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Citations for PerkinElmer :
501 - 550 of 760 citations for Serum amyloid A 2 protein SAA2 Antibody HRP since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Protein biosynthesis rates were determined by measuring the incorporation of radioactive methionine in auranofin-treated and untreated samples after protein precipitation with 20 % (w/v) trichloro acetic acid using a scintillation counter (Tri-Carb 2800TR, PerkinElmer). 55 µg of protein from pulse-labeled samples for analytical gels and 300 µg of protein from unlabeled samples for preparative gels were rehydrated into IPG strips (24 cm ...
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bioRxiv - Genetics 2023Quote: ... and subsequently pulsed for 30 minutes in starvation medium supplemented with 200µCi/mL EasyTagTM EXPRESS 35S protein labeling mix (Perkin Elmer). Cells were chased in RPMI supplemented with 0.4 mM methionine for 0 ...
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bioRxiv - Cell Biology 2024Quote: ... cells were changed into 1 mL of starvation media containing 200 μCi/mL EasyTag EXPRESS35S Protein Labeling Mix (PerkinElmer NEG772002MC), grown for indicated time points ...
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bioRxiv - Plant Biology 2024Quote: ... Labeling was initiated by adding 15 μL of 35S-Met/Cys (EasyTag Express 35S Protein Labeling Mix, >1000 Ci/mmol, 11 mCi/mL, PerkinElmer). After 20 min ...
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bioRxiv - Plant Biology 2024Quote: ... machine to predict seed protein and oil on a dry matter basis using a global calibration set provided by PerkinElmer Inc.
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bioRxiv - Molecular Biology 2024Quote: DBS were acquired from consenting healthy volunteers via a finger prick onto blood sampling papers (ADVANTEC PKU-S; ADVANTEC; Whatman 903 Protein Saver Cards, Whatman; PerkinElmer 226 Spot Saver Card; PerkinElmer) and dried overnight.
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bioRxiv - Neuroscience 2020Quote: ... animals were anesthetized (2 % isofluorane gas in O2) and luminescence was recorded (IVIS Spectrum, Perkin Elmer, Waltham, MA). Images were analyzed with Living Image Software (Caliper LifeSciences).
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bioRxiv - Plant Biology 2020Quote: ... N was quantified in approximately 2 mg subsamples using a CHN elemental analyzer (EA2400, PerkinElmer, Waltham, MA, USA). For phosphorus content ...
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bioRxiv - Neuroscience 2020Quote: HUVECs were incubated for 6 hours in EGM-2 containing 0.4 μCi/ml [5-3H]-D-glucose (PerkinElmer). Supernatant was transferred into glass vials containing perchloric acid and sealed with rubber stoppers ...
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bioRxiv - Molecular Biology 2020Quote: ... In brief, using a rabbit antimelatonin antiserum (PF1288; P.A.R.I.S., Paris, France) and 2-Iodomelatonin (NEX236050UC; PerkinElmer, Boston, Massachusetts) as tracer RIA was performed ...
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bioRxiv - Bioengineering 2022Quote: ... mice were shaved then anesthetized with 1–2% isoflurane using a calibrated vaporizer inside an IVIS Spectrum (PerkinElmer). Non-invasive longitudinal monitoring of tumour progression and nanoparticle release was followed by scanning mice with the IVIS Spectrum-bioluminescent and fluorescent imaging system (PerkinElmer) ...
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bioRxiv - Microbiology 2024Quote: ... 200 μl of each fraction was added to 2 ml of Ultima Gold liquid scintillation cocktail (Perkin Elmer), and the radioactivity was measured by a LS-6500 scintillation counter (Beckman) ...
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bioRxiv - Biophysics 2022Quote: ... and incubated at 40 C for 2 h before counting with a MicroBeta scintillation top counter (Perkin Elmer).
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed twice for 2 minutes with wash buffer and incubated in Opal 520 (Perkin Elmer FP1487A) or vivid 520 (ACDbio 323271 ...
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bioRxiv - Biochemistry 2023Quote: ... the beads were suspended in 25 µl of cytoplasmic buffer containing 2 µCi of 3H-labeled SAM (PerkinElmer) and incubated overnight at 32 °C without shaking ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed twice for 2 minutes with wash buffer and incubated in Opal 520 (Perkin Elmer FP1487A) for 30 minutes at 40°C ...
