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Citations for PerkinElmer :
451 - 500 of 525 citations for Inactive tyrosine protein kinase 7 PTK7 Antibody HRP since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Endogenous peroxidase was then blocked by incubation in Antibody Diluent/Block (PerkinElmer #72424205, Massachusetts, USA) for 10 minutes ...
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bioRxiv - Microbiology 2020Quote: ... and the bound antibodies were detected using enhanced chemiluminescence solution (Perkin Elmer, Waltham, MA, USA). Images were acquired using the ChemoCam 6.0 ECL system (INTAS Science Imaging ...
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bioRxiv - Microbiology 2022Quote: ... Horseradish peroxidase-conjugated goat anti-rabbit polyclonal and anti-mouse antibodies were purchased from PerkinElmer Life Sciences (Boston ...
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bioRxiv - Cancer Biology 2020Quote: ... and bound primary antibodies were visualized using Western Lightning Plus-ECL (PerkinElmer Inc., Waltham, MA). The primary antibodies used in this study were as follows ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were pulse labeled for 1 hr with 120 μCi of EasyTag Express35S Protein Labeling Mix [35S]-Cys/Met (PerkinElmer; Waltham, MA). Immediately after the radioactive pulse ...
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bioRxiv - Cancer Biology 2020Quote: ... proteins were first labeled for 48 h with 0.25 μM Ci ml-1 L-[U-14C] VALINE (Perkin Elmer, Waltham, MA, USA) in GIBCO-RPMI 1640 medium (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... The precipitates proteins were resolved by SDS-PAGE and [32P]-labeled proteins was visualized using a Super Resolution screen (Perkin Elmer and quantified using a Cyclone Plus Storage Phosphor System (Perkin Elmer). The signal was normalized to the relative cellular content as determined by immunoblotting of whole cell lysates probed with antibodies.
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bioRxiv - Cell Biology 2022Quote: ... HEK293T cells transfected with FTTTRA25T and were labeled for 30 min with EasyTag Express [35S] Protein Labeling Mix (0.1 mCi/mL; Perkin Elmer CAT# NEG772014MC) in DMEM lacking both Cys and Met (Gibco CAT# 21013024 ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were finally washed three times with PBST for 5 min and protein levels were visualized by enhanced chemiluminescence (Perkin Elmer, NEL104001EA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed at room temperature 3 times 10 min in PBST before using chemiluminescence to reveal protein bands with the Western Lightning® Plus-ECL Enhanced Chemiluminescence reagent (PerkinElmer Inc.) as per the manufacturer’s instructions.
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bioRxiv - Biophysics 2020Quote: ... HEK cell membrane proteins (100 μg/ml final concentration) were incubated with 3 nM [3H]muscimol (30 Ci/mmol; PerkinElmer Life Sciences), neurosteroid (3 nM–30 μM ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2.3±0.64 pmol/mg protein (Bmax for β 2AR) and binding affinities for the radioligand [³H]CGP12,177 (specific activity 51 Ci/mmol, PerkinElmer, Rodgau, Germany) of 0.070 nM (KD for β1AR ...
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bioRxiv - Molecular Biology 2024Quote: ... 6 ng/ µl recombinant TopBP1 protein or buffer were added to TopBP1 immunodepleted interphase Xenopus egg extract containing 50 nCi/µl α32P-dCTP (Perkin Elmer NEG513A250UC). To start the replication reaction the egg extracts were supplemented with 5 ng sperm DNA (kindly provided by the lab of O ...
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bioRxiv - Cell Biology 2024Quote: Confluent 6-well dishes of FRT cells (approximately 1×106/well) were metabolically labeled in DMEM depleted of methionine and cysteine and supplemented with EasyTag 35S Protein Labeling Mix (Perkin Elmer, NEG772007MC), glutamine ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Biotinylated human recombinant KRAS WT, G12C, G12D, and G12V proteins (Amid Bioscience, Santa Clara, CA) were pretreated with Terbium-labeled streptavidin (Tb-SA) (PerkinElmer, Waltham, MA) and BODIPY-FL-GDP (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Cells were then starved in Met/Cys-free medium at 37°C for 30 minutes with dialyzed FBS before replacing the medium with 35S-Cys/Met enriched medium (11 µCi/µL, EasyTag™ EXPRESS35S Protein Labeling Mix, Perkin Elmer) for 5 hours at 37°C ...
