Labshake search
Citations for PerkinElmer :
551 - 600 of 629 citations for 8 4 Chlorophenoxy 2 methylene octanoic acid L prolinamide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Physiology 2024Quote: ... or 120 nM of insulin in fresh serum-starvation medium for 30 min before adding 2 μL.mL-1 D-[14C(U)] glucose (NEC042B005MC; Perkin Elmer) for a further 90 min ...
-
bioRxiv - Genetics 2019Quote: ... were mounted on infrared windows where spectral images were collected at a 4 cm-1 spectral resolution and ~7 μm spatial resolution from a Spotlight 400 Imaging system (Perkin Elmer Instruments, Shelton, CT USA). Background spectra were collected under identical conditions from clear Ba2F windows and subtracted from sample data by instrumental software ...
-
bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated with a D1R antagonist for 1h at 4°C in a solution equivalent to the pre-incubation buffer with the addition of 1nM [3H]SCH23390 (Perkin Elmer, USA, 85 Ci/mmol). Non-specific binding was determined by incubating sections under the same conditions with excess amount of the D1R ...
-
bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated with a D2R antagonist for 1h at 4°C in a solution equivalent to the pre-incubation buffer with the addition of 4nM [3H]raclopride (Perkin Elmer, USA, 60.1 Ci/mmol). Non-specific binding was determined by incubating sections under the same conditions with excess amount of the D2R ...
-
bioRxiv - Biophysics 2022Quote: ... Fluorescence polarization measurements were carried out in black 96-well plates measured on a Wallac Victor 2 Plate Reader (Perkin Elmer). For all measurements peptide was held at concentrations of 50 nm (or 100nM ...
-
bioRxiv - Biophysics 2022Quote: ... Samples were dried for at least 30 mins at 95 °C to remove unfixed 14C before re-suspending the fixed 14C pellets with ultra-pure water and adding 2 mL of scintillation cocktail (Ultima Gold XR, PerkinElmer, US). Radioactivity measurements were then performed using a scintillation counter (Tri-Carb ...
-
bioRxiv - Cell Biology 2021Quote: ... was monitored every 5 min for 2 h at 37°C using a fluorescent plate reader (EnSpire Multimode Plate Reader, Perkin Elmer). Samples were assayed in technical triplicates and in biological replicates as indicated in the figure legend.
-
bioRxiv - Cell Biology 2021Quote: ... 500nl of 2 µM siRNAs (20nM final concentration) were distributed on 6 different 384-well imaging plates (CellCarrier Ultra, Perkin Elmer), as described in Table 3 ...
-
bioRxiv - Microbiology 2022Quote: ... Cytotoxicity was measured by adding resazurin [alamar blue (ThermoFisher)] diluted to 1x in DMEM according to the manufacturor’s protocol and incubated on cells for 2-4hr and reading fluorescence on a Victor 1420 Multilabel plate reader (Perkin Elmer) with excitation at 560 nM and emission at 590 nM.
-
bioRxiv - Neuroscience 2021Quote: ... cells were washed and incubated at 37 °C in phosphate buffered saline (PBS) containing 2 μCi/ml [3H]-glycine (1.6 TBq/mmol; PerkinElmer Life Sciences) isotopically diluted to reach 10 μM final glycine concentration ...
-
bioRxiv - Neuroscience 2020Quote: ... cells were incubated in a solution containing an isotopic dilution of 2 μCi/ml [3H] glycine (1.6 TBq/mmol; PerkinElmer Life Sciences) in PBS ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... and the amount of tritiated acetate in the organic phase was measured by liquid scintillation counting for 2 min (Tri-Carb 2900TR, Perkin Elmer). Each sample was assayed in triplicate and counts were corrected for background by subtraction of counts obtained using whole blood samples devoid of cholinesterase activity and treated in a similar fashion ...
-
bioRxiv - Plant Biology 2019Quote: The concentration of PINA was calculated by measuring optical density at 280 nm with a UV-spectrophotometer (Perkin Elmer Lambda 2) and by using an extinction coefficient of PINA (31.10 M−1 cm−1 or 2.376 g−1 L.cm−1) ...
-
bioRxiv - Physiology 2021Quote: ... a longer time was required to achieve steady state) a single bolus of 12 μCi of [1-14C]-2-deoxy-D-glucose (Perkin Elmer) in 96 μl of saline was administered ...
-
bioRxiv - Immunology 2021Quote: ... brain and testis tissue samples from hACE2 transgenic mice challenged intranasally with SARS-CoV-2-nLuc (1 x 105 FFU; 6 dpi) were imaged after necropsy using bioluminescence imaging (IVIS, Perkin Elmer), pruned to isolate regions with high nLuc activity and immediately pre-fixed with 3 % glutaraldehyde ...
-
bioRxiv - Biochemistry 2021Quote: Polyacrylamide gel slices (1-2 mm) containing the purified proteins were prepared for mass spectrometric analysis using the Janus liquid handling system (PerkinElmer, UK). Briefly ...