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bioRxiv - Molecular Biology 2024Quote: ... cAMP- dependent luminescence was then measured at 2-min intervals using a PerkinElmer Envision 2104 plate reader (PerkinElmer), as described [24] ...
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bioRxiv - Cell Biology 2020Quote: ... Antibody binding was visualized using ECL Plus Western Lightning reagent (Perkin Elmer NEL 102) and blots were exposed to film (Kodak BioMax MR Film ...
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bioRxiv - Cell Biology 2024Quote: ... Membrane-bound secondary antibody was detected using Western Lightning ECL Plus (#NEL103E001EA, Perkin Elmer) and Medical X-Ray blue films (#FA158 ...
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bioRxiv - Genetics 2024Quote: ... Horseradish peroxidase or biotin-conjugated secondary antibodies and a Tyramide signal amplification kit (PerkinElmer) were used after incubating the primary antibodies ...
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bioRxiv - Immunology 2024Quote: ... 1:20000 Eu3+ cryptate conjugated to a His6-specific antibody (HTRF donor, PerkinElmer AD0402) and 1:500 Streptavidin-XL665 (HTRF acceptor ...
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bioRxiv - Cancer Biology 2021Quote: The intrinsic and extrinsic fluorescence emission spectra of the proteins were measured using an LS55 spectrofluorometer (Perkin Elmer, Waltham MA, USA). WT or N16D HsTPI (0.5 mg·mL-1 ...
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bioRxiv - Cell Biology 2020Quote: ... the PVDF membrane was washed with TBST and incubated with appropriate secondary antibodies (horseradish peroxidase-conjugated goat anti-mouse or anti-rabbit IgG), followed by enhanced chemiluminescence (ECL; Visual protein, Taipei, Taiwan) and the membrane was examined by ECL spectrophotometry (Perkin Elmer, Geliance 600 Camera Imaging system ...
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bioRxiv - Bioengineering 2022Quote: Saturation assays for binding of K1K1H6 to recombinant MET-Fc protein were performed in 384-well microtiter plates (OptiPlate™-384, PerkinElmer© ...
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bioRxiv - Molecular Biology 2021Quote: ... LPS-stimulated plasmablasts were starved for methionine and cysteine for 60 minutes and pulse labeled for 15 minutes with 55 µCi/1×106 cells of Express 35S protein labeling mix (Perkin Elmer). Samples were then diluted 10x with cell culture media containing 5mM unlabeled methionine and cysteine ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane was transferred to a tube and the protein-bound [α-32P]dCTP was quantified in a Beckman Coulter LS 6500 Liquid Scintillation Counter using OptiPhase HiSafe 3 (PerkinElmer) as solvent.
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bioRxiv - Biochemistry 2021Quote: ... and mRNA was translated for 1 hour at 30 °C in presence of 10 µCi/µL EasytagTM 35S Express Protein Labeling Mix (Perkin Elmer). The mixtures were centrifuged for 2 min at 10,000 x g at 4 °C after inhibiting protein synthesis with 1 mM cycloheximide and translocated CFTR was retrieved from the pellet fraction ...
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bioRxiv - Cell Biology 2021Quote: ... Radioactive counts from the media that remain soluble after TCA precipitation (peptide fragments) and pellets from the cells (total non-degraded cell proteins) were measured in a TopCount radioactivity detector (Perkin Elmer). A “protein degradation index” was calculated as the ratio between the counts detected in the soluble media and the counts detected in the pellet cell fraction (82).
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bioRxiv - Cell Biology 2021Quote: ... The cells were pulsed with 0.1 mCi/ml 35S-L-methionine/35S-L-cysteine mix (EasyTag™ EXPRESS35S Protein Labeling Mix, Perkin Elmer) in cysteine/methionine-free DMEM for 15 min at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were pulsed with 0.1 mCi/ml 35S-L-methionine/35S-L-cysteine mix (EasyTag™ EXPRESS35S Protein Labeling Mix, Perkin Elmer) in serum-free ...
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Development of a small molecule that corrects misfolding and increases secretion of Z α1-antitrypsinbioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were pulsed (0.45MBq/106 cells) for 10 min with 35S Cys/Met (EasyTagTM Express Protein Labelling, Perkin Elmer, Beaconsfield, UK) in DMEM without Cys/Met ...
