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Citations for PerkinElmer :
501 - 550 of 575 citations for Mouse anti Plasmodium falciparum HRP 2 antibody M303 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Detection reagents were incubated with reaction time points for 2 hours at room temperature in 384 well white microplates (PerkinElmer OptiPlate-384) then TR-FRET emission at 665 nm and 615 nm by 320 nm excitation with 50 μs delay and 100 μs integration time was measured using a Molecular Devices SpectraMax M5e plate reader ...
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bioRxiv - Cell Biology 2023Quote: ... Plates were shaking at 999 rpm and 28°C and growth was measured by monitoring OD600 every 2 h for 10 h using a plate reader (2300 EnSpire™, Perkin Elmer).
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bioRxiv - Molecular Biology 2023Quote: ... and the amount of 2-NBDG was measured by determining the fluorescence intensities at Ex/Em = 475/550 nm using an ARVO X2 (PerkinElmer Co., Ltd.). To investigate the effect of β-hydroxybutyrate on glucose uptake ...
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bioRxiv - Physiology 2024Quote: ... Measurement of radiolabeled 2-deoxyglucose was carried out in a Tri-Carb 4910 TR liquid scintillation analyzer (Perkin Elmer, Waltham, MA, USA) by adding 200 μL of muscle lysate to 4 mL scintillation fluid (Perkin Elmer ...
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bioRxiv - Biochemistry 2024Quote: ... whereafter lysed cells were added to 2 mL scintillation fluid and radioactivity was measured using a scintillation counter (Perkin Elmer 2450 Microbeta2). Data were analyzed using GraphPad Prism 8.0.0 and fitted to a four-parameter logistic curve from where the IC50 and the 95% confidence interval were calculated ...
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bioRxiv - Developmental Biology 2024Quote: ... the University of Liverpool’s centre of preclinical imaging provided the imaging services using the Quantum GX-2 system (PerkinElmer, Inc. Waltham, MA). Whole-body scan images were acquired with the protocol FOV72 ...
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bioRxiv - Immunology 2021Quote: ... The mean positive area was determined using appropriate isotype control antibodies and quantified using Volocity (Perkin Elmer) and Fiji (NIH ...
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bioRxiv - Immunology 2019Quote: ... Mono-color staining of each of the antibodies was performed using the OPAL 7 color kit (PerkinElmer), using the same protocol as IHC ...
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bioRxiv - Neuroscience 2020Quote: ... For CC3 staining secondary antibody was followed by signal amplification using TSA Plus Cyanine 3 System (PerkinElmer). Following the incubation with the secondary antibody ...
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bioRxiv - Cancer Biology 2021Quote: ... Antigen-antibody complexes were detected with West Pico Chemi luminescent substrate using standard manufactures protocol (Perkin Elmer). Imaging of all western blots was performed using an UVP BioImager 600 system equipped with VisionWorks Life Science software (UVP ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by primary antibody and corresponding secondary horseradish peroxidase-conjugated polymer (Zytomed Systems, Germany or Perkin Elmer). Each horseradish peroxidase-conjugated polymer mediated the covalent binding of different fluorophore using tyramide signal amplification ...
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bioRxiv - Neuroscience 2023Quote: ... the binding sites of the secondary antibodies were detected using Western Lightning ECL Pro (PerkinElmer, MA, USA). Chemiluminescence was visualized using the Fusion Solo S imaging system (Vilber Bio Imaging ...
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bioRxiv - Cancer Biology 2024Quote: ... The tumor burden was quantified weekly by bioluminescent signal intensity on isoflurane-anesthetized mice that received a subcutaneous injection of D-luciferin (150 μg luciferin/1 g mouse body weight) 10 min prior to IVIS® imaging (PerkinElmer, Waltham, MA,USA). Survival data were presented and reported in Kaplan–Meier plots.39
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bioRxiv - Biochemistry 2021Quote: ... 0.4 μg of the Anti-Myc coated Acceptor Beads (PerkinElmer, MA, USA) was added in 12.5 μl reaction buffer B (25 mM HEPES ...
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bioRxiv - Immunology 2022Quote: ... purified anti-IFNγ and BsAb were radiolabeled with 125I-radionuclide (Perkin Elmer) using Iodogen reaction and then purified by P-6 spin column (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... 10 µL of anti-6X His acceptor beads (Perkin Elmer, reference AL178) were then added and after 1h of incubation at RT with rotation ...
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bioRxiv - Cancer Biology 2021Quote: Bioluminescence Imaging and Quantification: Mice were anesthetized with isoflurane (2-3% in 100% oxygen) and bioluminescence images acquired with an IVIS Spectrum (Perkin Elmer, Waltham, MA) 5 minutes after intraperitoneal injection of Potassium D-Luciferin solution (Perkin Elmer ...
