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Citations for PerkinElmer :
501 - 550 of 696 citations for 2 Methyl 3 4 sulfobutyl benzoxazolium betaine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Transcription was initiated with addition of 12 pmol rrnBP1 PCR promoter fragment pre-mixed with 1 mM ATP and 4 μCi α-[32P]-CTP (3,000 Ci mmol−1; Perkin Elmer) at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... at 42 °C for 30 min followed by hybridization overnight at 42°C with 4 pmol probes (Table S3) that were radiolabeled using [γ-32P] ATP (PerkinElmer) and T4 Polynucleotide kinase (New England Biolabs) ...
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bioRxiv - Cancer Biology 2023Quote: ... Following an HRP conjugation step a TSA Opal 570 1:150 (Tyramide Signal Amplification Opal 4-Color Manual IHC Kit, Perkin Elmer) was linked to the IRF7 antibody previously bound to the slide overnight ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cells were incubated for four hours at 4 °C in the presence of benzazepine-red nonpeptide vasopressin antagonist (BZ-DY647, PerkinElmer Revvity) at various concentrations ranging from 10-10 to 10-7 M ...
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bioRxiv - Molecular Biology 2024Quote: ... and were then anesthetized (isoflurane 4 % for induction and 1.5 % for maintenance) before placing in the optical imaging system (IVIS Lumina III, PerkinElmer, Courtaboeuf, France). In vivo bioluminescence signals were measured in selected regions of interest (injected testes ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated for 10 min in Cy3 Plus Amplification Reagent diluted 1:300 in 1x Plus Amplification Diluent (TSA Plus Cyanine 3 System, Perkin Elmer, Waltham, MA, NEL744001KT), washed several times in TBS with 0.3% Triton X-100 and 0.05% Tween 20 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Ligand-stimulated cAMP accumulation in the presence or absence of 0.5 mM 3-isobutyl-1-methylxanthine (IBMX) was measured after the indicated times of stimulation using LANCE® cAMP Detection Kit (Perkin Elmer Life Sciences) and a Mithras LB 940 multimode microplate reader ...
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bioRxiv - Biophysics 2021Quote: ... emission 535 nm) measured for 1 hour at 2 minute intervals on an EnVision Xcite 2104 (PerkinElmer). Enzymatic activity of each mutant was measured with (+ Palm-CoA ...
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bioRxiv - Systems Biology 2021Quote: ... columns 2-23 were used) of tissue-culture treated black CellCarrier-384 Ultra Microplates (Perkin Elmer 6057302) using a 12-channel electronic multichannel 200 μL pipettor [Sartorius] ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM MnCl2) supplemented with 10 μM ATP and 2 μCi γ-32P-ATP (blu002z250uc, Perkin Elmer) in 15μL reactions ...
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bioRxiv - Cell Biology 2020Quote: ... and the wells were covered immediately using filter papers soaked with hyamine hydroxide (Perkin Elmer, 2-19361). After incubation in a fume hood for at least 12 hours to reach saturation ...
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bioRxiv - Cell Biology 2020Quote: ... and the wells were covered immediately using filter papers soaked with hyamine hydroxide (Perkin Elmer, 2-19361). After incubation in a fume hood for at least 12 hours to reach saturation ...
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bioRxiv - Developmental Biology 2023Quote: ... Anesthetized (2% isoflurane vaporized in 1 L/min O2) dams were imaged (IVIS Lumina III, Perkin Elmer) with their ventral side facing the charge-coupled device camera (15 cm field of view ...
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bioRxiv - Cell Biology 2023Quote: ... cells on the SPA plate were suspended in 1 mL of DDW with 2% glusulase (PerkinElmer, #NEE154001EA) and digested with shaking at room temperature for 3 hr ...
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bioRxiv - Immunology 2022Quote: ... Uptake was initiated by addition of 1 μCi [3H]2-DG (25-30 Ci/mmol, PerkinElmer, NET549) and 0.1 mM unlabeled 2-DG (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were loaded with 2 μCi/ml D-[2,3-3H]aspartate (Perkin Elmer, Waltham, MA, cat. #NET50100) in MEM +HIHS cell culture medium ...