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bioRxiv - Bioengineering 2024Quote: Capillary electrophoresis sodium dodecyl sulfate (CE-SDS) was performed on a LabChip GX II instrument using Protein Express 200 (Perkin Elmer, #760499) and Protein Express Assay Reagent Kit (Perkin Elmer ...
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bioRxiv - Cancer Biology 2024Quote: ... Equal amounts of cell lysate proteins (20-50 μg) or proteins eluted from agarose beads were separated on SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membranes (PerkinElmer Life Sciences). Membranes were blocked 1 h with 3% BSA/TBST and incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... Ten μl of these dilutions or 10 μl of G protein storage buffer without nucleotides were pipetted into the corner of a white opaque 96-well plate (Opti-Plate, PerkinElmer Life Sciences, 6005290). Ten 10 μl of 0.1 mM Coelenterezine 400a diluted in BRET buffer (140 mM NaCl ...
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bioRxiv - Developmental Biology 2021Quote: ... RRID: AB_2340590) were used as secondary antibody and blots were detected using ECL detection (PerkinElmer (PE), NEL105001EA) ...
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bioRxiv - Neuroscience 2020Quote: ... POD-conjugated antibody labeling of mRNA was proceeded with TSA Plus DNP signal amplification (Perkin Elmer) and a subsequently labeling with anti-DNP-KLH-488 antibodies (1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... Then those sections were stained with anti-MYC antibody with TSA plus fluorescein system (Perkin Elmer) followed by antigen retrieval to remove antibodies ...
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bioRxiv - Microbiology 2021Quote: ... the membrane was probed with the appropriate antibodies and detected with Western Lightning Plus-ECL (PerkinElmer) using a Gbox (SYNGENE) ...
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bioRxiv - Cancer Biology 2022Quote: ... Blots were probed overnight at 4 °C with indicated antibodies and developed by chemiluminescence (Perkin Elmer).
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bioRxiv - Physiology 2024Quote: ... Fluorescein was detected with a horseradish peroxidase-conjugated anti-fluorescein antibody (RRID: AB_2737388; PerkinElmer, Waltham, MA) and visualized by using the TSA Plus Fluorescein System (PerkinElmer) ...
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bioRxiv - Developmental Biology 2023Quote: ... The Lmx1a antibody detection capability was improved using the Tyramide Signal Amplification kit (TSA, Perkin Elmer), in accordance with manufacturers instructions.
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bioRxiv - Immunology 2021Quote: ... cells were pulse labelled with 50µCi of 35S-methionine and -cysteine for 1 min (EasyTag™ EXPRESS35S Protein Labeling Mix, Perkin Elmer, Waltham MA, USA) and immediately transferred into 10x volume of chase medium containing excess amounts of unlabelled cysteine (500 µg/ml) ...
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bioRxiv - Developmental Biology 2020Quote: ... Signals from anti-OAZ and Lmx1a antibodies were amplified using the Tyramide Signal Amplification Kit (Perkin Elmer), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The mean positive area was determined using appropriate isotype control antibodies and quantified using Volocity (Perkin Elmer) and Fiji (NIH ...
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bioRxiv - Neuroscience 2020Quote: ... For CC3 staining secondary antibody was followed by signal amplification using TSA Plus Cyanine 3 System (PerkinElmer). Following the incubation with the secondary antibody ...
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bioRxiv - Cancer Biology 2021Quote: ... Antigen-antibody complexes were detected with West Pico Chemi luminescent substrate using standard manufactures protocol (Perkin Elmer). Imaging of all western blots was performed using an UVP BioImager 600 system equipped with VisionWorks Life Science software (UVP ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by primary antibody and corresponding secondary horseradish peroxidase-conjugated polymer (Zytomed Systems, Germany or Perkin Elmer). Each horseradish peroxidase-conjugated polymer mediated the covalent binding of different fluorophore using tyramide signal amplification ...
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bioRxiv - Physiology 2024Quote: ... fluorescein was detected with an anti-fluorescein antibody conjugated to horseradish peroxidase (RRID: AB_2737388; PerkinElmer, Waltham, MA) and visualized using the TSA Plus Fluorescein System (PerkinElmer) ...
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bioRxiv - Neuroscience 2023Quote: ... the binding sites of the secondary antibodies were detected using Western Lightning ECL Pro (PerkinElmer, MA, USA). Chemiluminescence was visualized using the Fusion Solo S imaging system (Vilber Bio Imaging ...