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bioRxiv - Immunology 2021Quote: ... were plated in 3 ml of complete medium supplemented with IL-2 (20 IU/ml) and incubated with 1 μl of 3H-labeled ROL (PerkinElmer, Boston, MA, USA) for 4 h ...
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bioRxiv - Physiology 2021Quote: ... were plated per well in 96-well white microplates and 100 nM of GnRH (or vehicle) were injected using Synergy 2 Multi-Mode Microplate Reader (Bio Tek) or Victor X light plate reader (Perkin Elmer Life Sciences). Luminescence signals were recorded for 30 seconds ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... All the videos (2 min, 4 frames s-1) were acquired at 37 °C using a Nikon TIRF microscopy system (Perkin Elmer, Waltham, MA) on an inverted Ti microscope equipped with a 100× objective and an ImageEM C9100-13 camera (Hamamatsu Photonics ...
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bioRxiv - Microbiology 2019Quote: ... At 6 hpi the cells were starved in a glucose-free medium for 30 min followed by labeling with 200 µCi/ml of mannose-D-[2-3H(N)] (Perkin Elmer, Shelton, CT) for 1.5 h ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... After 6-24 h at 37 °C (as specified in each figure legend) the cell medium was aspirated and substituted with serum-free cell medium containing [3H]thymidine (2 μCi/ml, Perkin Elmer, Waltham, MA). The incubation was continued for 30 min at 37 °C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... lung tissues harvested from euthanized mice were soaked for 10 min in pre-warmed (37°C) PBS with 2% FBS and a 1:10 dilution of Xenolight RediJect Coelenterazine h (Cat# 706506, Perkin Elmer, Waltham, MA). Following a 12-15 min incubation ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were lysed by adding 0.2 mL Optiphase Supermix cocktail and accumulated [3H]DA-radioactivity was measured using a microplate scintillation counter (MicroBeta2 LumiJET, PerkinElmer, Waltham, MA, USA). DAT-specific [3H]DA transport was calculated as the difference in counts obtained in the presence or absence of the DAT-specific antagonist GBR12909 (100 nM) ...
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bioRxiv - Cell Biology 2023Quote: All the videos (2 min, 4-5 frames s-1) were acquired at 37°C using a Nikon TIRF microscopy system (Perkin Elmer, Waltham, MA) on an inverted Ti microscope equipped with a 100× objective and an ImageEM C9100-13 camera (Hamamatsu Photonics ...
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bioRxiv - Neuroscience 2023Quote: ... the same cell samples were further lysed and digested with 2% HNO3 for intracellular iron content quantification using an ICP-AES instrument (Perkin Elmer Optima 8000).
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bioRxiv - Cancer Biology 2023Quote: ... and the plate was incubated in the dark for 2 h and then read in an EnSpire Alpha multimode plate reader (PerkinElmer, Waltham, MA, USA).
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bioRxiv - Immunology 2024Quote: ... After an additional 2 hours blood was collected and serum FITC-dextran levels were measured with a Victor V multiparameter plate reader (Perkin Elmer, Waltham, MA). Quantification of serum FITC-dextran levels was performed with the WorkOut 2.5 software by extrapolation against know concentrations of FITC-dextran standard.
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bioRxiv - Cancer Biology 2019Quote: ... Multispectral panels including the above antibodies were developed and images captured on a Vectra Polaris Instrument (Perkin Elmer). Phenotype counts were determined using InFORM software (Perkin Elmer).
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bioRxiv - Immunology 2020Quote: ... The integrated signal density corresponding to labeled antigen or antibody in each LN was calculated using LivingImage (PerkinElmer) or ImageJ software and plotted using GraphPad Prism 8 ...
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bioRxiv - Immunology 2023Quote: ... After secondary antibody incubation cells were directly imaged and analyzed using Operetta CLS High Content Analysis System (PerkinElmer).
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bioRxiv - Cell Biology 2020Quote: ... mice were anesthetized with 2% isoflurane and renilla bioluminescence was visualized/quantified by the IVIS Spectrum In Vivo Imaging System (PerkinElmer, 5-minute medium binning) in CDP-UMCG ...
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bioRxiv - Cell Biology 2020Quote: ... the mice were anesthetized with 2% isoflurane and firefly bioluminescence was visualized/quantified using the IVIS Spectrum In Vivo Imaging System (PerkinElmer, 5-minute medium binning) in CDP-UMCG ...
