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Citations for PerkinElmer :
551 - 600 of 656 citations for Who Coplanar & Mono Ortho Pcb + Pcb 170 & Pcb 180 13C12 99% 20 Ng Ml In N Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: cKO cells grown in the presence or absence of acetamide normalized to OD600 = 1 were incubated for 2 hours at 37 °C in 7H9 media containing [14C]-acetate (final concentration 0.5 μCi/ml; Perkin Elmer). Labeled cells were harvested by centrifugation at 5,000 x g for 3 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... media was replaced with fresh media containing 200 μM ascorbate and 100 mCi/mL of 35S-Cys/Met (Perkin Elmer) for 10 min ...
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bioRxiv - Genetics 2023Quote: ... and subsequently pulsed for 30 minutes in starvation medium supplemented with 200µCi/mL EasyTagTM EXPRESS 35S protein labeling mix (Perkin Elmer). Cells were chased in RPMI supplemented with 0.4 mM methionine for 0 ...
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bioRxiv - Molecular Biology 2023Quote: ... was done using the same procedure for the cold transcription except for the use of 0.5 µl alpha-32P-labeled UTP (3000 Ci/mmol-10 mCi/ml) (Perkin Elmer) and 0.8 mM UTP (instead of 2 mM) ...
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bioRxiv - Genetics 2023Quote: ... A sample of cells were stained with 10 µg ml–1 Hoechst plus 1:500 acridine orange/propidium iodide solution and counted on an Opera Phenix confocal microscope (PerkinElmer). A total of 100,000 cells per well were seeded into neural coating medium-filled 96-well plates in 100 µl per well of neural medium comprising Brainphys medium with 1× B27 plus vitamin A ...
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bioRxiv - Cancer Biology 2024Quote: ... Eµ-Myc lymphoma cells grown in 6-well plates were spiked with 0.2 μCi/mL [1-14C]-acetate (PerkinElmer; NEC084H001MC) for the final 4 h of the experimental period ...
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bioRxiv - Biochemistry 2024Quote: ... were transferred to 1.5 mL Eppendorf tubes containing 10 μL of pre-incubated at 37°C respective radioisotope (3H methionine [0.77 μCi, Perkin Elmer] ...
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bioRxiv - Biophysics 2021Quote: ... Depletion of NADPH is observed in 1 mL cuvettes with a path length of 1cm in a Lambda 650 UV/VIS spectrometer (Perkin Elmer) with attached water Peltier system set to 17°C ...
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An mRNA processing pathway suppresses metastasis by governing translational control from the nucleusbioRxiv - Cancer Biology 2021Quote: ... Cancer cell growth was monitored in vivo at the indicated times by retro-orbital injection of 100 μl of 15 mg/mL luciferin (Perkin Elmer) dissolved in 1X PBS ...
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bioRxiv - Biophysics 2021Quote: ... add 10 mL scintillation solution and measured the radioactivity retained on the membranes in liquid scintillation counter Tri-carb 4910TR (Perkin Elmer) [44].
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bioRxiv - Biophysics 2022Quote: ... Samples were dried for at least 30 mins at 95 °C to remove unfixed 14C before re-suspending the fixed 14C pellets with ultra-pure water and adding 2 mL of scintillation cocktail (Ultima Gold XR, PerkinElmer, US). Radioactivity measurements were then performed using a scintillation counter (Tri-Carb ...
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bioRxiv - Plant Biology 2019Quote: ... the bottom piece (0-0.5 cm) was discarded and the remaining pieces were placed separately into 5 mL Ultima Gold™ (PerkinElmer, # 6013329) for overnight maceration ...
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bioRxiv - Genetics 2021Quote: Cells were fixed in growth medium with 3.7% formaldehyde at room temperature for 30 minutes and their cell walls digested with a solution containing 50 μg/ml zymolyase and 1:50 glusulase (Perkin Elmer). The digested cells were mounted on poly-L lysine coated slides to immobilize them for microscopy ...
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bioRxiv - Microbiology 2019Quote: ... 10% (v/v) glycerol) supplemented with 200 μCi ml−1 (65 nM) of [γ-33P]ATP (PerkinElmer, 3000 Ci mmol−1) for 10 minutes at room temperature in order to obtain the optimal FrzECheA autophosphorylation activity ...
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bioRxiv - Cancer Biology 2020Quote: ... Both supernatant and lysate were moved to separate counting vials and mixed with 3 mL scintillation fluid (Ultima Gold #6013321, Perkin Elmer). 14C levels were measured in each sample using a Packard Liquid Scintillation Analyzer ...
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bioRxiv - Biochemistry 2021Quote: ... All filters were dissolved in 10 ml Emulsifier Safe scintillation fluid and counted using a Tri-Carb A4810TR Liquid Scintillation Analyzer (Perkin Elmer). Experiments were performed in triplicate with fresh cultures.
