Labshake search
Citations for PerkinElmer :
451 - 500 of 1011 citations for WesternBright MCF Fluorescent Western Blot Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... Tyramide signal amplification reaction was performed using Cy3 fluorescent dye (diluted 1:50 using amplification diluent) (Perkin Elmer Life Sciences, Inc., NEL753001kt). The embryos were co-stained with CrebA and Crb antibodies for visualizing SG nuclei and apical cell boundaries ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... each mouse was injected retroorbitally with 80 μl of solution containing a 1:1 mixture of MMPSense 750 FAST Fluorescent Imaging Agent (MMP750) (PerkinElmer, Waltham, MA) and monoclonal anti-type II collagen antibody that had been coupled with 680 dye (MabCII680 ...
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bioRxiv - Physiology 2023Quote: ... Elastase activity measurements in whole aorta tissue or tissue sections were performed using the far-red fluorescent elastase substrate Neutrophil Elastase 680 FAST probe (NE680; Perkin Elmer # NEV11169). Immunofluorescent detection of neutrophil elastase was performed on aortic sections from apoE-/- mice (The Jackson Laboratory strain # 002052) ...
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bioRxiv - Immunology 2023Quote: ... Hybridization signals were detected by fluorescent signal using peroxidase-based Tyramide Signal Amplification-Plus Fluorescein or TSA Plus Cyanine 5 (Perkinelmer, Shelton, CT). Finally ...
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bioRxiv - Neuroscience 2023Quote: ... brains were incubated during 2 weeks at 37°C in presence of an AT8 antibody that specifically binds hyperphosphorylated tau (pSer202/Thr205/PSer208, produced at Janssen Pharmaceutica) and is directly labelled with a near-infrared fluorescent tag (PerkinElmer VivoTag 680XL). Brain samples were then dehydrated ...
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bioRxiv - Biophysics 2024Quote: ... samples were mounted upside down and cilia were live-stained with fluorescent-dye conjugated tomato lectin (IVISense™ Tomato Lectin 680, Perkin Elmer) by incubating the sample in a 0.25 µM dilution in PBS for 20 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... The bound primary antibodies were detected by horseradish peroxidase-conjugated secondary antibodies in combination with an enhanced chemiluminescence detection system using Western Lightning Chemiluminescence Reagent (PerkinElmer Life Sciences) or ImmunoStar (Wako Pure Chemical Industries) ...
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bioRxiv - Cell Biology 2021Quote: ... Horseradish peroxidase (HRP) conjugated secondary antibody was added in order for labelled proteins to be detected by Western Lightning Plus-ECL (PerkinElmer, 203-170071). Primary and secondary antibodies used are listed in Supplementary Table 5.
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bioRxiv - Immunology 2021Quote: ... HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning oxidizing and luminol reagents (PerkinElmer Life Sciences). Light emission was measured with an LB942 TriStar luminometer (Berthold Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning oxidizing and luminol reagents (PerkinElmer Life Sciences). Light emission was measured with an LB 942 TriStar luminometer (Berthold Technologies) ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed at room temperature 3 times 10 min in PBST before using chemiluminescence to reveal protein bands with the Western Lightning® Plus-ECL Enhanced Chemiluminescence reagent (PerkinElmer Inc.) as per the manufacturer’s instructions.
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bioRxiv - Physiology 2021Quote: ... This enabled detection of the CASR at the appropriate molecular weight after incubation with Western Lightning Plus ECL reagents (PerkinElmer, Boston, MA) and visualized using a Kodak Image Station 440CF (Kodak ...
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bioRxiv - Molecular Biology 2021Quote: ... HRP-conjugated secondary antibodies were diluted 1:5000 in TBS-T with 5% milk and incubated with the membrane at room temperature for an hour before performing the chemiluminescence reaction (Western Lightning Plus-ECL, Enhanced Chemiluminescence Substrate, Perkin Elmer NEL105001EA). Signal was captured with either X-ray films (CL-XPosure™ Film ...
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bioRxiv - Cell Biology 2022Quote: ... Washes were performed with 0.1% Tween-20 tris buffered saline and blots developed using ECL chemiluminescence (Perkin Elmer #NEL105001EA or Cytiva #RPN2232) and autoradiography films (Thomas Scientific #1141J52).