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bioRxiv - Neuroscience 2023Quote: ... Fluorescein was detected with an anti-fluorescein antibody conjugated to horseradish peroxidase (RRID: AB_2737388; PerkinElmer, Waltham, MA) and visualized using the TSA Plus Fluorescein System (PerkinElmer) ...
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bioRxiv - Biochemistry 2024Quote: ... The pellets were washed with PBS pH 7.4 + 0.1% BSA and then incubated with anti-FLAG antibody conjugated to allophycocyanin (anti-FLAG:APC; PerkinElmer) (diluted 1:100 into PBS pH 7.4 + 0.1% BSA ...
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bioRxiv - Physiology 2023Quote: ... the medium was exchanged for DMEM medium supplemented with emetine and 35S-Protein Labelling Mix (35S-Met and 35S-Cys, Perkin Elmer NEG072; 100 µCi/mL). Cells were incubated for 3 h at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: DBS were acquired from consenting healthy volunteers via a finger prick onto blood sampling papers (ADVANTEC PKU-S; ADVANTEC; Whatman 903 Protein Saver Cards, Whatman; PerkinElmer 226 Spot Saver Card; PerkinElmer) and dried overnight.
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bioRxiv - Immunology 2020Quote: ... The integrated signal density corresponding to labeled antigen or antibody in each LN was calculated using LivingImage (PerkinElmer) or ImageJ software and plotted using GraphPad Prism 8 ...
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bioRxiv - Immunology 2023Quote: ... After secondary antibody incubation cells were directly imaged and analyzed using Operetta CLS High Content Analysis System (PerkinElmer).
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bioRxiv - Neuroscience 2021Quote: ... Slides were washed again and incubated with the appropriate Opal tertiary antibody (1:100 in amplification diluent, Perkin Elmer) for 60 min ...
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bioRxiv - Developmental Biology 2020Quote: ... incubated 1h in blocking buffer and then incubated overnight with an anti-DNP-POD antibody (1:1000, Perkin Elmer). The next day embryos were washed in PBS-Tween ...
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bioRxiv - Cell Biology 2023Quote: ... 10 nM full-length 6x-His-tagged human G3BP1 produced in mammalian cells was added to the reaction mix containing test compound and 0.5 nM anti-His-Tb antibody (PerkinElmer). Assays were initiated by adding 4 nM FAM-labeled PEG6-USP10 24-mer peptide probe (FAM-PEG6-GALHSPQYIFGDFSPDEFNQFFVT ...
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bioRxiv - Developmental Biology 2023Quote: ... was used to detect the anti-pH3 antibody after detection of ISH probe by using TSA plus (Perkin Elmer) green (FP1168).
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bioRxiv - Immunology 2024Quote: ... or HIS-SUMO-C-A mutant Rb 380-785 was pre-incubated with 0.4 nM HTRF donor conjugated antibodies anti-GST-Tb or anti-HIS-Tb (PerkinElmer), respectively ...
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bioRxiv - Immunology 2021Quote: ... Immunoreactive proteins were detected with either anti-mouse or anti-rabbit horseradish peroxidase-coupled secondary antibody followed by detection with enhanced chemiluminescence (PerkinElmer).
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bioRxiv - Neuroscience 2021Quote: ... Molecular weight bands were visualized by incubation with peroxidase-conjugated anti-mouse or anti-rabbit secondary antibody (1:5000; Jackson Immuno Research Laboratories) followed by treatment with the ECL-plus reagent (Western Lighting; PerkinElmer) and exposure on G:Box Chemi XRQ system (Syngene) ...
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bioRxiv - Neuroscience 2021Quote: ... and Lumi4-Tb cryptate conjugated anti-IP1 antibody (emission at 620 nm) was detected using an Envision plate reader (PerkinElmer). Results were calculated from the 665/620 nm ratio and normalised to the maximum response stimulated by acetylcholine.
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bioRxiv - Neuroscience 2022Quote: ... membranes and immunoblots were carried out using primary antibodies (Table S4) followed by incubation with appropriate secondary horseradish peroxidase-conjugated antibodies (Table S5) and chemoluminiscent detection (Western Lightning, PerkinElmer). All antibodies were diluted in PBS containing 5% semi-skimmed milk and 0.1% Tween-20 ...
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bioRxiv - Neuroscience 2020Quote: ... anti-mouse or anti-rabbit secondary antibody (1:10000; Jackson Immuno Research Laboratories) followed by treatment with ECL-plus reagent (Western Lighting, PerkinElmer) and exposure to autoradiography films ...