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Citations for PerkinElmer :
501 - 550 of 728 citations for Locostatin CAS 90719 30 5 100% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The membranes were incubated with primary and secondary HRP-conjugated antibodies in PBST (5% milk and 0.1% Tween-20) to detect the protein of interest by Western Lightning Plus ECL (PerkinElmer) and ImageQuant LAS 4000 imaging system (GE) ...
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bioRxiv - Microbiology 2022Quote: ... A series of images spanning 5 µm were acquired with 0.2 µm spacing and images were deconvolved with VOLOCITY software (PerkinElmer) to report a single combined z-stack image.
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bioRxiv - Immunology 2022Quote: ... 5 μL of the IgG supernatants were transferred to an OptiPlate-384 assay plate (white opaque, PerkinElmer, Waltham, MA), mixed with 10μL (10 nM final conc. ...
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bioRxiv - Immunology 2021Quote: ... Immunohistochemistry was performed in Tris/NaCl blocking buffer (0.1□M Tris-HCl, 0.15□M NaCl, 5□μg/ml blocking reagent (Perkin Elmer), pH 7.5 ...
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Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. colibioRxiv - Microbiology 2020Quote: Autophosphorylation of YegI and point mutants was performed by addition of 5 μCi of [γ-32P] ATP (Perkin Elmer) to 0.2 μM of purified YegI or YegI point mutants in 10 μl of reaction buffer containing 50 mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... with a total length of 109 ntb was prepared by 5′-end-labelling using [γ-32P]- ATP (Perkin Elmer) and T4 polynucleotide kinase (Thermo Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... were collected from the CA1 SR from ≥5-7 sections per slice and processed for iterative deconvolution (99% confidence; Volocity 4.0, PerkinElmer). Three dimensional (3-D ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated in 24-well plates for 2 h with 0.25 ml/well of fresh growth medium containing 5 μCi/ml [3H]adenine (PerkinElmer). The medium was then replaced with 0.5 ml/well of DMEM containing 20 mM HEPES ...
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bioRxiv - Biochemistry 2024Quote: ... The dry filter papers were placed in scintillation vials with 5 ml Ultima Gold F scintillation cocktail (Perkin Elmer) and scintillation was measured in a TriCarb 2900TR liquid scintillation analyzer (Perkin Elmer).
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bioRxiv - Genomics 2023Quote: ... The metabolite extract was transferred into scintillation vials with 5 mL Insta-Gel Plus scintillation cocktail (Perkin Elmer #601339).
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bioRxiv - Cancer Biology 2022Quote: ... and maintained in 2.5 mL of KSFM supplemented with 5% (v/v) charcoal-stripped FBS and 50 nM R1881 (PerkinElmer) 25 ...
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bioRxiv - Immunology 2022Quote: ... 5% CO2 the luciferase activity was measured on Centro LB 960 Microplate Luminometer (Berthold Technologies) using Britelite PlusTM (PerkinElmer) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... oligonucleotides were 5’-end-labelled with [γ-32P]-ATP (10 mCi ml-1, 3000 Ci mmol-1, Perkin Elmer) with polynucleotide kinase (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... Purified RNA oligos were either phosphorylated with unlabeled ATP or 5’ end-labeled using [γ-32P] ATP (Perkin Elmer) and T4-Polynucleotide kinase (New England Biolabs) ...
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bioRxiv - Neuroscience 2024Quote: ... we administered four intraperitoneal injections of 3H-Thy at 2 hrs intervals to adult mice (1.67 μL/g body weight, specific activity 5 Ci/mmol; PerkinElmer) with subsequent perfusion 2 hrs after the last injection (n = 3) ...
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bioRxiv - Microbiology 2024Quote: Overnight cultures were diluted to an OD600 of ∼0.05 in 10 mL LB Miller broth supplemented with 5 µCi/mL 32P ortho-phosphoric acid (PerkinElmer) and grown until reaching an OD600 of ∼0.6 ...
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bioRxiv - Neuroscience 2024Quote: HB(T)MVECs were incubated for 6 hours in EGM-2 containing 0.4 μCi/ml [5-3H]-D-glucose (PerkinElmer). Supernatant was transferred into glass vials containing perchloric acid and sealed with rubber stoppers ...