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bioRxiv - Plant Biology 2023Quote: ... resuspended in 2 N NaOH and mixed with 5 volumes scintillation fluid (Ultima Gold™ XR, PerkinElmer) for 14C-CPM counting in a TRI-CARB liquid scintillation counter (PerkinElmer ...
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bioRxiv - Neuroscience 2023Quote: ... 2 µL of cell culture medium or standard solution was added to an Optiplate-384 microplate (PerkinElmer). 21µL of 10X mixture including acceptor beads and biotinylated antibody was then added to the wells with culture media or standard solution ...
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bioRxiv - Microbiology 2024Quote: ... and the luminescent signal was measured with an IVIS Lumina II (Perkin Elmer; 2-min exposure time). The luminescent signal was quantified with Living Image software (Perkin Elmer ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Radiolabeled [U-14C] palmitic and [2-14C] acetic acids were purchased from PerkinElmer (Villebon-Sur-Yvette, France). RIPA buffer 10X (9806-Cell Signaling ...
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bioRxiv - Developmental Biology 2021Quote: ... where reactions were incubated with 150 nM of the gel-filtration purified WT mouse Naa10 or Naa12 in a 30 µl reaction volume containing each 250 µM substrate peptide and radiolabeled [14C]acetyl-CoA (4 mCi/mmol; PerkinElmer Life Sciences) for 12 min (Naa12 ...
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bioRxiv - Microbiology 2022Quote: ... were performed in a HORIBA JOBIN YVON Spectrofluorometer (FluoroMax – 4) (HORIBA Scientific, Edison, New Jersey, USA) in a fluorescence cuvette of path length 5 mm (Perkin Elmer B0631123), after putting 0.5ml of the sample solution in it ...
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bioRxiv - Biophysics 2021Quote: ... 50 nM SpNatC (WT or mutants) was mixed with 50 μM Ac-CoA (14C-labeled, 4 mCi mmol-1; PerkinElmer Life Sciences) and varying peptide concentrations (ranging from 1.95 μM to 500 μM ...
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bioRxiv - Neuroscience 2023Quote: ... Half of the cell Triton X lysate from each well was pipetted into a scintillation vial and 4 ml of scintillation fluid (#6013329, Ultima GoldTM, Perkin Elmer, USA) was added ...
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bioRxiv - Molecular Biology 2023Quote: ... were prepared in in vitro transcription reaction on linear PCR-generated template containing RNAImod (4) with 500 μM ATP or NAD and 25 μM [α 32P] CTP (Perkin Elmer) using hexa-histidine tagged E ...
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bioRxiv - Microbiology 2024Quote: ... Mixtures were incubated for 60 min at room temperature prior to adding 4 µl of D-luciferin (30 mg/ml) (Perkin Elmer; #122799). After additional incubation for 5 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Unbound [111In]In-DOTA-hG250 was removed and the cell-associated activity was measured in a γ-counter (1480 Wizard 3; LKB/Wallace, Perkin Elmer, Boston, MA, USA).
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bioRxiv - Neuroscience 2020Quote: ... animals were anesthetized (2 % isofluorane gas in O2) and luminescence was recorded (IVIS Spectrum, Perkin Elmer, Waltham, MA). Images were analyzed with Living Image Software (Caliper LifeSciences).
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bioRxiv - Plant Biology 2020Quote: ... N was quantified in approximately 2 mg subsamples using a CHN elemental analyzer (EA2400, PerkinElmer, Waltham, MA, USA). For phosphorus content ...
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bioRxiv - Neuroscience 2020Quote: HUVECs were incubated for 6 hours in EGM-2 containing 0.4 μCi/ml [5-3H]-D-glucose (PerkinElmer). Supernatant was transferred into glass vials containing perchloric acid and sealed with rubber stoppers ...
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bioRxiv - Molecular Biology 2020Quote: ... In brief, using a rabbit antimelatonin antiserum (PF1288; P.A.R.I.S., Paris, France) and 2-Iodomelatonin (NEX236050UC; PerkinElmer, Boston, Massachusetts) as tracer RIA was performed ...
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bioRxiv - Microbiology 2024Quote: ... 200 μl of each fraction was added to 2 ml of Ultima Gold liquid scintillation cocktail (Perkin Elmer), and the radioactivity was measured by a LS-6500 scintillation counter (Beckman) ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed twice for 2 minutes with wash buffer and incubated in Opal 520 (Perkin Elmer FP1487A) for 30 minutes at 40°C ...