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bioRxiv - Microbiology 2021Quote: ... The mixture was incubated at 37 °C for 20 min before the RFU of fluorescent signals were read on an Envision microplate reader (Perkin Elmer, Waltham, MA) with excitation wavelength set at 320 nm and emission wavelength set at 400 nm ...
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bioRxiv - Microbiology 2021Quote: ... SVC1-scramble and isogenic non-invasive SVC1 bacteria (lacking pSiVEC-scramble) were fluorescently labeled using the XenoLight RediJect 750 near-infrared fluorescent probe (Perkin Elmer, Waltham, MA). Nine-week-old female BALB/c mice (Jackson Laboratory ...
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bioRxiv - Bioengineering 2023Quote: ... Cardiomyocyte area and cardiomyocyte number were quantified by identifying cell membranes through staining tissue samples with fluorescent-tagged wheat germ agglutinin (WGA) and quantified using Mantra™ quantitative pathology workstation (PerKinElmer, Waltham, MA)17 ...
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bioRxiv - Genomics 2019Quote: ... Anti-rabbit or anti-mouse HRP conjugated antibodies were used to detect protein by chemiluminescence using ECL (Perkin Elmer, Western Lightning Plus ECL). Images were visualized by GeneSnap image analysis software (SynGene ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Bound antibodies were detected using horseradish peroxidase-conjugated secondary antibodies and visualized by chemiluminescence using Western Lightning Plus-ECL reagent (Perkin Elmer, United States). Band intensities were analysed using ImageJ (National Institutes of Health ...
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bioRxiv - Microbiology 2021Quote: ... HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning oxidizing and luminol reagents (Perkin Elmer Life Sciences). Light emission was measured with a LB942 TriStar luminomete (Berthold Technologies) ...
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bioRxiv - Microbiology 2020Quote: ... HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning oxidizing and luminol reagents (Perkin Elmer Life Sciences). Light emission was measured with a LB941 TriStar luminometer (Berthold Technologies) ...
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bioRxiv - Immunology 2021Quote: ... HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning oxidizing and luminol reagents (Perkin Elmer Life Sciences). Light emission was measured with a LB941 TriStar luminometer (Berthold Technologies) ...
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bioRxiv - Immunology 2021Quote: ... HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning oxidizing and luminol reagents (Perkin Elmer Life Sciences). Light emission was measured with a LB942 TriStar luminometer (Berthold Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning oxidizing and luminol reagents (Perkin Elmer Life Sciences). Light emission was measured with a LB942 TriStar luminometer (Berthold Technologies) ...
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bioRxiv - Molecular Biology 2021Quote: ... HRP enzyme activity was determined after addition of 35 µl per well of a 1:1 mix of Western Lightning oxidizing and luminal reagents (Perkin Elmer Life Sciences) supplemented with 150 mM NaCl ...
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bioRxiv - Immunology 2021Quote: ... HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning oxidizing and luminol reagents (Perkin Elmer Life Sciences). Light emission was measured with a LB941 TriStar luminometer (Berthold Technologies) ...
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bioRxiv - Immunology 2021Quote: ... HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning oxidizing and luminol reagents (Perkin Elmer Life Sciences). Light emission was measured with a LB941 TriStar luminometer (Berthold Technologies) ...
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bioRxiv - Microbiology 2022Quote: ... HRP enzyme activity was determined after the addition of 40 μL of a 1:1 mix of Western Lightning oxidizing and luminol reagents (Perkin Elmer Life Sciences). Light emission was measured with a LB942 TriStar luminometer (Berthold Technologies) ...
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were then washed twice with PBS/0.05%Tween and once with PBS and finally the signals were detected using Western Lightening chemiluminescent substrate (Perkin Elmer, Netherlands, Cat #NEL104001EA).
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bioRxiv - Neuroscience 2020Quote: ... and the fluorescent emission was read at 665 nm and 620 nm in an EnVision® Xcite Multilabel Plate Reader (PerkinElmer, Waltham, MA, USA). IP1 content was calculated based on a standard curve covering concentrations from 2.7–11,000 nM according to the IP-ONE kit protocol.