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bioRxiv - Biochemistry 2021Quote: ... Each filter was placed in 10 ml Emulsifier Safe scintillation fluid and counted using a Tri-CarbA4810TR Liquid Scintillation Analyzer (Perkin Elmer). CodB concentration was quantified based on the GFP fluorescence ...
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bioRxiv - Biochemistry 2020Quote: ... Fifty μl of each elution fraction was suspended in 1 ml of liquid scintillation reagent (Ultima Gold, PerkinElmer, Wellesley, MA, USA), and the radioactivity in each vial was measured using a scintillation counter ...
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bioRxiv - Cell Biology 2021Quote: ... incubated at 37°C for 30 min in 500 μl starvation medium (DMEM lacking methionine and cysteine) and another 30 min in 500 μl labeling medium containing 100 μCi/ml [35S]methionine/cysteine (Perkin Elmer). The cells were washed twice with PBS and lysed in 500 μl lysis buffer (PBS with 1% Triton X-100 ...
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bioRxiv - Microbiology 2020Quote: ... log phase promastigotes were washed twice with PBS and resuspended at 5.0 x 107 cells/ml in PBS in the presence of 100 µM of D-[U-14C] glucose (250 mCi/mmol, PerkinElmer Inc.). The reaction mixture (200 µl each in triplicates ...
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bioRxiv - Plant Biology 2020Quote: ... the remaining root parts were collected and ground completely in liquid nitrogen and homogenized in 1 mL scintillation solution (PerkinElmer, 6013199). The samples were incubated overnight to allow the radioactivity to evenly diffuse into the whole volume of the scintillation cocktail ...
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bioRxiv - Neuroscience 2020Quote: ... the solvent was evaporated under N2 flow and resuspended with 500 mL of chloroform/methanol (2:1v/ v) and half the suspension was placed separately into vials with 5 mL of scintillation solution (Ultima Gold XR, Perkin Elmer). Levels of radioactivity were measured on the Liquid Scintillation Analyzer ...
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bioRxiv - Microbiology 2020Quote: ... CoV-infected cells and control mock-infected cells were incubated for different periods of time with tritiated uridine ([5-3H]uridine, 1 mCi/ml, Perkin Elmer), which was mixed in a 1:1 ratio with double-concentrated medium ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were pulsed with 0.1 mCi/ml 35S-L-methionine/35S-L-cysteine mix (EasyTag™ EXPRESS35S Protein Labeling Mix, Perkin Elmer) in cysteine/methionine-free DMEM for 15 min at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were pulsed with 0.1 mCi/ml 35S-L-methionine/35S-L-cysteine mix (EasyTag™ EXPRESS35S Protein Labeling Mix, Perkin Elmer) in serum-free ...
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bioRxiv - Cell Biology 2020Quote: ... Glass fibre prefilters were air dried for 5 min and the amount of 35S-labeled methionine was analyzed with 5 ml liquid scintillation cocktail Ultima Gold™ (6013329, PerkinElmer) by Liquid Scintilation Analyzer Tri-Carb 4910 TR (PerkinElmer).
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bioRxiv - Plant Biology 2022Quote: ... 5-mm root segments away from the apical 2 mm root tip and 2 mm away from the root-shoot junction were carefully cut and soaked overnight in 4 ml of liquid scintillation fluid (Ultima Gold, PerkinElmer, USA), and the radioactivity was measured with a scintillation counter (model LS6500 ...
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bioRxiv - Neuroscience 2021Quote: ... cells were washed and incubated at 37 °C in phosphate buffered saline (PBS) containing 2 μCi/ml [3H]-glycine (1.6 TBq/mmol; PerkinElmer Life Sciences) isotopically diluted to reach 10 μM final glycine concentration ...
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bioRxiv - Microbiology 2020Quote: ... cells were metabolically pulse labeled with methionine- and cystine-free DMEM supplemented with 70 μCi/ml 35S-labelled Met/Cys (EasyTag EXPRESS35S, Perkin Elmer). Total protein was boiled in SDS sample buffer and then either fractionated by SDS PAGE and exposed to film or precipitated in 10% Trichloroacetic acid (TCA ...
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bioRxiv - Neuroscience 2021Quote: ... was added to the chamber for 30 min to inhibit transcription after which slices were transferred to fresh aCSF containing ~10 mCi/ml [35S] Met/Cys (Perkin Elmer) for an additional 30 min ...
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bioRxiv - Microbiology 2020Quote: ... streptavidin donor beads were added (20 μg/ml) and the mixtures were incubated for 30 mins and were then measured with the Ensight multimode reader (Perkin Elmer) using excitation wavelength of 680 nm with donor beads and measurement of emission wavelength of 615 nm from AlphaLISA anti-HIS acceptor beads ...
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bioRxiv - Physiology 2020Quote: ... Each filter was then placed in a Sterilin™ scintillation vial and radioactivity determined by: addition of 4 mL of Ultima Gold XR liquid scintillant (PerkinElmer), overnight incubation at room temperature and the retained radioactivity determined using a Beckman Coulter LS 6500 Multi-purpose scintillation counter (Beckman Coulter Inc. ...