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bioRxiv - Developmental Biology 2024Quote: ... The RNAscope Multiplex Fluorescent Detection Reagents v2 and the TSA Plus Cyanine 3 and 5 fluorophore (Perkin Elmer, NEL744001KT) were applied according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... The probes were visualized using Opal 520 and Opal 570 reagent (1:500 and 1:1000, respectively; PerkinElmer, Woodbridge, ON, CA). PHOX2B immunoreactivity was detected using mouse monoclonal PHOX2B antibody (B-11 ...
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bioRxiv - Immunology 2021Quote: ... 100 μl of Tropix Accelerator II was added and the luminescence was read in an Envision 2103 microplate luminometer (PerkinElmer). For β-Galactosidase detection with FDG ...
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bioRxiv - Evolutionary Biology 2020Quote: Bioluminescence was measured in 100 μL culture (or a 10-fold dilution series) in an Envision Multilabel Plate Reader (PerkinElmer). Clonal bioluminescence was also qualitatively assessed by examining colonies on LB agar in an AlphaImager HP light box (ProteinSimple ...
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bioRxiv - Microbiology 2020Quote: ... Sample well infection was normalized to aggregated DMSO plate control wells and expressed as Percentage of Control [POC = (%Infectionsample/ Average %InfectionDMSO)*100] and Z-score [Z= (%Infectionsample – Average %InfectionDMSO) / Standard Deviation %InfectionDMSO]in Spotfire (PerkinElmer). Candidate hits were selected as compounds with POC<40% and viability >80% ...
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bioRxiv - Neuroscience 2022Quote: ... 2.7 mM KCl with 0.2% Triton X-100 and then blocked by shaking for 60 min in TSA Blocking Reagent (Perkin Elmer). Sections were left shaking for 1-3 nights at 4°C in primary antibodies ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were washed in PBSTw and then incubated in FITC-Tyramide (Perkin-Elmer) 1:100 in amplification reagent (Perkin Elmer), followed by several washes in PBSTw ...
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bioRxiv - Neuroscience 2021Quote: ... during 2 hours at RT followed by a 10 minutes incubation at RT with TSA Biotin System (Biotin TSA 1:100, NEL700A001KT PerkinElmer). After several washes ...
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bioRxiv - Neuroscience 2021Quote: ... The signal of CB1 receptor hybridization was revealed by a TSA reaction using cyanine 3 (Cy3)-labeled tyramide (1:100 for 10 minutes, NEL744001KT PerkinElmer). After several washes ...
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bioRxiv - Microbiology 2021Quote: Fourier-transform Infrared (FTIR) spectra of the nanocomposites were documented on FT-IR Spectrometer (Perkin Elmer-Spectrum 100 Optica, USA) to study the functional group present in the system ...
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Oxytocin receptor activation does not mediate associative fear deficits in a Williams Syndrome modelbioRxiv - Animal Behavior and Cognition 2021Quote: ... 100 nM GBR12909 (to block binding to dopamine transporter) and 50 pM [125I]RTI-55 (NEX272, Perkin Elmer, Boston, MA) were added to the buffer ...
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bioRxiv - Microbiology 2020Quote: ... Uptake was measured at room temperature by mixing 100 µl of cells with 100 µl of transport buffer containing 8 µM of [14C] glycerol (0.125 µCi each in triplicates, PerkinElmer Inc.). Incorporation of radioactivity was determined as described above.
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bioRxiv - Cancer Biology 2023Quote: ... To generate orthotopic lung tumors Keap1 WT or Keap1 R470C KP cells expressing luciferase GFP were injected intravenously (100,000 cells in 100 uL PBS) into female C57BL/6J (JAX strain #000664) mice and tumor burden was measured by bioluminescence (PerkinElmer IVIS Spectrum In Vivo Imaging System ...
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bioRxiv - Microbiology 2023Quote: Brine samples BP3 and BP4 were spiked with L-[4,5-3H] leucine (Perkin Elmer, specific activity 100 Ci mmol-1) at a final concentration of 20 nmol leucine L-1 (Kirchman et al. ...
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bioRxiv - Neuroscience 2024Quote: ... 100 µL of cell homogenate (0.05 mg protein/well) and 100 µL of [3H]8-OH-DPAT (0.5 nM final concentration, 170 Ci/mmol, Perkin Elmer) in a final volume of 1 mL ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were labeled for 15 min at 37 °C with 100 µCi of 35S-methionine (PerkinElmer Life Sciences, Boston, MA). After incubation ...
