer, USA) or a linear OXTR antagonist [125I]-d(CH2)5[Tyr(Me)2-Tyr-Nh2]9-OVT (Perkin Elmer ...
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bioRxiv - Plant Biology 2021Quote: ... Seedlings were metabolically labelled with radioactive phosphate by incubating them overnight for ~16-20 hrs with 10 μL (5-10 μCi) carrier-free 32P-PO43- (32Pi; PerkinElmer, The Netherlands) in labelling buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were fixed with 2% PFA at RT for 30 min and stained with the Hoechst 33342 dye (1 μg/ml) at 37 °C and 5% CO2 before imaging with the Operetta™ high content imaging system (PerkinElmer Inc.). Forty fields were acquired from each well using appropriate filter sets ...
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bioRxiv - Molecular Biology 2020Quote: 100 pmol of WT or mutant RNA oligonucleotides (synthesized by Integrated DNA Technologies, Inc.) were 5’- end labeled with [γ32P] ATP (Perkin Elmer, NEG035C001MC) and T4 polynucleotide kinase (New England Biolabs ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were finally washed three times with PBST for 5 min and protein levels were visualized by enhanced chemiluminescence (Perkin Elmer, NEL104001EA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Tumors were isolated from mice and sectioned into 5 micrometer sections for staining with the desired markers (below) using Tyramide Signal Amplification (PerkinElmer, Waltham MA) per manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2021Quote: ... The colonies were grown in 160 μL of TAP medium for 5 days in black clear bottom 96 well plates (CellCarrier-96 Black, 6005550, PerkinElmer Inc, USA), sealed with a paper tape ...
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bioRxiv - Developmental Biology 2024Quote: ... Plates (with lid on) were placed in the Opera Phenix High-Content Screening System (Perkin Elmer; prewarmed to 37C and 5% CO2). Images were collected every 30 min for 24 hours.