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bioRxiv - Immunology 2021Quote: ... dissolved in PBS (150 μg/mL) 10min before analysis performed using the IVIS® Lumina II platform (PerkinElmer, Waltham, MA, USA). Photons emitted from luciferase-expressing cells in selected regions of interest (ROI ...
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bioRxiv - Neuroscience 2020Quote: ... cells were incubated in a solution containing an isotopic dilution of 2 μCi/ml [3H] glycine (1.6 TBq/mmol; PerkinElmer Life Sciences) in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% dialyzed fetal calf serum and then metabolically labelled for 10 min with 55 μCi/ml 35S-methionine/cysteine protein labeling mix (Perkin Elmer). Cells were rinsed immediately with PBS twice and solubilized with 1% Triton X-100 in solubilization buffer (50 mM HEPES-KOH ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated 10 minutes at 37 °C by addition of 1 μCi/ml of L-[3,4-3H]-Glutamic acid (Perkin Elmer, NET490) and 10µM L-glutamate (Sigma) ...
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bioRxiv - Plant Biology 2019Quote: ... 5 mm root segments away from the apical 2 mm were carefully cut and soaked for overnight in 4 mL liquid scintillation fluid (ULTIMA GOLD, PerkinElmer, USA), and the radioactivity was measured with a scintillation counter (model LS6500 ...
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bioRxiv - Cell Biology 2020Quote: ... subconfluent HDLECs cultured in 12-well plates were incubated with 1 ml per well EBM2 medium (containing appropriate amounts of serum and supplement) with [5-3H]-glucose (Perkin Elmer) for 2-3 hours ...
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bioRxiv - Cell Biology 2020Quote: MCF10A cells transfected with control or ErbB3 siRNA were incubated with growth factor deprived DMEM media lacking methionine and cysteine supplemented with 10 μCi/ml 35S-methionine/cysteine (Perkin Elmer) for 30 minutes at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... rabbit reticulocyte lysate was incubated with 1μg of the appropriate expression plasmid and 2mL of [35S] L-methionine (1,000 Ci/mMol at 10mCi/mL) (Perkin Elmer, NEG709A) for 90 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... was added at 5 μg/ml and incubated for 5 min before imaging using an IVIS Spectrum imaging system (Perkin Elmer). Data were analyzed with Living Image Software 4.1 (Perkin Elmer).
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bioRxiv - Immunology 2021Quote: ... Liver luminescence was assessed 42 hours post-challenge by intraperitoneally injecting anesthetized mice with 100 μl D-luciferin (30mg/ml) and then determining liver luminescence using an IVIS Spectrum Imaging System (Perkin Elmer). Beginning four days after challenge ...
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bioRxiv - Microbiology 2020Quote: ... The dry filter paper was exposed to film for imaging before the individual dots on the filter paper were excised and scintillated with the addition of 3 ml scintillation fluid (Perkin Elmer). Radioactive counts were measured as scintillation counts per minute.
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bioRxiv - Cell Biology 2020Quote: ... subconfluent HDLECs cultured in 12-well plates were incubated with 1 ml per well EBM2 medium (containing appropriate amounts of serum and supplement) with [6-14C]-glucose (Perkin Elmer) for 6 hours ...
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bioRxiv - Cell Biology 2020Quote: ... subconfluent HDLECs cultured in 6-well plates were incubated with 2 ml per well EBM2 medium (containing appropriate amounts of serum and supplement) with [14C(U)]-glutamine (Perkin Elmer) for 6 hours ...
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bioRxiv - Immunology 2022Quote: ... Paracellular permeability was determined by measuring mucosal-to-serosal flux (%flux/cm2/hour) of the small inert probe 51Cr-EDTA (360 Da; 6 μCi/mL) (Perkin Elmer), by taking an initial radiolabelled sample of mucosal buffer and then samples from the serosal compartment every 30 minutes for 2 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... Then for the 6xHIS-tagged HAT module a 10 µL mixture of 5 µg/mL AlphaLISA Nickel chelate acceptors beads (PerkinElmer #AL108) and 10 µg/mL AlphaScreen donor beads (PerkinElmer #6760002) ...
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bioRxiv - Microbiology 2022Quote: 643nt pre-crRNA was in vitro transcribed with [a-32P]-ATP (10 mCi ml-1, 3000 Ci mmol-1, Perkin Elmer) as described above ...
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bioRxiv - Neuroscience 2022Quote: ... 0.5 mL the top aqueous layer was used to count the radioactivity using a Tri-Carb 2800TR Liquid Scintillation Analyzer (Perkin Elmer). 0.5 mL medium at time point 0 was used as a control to determine the specific activity of the buffer.
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bioRxiv - Plant Biology 2023Quote: ... Stained SDS-PAGE bands were excised and transferred to scintillation vials and emulsified in 10 ml of Ultima Gold XR scintillation liquid (Perkin Elmer) in darkness at room temperature overnight ...