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bioRxiv - Pathology 2024Quote: The arterial retention of lipoproteins was quantified by a previously established method that measures the total fluorescent intensity obtained by confocal microscopy of at least five sections per carotid (Olympus IX-8 Perkin Elmer spinning disk microscope) (14–16) ...
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bioRxiv - Cancer Biology 2020Quote: ... The membranes were incubated with horseradish peroxidase-conjugated IgG as a secondary antibody and the Western Lightning®-ECL system (PerkinElmer Inc., Waltham, MA) for detection.
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bioRxiv - Biochemistry 2019Quote: ... Visualization of EOI was performed with enhanced chemiluminescent reagent (Pierce ECL Western Blotting Substrate) using an IVIS®Spectrum imaging system (Perkin Elmer, Hopkinton, MA) with blocked excitation filter and opened emission filter ...
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bioRxiv - Cancer Biology 2022Quote: ... filters were incubated for 1 hour at room temperature with alkaline phosphatase-coupled goat antirabbit antibodies and developed with Western Lightning Plus (Perkin Elmer, Inc., Waltham, MA) according to manufacturer directions.
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bioRxiv - Immunology 2021Quote: ... HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning ECL reagents (Perkin Elmer Life Sciences, Waltham, MA, USA). Light emission was measured with an LB 941 TriStar luminometer (Berthold Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... Chemiluminescence was determined in a Fusion® SL chemiluminescence reader (Vilber Lourmat Deutschland GmbH, Eberhardzell, Germany) from membranes incubated in Western Lightening® Plus-ECL solution (PerkinElmer, Waltham, MA, USA).
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bioRxiv - Microbiology 2024Quote: ... HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning oxidizing and luminol reagents (PerkinElmer Life Sciences, Waltham, MA, USA), quantification of bands was performed using the ImageLab software (Bio-Rad).
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Tumour Extracellular Vesicles Induce Neutrophil Extracellular Traps To Promote Lymph Node MetastasisbioRxiv - Cancer Biology 2023Quote: ... OPAL kit (PerkinElmer) fluorophores were used and prepared according to the manufacturer’s protocols ...
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bioRxiv - Genetics 2023Quote: ... DNA was extracted from oral mucosal tissue using a commercial kit (chemagic™ DNA Buccal Swab Kit, PerkinElmer and DNAdvance Kit, Beckman Coulter). We performed real-time PCR to determine genotypes of DM-associated mutations (SOD1:c.118G
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bioRxiv - Cell Biology 2019Quote: ... Fluorescein Evaluation Kit System (PerkinElmer) were used according to manufactures’ protocols (Wang et al. ...
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bioRxiv - Physiology 2020Quote: ... Fluorescein Evaluation Kit System (PerkinElmer) were used according to manufactures’ protocols(Wang et al. ...
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bioRxiv - Biochemistry 2022Quote: ... (PerkinElmer AlphaScreen Histidine Detection Kit) were added under low light conditions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and TSA kit (Perkin Elmer) for fluorescent visualization.
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bioRxiv - Neuroscience 2022Quote: ... or ATPlite kit (PerkinElmer, 6016941) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... Enhanced Chemiluminescence Detection Kit (PerkinElmer).
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bioRxiv - Immunology 2023Quote: ... Enhanced Chemiluminescence Detection Kit (PerkinElmer).
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bioRxiv - Immunology 2023Quote: ... Enhanced Chemiluminescence Detection Kit (PerkinElmer).
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bioRxiv - Biochemistry 2023Quote: ... AlphaScreen Histidine detection kit (PerkinElmer) consisting of streptavidin donor beads and nickle chelate (Ni-NTA ...
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bioRxiv - Immunology 2023Quote: ... Enhanced Chemiluminescence Detection Kit (PerkinElmer).
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bioRxiv - Cell Biology 2023Quote: ... Enhanced Chemiluminescence Substrate kit (PerkinElmer). Images were acquired using the iBright FL1000 imaging system (Thermo Fisher Scientific).