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bioRxiv - Microbiology 2020Quote: ... and adhesin were optimized by finding the hook point for the interaction by setting up a matrix of proteins in 96 well AlphaPlates (Perkin Elmer) in 10 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... with undigested pOTB7/PDIP38 as template and 11 μCi of [35S]Met/CysEXPRE35S35S protein labelling mix (specific activity of >1000 Ci/mmol) from Perkin Elmer. Protein Assay (Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... cells were starved for cysteine and methionine for 15-30 min and pulse labeled for 5 min with 55 µCi/ 35-mm dish of Easytag express 35S protein labeling mix (Perkin Elmer). Where indicated (+DTT) ...
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bioRxiv - Microbiology 2021Quote: ... After these incubations the membranes were washed again with PBS-T and proteins were visualized by Western Lightning Chemiluminescence Reagent Plus (Perkin Elmer).
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bioRxiv - Physiology 2020Quote: ... glycosylphosphatidylinositol-anchored high-density lipoprotein binding protein 1 (GPIHBP1) knock out mice were orally gavaged with 100 μCi of [9,10-3H(N)]-Triolein (Perkin Elmer, NET431001MC) suspended in olive oil ...
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bioRxiv - Immunology 2021Quote: ... The radioactive counts (cpms) of immunoprecipitated protein was quantified using a 96-well Microbeta Trilux liquid scintillation plate reader (Perkin Elmer). Antibody index for each sample was calculated as follows ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were pulse labeled for 15 minutes with 132 μCi/6 cm dish with EasyTag Express 35S Protein Labeling Mix (Perkin Elmer). Radiolabeling was stopped by adding excess ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were pulsed with 0.1 mCi/ml 35S-L-methionine and 35S-L-cysteine mix (EasyTag™ EXPRESS35S Protein Labeling Mix, Perkin Elmer) in methionine- and cysteine-free DMEM for 15 min ...
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bioRxiv - Cancer Biology 2023Quote: ... then each well was incubated for 30 min at 37 °C with 5 μl/well EXPRE35S35S Protein Labeling Mix (PerkinElmer NEG072002MC), after which cells were lysed in RIPA lysis buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: The recruitment of β-arrestin2 by MOR agonists after G protein activation was assessed using the Cisbio HTRF-based β-arrestin2 recruitment assays (Cisbio Assays, PerkinElmer), which monitored the interaction between β-arrestin2 and AP2 ...
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bioRxiv - Molecular Biology 2024Quote: ... or from 10 µg total RNA from gravid adults (for analysis of protein-coding genes near dysregulated piRNAs on Chromosome IV) using the stranded NEXTFLEX Rapid Directional RNA-seq kit (Perkin Elmer). The embryonic and larval libraries represented two independent biological replicates of wild type and ebax-1(tm2321 ...
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bioRxiv - Biochemistry 2024Quote: ... Fluorescence intensity of this protein-free promoter sample was continuously measured for 120 s with a LS-55 fluorescence spectrofluorometer (Perkin Elmer) at 24°C ...
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bioRxiv - Biochemistry 2024Quote: We load equal amounts of 20 µl sample volume for the total protein concentration titration in 384 well plates (PhenoPlate, PerkinElmer, USA). The well plates were chosen due to the superior non adhesive properties that allow to assume spherical shape of the condensates ...
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bioRxiv - Genetics 2022Quote: ... and the final spectrophotometric reading was carried out by microplate reader (Perkin Elmer Wallac 1420 Victor 2 Multi-Label) set at a wavelength of 450 nm.
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bioRxiv - Neuroscience 2020Quote: ... or a linear OXTR antagonist [125I]-d(CH2)5[Tyr(Me)2-Tyr-Nh2]9-OVT (Perkin Elmer, USA) as tracers ...
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bioRxiv - Biochemistry 2021Quote: The following drugs were used in this study: R1881 (methyltrienolone; used at 2 nM) (PerkinElmer, Inc., Waltham, MA, USA) and hydroxyflutamide (HO-flutamide ...
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bioRxiv - Microbiology 2021Quote: ... 100µL of undiluted PVs were serially diluted 2-fold down a white 96-well F-bottom plates (Perkin Elmer) in 50µL of DMEM ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... coelenterazine 400a was added to a final concentration of 2.5 μM and 2 min later BRET was measured on an EnVision 2104 Multilabel Reader (PerkinElmer) using 410/80 nm (donor ...
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bioRxiv - Microbiology 2020Quote: ... 293FT cells (2 × 104 cells) were plated in 96-well View Plates (Perkin Elmer Life Sciences, Waltham, MA, USA). Five hours later ...