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bioRxiv - Bioengineering 2022Quote: ... mice were shaved then anesthetized with 1–2% isoflurane using a calibrated vaporizer inside an IVIS Spectrum (PerkinElmer). Non-invasive longitudinal monitoring of tumour progression and nanoparticle release was followed by scanning mice with the IVIS Spectrum-bioluminescent and fluorescent imaging system (PerkinElmer) ...
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bioRxiv - Biophysics 2022Quote: ... and incubated at 40 C for 2 h before counting with a MicroBeta scintillation top counter (Perkin Elmer).
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed twice for 2 minutes with wash buffer and incubated in Opal 520 (Perkin Elmer FP1487A) or vivid 520 (ACDbio 323271 ...
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bioRxiv - Biochemistry 2023Quote: ... the beads were suspended in 25 µl of cytoplasmic buffer containing 2 µCi of 3H-labeled SAM (PerkinElmer) and incubated overnight at 32 °C without shaking ...
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bioRxiv - Molecular Biology 2024Quote: ... cAMP- dependent luminescence was then measured at 2-min intervals using a PerkinElmer Envision 2104 plate reader (PerkinElmer), as described [24] ...
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bioRxiv - Physiology 2020Quote: ... Progesterone was extracted with ethyl ether after overnight equilibration (4°C) of the plasma with 1500 dpm of tritiated progesterone (Perkin Elmer, Rodgau, Ermany). Separation of the organic and aqueous phase was conducted similar to the procedure described for the other hormones ...
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bioRxiv - Biochemistry 2021Quote: ... coli and all constructs were adjusted to approximately 4 μM concentration in cell lysate soluble fractions using a microcapillary electrophoretic system (Caliper; PerkinElmer, Waltham, MA, USA) and frozen in 96-well plates.
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bioRxiv - Genetics 2022Quote: ... and the final spectrophotometric reading was carried out by microplate reader (Perkin Elmer Wallac 1420 Victor 2 Multi-Label) set at a wavelength of 450 nm.
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bioRxiv - Neuroscience 2020Quote: ... or a linear OXTR antagonist [125I]-d(CH2)5[Tyr(Me)2-Tyr-Nh2]9-OVT (Perkin Elmer, USA) as tracers ...
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bioRxiv - Biochemistry 2021Quote: The following drugs were used in this study: R1881 (methyltrienolone; used at 2 nM) (PerkinElmer, Inc., Waltham, MA, USA) and hydroxyflutamide (HO-flutamide ...
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bioRxiv - Microbiology 2021Quote: ... 100µL of undiluted PVs were serially diluted 2-fold down a white 96-well F-bottom plates (Perkin Elmer) in 50µL of DMEM ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... coelenterazine 400a was added to a final concentration of 2.5 μM and 2 min later BRET was measured on an EnVision 2104 Multilabel Reader (PerkinElmer) using 410/80 nm (donor ...
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bioRxiv - Microbiology 2020Quote: ... 293FT cells (2 × 104 cells) were plated in 96-well View Plates (Perkin Elmer Life Sciences, Waltham, MA, USA). Five hours later ...
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Secretin receptor as a target in gastrointestinal cancer: expression analysis and ligand developmentbioRxiv - Cancer Biology 2022Quote: ... to a concentration of 200 μM with an addition of 0.5 mCi carrier-free 125I-NaI in 2 μl of carrier solution (PerkinElmer). Iodination was initiated by addition of 4 μl chloramin-T solution (SigmaAldrich ...
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bioRxiv - Immunology 2021Quote: ... The SARS-CoV-2 spike protein antigen (2.0µg/mL) was directly adsorbed on half-area 96-well OptiPlate (96W HB, Perkin Elmer) microplates for 2h at 37°C in a humidified incubator.
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bioRxiv - Cell Biology 2020Quote: ... placed in polistor tubes with 2 ml of Ultima gold™XR liquid scintillation cocktail (Perkin Elmer, Massachusetts, USA) and counted in a scintillation counter ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were split on day 2 and 10,000 cells were seeded in each well (96 well-plate; CellCarrier-96 Ultra Microplates, Perkinelmer). At 12-14 h after cell seeding ...