-
bioRxiv - Physiology 2020Quote: On the fifth day of EOS or PBS treatment 3H-2-deoxy-D-glucose (2DG; 10 uCi/mouse; PerkinElmer, Waltham, MA) was given to 4-hour fasted individually housed mice ...
-
bioRxiv - Molecular Biology 2020Quote: Nascent proteins in U-2 OS cells expressing GFP-G3BP1 were labeled with 35S-met and -cys (EXPRE35S35S Protein Labeling Mix, Perkin Elmer) for 30 minutes in the presence of DMSO (0.1%) ...
-
bioRxiv - Neuroscience 2019Quote: ... Sections were rinsed 3 times for 2 min in PBS-Tx and incubated in tyramide signal amplification (TSA) blocking reagent (PerkinElmer, FP1012) in PBS-Tx (TSA-block ...
-
bioRxiv - Developmental Biology 2021Quote: ... Successfully mounted embryos were then imaged starting between 2 and 7 ss using a 20X objective on a Perkin-Elmer UltraVIEW Vox spinning disk confocal microscope (Perkin Elmer) while maintained at 32°C ...
-
bioRxiv - Cell Biology 2021Quote: The data was collected in a set of 2 experiments for 9 compounds as follows: 384 well plates (PE cell carrier ultra, PerkinElmer, 6057308) containing 12,000 cells per well in RPMI1640 medium (Sigma ...
-
bioRxiv - Microbiology 2020Quote: Infected mice were anesthetized using isofluorane and 1 μl of decyl aldehyde was instilled into each nostril before placement in a BSL-2 chamber within the IVIS instrument (IVIS Spectrum, Perkin Elmer). Isofluorane anesthesia was sustained during IVIS imaging ...
-
bioRxiv - Molecular Biology 2021Quote: ... total RNA was separated in 2% glyoxal agarose gels and blotted onto a positively charged nylon membrane (GeneScreen Plus, Perkin Elmer). [32P]-labelled probes specific for each transcript were generated by linear PCR ...
-
bioRxiv - Cell Biology 2020Quote: ... subconfluent HDLECs cultured in 6-well plates were incubated with 2 ml per well EBM2 medium (containing appropriate amounts of serum and supplement) with [14C(U)]-glutamine (Perkin Elmer) for 6 hours ...
-
bioRxiv - Immunology 2021Quote: ... Samples were then incubated at room temperature for 2 hours and incorporated radioactivity analyzed on a 1450 MicroBeta TriLux Microplate Scintillation and Luminescence counter (Perkin Elmer).
-
bioRxiv - Neuroscience 2022Quote: ... 1:200 in TNB buffer or NGS for 2 hours at room temperature and then incubated with HRP-Conjugated Streptavidin (PerkinElmer; USA) 1:100 in TNB buffer or NGS for 30 min ...
-
bioRxiv - Microbiology 2022Quote: ... Fluorescence was measured for 90 minutes at 2-min intervals using a 340/460 excitation/emission filters on a Envision plate reader (Perkin Elmer). The IC50 values were calculated by fitting the curves using a four-parameter equation in GraphPad Prism ...
-
bioRxiv - Microbiology 2022Quote: ... Cells (2 × 104 cells/well) were seeded into 96-well plates (Cell Carrier-96well Ultra microplate; PerkinElmer Inc., Waltham, MA, USA) and differentiated to macrophages with phorbol 12-myristate 13-acetate (10 ng/mL ...
-
bioRxiv - Biochemistry 2022Quote: ... A competitor unlabelled DNA of the same sequence was added at 2 µM and FP was measured over time on a VictorV plate reader (Perkin Elmer). Experiments with PARP1 were carried out in the same conditions as for PARP2 with the exception that the reaction buffer which was 12 mM Hepes pH 8.0 ...
-
bioRxiv - Genetics 2022Quote: ... Probe for southern blot was amplified with primers listed in table S1 and then labeled using [α-32P] 2’-deoxycytidine 5’-triphosphate (dCTP) (PerkinElmer) with Klenow fragment (3’-5’ exo- ...
-
bioRxiv - Synthetic Biology 2022Quote: ... Mice were monitored for disease progression 1-2 times per week via bioluminescence imaging (BLI) using an IVIS imaging system (Perkin Elmer). For CHLA-255 models ...
-
bioRxiv - Microbiology 2022Quote: ... 2 x 105 parasites were applied to each well of a clear-bottomed 96-well plate (Perkin Elmer cat. no. 6055300) seeded with HFF cells ...
-
bioRxiv - Physiology 2023Quote: ... the animals were fasted overnight and a bolus of 2-deoxy-D-[1,2-3H]-glucose (1mC/ml; specific activity: 20Ci/mmol; PerkinElmer, Madrid, Spain) mixed with glucose (100µCi/kg body weight ...