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bioRxiv - Biophysics 2023Quote: ... Fluorescence was collected after a pinhole of 100 μm diameter size (P100D, Thorlabs) by an avalanche photodetector (SPCM-AQR-15, PerkinElmer) connected to an SPC-150 (Becker & Hickl ...
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bioRxiv - Cell Biology 2023Quote: ... media was replaced with fresh media containing 200 μM ascorbate and 100 mCi/mL of 35S-Cys/Met (Perkin Elmer) for 10 min ...
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bioRxiv - Immunology 2023Quote: ... Virus–monoclonal antibody-containing medium was then aspirated from cells and 50 or 100 µl of a 1:2 dilution of SteadyLite Plus (PerkinElmer) or BioGlo (Promega ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 5E4 cells per well were seeded in 100 µL of growth medium into poly-D-lysine-coated white 96 well OptiPlates (PerkinElmer). The following day ...
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bioRxiv - Immunology 2024Quote: ... target cells were labelled with 50-100 µCi 51Cr radionuclide as sodium chromate in normal saline (PerkinElmer, Waltham, MA, USA) and incubated at 37 °C ...
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bioRxiv - Neuroscience 2024Quote: Free-floating sections were rinsed 3 times for 2 min each in 0.01 M PBS with 0.2% Triton X-100 and then were blocked for 20 min in TSA Blocking Reagent (Perkin Elmer). Sections were incubated with primary antibodies (Supplementary Table 2 ...
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bioRxiv - Pathology 2024Quote: To quantify calcification status of the samples sections were incubated in phosphate buffered saline containing 100 pmol/ml Osteosense solution (IVI SenseOsteo 680 #NEV10020Ex, PerkinElmer) for 24h at 37 °C in the dark ...
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bioRxiv - Cancer Biology 2021Quote: In vitro methyltransferase assays were performed using 30 ul reaction buffer (50 mM Tris-HCl pH 7.8, 50 mM KCl, 5 mM MgCl2) with 1 ul 3H-AdoMet (Perkin Elmer, specifific activity = 55-85 Ci/mMole,0.55 μCi/ul ...
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bioRxiv - Cell Biology 2020Quote: ... and labeled for 5 min with with 1 μL per OD of cells of EXPRESS 35S Protein Labeling Mix (PerkinElmer) for 5 or 10 min ...
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bioRxiv - Microbiology 2020Quote: ... the plates containing infected macrophages were incubated at 37°C with 5% CO2 for 18 h in an Operetta High-Content Imaging System (PerkinElmer). During this 18 h incubation ...
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bioRxiv - Developmental Biology 2021Quote: ... the cells were incubated for 5 minutes at 37°C with 55µCi/well of 35S-L-methionine and 35S-L-cysteine Promix (Perkin Elmer) in a minimal volume of culture medium ...
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bioRxiv - Cell Biology 2022Quote: ... cell motility was acquired using temperature (37°C) and CO2 (5%) controlled Operetta CLS High-Content Analysis System (PerkinElmer, HH16000000) (non-confocal ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Spectra were analysed using OPUS 6.5 software (Bruker) and compared with RMIT University’s spectral library of organic compounds generated using Spectrum 10 software (Perkin Elmer). Fingerprint spectra shown in Figure 4D were prepared using GraphPad Prism.
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bioRxiv - Microbiology 2021Quote: ... 1.37 μg/mL of RBD and 5 μg/mL of Ecto) and incubated overnight in protein-binding 96-well plates (PerkinElmer). The next day ...
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bioRxiv - Neuroscience 2021Quote: ... plates were centrifuged once more under same conditions mentioned above and 5 µL of each FRET antibody pair was added (2.5 nM final concentration for donor and 5 nM for acceptor, diluted in 1x Lance buffer, (Perkin Elmer)) ...
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bioRxiv - Neuroscience 2020Quote: ... In situ hybridization using nitro-blue tetrazolium and 5-bromo-4-chloro-3′-indolyphosphate and double color in situ hybridization using TSA Plus (PerkinElmer) were performed as previously described (Yamagata et al.,1999 ...
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bioRxiv - Microbiology 2021Quote: ... and fluorescence at 355 nm excitation and 460 nm emission was monitored every 5 minutes for 50 minutes using an Envision plate reader (PerkinElmer). Rate of substrate cleavage was determined using linear regression of the raw data values obtained during the time course ...