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bioRxiv - Immunology 2021Quote: ... Plasma 2-[1-14C]-Deoxy-D-glucose radioactivity was directly measured in a liquid scintillation counter (Tri-Carb 2810 TR, Perkin Elmer, Waltham, MA, USA). Tissue lysates were processed through ion exchange chromatography columns (Bio Rad Laboratories Inc. ...
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bioRxiv - Plant Biology 2020Quote: ... 5 to 10 mg powder were mixed with 2 ml scintillation cocktail and counts per minute (cpm) recorded with a TRI-Carb 2810TR liquid scintillation analyzer (Perkin Elmer, Waltham, MA, USA).
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bioRxiv - Biochemistry 2021Quote: ... Plates were incubated at room temperature for 2 h before measurement of the fluorescence using an EnVision Multimode Plate (Reader PerkinElmer Life and Analytical Sciences). All values were converted to cAMP concentration using cAMP standard curve performed parallel and data were subsequently normalized to the response of 100 μM forskolin in each cell line ...
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bioRxiv - Genomics 2023Quote: ... was measured in triplicate and averaged at 405 nm for 45-60 minutes at 25°C on a Wallac Victor 2 1420 Multilabel Counter Plate Reader (Perkin Elmer, Waltham, MA, USA). Citrate synthase activity was standardized against the protein concentration of each sample using the Bovine Serum Albumin Assay (500-0201 ...
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bioRxiv - Cancer Biology 2023Quote: ... mRNA was then extracted from 2 ug of total RNA per sample using NEXTFLEX® Poly(A) Beads 2.0 (Perkin Elmer, Austin, Texas, USA). Libraries were prepared from mRNA samples using NEXTFLEX® Rapid Directional RNA-seq Kit 2.0 (Perkin Elmer ...
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bioRxiv - Immunology 2020Quote: ... Streptavidin-coated Alpha donor beads or anti-His-conjugated AlphaLISA acceptor beads (PerkinElmer) were used at a final concentration of 10 μg/mL per well ...
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bioRxiv - Microbiology 2022Quote: ... Goat anti-rabit immunoglobulin G conjugated with horse-radish peroxidase (Perkin Elmer, USA) was used as secondary antibody ...
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bioRxiv - Developmental Biology 2023Quote: ... anti-DIG-POD signal was amplified with TSA Biotin System Kit (Perkin Elmer) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2023Quote: ... Goat anti-rabbit immunoglobulin G conjugated with horse-radish peroxidase (Perkin Elmer, USA) or goat anti-mouse immunoglobulin G conjugated with horse-radish peroxidase (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were washed again and incubated with the appropriate Opal tertiary antibody (1:100 in amplification diluent, Perkin Elmer) for 60 min ...
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bioRxiv - Genomics 2022Quote: SARS-CoV-2 genome libraries were constructed by using the NEXTFLEX® Variant-Seq™ SARS-CoV-2 kit with the automated high-throughput liquid handler Sciclone® G3 NGS (Perkin Elmer, USA) according to the manufacturer’s instructions.
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bioRxiv - Biophysics 2020Quote: ... and SYBR Gold to the specified dye concentrations (0.03 to 60 μM) and analyzed the sample in a 10 × 2 mm2 cuvette (Perkin Elmer UV/VIS Spectroscopy Cell B0631122) for 5 min for each condition ...
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bioRxiv - Neuroscience 2021Quote: ... Molecular weight bands were visualized by incubation with peroxidase-conjugated anti-mouse or anti-rabbit secondary antibody (1:5000; Jackson Immuno Research Laboratories) followed by treatment with the ECL-plus reagent (Western Lighting; PerkinElmer) and exposure on G:Box Chemi XRQ system (Syngene) ...
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bioRxiv - Neuroscience 2022Quote: ... membranes and immunoblots were carried out using primary antibodies (Table S4) followed by incubation with appropriate secondary horseradish peroxidase-conjugated antibodies (Table S5) and chemoluminiscent detection (Western Lightning, PerkinElmer). All antibodies were diluted in PBS containing 5% semi-skimmed milk and 0.1% Tween-20 ...
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bioRxiv - Microbiology 2024Quote: ... Blots were probed with the indicated antibodies and developed using the Western Lighting plus ECL (Perkin Elmer, Waltham, MA, USA). Blots were imaged on the Bio-Rad ChemiDoc MP Imaging System (BioRad ...
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bioRxiv - Immunology 2020Quote: ... Bound IgG was detected with Eu-N1 Anti-Human IgG (Perkin Elmer, 1244-330). using DELFIA-TRF system (Perkin Elmer).
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bioRxiv - Biochemistry 2022Quote: ... and lastly 20 μg mL−1 Anti-c-Myc AlphaLISA® Acceptor bead (PerkinElmer) completed the total reaction volume of 25 μL ...