-
bioRxiv - Molecular Biology 2023Quote: ... the reaction was initiated by the addition of 3 μl of 2 mM [ψ-32P]ATP (222 TBq/mmol Perkin Elmer) to the mixture (final concentration ...
-
bioRxiv - Biochemistry 2023Quote: ... 690 uL of the samples were transferred to a scintillation vial with 2 mL of Insta-Gel Plus scintillation cocktail (Perkin Elmer). Radioactivity was measured with the Scintillation counter (Perkin Elmer) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Immuno-complexes were incubated for 20 min at 30°C in 30 μl of kinase buffer with a final concentration of 50 μM ATP and [γ-32P] ATP (1 x 10−2 μCi/pmol; PerkinElmer). Reactions were stopped by adding 5X LB ...
-
bioRxiv - Microbiology 2024Quote: ... 2×106 bacteria/mL in PBS were seeded in 384-well black clear-bottom plate (ViewPlate®-384 F TC, PerkinElmer) in the presence of 12.5 % BRC ...
-
bioRxiv - Neuroscience 2023Quote: ... The experiments were initiated by placing the choroid plexus in HEPES-aCSF containing 1 μCi/ml 86Rb+ (022-105721-00321-0001, POLATOM, as a tracer for K+ transport) and 4 μCi/ml 3H-mannitol (Perkin Elmer, as an extracellular marker [26]) at 37°C for 2 min in the absence or presence of 2 mM ouabain (O3125 ...
-
bioRxiv - Bioengineering 2020Quote: ... In vivo and ex vivo fluorescence imaging was carried out using a planar (2-D) IVIS Spectrum device (PerkinElmer, Waltham, MA, USA). Pymol (1.7 ...
-
bioRxiv - Neuroscience 2020Quote: ... were harvested in Schneider’s medium on ice and transferred to Schneider’s medium containing 35S-methionine/cysteine (2 µCi/µl; Perkin Elmer Labs Inc., NEG709A) and 30 µM chloroquine (to inhibit autophagy) ...
-
bioRxiv - Cell Biology 2021Quote: ... Then it was mixed with reaction B which contain 5 μg of the GST-substrate in 5x Kinase buffer containing 2 μCi γ-32P-ATP (PerkinElmer, BLU002Z250UC) in 10-μl reactions ...
-
bioRxiv - Cell Biology 2021Quote: RAW cells were incubated for 24 h with inositol-free DMEM (MP Biomedica, CA) containing 10 μCi/ml myo-[2-3H(N)] inositol (Perkin Elmer, MA), 1X insulin-transferrin-selenium-ethanolamine (Gibco) ...
-
bioRxiv - Immunology 2021Quote: ... Virus-mAb/plasma-containing media was then aspirated from cells and 100 μL of a 1:2 dilution of SteadyLite Plus (Perkin Elmer, 6066759) in PBS with Ca++ and Mg++ was added to cells ...
-
bioRxiv - Microbiology 2021Quote: ... 200 μL of each phase were collected and measured in a 2480 automatic Gamma counter Wizard 2 3” (PerkinElmer, Waltham, MA, USA). LogD value were calculated using Excel by dividing measured values of octanol by water and logarithmize the result ...
-
bioRxiv - Microbiology 2021Quote: In all our experiments photon emission of the luminescent bacteria was recorded at regular intervals under general anesthesia (isoflurane, inspired fraction=2%) using IVIS Spectrum and analyzed with Living Image software (PerkinElmer, Richmond, CA) as previously described (Debarbieux et al. ...
-
bioRxiv - Immunology 2021Quote: ... Virus-mAb/plasma-containing media was then aspirated from cells and 100 μL of a 1:2 dilution of SteadyLite Plus (Perkin Elmer, 6066759) in PBS with Ca++ and Mg++ was added to cells ...
-
bioRxiv - Developmental Biology 2021Quote: ... the RNAscope Multiplex Fluorescent Reagent Kit (v.2) and TSA Plus reagents (Perkin Elmer; 1:500 dilution for Cy3 and Cy5 dyes) or Opal dyes (Akoya Biosciences ...
-
bioRxiv - Microbiology 2021Quote: ... Virus-mAb-containing media was then aspirated from cells and 50 μL of a 1:2 dilution of SteadyLite Plus (Perkin Elmer, 6066759) in PBS with Ca++ and Mg++ was added to cells ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Luciferase activity was measured for 2 seconds in intact living cells using a microBeta Wallac luminometer (Perkin Elmer, Villebon sur Yvette, France). Tests were performed in quadruplicate in at least three independent experiments and data were expressed as mean ± s.e.m ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... The OptiPlate-384 plate was incubated at room temperature in a Biosolutions AmbiStore™ D (HighRes) for 2 hours before the HTRF signal was measured on an EnVision® HTS plate reader (PerkinElmer). For OSM-induced CCL2 assay in hit validation step ...